Marinospirillum alkaliphilum DSM 21637

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Marinospirillum; Marinospirillum alkaliphilum

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K1YJJ5|A0A1K1YJJ5_9GAMM Lipopolysaccharide assembly protein B OS=Marinospirillum alkaliphilum DSM 21637 OX=1122209 GN=lapB PE=3 SV=1
MM1 pKa = 7.0ITEE4 pKa = 3.9QAVNQLAAYY13 pKa = 9.45IGQLPPAEE21 pKa = 3.94WDD23 pKa = 3.49DD24 pKa = 3.96RR25 pKa = 11.84LNTAFEE31 pKa = 4.2EE32 pKa = 5.07GKK34 pKa = 8.67VTLCSEE40 pKa = 3.89NDD42 pKa = 3.22IPGLAKK48 pKa = 9.93PVHH51 pKa = 5.96QGAGFDD57 pKa = 4.31LYY59 pKa = 11.47LVDD62 pKa = 5.62ASNACASLTNDD73 pKa = 3.83FSRR76 pKa = 11.84ACGVVVALHH85 pKa = 7.4DD86 pKa = 5.38DD87 pKa = 4.11DD88 pKa = 7.35DD89 pKa = 4.53DD90 pKa = 3.94

Molecular weight: 9.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K1X0V5|A0A1K1X0V5_9GAMM Sulfurtransferase OS=Marinospirillum alkaliphilum DSM 21637 OX=1122209 GN=SAMN02745752_01649 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.97GRR39 pKa = 11.84KK40 pKa = 8.69RR41 pKa = 11.84LCAA44 pKa = 3.9

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2894

0

2894

963687

39

2722

333.0

36.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.976 ± 0.048

0.947 ± 0.015

5.265 ± 0.034

6.222 ± 0.044

3.59 ± 0.031

7.336 ± 0.045

2.389 ± 0.023

4.883 ± 0.034

3.431 ± 0.041

12.612 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.019

3.062 ± 0.034

4.633 ± 0.034

5.75 ± 0.054

6.367 ± 0.035

5.592 ± 0.037

4.9 ± 0.035

6.737 ± 0.038

1.414 ± 0.021

2.422 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski