Hubei mosquito virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEJ4|A0A1L3KEJ4_9VIRU RNA-directed RNA polymerase OS=Hubei mosquito virus 2 OX=1922926 PE=4 SV=1
MM1 pKa = 7.18YY2 pKa = 10.23KK3 pKa = 9.94PYY5 pKa = 10.7TMIMEE10 pKa = 4.93DD11 pKa = 4.34LFISHH16 pKa = 6.61YY17 pKa = 11.59AMLVYY22 pKa = 10.33VALLVFLALTIVNVINPCTKK42 pKa = 10.32AVCTCSFAAVFVMFCRR58 pKa = 11.84ALALVTRR65 pKa = 11.84QFLFQGIHH73 pKa = 5.22GVVVVLTSKK82 pKa = 10.81KK83 pKa = 8.33FWQSFYY89 pKa = 10.34VTTPEE94 pKa = 4.15IFYY97 pKa = 10.76TMIAFYY103 pKa = 9.39LTLALVIAFNVWYY116 pKa = 9.98KK117 pKa = 10.36RR118 pKa = 11.84RR119 pKa = 11.84EE120 pKa = 4.16AMRR123 pKa = 11.84HH124 pKa = 4.83KK125 pKa = 10.99YY126 pKa = 8.92MVLNEE131 pKa = 4.16SGTYY135 pKa = 8.66MEE137 pKa = 5.18EE138 pKa = 3.97KK139 pKa = 10.73AMPNSHH145 pKa = 6.05YY146 pKa = 10.41EE147 pKa = 3.95IVPVLPGFQAAVLVSLDD164 pKa = 3.64GITYY168 pKa = 10.16RR169 pKa = 11.84EE170 pKa = 3.94NGQCFWVDD178 pKa = 3.29EE179 pKa = 4.46GLVTAAHH186 pKa = 5.37VVEE189 pKa = 5.87GYY191 pKa = 10.28DD192 pKa = 3.22YY193 pKa = 11.03CCIYY197 pKa = 10.52RR198 pKa = 11.84DD199 pKa = 3.81EE200 pKa = 4.17KK201 pKa = 11.24HH202 pKa = 6.54KK203 pKa = 10.97IEE205 pKa = 4.33VPSSVFEE212 pKa = 4.2TGQGDD217 pKa = 4.16YY218 pKa = 10.9ACCRR222 pKa = 11.84DD223 pKa = 4.43PIKK226 pKa = 10.1ITQKK230 pKa = 10.63VGLSKK235 pKa = 11.09AKK237 pKa = 10.18LAGVGLEE244 pKa = 4.05KK245 pKa = 10.94GSGISANIVARR256 pKa = 11.84GKK258 pKa = 10.58RR259 pKa = 11.84SIGFLDD265 pKa = 3.15QHH267 pKa = 5.93PQFGFVTYY275 pKa = 10.44SGSTTSGFSGAPYY288 pKa = 10.21HH289 pKa = 7.03LGRR292 pKa = 11.84TILGMHH298 pKa = 7.14LGADD302 pKa = 4.17SVNMGYY308 pKa = 9.9EE309 pKa = 3.71AAFLKK314 pKa = 10.33TEE316 pKa = 4.24LRR318 pKa = 11.84PSRR321 pKa = 11.84VIKK324 