Amino acid dipepetide frequency for Alphaproteobacteria bacterium GH1-50

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.166AlaAla: 17.166 ± 0.175	0.994AlaCys: 0.994 ± 0.033	7.296AlaAsp: 7.296 ± 0.082	8.946AlaGlu: 8.946 ± 0.106	4.7AlaPhe: 4.7 ± 0.08	10.915AlaGly: 10.915 ± 0.116	2.351AlaHis: 2.351 ± 0.046	6.071AlaIle: 6.071 ± 0.089	3.32AlaLys: 3.32 ± 0.071	14.018AlaLeu: 14.018 ± 0.142	3.774AlaMet: 3.774 ± 0.067	2.52AlaAsn: 2.52 ± 0.045	5.904AlaPro: 5.904 ± 0.082	3.302AlaGln: 3.302 ± 0.052	9.477AlaArg: 9.477 ± 0.124	5.813AlaSer: 5.813 ± 0.076	6.08AlaThr: 6.08 ± 0.08	8.674AlaVal: 8.674 ± 0.1	1.53AlaTrp: 1.53 ± 0.043	2.545AlaTyr: 2.545 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.833CysAla: 0.833 ± 0.029	0.088CysCys: 0.088 ± 0.009	0.557CysAsp: 0.557 ± 0.024	0.395CysGlu: 0.395 ± 0.019	0.295CysPhe: 0.295 ± 0.017	0.864CysGly: 0.864 ± 0.029	0.261CysHis: 0.261 ± 0.017	0.374CysIle: 0.374 ± 0.019	0.142CysLys: 0.142 ± 0.01	0.842CysLeu: 0.842 ± 0.027	0.157CysMet: 0.157 ± 0.011	0.19CysAsn: 0.19 ± 0.012	0.51CysPro: 0.51 ± 0.021	0.218CysGln: 0.218 ± 0.015	0.587CysArg: 0.587 ± 0.023	0.394CysSer: 0.394 ± 0.017	0.392CysThr: 0.392 ± 0.021	0.545CysVal: 0.545 ± 0.025	0.107CysTrp: 0.107 ± 0.009	0.184CysTyr: 0.184 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	8.04AspAla: 8.04 ± 0.094	0.475AspCys: 0.475 ± 0.023	3.888AspAsp: 3.888 ± 0.078	4.138AspGlu: 4.138 ± 0.069	2.383AspPhe: 2.383 ± 0.047	6.186AspGly: 6.186 ± 0.098	1.319AspHis: 1.319 ± 0.039	3.436AspIle: 3.436 ± 0.057	1.559AspLys: 1.559 ± 0.036	6.337AspLeu: 6.337 ± 0.089	1.761AspMet: 1.761 ± 0.037	1.144AspAsn: 1.144 ± 0.033	3.727AspPro: 3.727 ± 0.065	1.698AspGln: 1.698 ± 0.045	4.966AspArg: 4.966 ± 0.061	2.098AspSer: 2.098 ± 0.044	3.148AspThr: 3.148 ± 0.071	4.533AspVal: 4.533 ± 0.067	1.267AspTrp: 1.267 ± 0.036	1.544AspTyr: 1.544 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	9.1GluAla: 9.1 ± 0.108	0.362GluCys: 0.362 ± 0.019	3.827GluAsp: 3.827 ± 0.065	3.867GluGlu: 3.867 ± 0.082	1.831GluPhe: 1.831 ± 0.047	5.256GluGly: 5.256 ± 0.076	1.155GluHis: 1.155 ± 0.036	3.699GluIle: 3.699 ± 0.063	2.088GluLys: 2.088 ± 0.057	5.006GluLeu: 5.006 ± 0.074	1.867GluMet: 1.867 ± 0.045	1.691GluAsn: 1.691 ± 0.036	2.721GluPro: 2.721 ± 0.063	1.715GluGln: 1.715 ± 0.044	5.007GluArg: 5.007 ± 0.072	2.243GluSer: 2.243 ± 0.052	4.39GluThr: 4.39 ± 0.07	4.579GluVal: 4.579 ± 0.07	0.77GluTrp: 0.77 ± 0.024	1.056GluTyr: 1.056 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.472PheAla: 4.472 ± 0.069	0.412PheCys: 0.412 ± 0.018	2.944PheAsp: 2.944 ± 0.055	2.347PheGlu: 2.347 ± 0.049	1.566PhePhe: 1.566 ± 0.046	3.837PheGly: 3.837 ± 0.07	0.773PheHis: 0.773 ± 0.028	1.629PheIle: 1.629 ± 0.04	0.802PheLys: 0.802 ± 0.028	3.708PheLeu: 3.708 ± 0.081	0.797PheMet: 0.797 ± 0.027	0.96PheAsn: 0.96 ± 0.029	1.649PhePro: 1.649 ± 0.036	1.032PheGln: 1.032 ± 0.03	2.514PheArg: 2.514 ± 0.049	2.029PheSer: 2.029 ± 0.046	2.067PheThr: 2.067 ± 0.047	2.75PheVal: 2.75 ± 0.048	0.66PheTrp: 0.66 ± 0.029	0.879PheTyr: 0.879 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	10.356GlyAla: 10.356 ± 0.129	0.793GlyCys: 0.793 ± 0.026	5.239GlyAsp: 5.239 ± 0.083	5.061GlyGlu: 5.061 ± 0.074	3.709GlyPhe: 3.709 ± 0.059	7.915GlyGly: 7.915 ± 0.117	1.924GlyHis: 1.924 ± 0.05	4.564GlyIle: 4.564 ± 0.068	2.865GlyLys: 2.865 ± 0.058	9.347GlyLeu: 9.347 ± 0.116	2.495GlyMet: 2.495 ± 0.056	2.031GlyAsn: 2.031 ± 0.048	3.905GlyPro: 3.905 ± 0.059	3.016GlyGln: 3.016 ± 0.054	6.329GlyArg: 6.329 ± 0.079	4.147GlySer: 4.147 ± 0.072	4.866GlyThr: 4.866 ± 0.081	6.662GlyVal: 6.662 ± 0.082	1.577GlyTrp: 1.577 ± 0.036	2.261GlyTyr: 2.261 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.273HisAla: 2.273 ± 0.041	0.21HisCys: 0.21 ± 0.014	1.228HisAsp: 1.228 ± 0.034	1.072HisGlu: 1.072 ± 0.03	0.811HisPhe: 0.811 ± 0.028	1.856HisGly: 1.856 ± 0.044	0.536HisHis: 0.536 ± 0.025	0.984HisIle: 0.984 ± 0.034	0.465HisLys: 0.465 ± 0.021	2.022HisLeu: 2.022 ± 0.044	0.517HisMet: 0.517 ± 0.022	0.413HisAsn: 0.413 ± 0.018	1.299HisPro: 1.299 ± 0.039	0.512HisGln: 0.512 ± 0.024	1.312HisArg: 1.312 ± 0.035	0.818HisSer: 0.818 ± 0.023	0.745HisThr: 0.745 ± 0.029	1.564HisVal: 1.564 ± 0.041	0.323HisTrp: 0.323 ± 0.018	0.511HisTyr: 0.511 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.039IleAla: 7.039 ± 0.087	0.513IleCys: 0.513 ± 0.02	3.729IleAsp: 3.729 ± 0.07	3.881IleGlu: 3.881 ± 0.056	1.79IlePhe: 1.79 ± 0.044	4.942IleGly: 4.942 ± 0.079	0.892IleHis: 0.892 ± 0.03	2.114IleIle: 2.114 ± 0.045	1.101IleLys: 1.101 ± 0.033	5.023IleLeu: 5.023 ± 0.077	1.04IleMet: 1.04 ± 0.035	1.185IleAsn: 1.185 ± 0.034	2.332IlePro: 2.332 ± 0.045	1.079IleGln: 1.079 ± 0.035	3.513IleArg: 3.513 ± 0.054	2.834IleSer: 2.834 ± 0.054	2.677IleThr: 2.677 ± 0.051	4.114IleVal: 4.114 ± 0.069	0.69IleTrp: 0.69 ± 0.028	1.075IleTyr: 1.075 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.722LysAla: 3.722 ± 0.075	0.158LysCys: 0.158 ± 0.014	1.66LysAsp: 1.66 ± 0.04	1.491LysGlu: 1.491 ± 0.047	0.744LysPhe: 0.744 ± 0.029	2.65LysGly: 2.65 ± 0.059	0.501LysHis: 0.501 ± 0.023	1.468LysIle: 1.468 ± 0.046	1.091LysLys: 1.091 ± 0.038	2.499LysLeu: 2.499 ± 0.054	0.731LysMet: 0.731 ± 0.029	0.663LysAsn: 0.663 ± 0.026	1.602LysPro: 1.602 ± 0.041	0.669LysGln: 0.669 ± 0.026	2.06LysArg: 2.06 ± 0.046	1.672LysSer: 1.672 ± 0.045	1.819LysThr: 1.819 ± 0.042	2.041LysVal: 2.041 ± 0.053	0.354LysTrp: 0.354 ± 0.018	0.558LysTyr: 0.558 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	12.971LeuAla: 12.971 ± 0.143	0.798LeuCys: 0.798 ± 0.027	6.608LeuAsp: 6.608 ± 0.085	5.281LeuGlu: 5.281 ± 0.076	3.625LeuPhe: 3.625 ± 0.07	8.576LeuGly: 8.576 ± 0.1	1.636LeuHis: 1.636 ± 0.039	5.257LeuIle: 5.257 ± 0.081	3.16LeuLys: 3.16 ± 0.066	8.713LeuLeu: 8.713 ± 0.128	2.545LeuMet: 2.545 ± 0.048	2.464LeuAsn: 2.464 ± 0.047	5.408LeuPro: 5.408 ± 0.068	2.269LeuGln: 2.269 ± 0.043	6.501LeuArg: 6.501 ± 0.087	6.828LeuSer: 6.828 ± 0.092	6.33LeuThr: 6.33 ± 0.085	7.04LeuVal: 7.04 ± 0.088	1.331LeuTrp: 1.331 ± 0.038	2.001LeuTyr: 2.001 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	3.315MetAla: 3.315 ± 0.059	0.171MetCys: 0.171 ± 0.012	1.364MetAsp: 1.364 ± 0.04	1.317MetGlu: 1.317 ± 0.034	0.788MetPhe: 0.788 ± 0.031	2.275MetGly: 2.275 ± 0.048	0.418MetHis: 0.418 ± 0.018	1.455MetIle: 1.455 ± 0.039	0.935MetLys: 0.935 ± 0.029	2.398MetLeu: 2.398 ± 0.045	0.686MetMet: 0.686 ± 0.025	0.838MetAsn: 0.838 ± 0.031	1.505MetPro: 1.505 ± 0.036	0.819MetGln: 0.819 ± 0.03	1.857MetArg: 1.857 ± 0.045	1.85MetSer: 1.85 ± 0.041	2.149MetThr: 2.149 ± 0.048	1.834MetVal: 1.834 ± 0.036	0.226MetTrp: 0.226 ± 0.016	0.345MetTyr: 0.345 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.948AsnAla: 2.948 ± 0.05	0.24AsnCys: 0.24 ± 0.015	1.343AsnAsp: 1.343 ± 0.04	1.237AsnGlu: 1.237 ± 0.032	0.889AsnPhe: 0.889 ± 0.027	2.206AsnGly: 2.206 ± 0.048	0.5AsnHis: 0.5 ± 0.022	1.28AsnIle: 1.28 ± 0.033	0.533AsnLys: 0.533 ± 0.022	2.205AsnLeu: 2.205 ± 0.049	0.635AsnMet: 0.635 ± 0.024	0.601AsnAsn: 0.601 ± 0.028	1.687AsnPro: 1.687 ± 0.042	0.578AsnGln: 0.578 ± 0.024	1.671AsnArg: 1.671 ± 0.04	1.056AsnSer: 1.056 ± 0.03	1.202AsnThr: 1.202 ± 0.028	1.814AsnVal: 1.814 ± 0.034	0.421AsnTrp: 0.421 ± 0.019	0.603AsnTyr: 0.603 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	5.676ProAla: 5.676 ± 0.084	0.325ProCys: 0.325 ± 0.018	4.269ProAsp: 4.269 ± 0.069	4.416ProGlu: 4.416 ± 0.071	2.089ProPhe: 2.089 ± 0.047	4.734ProGly: 4.734 ± 0.067	0.954ProHis: 0.954 ± 0.029	2.286ProIle: 2.286 ± 0.048	1.464ProLys: 1.464 ± 0.044	4.548ProLeu: 4.548 ± 0.07	1.257ProMet: 1.257 ± 0.035	1.251ProAsn: 1.251 ± 0.034	2.353ProPro: 2.353 ± 0.052	1.291ProGln: 1.291 ± 0.038	2.916ProArg: 2.916 ± 0.051	2.635ProSer: 2.635 ± 0.048	2.442ProThr: 2.442 ± 0.048	4.402ProVal: 4.402 ± 0.07	0.687ProTrp: 0.687 ± 0.027	1.073ProTyr: 1.073 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.392GlnAla: 3.392 ± 0.063	0.178GlnCys: 0.178 ± 0.013	1.484GlnAsp: 1.484 ± 0.033	1.347GlnGlu: 1.347 ± 0.038	0.919GlnPhe: 0.919 ± 0.028	2.188GlnGly: 2.188 ± 0.043	0.472GlnHis: 0.472 ± 0.021	1.704GlnIle: 1.704 ± 0.039	0.942GlnLys: 0.942 ± 0.03	2.267GlnLeu: 2.267 ± 0.044	0.86GlnMet: 0.86 ± 0.029	0.818GlnAsn: 0.818 ± 0.026	1.42GlnPro: 1.42 ± 0.036	0.808GlnGln: 0.808 ± 0.035	1.907GlnArg: 1.907 ± 0.045	1.631GlnSer: 1.631 ± 0.037	1.652GlnThr: 1.652 ± 0.038	2.114GlnVal: 2.114 ± 0.045	0.348GlnTrp: 0.348 ± 0.017	0.483GlnTyr: 0.483 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.668ArgAla: 8.668 ± 0.114	0.439ArgCys: 0.439 ± 0.021	4.585ArgAsp: 4.585 ± 0.066	4.178ArgGlu: 4.178 ± 0.069	2.836ArgPhe: 2.836 ± 0.052	4.777ArgGly: 4.777 ± 0.074	1.561ArgHis: 1.561 ± 0.035	4.122ArgIle: 4.122 ± 0.07	2.071ArgLys: 2.071 ± 0.045	7.878ArgLeu: 7.878 ± 0.099	2.005ArgMet: 2.005 ± 0.041	1.736ArgAsn: 1.736 ± 0.039	3.619ArgPro: 3.619 ± 0.063	2.316ArgGln: 2.316 ± 0.048	5.555ArgArg: 5.555 ± 0.099	3.278ArgSer: 3.278 ± 0.059	3.291ArgThr: 3.291 ± 0.058	5.17ArgVal: 5.17 ± 0.079	0.949ArgTrp: 0.949 ± 0.032	1.52ArgTyr: 1.52 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	5.794SerAla: 5.794 ± 0.074	0.35SerCys: 0.35 ± 0.018	3.538SerAsp: 3.538 ± 0.058	3.2SerGlu: 3.2 ± 0.057	2.214SerPhe: 2.214 ± 0.044	5.457SerGly: 5.457 ± 0.087	1.03SerHis: 1.03 ± 0.03	2.446SerIle: 2.446 ± 0.045	1.323SerLys: 1.323 ± 0.04	5.048SerLeu: 5.048 ± 0.074	1.293SerMet: 1.293 ± 0.036	1.205SerAsn: 1.205 ± 0.029	2.541SerPro: 2.541 ± 0.055	1.333SerGln: 1.333 ± 0.037	3.406SerArg: 3.406 ± 0.061	2.477SerSer: 2.477 ± 0.052	2.509SerThr: 2.509 ± 0.047	3.971SerVal: 3.971 ± 0.06	0.653SerTrp: 0.653 ± 0.027	1.206SerTyr: 1.206 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	6.43ThrAla: 6.43 ± 0.087	0.473ThrCys: 0.473 ± 0.024	3.47ThrAsp: 3.47 ± 0.061	