Alphaproteobacteria bacterium GH1-50

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; unclassified Alphaproteobacteria

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C9IIT4|A0A7C9IIT4_9PROT Uncharacterized protein OS=Alphaproteobacteria bacterium GH1-50 OX=2691042 GN=GQ651_17015 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.26KK3 pKa = 10.31VLLASTALVLSAGVAAADD21 pKa = 3.69VAVSGDD27 pKa = 2.92GRR29 pKa = 11.84MGVANNFTGDD39 pKa = 3.65LVFSSRR45 pKa = 11.84IRR47 pKa = 11.84IAFSASGEE55 pKa = 4.24TDD57 pKa = 2.76GGLSFGGSIRR67 pKa = 11.84ADD69 pKa = 3.1NAGGGEE75 pKa = 4.28GGTAGSVFIEE85 pKa = 4.64GAFGKK90 pKa = 10.63LSMGDD95 pKa = 2.98VDD97 pKa = 4.5SAAEE101 pKa = 3.86AAVGDD106 pKa = 4.08VSGVGYY112 pKa = 10.31QLAGRR117 pKa = 11.84QEE119 pKa = 4.4SIYY122 pKa = 10.53IGGPATAALYY132 pKa = 9.5EE133 pKa = 4.35YY134 pKa = 10.76SAGDD138 pKa = 3.39LSVYY142 pKa = 10.5LGAGQQEE149 pKa = 4.48TGNDD153 pKa = 3.62SVSVGLAYY161 pKa = 10.77SFGNFGVSAGYY172 pKa = 7.49EE173 pKa = 4.28TNDD176 pKa = 3.49SVGAAPDD183 pKa = 3.23TATNVDD189 pKa = 4.56HH190 pKa = 7.42IILGATAAFGDD201 pKa = 3.74ATLKK205 pKa = 10.52VIHH208 pKa = 6.49GMADD212 pKa = 3.41FDD214 pKa = 5.91GGDD217 pKa = 3.64ADD219 pKa = 3.84QTAVSLDD226 pKa = 3.69YY227 pKa = 11.89VMGATTITAMYY238 pKa = 9.37YY239 pKa = 10.4DD240 pKa = 4.04EE241 pKa = 5.74SEE243 pKa = 4.33FGALGSGEE251 pKa = 4.23GFGLGVAYY259 pKa = 10.3DD260 pKa = 4.01LGGGATLKK268 pKa = 10.68AGYY271 pKa = 9.9ISDD274 pKa = 3.85EE275 pKa = 4.23TDD277 pKa = 2.82GATEE281 pKa = 4.62DD282 pKa = 4.94GFDD285 pKa = 4.18LGINMSFF292 pKa = 3.47

Molecular weight: 28.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C9MU44|A0A7C9MU44_9PROT Uncharacterized protein OS=Alphaproteobacteria bacterium GH1-50 OX=2691042 GN=GQ651_02150 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3643

0

3643

1110986

34

2536

305.0

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.54 ± 0.064

0.794 ± 0.013

6.365 ± 0.046

6.223 ± 0.038

3.783 ± 0.027

8.906 ± 0.039

1.947 ± 0.021

5.156 ± 0.029

2.815 ± 0.031

9.934 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.654 ± 0.02

2.365 ± 0.023

5.107 ± 0.029

2.694 ± 0.022

7.056 ± 0.043

5.1 ± 0.026

5.55 ± 0.03

7.441 ± 0.033

1.434 ± 0.017

2.138 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski