Stigmatella aurantiaca (strain DW4/3-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Cystobacterineae; Archangiaceae; Stigmatella; Stigmatella aurantiaca

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8307 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q098Q7|Q098Q7_STIAD Alpha-D-phosphohexomutase OS=Stigmatella aurantiaca (strain DW4/3-1) OX=378806 GN=STAUR_6378 PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84FLRR5 pKa = 11.84SFWVLAALALTACSLLVDD23 pKa = 5.25FDD25 pKa = 6.11AEE27 pKa = 4.27GQPCDD32 pKa = 4.49SQNQCLAGYY41 pKa = 8.81VCEE44 pKa = 4.7NGSCVSGEE52 pKa = 4.15APPGGDD58 pKa = 3.52GGLGGPDD65 pKa = 3.02GGAGADD71 pKa = 3.61AGPGAGRR78 pKa = 11.84EE79 pKa = 4.16DD80 pKa = 3.92AGPDD84 pKa = 3.15AGADD88 pKa = 3.53GGDD91 pKa = 3.6GGRR94 pKa = 4.12

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3FWM3|E3FWM3_STIAD Uncharacterized protein OS=Stigmatella aurantiaca (strain DW4/3-1) OX=378806 GN=STAUR_0732 PE=4 SV=1
MM1 pKa = 7.64PWHH4 pKa = 6.72EE5 pKa = 4.57PVPHH9 pKa = 7.3RR10 pKa = 11.84PRR12 pKa = 11.84QRR14 pKa = 11.84AAPAFSLPGKK24 pKa = 9.16RR25 pKa = 11.84ACGTLRR31 pKa = 11.84PTIRR35 pKa = 11.84LLFSILFWSFFALSSLVFYY54 pKa = 11.03LGALVLWALTLPFDD68 pKa = 4.05RR69 pKa = 11.84NGRR72 pKa = 11.84ILHH75 pKa = 6.84LYY77 pKa = 8.69SCFWAQLYY85 pKa = 10.31IYY87 pKa = 9.82VNPLWSSRR95 pKa = 11.84VEE97 pKa = 4.07GRR99 pKa = 11.84EE100 pKa = 3.69NLPWRR105 pKa = 11.84GAAVLVANHH114 pKa = 6.58EE115 pKa = 4.46SLGDD119 pKa = 3.57ILVLFGLYY127 pKa = 10.06RR128 pKa = 11.84PFKK131 pKa = 9.35WVSKK135 pKa = 9.85AANFRR140 pKa = 11.84LPFIGWNMTLNRR152 pKa = 11.84YY153 pKa = 8.95VPLVRR158 pKa = 11.84GDD160 pKa = 3.64RR161 pKa = 11.84EE162 pKa = 4.35SVGRR166 pKa = 11.84MMVEE170 pKa = 3.85CEE172 pKa = 3.36KK173 pKa = 10.43WLLRR177 pKa = 11.84GVPILMFPEE186 pKa = 5.23GTRR189 pKa = 11.84SPDD192 pKa = 3.21GNIKK196 pKa = 10.35AFKK199 pKa = 10.66DD200 pKa = 3.68GAFQLAVKK208 pKa = 8.0LQCPVIPVVLTGTARR223 pKa = 11.84TLPKK227 pKa = 10.29HH228 pKa = 5.06GLKK231 pKa = 10.56LQTTARR237 pKa = 11.84CRR239 pKa = 11.84VRR241 pKa = 11.84VLPPVNPAEE250 pKa = 4.79HH251 pKa = 6.51GNSVPALLEE260 pKa = 3.84HH261 pKa = 6.24VRR263 pKa = 11.84NLMIEE268 pKa = 4.11EE269 pKa = 4.25KK270 pKa = 10.79ARR272 pKa = 11.84LEE274 pKa = 3.94AALAARR280 pKa = 11.84RR281 pKa = 3.75

Molecular weight: 31.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8307

0

8307

3100399

38

6261

373.2

40.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.601 ± 0.043

0.993 ± 0.012

4.7 ± 0.021

6.522 ± 0.034

3.408 ± 0.014

8.706 ± 0.03

2.164 ± 0.013

3.206 ± 0.018

3.055 ± 0.025

11.105 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.916 ± 0.011

2.324 ± 0.022

6.249 ± 0.025

3.753 ± 0.016

7.664 ± 0.035

6.013 ± 0.024

5.408 ± 0.04

7.633 ± 0.023

1.405 ± 0.012

2.175 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski