Chitinophaga lutea
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5334 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4PAA0|A0A3N4PAA0_9BACT Uncharacterized protein OS=Chitinophaga lutea OX=2488634 GN=EGT74_24635 PE=4 SV=1
MM1 pKa = 7.59 FNITVDD7 pKa = 3.55 GYY9 pKa = 9.37 EE10 pKa = 3.85 YY11 pKa = 10.55 RR12 pKa = 11.84 VTEE15 pKa = 4.04 IVDD18 pKa = 3.8 DD19 pKa = 4.38 LSSTFAVEE27 pKa = 4.08 IEE29 pKa = 3.98 GDD31 pKa = 3.69 PVVFKK36 pKa = 11.09 VDD38 pKa = 3.52 EE39 pKa = 4.27 EE40 pKa = 4.81 SISLQAVDD48 pKa = 4.0 HH49 pKa = 6.74 EE50 pKa = 4.74 YY51 pKa = 11.23 GIDD54 pKa = 3.27 HH55 pKa = 6.82 HH56 pKa = 7.3 LDD58 pKa = 2.9 RR59 pKa = 11.84 LYY61 pKa = 11.24 AQIASAIEE69 pKa = 4.66 DD70 pKa = 3.82 YY71 pKa = 11.37 LEE73 pKa = 4.16 SLL75 pKa = 4.22
Molecular weight: 8.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.884
IPC_protein 3.821
Toseland 3.63
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.541
Solomon 3.783
Lehninger 3.745
Nozaki 3.935
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.935
Patrickios 0.846
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.845
Protein with the highest isoelectric point:
>tr|A0A3N4PZ44|A0A3N4PZ44_9BACT Uncharacterized protein OS=Chitinophaga lutea OX=2488634 GN=EGT74_18260 PE=4 SV=1
MM1 pKa = 7.37 LPSGKK6 pKa = 8.84 MFQGKK11 pKa = 9.81 KK12 pKa = 9.67 ISQKK16 pKa = 9.18 KK17 pKa = 9.65 CCMQTFSLKK26 pKa = 10.42 SHH28 pKa = 6.49 LPDD31 pKa = 4.17 RR32 pKa = 11.84 AAQPSAFFVQSRR44 pKa = 11.84 GRR46 pKa = 11.84 NTGRR50 pKa = 11.84 PSYY53 pKa = 10.68 APRR56 pKa = 11.84 RR57 pKa = 11.84 NCYY60 pKa = 10.23 VIACQEE66 pKa = 3.7 QDD68 pKa = 3.35 LSVTYY73 pKa = 9.83 WLVYY77 pKa = 10.48 ALWKK81 pKa = 10.21 SGHH84 pKa = 5.5 FRR86 pKa = 11.84 RR87 pKa = 11.84 NLQGTRR93 pKa = 11.84 TPYY96 pKa = 10.17 IRR98 pKa = 11.84 VGHH101 pKa = 5.53 MRR103 pKa = 11.84 HH104 pKa = 6.22 LIARR108 pKa = 11.84 AVRR111 pKa = 11.84 RR112 pKa = 11.84 YY113 pKa = 9.22 AHH115 pKa = 6.38 EE116 pKa = 4.51 LEE118 pKa = 5.07 PVQQLQKK125 pKa = 9.51 TLQSEE130 pKa = 4.06 MRR132 pKa = 11.84 LIRR135 pKa = 11.84 EE136 pKa = 4.44 LDD138 pKa = 3.0 RR139 pKa = 11.84 VARR142 pKa = 11.84 MRR144 pKa = 11.84 TVLLADD150 pKa = 5.72 LIDD153 pKa = 5.05 HH154 pKa = 7.14 IAA156 pKa = 3.51
Molecular weight: 18.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.838
IPC_protein 10.76
Toseland 10.76
ProMoST 10.555
Dawson 10.862
Bjellqvist 10.628
Wikipedia 11.111
Rodwell 11.008
Grimsley 10.921
Solomon 11.008
Lehninger 10.965
Nozaki 10.76
DTASelect 10.613
Thurlkill 10.774
EMBOSS 11.169
Sillero 10.804
Patrickios 10.716
IPC_peptide 11.008
IPC2_peptide 9.838
IPC2.peptide.svr19 8.393
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5334
0
5334
1965502
26
8072
368.5
41.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.799 ± 0.043
0.82 ± 0.013
5.089 ± 0.02
5.5 ± 0.035
4.634 ± 0.025
7.46 ± 0.031
2.031 ± 0.018
6.026 ± 0.028
5.83 ± 0.039
9.446 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.462 ± 0.017
4.871 ± 0.036
4.409 ± 0.022
3.982 ± 0.022
5.044 ± 0.026
5.719 ± 0.023
5.867 ± 0.051
6.659 ± 0.028
1.355 ± 0.015
3.996 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here