pKa = 10.68KK325 pKa = 10.32LVDD328 pKa = 3.84LKK330 pKa = 11.32KK331 pKa = 10.43EE332 pKa = 4.4DD333 pKa = 3.59SASWLVEE340 pKa = 3.53HH341 pKa = 7.33LGRR344 pKa = 11.84EE345 pKa = 4.18DD346 pKa = 4.85EE347 pKa = 4.17IQYY350 pKa = 8.13WQSPFDD356 pKa = 3.79PSEE359 pKa = 4.06YY360 pKa = 10.1KK361 pKa = 10.8LKK363 pKa = 10.35IGNMYY368 pKa = 10.08HH369 pKa = 7.09IVDD372 pKa = 3.8NEE374 pKa = 4.0VLKK377 pKa = 11.05EE378 pKa = 3.48ILTRR382 pKa = 11.84KK383 pKa = 9.62KK384 pKa = 10.47GRR386 pKa = 11.84QAVDD390 pKa = 3.19KK391 pKa = 10.48IDD393 pKa = 3.58YY394 pKa = 10.47LEE396 pKa = 4.83EE397 pKa = 5.31SDD399 pKa = 5.4APRR402 pKa = 11.84KK403 pKa = 5.21TTKK406 pKa = 9.52STEE409 pKa = 4.04TMVWRR414 pKa = 11.84EE415 pKa = 3.85DD416 pKa = 3.37VEE418 pKa = 4.37EE419 pKa = 4.11SVRR422 pKa = 11.84VYY424 pKa = 10.84GGEE427 pKa = 3.64VDD429 pKa = 5.62LFDD432 pKa = 4.49EE433 pKa = 4.63PVIVPVPVEE442 pKa = 4.43DD443 pKa = 3.39RR444 pKa = 11.84HH445 pKa = 6.11EE446 pKa = 4.72DD447 pKa = 3.58PLIATIRR454 pKa = 11.84GLVQEE459 pKa = 5.02FGRR462 pKa = 11.84TSVYY466 pKa = 10.44TSAQLPAVKK475 pKa = 9.69EE476 pKa = 4.05AAQVPPVKK484 pKa = 9.98EE485 pKa = 4.0AEE487 pKa = 4.33AVVPAAPRR495 pKa = 11.84VDD497 pKa = 4.3EE498 pKa = 5.32LPLAPRR504 pKa = 11.84DD505 pKa = 3.39AMTFNDD511 pKa = 3.58SGNLFRR517 pKa = 11.84APAVPAGAPGMVSVPAPVQNQGQPIYY543 pKa = 11.11GPMAFPYY550 pKa = 9.79PPPLVNYY557 pKa = 9.67HH558 pKa = 5.43MEE560 pKa = 4.27SRR562 pKa = 11.84SSMLAPQNVAFTRR575 pKa = 11.84TQRR578 pKa = 11.84NRR580 pKa = 11.84NRR582 pKa = 11.84RR583 pKa = 11.84AARR586 pKa = 11.84QQKK589 pKa = 9.87RR590 pKa = 11.84SEE592 pKa = 4.07LEE594 pKa = 3.53QYY596 pKa = 9.67RR597 pKa = 11.84QRR599 pKa = 11.84FGPLQNGGATSQQPPTPTHH618 pKa = 6.26GSIEE622 pKa = 4.42SSTGQQ627 pKa = 3.09

Molecular weight: 70.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEG1|A0A1L3KEG1_9VIRU Uncharacterized protein OS=Hubei mosquito virus 2 OX=1922926 PE=4 SV=1
MM1 pKa = 7.57SKK3 pKa = 9.99QLKK6 pKa = 9.15KK7 pKa = 10.56QLNALTAQVAALKK20 pKa = 10.37VGKK23 pKa = 8.41QQQPAPAKK31 pKa = 9.68RR32 pKa = 11.84KK33 pKa = 9.38RR34 pKa = 11.84NRR36 pKa = 11.84KK37 pKa = 9.06RR38 pKa = 11.84KK39 pKa = 9.65GPSPLSNTGISSDD52 pKa = 3.0GRR54 pKa = 11.84IVVSRR59 pKa = 11.84CEE61 pKa = 3.82LLFEE65 pKa = 4.42VTVDD69 pKa = 3.43AAKK72 pKa = 10.75DD73 pKa = 3.42SAAFYY78 pKa = 10.96YY79 pKa = 10.35VLQASATRR87 pKa = 11.84MPWLSKK93 pKa = 9.63LAKK96 pKa = 10.07NFSQIVWRR104 pKa = 11.84SARR107 pKa = 11.84LEE109 pKa = 3.8YY110 pKa = 10.48RR111 pKa = 11.84PAVGTMKK118 pKa = 10.68DD119 pKa = 3.22GSLVIGIDD127 pKa = 3.53WNPVASAPDD136 pKa = 3.52KK137 pKa = 11.42AKK139 pKa = 10.41VQSMTPNFQVPVWQKK154 pKa = 11.19KK155 pKa = 8.07EE156 pKa = 3.8LTIPSQRR163 pKa = 11.84LMSRR167 pKa = 11.84KK168 pKa = 9.7YY169 pKa = 10.26YY170 pKa = 9.94STDD173 pKa = 2.78SSSNVQLVDD182 pKa = 3.66KK183 pKa = 10.03VPCEE187 pKa = 3.75VLGYY191 pKa = 9.37LSCTADD197 pKa = 4.34DD198 pKa = 4.16KK199 pKa = 11.37NKK201 pKa = 10.52QYY203 pKa = 10.97FGDD206 pKa = 3.29IWIHH210 pKa = 5.69YY211 pKa = 8.91NVILQGPQXGSEE223 pKa = 4.46EE224 pKa = 4.56PPPPPPPPPPPPAPTVFNYY243 pKa = 10.57NVVRR247 pKa = 11.84DD248 pKa = 3.81VTQYY252 pKa = 10.56EE253 pKa = 3.91IRR255 pKa = 11.84GSVRR259 pKa = 11.84TIFKK263 pKa = 10.84DD264 pKa = 3.5RR265 pKa = 11.84MAPYY269 pKa = 9.84PYY271 pKa = 8.8DD272 pKa = 3.64TNVFWPEE279 pKa = 3.29EE280 pKa = 4.03TVILNRR286 pKa = 11.84DD287 pKa = 3.65LIEE290 pKa = 4.08YY291 pKa = 10.12SGSDD295 pKa = 3.15NVAVNGKK302 pKa = 9.6DD303 pKa = 3.45FNYY306 pKa = 9.61VDD308 pKa = 4.06QYY310 pKa = 11.84AVSADD315 pKa = 3.84RR316 pKa = 11.84QEE318 pKa = 5.2DD319 pKa = 3.69GHH321 pKa = 6.03EE322 pKa = 4.26TTLEE326 pKa = 4.59LISRR330 pKa = 11.84PPYY333 pKa = 9.64SDD335 pKa = 2.92KK336 pKa = 11.06VPYY339 pKa = 10.07GVLDD343 pKa = 3.2ITISIRR349 pKa = 11.84SPHH352 pKa = 5.94FPRR355 pKa = 11.84LAMYY359 pKa = 8.86EE360 pKa = 4.09CAIQFVADD368 pKa = 4.21RR369 pKa = 11.84NIFDD373 pKa = 3.74SQQIRR378 pKa = 11.84QIDD381 pKa = 3.64VAFDD385 pKa = 3.19KK386 pKa = 10.79TKK388 pKa = 10.44EE389 pKa = 3.7IRR391 pKa = 11.84LVGDD395 pKa = 3.08VLNVYY400 pKa = 8.11RR401 pKa = 11.84TTNRR405 pKa = 11.84RR406 pKa = 11.84LDD408 pKa = 3.31NWYY411 pKa = 9.06MIKK414 pKa = 10.22FCQVLEE420 pKa = 4.09ILDD423 pKa = 3.54PRR425 pKa = 11.84VAVRR429 pKa = 11.84VRR431 pKa = 11.84VRR433 pKa = 11.84INRR436 pKa = 11.84LGKK439 pKa = 10.12HH440 pKa = 5.92NDD442 pKa = 3.52QIDD445 pKa = 3.55MGTKK449 pKa = 10.18LDD451 pKa = 3.76ITPVLSWYY459 pKa = 9.68TSVTKK464 pKa = 10.24FLGVGEE470 pKa = 4.55GSDD473 pKa = 3.59DD474 pKa = 3.7GSRR477 pKa = 11.84SPEE480 pKa = 3.84LEE482 pKa = 3.61GSYY485 pKa = 10.43EE486 pKa = 4.08ILDD489 pKa = 4.49DD490 pKa = 4.58PPPSGSSS497 pKa = 3.01

Molecular weight: 55.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1475

351

627

491.7

55.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.644 ± 0.753

1.559 ± 0.32

5.492 ± 0.824

5.288 ± 0.705

4.203 ± 0.507

6.034 ± 0.423

1.559 ± 0.363

4.949 ± 0.276

5.356 ± 0.281

8.0 ± 0.772

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.508 ± 0.306

3.593 ± 0.394

6.712 ± 0.601

4.881 ± 0.219

6.034 ± 0.266

6.508 ± 0.64

5.017 ± 0.3

9.288 ± 0.223

1.831 ± 0.598

4.475 ± 0.08
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski