Klebsiella phage SH-Kp 152410
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9VGZ2|A0A2K9VGZ2_9CAUD HNHc domain-containing protein OS=Klebsiella phage SH-Kp 152410 OX=2066504 GN=2410_orf00015 PE=4 SV=1
MM1 pKa = 7.4 NPSDD5 pKa = 3.87 WCRR8 pKa = 11.84 EE9 pKa = 4.05 MYY11 pKa = 10.1 EE12 pKa = 4.17 KK13 pKa = 10.16 TLDD16 pKa = 3.46 PAYY19 pKa = 9.07 ITLYY23 pKa = 11.1 NMWKK27 pKa = 9.04 EE28 pKa = 3.82 WEE30 pKa = 4.06 DD31 pKa = 3.21 AKK33 pKa = 11.1 FVVTVEE39 pKa = 4.22 TANASYY45 pKa = 10.04 EE46 pKa = 4.28 LPVHH50 pKa = 6.68 AGSLEE55 pKa = 3.95 EE56 pKa = 4.04 ALEE59 pKa = 4.21 VAEE62 pKa = 5.48 AEE64 pKa = 4.25 YY65 pKa = 11.2 EE66 pKa = 4.05 EE67 pKa = 5.11 LGQVTRR73 pKa = 11.84 VRR75 pKa = 11.84 PDD77 pKa = 2.73 SHH79 pKa = 7.55
Molecular weight: 9.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.224
IPC2_protein 4.38
IPC_protein 4.228
Toseland 4.101
ProMoST 4.304
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.012
Rodwell 4.075
Grimsley 4.012
Solomon 4.151
Lehninger 4.101
Nozaki 4.279
DTASelect 4.355
Thurlkill 4.101
EMBOSS 4.024
Sillero 4.342
Patrickios 2.969
IPC_peptide 4.164
IPC2_peptide 4.329
IPC2.peptide.svr19 4.257
Protein with the highest isoelectric point:
>tr|A0A2K9VGX8|A0A2K9VGX8_9CAUD Internal virion protein gp14 OS=Klebsiella phage SH-Kp 152410 OX=2066504 GN=2410_orf00037 PE=3 SV=1
MM1 pKa = 7.42 IKK3 pKa = 10.61 YY4 pKa = 10.27 GITLEE9 pKa = 3.94 DD10 pKa = 3.01 LKK12 pKa = 11.18 YY13 pKa = 10.3 YY14 pKa = 10.63 RR15 pKa = 11.84 FALMHH20 pKa = 6.54 GKK22 pKa = 8.27 HH23 pKa = 6.49 HH24 pKa = 7.75 DD25 pKa = 3.81 YY26 pKa = 11.59 LMAALRR32 pKa = 11.84 QTKK35 pKa = 8.75 WGYY38 pKa = 11.3 SMSLRR43 pKa = 11.84 IDD45 pKa = 3.28 TRR47 pKa = 11.84 HH48 pKa = 5.6 IRR50 pKa = 11.84 AAQLAMAYY58 pKa = 9.22 GASDD62 pKa = 3.87 ALTKK66 pKa = 10.57 RR67 pKa = 11.84 ILTKK71 pKa = 9.95 HH72 pKa = 6.13 RR73 pKa = 11.84 KK74 pKa = 5.46 MTARR78 pKa = 11.84 QAACAVKK85 pKa = 9.55 WARR88 pKa = 11.84 LTLLSYY94 pKa = 10.47 QQ95 pKa = 3.58
Molecular weight: 11.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.289
IPC2_protein 9.955
IPC_protein 10.452
Toseland 10.394
ProMoST 10.116
Dawson 10.584
Bjellqvist 10.292
Wikipedia 10.789
Rodwell 10.965
Grimsley 10.672
Solomon 10.628
Lehninger 10.584
Nozaki 10.379
DTASelect 10.277
Thurlkill 10.438
EMBOSS 10.804
Sillero 10.496
Patrickios 10.657
IPC_peptide 10.628
IPC2_peptide 9.151
IPC2.peptide.svr19 8.493
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
1
48
12204
36
1322
254.3
28.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.005 ± 0.47
0.967 ± 0.155
6.4 ± 0.184
6.735 ± 0.457
3.614 ± 0.193
8.014 ± 0.336
1.942 ± 0.203
5.023 ± 0.153
6.85 ± 0.368
8.12 ± 0.291
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.663 ± 0.194
4.351 ± 0.275
3.728 ± 0.181
4.072 ± 0.355
5.31 ± 0.192
6.088 ± 0.31
5.457 ± 0.301
6.695 ± 0.362
1.409 ± 0.163
3.556 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here