3.358ThrGlu: 3.358 ± 0.055	2.087ThrPhe: 2.087 ± 0.049	5.69ThrGly: 5.69 ± 0.081	1.024ThrHis: 1.024 ± 0.027	2.792ThrIle: 2.792 ± 0.058	1.372ThrLys: 1.372 ± 0.037	6.152ThrLeu: 6.152 ± 0.082	1.233ThrMet: 1.233 ± 0.035	1.194ThrAsn: 1.194 ± 0.037	3.402ThrPro: 3.402 ± 0.057	1.32ThrGln: 1.32 ± 0.037	3.591ThrArg: 3.591 ± 0.061	2.696ThrSer: 2.696 ± 0.048	2.808ThrThr: 2.808 ± 0.061	4.464ThrVal: 4.464 ± 0.067	0.737ThrTrp: 0.737 ± 0.028	1.207ThrTyr: 1.207 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	9.379ValAla: 9.379 ± 0.109	0.602ValCys: 0.602 ± 0.024	4.241ValAsp: 4.241 ± 0.07	4.683ValGlu: 4.683 ± 0.066	3.076ValPhe: 3.076 ± 0.059	5.62ValGly: 5.62 ± 0.088	1.307ValHis: 1.307 ± 0.035	4.273ValIle: 4.273 ± 0.069	1.977ValLys: 1.977 ± 0.054	7.492ValLeu: 7.492 ± 0.093	2.048ValMet: 2.048 ± 0.041	1.802ValAsn: 1.802 ± 0.037	3.726ValPro: 3.726 ± 0.057	1.803ValGln: 1.803 ± 0.04	4.62ValArg: 4.62 ± 0.069	4.426ValSer: 4.426 ± 0.071	5.032ValThr: 5.032 ± 0.07	5.685ValVal: 5.685 ± 0.085	1.102ValTrp: 1.102 ± 0.033	1.516ValTyr: 1.516 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.405TrpAla: 1.405 ± 0.041	0.14TrpCys: 0.14 ± 0.011	0.82TrpAsp: 0.82 ± 0.031	0.729TrpGlu: 0.729 ± 0.025	0.56TrpPhe: 0.56 ± 0.024	1.107TrpGly: 1.107 ± 0.034	0.352TrpHis: 0.352 ± 0.019	0.742TrpIle: 0.742 ± 0.026	0.419TrpLys: 0.419 ± 0.02	1.647TrpLeu: 1.647 ± 0.047	0.383TrpMet: 0.383 ± 0.02	0.418TrpAsn: 0.418 ± 0.02	0.739TrpPro: 0.739 ± 0.026	0.515TrpGln: 0.515 ± 0.022	1.116TrpArg: 1.116 ± 0.032	0.895TrpSer: 0.895 ± 0.026	0.869TrpThr: 0.869 ± 0.028	0.957TrpVal: 0.957 ± 0.027	0.243TrpTrp: 0.243 ± 0.016	0.28TrpTyr: 0.28 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.418TyrAla: 2.418 ± 0.045	0.213TyrCys: 0.213 ± 0.014	1.555TyrAsp: 1.555 ± 0.041	1.284TyrGlu: 1.284 ± 0.038	0.915TyrPhe: 0.915 ± 0.027	2.01TyrGly: 2.01 ± 0.045	0.505TyrHis: 0.505 ± 0.022	0.866TyrIle: 0.866 ± 0.026	0.526TyrLys: 0.526 ± 0.025	2.194TyrLeu: 2.194 ± 0.046	0.459TyrMet: 0.459 ± 0.024	0.523TyrAsn: 0.523 ± 0.021	1.018TyrPro: 1.018 ± 0.034	0.645TyrGln: 0.645 ± 0.025	1.649TyrArg: 1.649 ± 0.039	1.177TyrSer: 1.177 ± 0.033	1.034TyrThr: 1.034 ± 0.031	1.495TyrVal: 1.495 ± 0.042	0.359TyrTrp: 0.359 ± 0.018	0.531TyrTyr: 0.531 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3643 proteins (1110986 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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