Sclerotinia sclerotiorum megabirnavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Megabirnaviridae; Megabirnavirus; unclassified Megabirnavirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G3FL62|A0A0G3FL62_9VIRU Uncharacterized protein OS=Sclerotinia sclerotiorum megabirnavirus 1 OX=1661257 PE=4 SV=1
MM1 pKa = 7.51LANATALGEE10 pKa = 4.03QMLVIGDD17 pKa = 3.69EE18 pKa = 4.44LGRR21 pKa = 11.84EE22 pKa = 4.2SGDD25 pKa = 3.21AEE27 pKa = 4.03PRR29 pKa = 11.84GKK31 pKa = 9.11RR32 pKa = 11.84TLRR35 pKa = 11.84DD36 pKa = 3.45FPMLEE41 pKa = 4.07FSNAVHH47 pKa = 6.79AVMADD52 pKa = 3.47VEE54 pKa = 4.54LTSWDD59 pKa = 3.89AVCDD63 pKa = 3.57QHH65 pKa = 6.96TKK67 pKa = 8.68NTPQTLLVDD76 pKa = 4.09SPLLFSMAGQLLADD90 pKa = 4.28DD91 pKa = 5.06FARR94 pKa = 11.84AGVMIEE100 pKa = 3.78GRR102 pKa = 11.84QFLLLGRR109 pKa = 11.84NGHH112 pKa = 5.43TPVRR116 pKa = 11.84MDD118 pKa = 3.02TGTYY122 pKa = 10.81ASIKK126 pKa = 9.58TSLLRR131 pKa = 11.84HH132 pKa = 5.95ASPRR136 pKa = 11.84RR137 pKa = 11.84LRR139 pKa = 11.84AARR142 pKa = 11.84RR143 pKa = 11.84LFEE146 pKa = 4.84NVFAMNVPDD155 pKa = 4.99FLAVLEE161 pKa = 4.59AQHH164 pKa = 7.01DD165 pKa = 4.38CGPEE169 pKa = 3.62MDD171 pKa = 4.79SFFSMFSGDD180 pKa = 3.22GRR182 pKa = 11.84SLVVEE187 pKa = 4.75GAPTPCVGKK196 pKa = 9.94FLYY199 pKa = 10.03KK200 pKa = 10.47QLDD203 pKa = 3.48GGEE206 pKa = 4.16WVATDD211 pKa = 3.72VSAKK215 pKa = 8.76TASEE219 pKa = 3.92ALRR222 pKa = 11.84NAMVLRR228 pKa = 11.84KK229 pKa = 8.68LHH231 pKa = 7.04CDD233 pKa = 3.24GEE235 pKa = 4.65MGWEE239 pKa = 3.91QVLGLVEE246 pKa = 4.47RR247 pKa = 11.84SLISRR252 pKa = 11.84DD253 pKa = 3.11VHH255 pKa = 7.06LLSDD259 pKa = 4.78CIAYY263 pKa = 9.08LFPYY267 pKa = 8.31TEE269 pKa = 3.97RR270 pKa = 11.84SLYY273 pKa = 9.4QRR275 pKa = 11.84SRR277 pKa = 11.84MTFPLLSRR285 pKa = 11.84IILRR289 pKa = 11.84SIDD292 pKa = 3.87LGHH295 pKa = 5.76STEE298 pKa = 4.4GDD300 pKa = 3.07ILSLMRR306 pKa = 11.84HH307 pKa = 5.08MSGLGHH313 pKa = 5.81QASSAFFLYY322 pKa = 10.68SLWLPDD328 pKa = 3.27GAGRR332 pKa = 11.84HH333 pKa = 4.83VVDD336 pKa = 4.71FFCRR340 pKa = 11.84KK341 pKa = 7.89GWFNGGYY348 pKa = 9.06YY349 pKa = 10.41AVEE352 pKa = 3.97KK353 pKa = 10.33RR354 pKa = 11.84LKK356 pKa = 10.2ALHH359 pKa = 5.99SLARR363 pKa = 11.84RR364 pKa = 11.84CRR366 pKa = 11.84YY367 pKa = 9.04VPKK370 pKa = 10.19EE371 pKa = 3.47LRR373 pKa = 11.84GYY375 pKa = 8.9NVRR378 pKa = 11.84PTDD381 pKa = 3.74LMYY384 pKa = 9.99LTSVLGRR391 pKa = 11.84FYY393 pKa = 11.37LDD395 pKa = 4.11FAADD399 pKa = 3.84DD400 pKa = 4.09SAVSDD405 pKa = 3.26RR406 pKa = 11.84TEE408 pKa = 3.84YY409 pKa = 10.85YY410 pKa = 10.66GEE412 pKa = 4.1HH413 pKa = 6.64LSYY416 pKa = 11.31DD417 pKa = 3.87GVLGIYY423 pKa = 8.9STDD426 pKa = 2.94AHH428 pKa = 6.19KK429 pKa = 10.89QGMMRR434 pKa = 11.84FLLDD438 pKa = 3.55RR439 pKa = 11.84SRR441 pKa = 11.84VAGRR445 pKa = 11.84LWQQSEE451 pKa = 4.48VSSLGDD457 pKa = 2.74WMMRR461 pKa = 11.84YY462 pKa = 9.87VMYY465 pKa = 10.51GSSGSAGGHH474 pKa = 5.98SGDD477 pKa = 4.48EE478 pKa = 4.38FNVDD482 pKa = 2.95HH483 pKa = 6.69SVSKK487 pKa = 10.74RR488 pKa = 11.84LWLSNRR494 pKa = 11.84DD495 pKa = 3.06EE496 pKa = 5.24DD497 pKa = 3.93YY498 pKa = 11.23AAAYY502 pKa = 10.0VYY504 pKa = 9.83EE505 pKa = 4.51EE506 pKa = 4.12PAIASSVTVVKK517 pKa = 10.72RR518 pKa = 11.84EE519 pKa = 4.02AGKK522 pKa = 10.21LRR524 pKa = 11.84QLLPARR530 pKa = 11.84IPHH533 pKa = 6.93WLTEE537 pKa = 4.05SLLLSEE543 pKa = 4.85IEE545 pKa = 4.23SGILRR550 pKa = 11.84SMPLSLEE557 pKa = 3.9MSADD561 pKa = 3.29KK562 pKa = 11.28AMEE565 pKa = 4.33GVLQRR570 pKa = 11.84RR571 pKa = 11.84KK572 pKa = 10.96AMMSGEE578 pKa = 4.24TVACVDD584 pKa = 3.15WADD587 pKa = 3.9FNITHH592 pKa = 6.9TLSDD596 pKa = 3.23MAAYY600 pKa = 10.12FRR602 pKa = 11.84MLGKK606 pKa = 9.76QALEE610 pKa = 4.24TCTEE614 pKa = 3.87PNYY617 pKa = 10.48WNGISKK623 pKa = 10.11GQFLHH628 pKa = 7.44DD629 pKa = 4.03VAEE632 pKa = 4.71HH633 pKa = 6.66CATTLYY639 pKa = 10.85NLWIRR644 pKa = 11.84NGATADD650 pKa = 3.39SHH652 pKa = 7.16FEE654 pKa = 3.84HH655 pKa = 7.18AVRR658 pKa = 11.84GLWSGWRR665 pKa = 11.84STQFFNSSFNVAYY678 pKa = 10.8CEE680 pKa = 4.29TNFDD684 pKa = 3.43AMHH687 pKa = 7.24ALFQSPLPNRR697 pKa = 11.84ADD699 pKa = 3.36HH700 pKa = 7.13AGDD703 pKa = 4.58DD704 pKa = 4.4FFGTYY709 pKa = 10.26NDD711 pKa = 4.66PLDD714 pKa = 3.51AMRR717 pKa = 11.84FVLSMPLAQHH727 pKa = 6.67DD728 pKa = 4.45VNPTRR733 pKa = 11.84QLVDD737 pKa = 3.16GDD739 pKa = 3.49VGEE742 pKa = 4.47FLRR745 pKa = 11.84NEE747 pKa = 3.99YY748 pKa = 9.68MRR750 pKa = 11.84DD751 pKa = 3.86GIVHH755 pKa = 6.93GSFQRR760 pKa = 11.84SIGSFVGSDD769 pKa = 3.46LQSPEE774 pKa = 4.35LFSSITQAQGSNVRR788 pKa = 11.84RR789 pKa = 11.84GADD792 pKa = 3.05LSVIEE797 pKa = 4.58EE798 pKa = 3.87YY799 pKa = 10.38RR800 pKa = 11.84YY801 pKa = 8.81TIVQYY806 pKa = 9.73WATVYY811 pKa = 10.79SPEE814 pKa = 4.57SKK816 pKa = 9.72TSATPSVDD824 pKa = 3.54LCQLDD829 pKa = 4.25PTCGGLGCPRR839 pKa = 11.84YY840 pKa = 9.48GQQSTIATGSINTMPVQRR858 pKa = 11.84RR859 pKa = 11.84VPLDD863 pKa = 3.27VIMPRR868 pKa = 11.84NAAVLLQRR876 pKa = 11.84FKK878 pKa = 11.23QRR880 pKa = 11.84LWSYY884 pKa = 11.2NITSTNLEE892 pKa = 4.06GLRR895 pKa = 11.84DD896 pKa = 3.9DD897 pKa = 5.54VMIATYY903 pKa = 10.6GSDD906 pKa = 3.59FPAEE910 pKa = 3.73IKK912 pKa = 10.44KK913 pKa = 10.71RR914 pKa = 11.84LDD916 pKa = 3.13RR917 pKa = 11.84EE918 pKa = 4.05LRR920 pKa = 11.84EE921 pKa = 4.24TQVQWILEE929 pKa = 4.16ANKK932 pKa = 9.85RR933 pKa = 11.84VAVMRR938 pKa = 11.84KK939 pKa = 8.77AVLPAVPWWVDD950 pKa = 3.35DD951 pKa = 4.4YY952 pKa = 11.53IQTTIQRR959 pKa = 11.84VLANEE964 pKa = 4.0EE965 pKa = 4.15LDD967 pKa = 4.44GNSADD972 pKa = 4.13LNSMAMVVRR981 pKa = 11.84SMALAEE987 pKa = 4.21FSPLDD992 pKa = 3.39SALSHH997 pKa = 6.32LQTTAAQITGLPAIVKK1013 pKa = 9.77LSRR1016 pKa = 11.84GRR1018 pKa = 11.84VNPILSRR1025 pKa = 11.84MRR1027 pKa = 11.84VYY1029 pKa = 11.01LSDD1032 pKa = 3.76PMVEE1036 pKa = 4.0ALVEE1040 pKa = 4.27HH1041 pKa = 7.02RR1042 pKa = 11.84WNLPTSTGGVIPSEE1056 pKa = 4.08LRR1058 pKa = 11.84AVIYY1062 pKa = 10.68NVLNYY1067 pKa = 10.83ALYY1070 pKa = 8.81QVRR1073 pKa = 11.84YY1074 pKa = 8.73INQSFEE1080 pKa = 3.89SDD1082 pKa = 3.54ARR1084 pKa = 11.84WLTMYY1089 pKa = 8.34ATSVCQRR1096 pKa = 11.84LSNSWRR1102 pKa = 11.84QKK1104 pKa = 9.25CGTMFEE1110 pKa = 4.27IQDD1113 pKa = 4.13EE1114 pKa = 4.25ISYY1117 pKa = 11.14

Molecular weight: 125.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G3FL62|A0A0G3FL62_9VIRU Uncharacterized protein OS=Sclerotinia sclerotiorum megabirnavirus 1 OX=1661257 PE=4 SV=1
MM1 pKa = 7.31VKK3 pKa = 10.29RR4 pKa = 11.84EE5 pKa = 4.26GFRR8 pKa = 11.84ITYY11 pKa = 7.63VWRR14 pKa = 11.84TYY16 pKa = 10.68EE17 pKa = 3.93YY18 pKa = 11.31GRR20 pKa = 11.84LALLYY25 pKa = 9.93RR26 pKa = 11.84PQDD29 pKa = 3.03RR30 pKa = 11.84GRR32 pKa = 11.84SVLAQLIVRR41 pKa = 11.84NGPALLRR48 pKa = 11.84DD49 pKa = 3.83MRR51 pKa = 11.84SGTTRR56 pKa = 11.84NSLRR60 pKa = 11.84VMVCISALVSLLARR74 pKa = 11.84ARR76 pKa = 11.84TTLVKK81 pKa = 10.65VKK83 pKa = 10.06QAEE86 pKa = 4.18LASAEE91 pKa = 3.89QRR93 pKa = 11.84DD94 pKa = 3.85RR95 pKa = 11.84EE96 pKa = 3.72RR97 pKa = 11.84SYY99 pKa = 9.87ILRR102 pKa = 11.84RR103 pKa = 11.84NGHH106 pKa = 5.64CRR108 pKa = 11.84AVIRR112 pKa = 11.84YY113 pKa = 8.7AIARR117 pKa = 11.84RR118 pKa = 11.84DD119 pKa = 3.57EE120 pKa = 4.1QVRR123 pKa = 11.84TEE125 pKa = 4.01AVRR128 pKa = 11.84AEE130 pKa = 4.31TFAEE134 pKa = 3.76QRR136 pKa = 11.84LISEE140 pKa = 4.6HH141 pKa = 5.88SAIVDD146 pKa = 3.97LVAQLLAEE154 pKa = 4.08EE155 pKa = 4.83AEE157 pKa = 4.54PLFEE161 pKa = 3.88AHH163 pKa = 6.97KK164 pKa = 10.11AAEE167 pKa = 4.11VSPTLVRR174 pKa = 11.84EE175 pKa = 4.16DD176 pKa = 3.51QAPEE180 pKa = 3.84NVPEE184 pKa = 4.13EE185 pKa = 4.17EE186 pKa = 4.57GEE188 pKa = 3.87ISDD191 pKa = 4.35FVFGVAAVWRR201 pKa = 11.84AINIRR206 pKa = 11.84AWILRR211 pKa = 11.84GQYY214 pKa = 10.32NYY216 pKa = 8.49RR217 pKa = 11.84TRR219 pKa = 11.84KK220 pKa = 9.22LAQQRR225 pKa = 11.84LAEE228 pKa = 4.34MNDD231 pKa = 2.99VAARR235 pKa = 11.84ASDD238 pKa = 3.92DD239 pKa = 3.66VGDD242 pKa = 3.78TSGRR246 pKa = 11.84QPSEE250 pKa = 3.34AATFGHH256 pKa = 6.69HH257 pKa = 6.35FSCFPALGICNCDD270 pKa = 2.99AHH272 pKa = 6.88AVSAGAPAVEE282 pKa = 4.3VMTDD286 pKa = 3.2EE287 pKa = 4.42QVCKK291 pKa = 10.22KK292 pKa = 10.69CEE294 pKa = 4.04IITAQVFEE302 pKa = 4.7RR303 pKa = 11.84NGRR306 pKa = 11.84ANVSIKK312 pKa = 10.33VVPPMFARR320 pKa = 11.84AAFEE324 pKa = 4.71ADD326 pKa = 3.55GSDD329 pKa = 3.54LRR331 pKa = 11.84IWNIAVRR338 pKa = 11.84RR339 pKa = 11.84AGYY342 pKa = 10.27EE343 pKa = 3.61EE344 pKa = 6.13LIPKK348 pKa = 8.55MRR350 pKa = 11.84TILANSTGG358 pKa = 3.37

Molecular weight: 40.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4352

358

1626

1088.0

120.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.754 ± 0.862

1.425 ± 0.243

6.089 ± 0.284

5.193 ± 0.369

3.47 ± 0.376

7.652 ± 0.472

2.528 ± 0.115

3.608 ± 0.211

2.459 ± 0.072

9.421 ± 0.754

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.056 ± 0.413

4.435 ± 0.611

3.814 ± 0.154

3.814 ± 0.029

7.79 ± 0.544

6.434 ± 0.81

5.79 ± 0.317

7.399 ± 0.357

1.769 ± 0.132

3.102 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski