Actinomyces sp. oral taxon 448 str. F0400

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; unclassified Actinomyces; Actinomyces sp. oral taxon 448

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2518 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9EFJ4|F9EFJ4_9ACTO Ribose 5-phosphate isomerase OS=Actinomyces sp. oral taxon 448 str. F0400 OX=888056 GN=rpiB PE=3 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84LGRR5 pKa = 11.84SAATLGATAALVAAGAAVSTPAFANGDD32 pKa = 3.48FTYY35 pKa = 10.38TSDD38 pKa = 3.7YY39 pKa = 10.57TGVASPDD46 pKa = 3.21EE47 pKa = 4.23TLTISGTGCASTDD60 pKa = 3.48GNPTSVVWSVAADD73 pKa = 3.69QEE75 pKa = 4.84SLSSGASQATGAFGSIIPNDD95 pKa = 3.98DD96 pKa = 3.81GTWTAAIDD104 pKa = 3.91LPTATEE110 pKa = 3.82AAGLSDD116 pKa = 3.41QQRR119 pKa = 11.84GVLGFACYY127 pKa = 10.41NYY129 pKa = 10.38DD130 pKa = 3.58NKK132 pKa = 10.26VVDD135 pKa = 4.03SFNVSLALDD144 pKa = 3.55STKK147 pKa = 10.53PDD149 pKa = 2.85GSYY152 pKa = 9.62TLTTTADD159 pKa = 3.38GTQTIEE165 pKa = 3.86TTITGFTPEE174 pKa = 3.62EE175 pKa = 4.11TIRR178 pKa = 11.84FGVVPADD185 pKa = 3.41SHH187 pKa = 6.08GTGTSPTGDD196 pKa = 4.06DD197 pKa = 3.65FTEE200 pKa = 4.34LTTFTADD207 pKa = 3.27ADD209 pKa = 4.04GGATGSFPVPADD221 pKa = 3.83LPDD224 pKa = 2.83GRR226 pKa = 11.84YY227 pKa = 8.93RR228 pKa = 11.84VGVVGDD234 pKa = 3.26SHH236 pKa = 8.17GEE238 pKa = 3.56YY239 pKa = 10.71GYY241 pKa = 10.49FQNLLVKK248 pKa = 10.54DD249 pKa = 3.78GTYY252 pKa = 10.55SITSEE257 pKa = 4.38DD258 pKa = 3.66GSTTGTGSSDD268 pKa = 3.19SSSSTDD274 pKa = 2.83

Molecular weight: 27.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9ED42|F9ED42_9ACTO DNA replication and repair protein RecF OS=Actinomyces sp. oral taxon 448 str. F0400 OX=888056 GN=recF PE=3 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.4VHH17 pKa = 5.52GFRR20 pKa = 11.84KK21 pKa = 9.95RR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AVLASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.61GRR40 pKa = 11.84ARR42 pKa = 11.84LAAA45 pKa = 4.44

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2518

0

2518

817327

37

3188

324.6

34.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.681 ± 0.073

0.809 ± 0.014

6.271 ± 0.048

5.434 ± 0.051

2.436 ± 0.027

9.301 ± 0.051

2.001 ± 0.024

3.885 ± 0.035

1.578 ± 0.031

9.85 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.018

1.766 ± 0.024

5.764 ± 0.046

2.503 ± 0.024

8.239 ± 0.06

6.023 ± 0.043

6.331 ± 0.04

8.753 ± 0.052

1.454 ± 0.021

1.891 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski