Human adenovirus C serotype 6 (HAdV-6) (Human adenovirus 6)
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7DY82|E7DY82_ADE06 E1B 55 kDa protein OS=Human adenovirus C serotype 6 OX=10534 GN=E1B 55K PE=3 SV=1
MM1 pKa = 7.5 RR2 pKa = 11.84 HH3 pKa = 6.51 IICHH7 pKa = 5.93 GGVITEE13 pKa = 4.22 EE14 pKa = 4.15 MAASLLDD21 pKa = 3.6 QLIEE25 pKa = 4.19 EE26 pKa = 4.48 VLADD30 pKa = 3.85 NLPPPSHH37 pKa = 7.16 FEE39 pKa = 3.8 PPTLHH44 pKa = 6.78 EE45 pKa = 5.5 LYY47 pKa = 10.58 DD48 pKa = 4.45 LDD50 pKa = 3.62 VTAPEE55 pKa = 4.89 DD56 pKa = 3.83 PNEE59 pKa = 4.13 EE60 pKa = 3.85 AVSQIFPEE68 pKa = 4.34 SVMLAVQEE76 pKa = 4.97 GIDD79 pKa = 4.38 LFTFPPAPGSPEE91 pKa = 3.95 PPHH94 pKa = 7.1 LSRR97 pKa = 11.84 QPEE100 pKa = 4.1 QPEE103 pKa = 3.86 QRR105 pKa = 11.84 ALGPVSMPNLVPEE118 pKa = 4.78 VIDD121 pKa = 3.89 LTCHH125 pKa = 5.71 EE126 pKa = 5.16 AGFPPSDD133 pKa = 4.22 DD134 pKa = 3.37 EE135 pKa = 5.17 DD136 pKa = 4.38 EE137 pKa = 4.52 EE138 pKa = 5.43 GEE140 pKa = 4.43 EE141 pKa = 4.08 FVLDD145 pKa = 4.04 YY146 pKa = 11.29 VEE148 pKa = 5.48 HH149 pKa = 7.23 PGHH152 pKa = 6.6 GCRR155 pKa = 11.84 SCHH158 pKa = 4.33 YY159 pKa = 9.82 HH160 pKa = 6.49 RR161 pKa = 11.84 RR162 pKa = 11.84 NTGDD166 pKa = 3.82 PDD168 pKa = 3.74 IMCSLCYY175 pKa = 9.84 MRR177 pKa = 11.84 TCGMFVYY184 pKa = 10.63 SKK186 pKa = 11.39
Molecular weight: 20.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.066
IPC2_protein 4.291
IPC_protein 4.228
Toseland 4.075
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.037
Rodwell 4.075
Grimsley 3.986
Solomon 4.177
Lehninger 4.126
Nozaki 4.291
DTASelect 4.418
Thurlkill 4.075
EMBOSS 4.062
Sillero 4.342
Patrickios 0.795
IPC_peptide 4.177
IPC2_peptide 4.329
IPC2.peptide.svr19 4.246
Protein with the highest isoelectric point:
>tr|E1U5P1|E1U5P1_ADE06 Isoform of Q3S8C4 Protease OS=Human adenovirus C serotype 6 OX=10534 GN=L3 PE=2 SV=1
MM1 pKa = 7.72 SKK3 pKa = 10.63 RR4 pKa = 11.84 KK5 pKa = 9.53 IKK7 pKa = 10.84 EE8 pKa = 3.51 EE9 pKa = 3.89 MLQVIAPEE17 pKa = 4.21 IYY19 pKa = 10.25 GPPKK23 pKa = 10.38 KK24 pKa = 10.29 EE25 pKa = 3.84 EE26 pKa = 3.69 QDD28 pKa = 3.54 YY29 pKa = 11.05 KK30 pKa = 10.5 PRR32 pKa = 11.84 KK33 pKa = 8.67 LKK35 pKa = 10.33 RR36 pKa = 11.84 VKK38 pKa = 10.18 KK39 pKa = 10.1 KK40 pKa = 10.88 KK41 pKa = 10.23 KK42 pKa = 10.35 DD43 pKa = 3.74 DD44 pKa = 4.83 DD45 pKa = 4.92 DD46 pKa = 6.89 DD47 pKa = 4.46 EE48 pKa = 7.23 LDD50 pKa = 4.68 DD51 pKa = 4.91 EE52 pKa = 5.32 VEE54 pKa = 4.32 LLHH57 pKa = 6.36 ATAPRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 VQWKK68 pKa = 7.55 GRR70 pKa = 11.84 RR71 pKa = 11.84 VRR73 pKa = 11.84 RR74 pKa = 11.84 VLRR77 pKa = 11.84 PGTTVVFTPGEE88 pKa = 4.05 RR89 pKa = 11.84 STRR92 pKa = 11.84 TYY94 pKa = 10.41 KK95 pKa = 10.22 RR96 pKa = 11.84 VYY98 pKa = 10.52 DD99 pKa = 3.86 EE100 pKa = 4.9 VYY102 pKa = 10.77 GDD104 pKa = 4.06 EE105 pKa = 5.51 DD106 pKa = 4.34 LLEE109 pKa = 4.21 QANEE113 pKa = 3.98 RR114 pKa = 11.84 LGEE117 pKa = 3.73 FAYY120 pKa = 10.17 GKK122 pKa = 9.09 RR123 pKa = 11.84 HH124 pKa = 6.46 KK125 pKa = 11.17 DD126 pKa = 3.29 MLALPLDD133 pKa = 4.36 EE134 pKa = 5.85 GNPTPSLKK142 pKa = 10.31 PVTLQQVLPALAPSEE157 pKa = 4.33 EE158 pKa = 3.88 KK159 pKa = 10.48 RR160 pKa = 11.84 GLKK163 pKa = 10.08 RR164 pKa = 11.84 EE165 pKa = 4.22 SGDD168 pKa = 3.66 LAPTVQLMVPKK179 pKa = 10.19 RR180 pKa = 11.84 QRR182 pKa = 11.84 LEE184 pKa = 4.07 DD185 pKa = 3.69 VLEE188 pKa = 4.36 KK189 pKa = 9.08 MTVEE193 pKa = 4.41 PGLEE197 pKa = 3.84 PEE199 pKa = 4.26 VRR201 pKa = 11.84 VRR203 pKa = 11.84 PIKK206 pKa = 10.29 QVAPGLGVQTVDD218 pKa = 3.28 VQIPTTSSTSIATATEE234 pKa = 4.06 GMEE237 pKa = 4.31 TQTSPVASAVADD249 pKa = 3.75 AAVQAAAAAASKK261 pKa = 9.84 TSTEE265 pKa = 4.18 VQTDD269 pKa = 2.66 PWMFRR274 pKa = 11.84 VSAPRR279 pKa = 11.84 RR280 pKa = 11.84 PRR282 pKa = 11.84 RR283 pKa = 11.84 SRR285 pKa = 11.84 KK286 pKa = 9.48 YY287 pKa = 8.94 GAASALLPEE296 pKa = 4.69 YY297 pKa = 10.54 ALHH300 pKa = 6.8 PSIAPTPGYY309 pKa = 9.62 RR310 pKa = 11.84 GYY312 pKa = 9.34 TYY314 pKa = 10.47 RR315 pKa = 11.84 PRR317 pKa = 11.84 RR318 pKa = 11.84 RR319 pKa = 11.84 ATTRR323 pKa = 11.84 RR324 pKa = 11.84 RR325 pKa = 11.84 TTTGTRR331 pKa = 11.84 RR332 pKa = 11.84 RR333 pKa = 11.84 RR334 pKa = 11.84 RR335 pKa = 11.84 RR336 pKa = 11.84 RR337 pKa = 11.84 QPVLAPISVRR347 pKa = 11.84 RR348 pKa = 11.84 VARR351 pKa = 11.84 EE352 pKa = 3.67 GGRR355 pKa = 11.84 TLVLPTARR363 pKa = 11.84 YY364 pKa = 8.7 HH365 pKa = 6.0 PSIVV369 pKa = 3.15
Molecular weight: 41.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.285
IPC2_protein 9.575
IPC_protein 10.145
Toseland 10.54
ProMoST 10.175
Dawson 10.643
Bjellqvist 10.321
Wikipedia 10.833
Rodwell 10.935
Grimsley 10.701
Solomon 10.716
Lehninger 10.687
Nozaki 10.496
DTASelect 10.321
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.57
Patrickios 10.599
IPC_peptide 10.73
IPC2_peptide 8.946
IPC2.peptide.svr19 8.849
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
2
20
8966
140
1056
448.3
50.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.231 ± 0.287
1.751 ± 0.329
5.365 ± 0.23
6.469 ± 0.504
3.703 ± 0.245
6.112 ± 0.244
2.331 ± 0.19
3.614 ± 0.18
3.759 ± 0.459
9.324 ± 0.408
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.61 ± 0.265
4.617 ± 0.498
6.781 ± 0.312
4.495 ± 0.28
7.45 ± 0.617
6.837 ± 0.413
5.934 ± 0.431
6.045 ± 0.264
1.26 ± 0.144
3.29 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here