Murmansk poxvirus
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 202 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A223FMM8|A0A223FMM8_9POXV Substrate for poxvirus S-S bond formation pathway OS=Murmansk poxvirus OX=2025359 GN=Murmansk-043 PE=4 SV=1
MM1 pKa = 7.74 DD2 pKa = 4.31 TNSLYY7 pKa = 10.95 EE8 pKa = 3.85 EE9 pKa = 4.75 GMNISNDD16 pKa = 2.96 LDD18 pKa = 4.34 SIVNLLEE25 pKa = 3.75 MRR27 pKa = 11.84 CIDD30 pKa = 3.73 SSNYY34 pKa = 8.41 NDD36 pKa = 4.65 KK37 pKa = 10.92 YY38 pKa = 11.05 LPNNFTDD45 pKa = 3.82 CTYY48 pKa = 11.62 KK49 pKa = 10.97 LVDD52 pKa = 3.45 NKK54 pKa = 10.05 EE55 pKa = 3.98 FKK57 pKa = 10.94 YY58 pKa = 11.13 VIDD61 pKa = 3.66 SFINMVEE68 pKa = 3.89 NDD70 pKa = 3.48 NGEE73 pKa = 4.58 TISSLSNFINVLKK86 pKa = 10.71 NINNEE91 pKa = 3.68 EE92 pKa = 4.15 SVQLVEE98 pKa = 5.94 FSKK101 pKa = 11.19 SLNKK105 pKa = 10.3 LKK107 pKa = 10.66 NLSGEE112 pKa = 4.27 EE113 pKa = 3.89 FTSQLKK119 pKa = 10.51 NIIYY123 pKa = 9.86 LVKK126 pKa = 10.23 EE127 pKa = 4.16 DD128 pKa = 3.99 TSGEE132 pKa = 4.54 LITIMSNFIDD142 pKa = 3.68 SCNMVKK148 pKa = 10.68 YY149 pKa = 8.96 ITSEE153 pKa = 4.01 EE154 pKa = 4.1 IEE156 pKa = 4.15 NLLTDD161 pKa = 4.76 FINNIDD167 pKa = 4.85 DD168 pKa = 3.72 IDD170 pKa = 4.35 NEE172 pKa = 4.5 LVVKK176 pKa = 10.67
Molecular weight: 20.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.151
IPC2_protein 4.024
IPC_protein 3.986
Toseland 3.795
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.846
Rodwell 3.808
Grimsley 3.706
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.24
Thurlkill 3.821
EMBOSS 3.859
Sillero 4.101
Patrickios 3.338
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 4.01
Protein with the highest isoelectric point:
>tr|A0A223FMX1|A0A223FMX1_9POXV DNA helicase OS=Murmansk poxvirus OX=2025359 GN=Murmansk-134 PE=4 SV=1
MM1 pKa = 7.2 SWYY4 pKa = 9.57 EE5 pKa = 3.8 RR6 pKa = 11.84 YY7 pKa = 10.54 NIVLNPPKK15 pKa = 10.29 RR16 pKa = 11.84 CSKK19 pKa = 10.7 CNEE22 pKa = 3.94 NLMTLLADD30 pKa = 4.3 DD31 pKa = 4.79 GNNIRR36 pKa = 11.84 MILYY40 pKa = 7.99 SQPNKK45 pKa = 10.7 LKK47 pKa = 10.46 ILRR50 pKa = 11.84 DD51 pKa = 3.77 FLATSRR57 pKa = 11.84 NKK59 pKa = 9.45 MFLYY63 pKa = 10.37 KK64 pKa = 10.25 ILDD67 pKa = 3.58 NEE69 pKa = 4.18 VRR71 pKa = 11.84 RR72 pKa = 11.84 VLTT75 pKa = 3.72
Molecular weight: 9.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.187
IPC2_protein 9.121
IPC_protein 9.165
Toseland 9.692
ProMoST 9.472
Dawson 9.955
Bjellqvist 9.692
Wikipedia 10.145
Rodwell 10.233
Grimsley 10.028
Solomon 10.014
Lehninger 9.984
Nozaki 9.794
DTASelect 9.663
Thurlkill 9.794
EMBOSS 10.116
Sillero 9.897
Patrickios 7.585
IPC_peptide 10.014
IPC2_peptide 8.536
IPC2.peptide.svr19 8.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
202
0
202
62817
33
1875
311.0
35.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.291 ± 0.118
2.135 ± 0.087
6.805 ± 0.145
5.457 ± 0.146
4.577 ± 0.124
3.884 ± 0.106
2.015 ± 0.067
10.064 ± 0.162
8.082 ± 0.158
8.555 ± 0.143
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.566 ± 0.069
7.812 ± 0.168
3.171 ± 0.131
1.999 ± 0.061
3.911 ± 0.114
7.549 ± 0.154
5.881 ± 0.136
5.908 ± 0.111
0.708 ± 0.043
5.629 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here