Streptococcus satellite phage Javan404

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZM44|A0A4D5ZM44_9VIRU Cadmium resistance protein OS=Streptococcus satellite phage Javan404 OX=2558683 GN=JavanS404_0003 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.4NLEE5 pKa = 4.17EE6 pKa = 5.44KK7 pKa = 10.32IEE9 pKa = 3.99PVVIIIDD16 pKa = 3.8EE17 pKa = 4.17EE18 pKa = 4.64DD19 pKa = 3.74DD20 pKa = 3.93FDD22 pKa = 5.94FEE24 pKa = 4.36EE25 pKa = 4.73TDD27 pKa = 5.58DD28 pKa = 4.88EE29 pKa = 4.43IQAQYY34 pKa = 10.54EE35 pKa = 4.39AEE37 pKa = 4.18CLEE40 pKa = 5.78DD41 pKa = 3.71DD42 pKa = 4.11TQDD45 pKa = 3.43VINWINEE52 pKa = 4.21SNTLTEE58 pKa = 4.22YY59 pKa = 11.41VSMEE63 pKa = 4.41FEE65 pKa = 5.29NMNQDD70 pKa = 3.68DD71 pKa = 4.68LGASLEE77 pKa = 4.2NASQCLDD84 pKa = 4.31AIVGMLEE91 pKa = 3.76NGTLVLAGGKK101 pKa = 9.33HH102 pKa = 5.1EE103 pKa = 4.14NN104 pKa = 3.61

Molecular weight: 11.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZMK1|A0A4D5ZMK1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan404 OX=2558683 GN=JavanS404_0009 PE=4 SV=1
MM1 pKa = 7.92KK2 pKa = 9.26ITEE5 pKa = 4.13YY6 pKa = 10.85KK7 pKa = 9.78KK8 pKa = 10.4NNGATVYY15 pKa = 10.32RR16 pKa = 11.84SQVYY20 pKa = 10.41LGVDD24 pKa = 3.27VVTGKK29 pKa = 10.49KK30 pKa = 9.74IKK32 pKa = 9.46TSVSARR38 pKa = 11.84TKK40 pKa = 10.94KK41 pKa = 9.63EE42 pKa = 3.83VKK44 pKa = 10.37LLATQKK50 pKa = 10.89KK51 pKa = 10.17YY52 pKa = 11.07DD53 pKa = 3.74FKK55 pKa = 11.47QNGSTTYY62 pKa = 10.28KK63 pKa = 10.1SVSIEE68 pKa = 4.17TFDD71 pKa = 4.08EE72 pKa = 4.6LTNLWLEE79 pKa = 4.4SYY81 pKa = 10.64KK82 pKa = 10.4LTVKK86 pKa = 9.78PQSYY90 pKa = 10.65KK91 pKa = 9.82NTISKK96 pKa = 9.52IDD98 pKa = 3.53CHH100 pKa = 5.91IRR102 pKa = 11.84PYY104 pKa = 9.88FGHH107 pKa = 6.23MKK109 pKa = 10.1LNKK112 pKa = 8.38ITSSTIQVFINDD124 pKa = 3.08ISKK127 pKa = 8.46TQGTYY132 pKa = 10.46VMMRR136 pKa = 11.84SIIKK140 pKa = 10.33RR141 pKa = 11.84ILQQGVLLNLIPSNPARR158 pKa = 11.84DD159 pKa = 3.39IILPRR164 pKa = 11.84KK165 pKa = 9.44QKK167 pKa = 9.71TEE169 pKa = 3.71NNKK172 pKa = 9.44VKK174 pKa = 10.56FIEE177 pKa = 4.68KK178 pKa = 10.08EE179 pKa = 3.95DD180 pKa = 3.55LKK182 pKa = 11.3KK183 pKa = 10.83FLDD186 pKa = 4.5HH187 pKa = 7.13IEE189 pKa = 4.09KK190 pKa = 10.39KK191 pKa = 10.56SYY193 pKa = 10.14GRR195 pKa = 11.84YY196 pKa = 9.64GLYY199 pKa = 10.09FDD201 pKa = 4.29YY202 pKa = 11.22VLYY205 pKa = 11.02NLLLATGLRR214 pKa = 11.84VGEE217 pKa = 4.2ACALEE222 pKa = 4.28WSDD225 pKa = 4.33IDD227 pKa = 4.56LEE229 pKa = 4.33NGIITVTKK237 pKa = 9.74TYY239 pKa = 10.89NKK241 pKa = 9.33SLKK244 pKa = 10.16IISTTKK250 pKa = 8.68TKK252 pKa = 10.38SGNRR256 pKa = 11.84IISIGQDD263 pKa = 2.98TVNLMKK269 pKa = 10.46LYY271 pKa = 10.44KK272 pKa = 10.15ARR274 pKa = 11.84QRR276 pKa = 11.84QLFSEE281 pKa = 4.65ISSNAPRR288 pKa = 11.84IVFSTPTRR296 pKa = 11.84DD297 pKa = 3.56YY298 pKa = 10.74FDD300 pKa = 3.4NATRR304 pKa = 11.84QCALDD309 pKa = 3.98TMCGQAGVPRR319 pKa = 11.84FTFHH323 pKa = 7.91AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.51RR346 pKa = 11.84LGHH349 pKa = 6.41ANISMTLDD357 pKa = 3.31IYY359 pKa = 11.6SHH361 pKa = 6.83LSKK364 pKa = 10.92DD365 pKa = 3.51KK366 pKa = 10.41EE367 pKa = 4.26KK368 pKa = 10.88EE369 pKa = 3.87AVSYY373 pKa = 10.09FEE375 pKa = 4.84KK376 pKa = 10.98AMNGLL381 pKa = 3.71

Molecular weight: 43.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

2977

48

519

148.8

17.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.173 ± 0.711

0.773 ± 0.151

6.382 ± 0.599

8.767 ± 0.938

4.4 ± 0.456

4.232 ± 0.496

1.176 ± 0.175

8.666 ± 0.417

9.943 ± 0.775

10.312 ± 0.698

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.226

5.979 ± 0.518

2.049 ± 0.24

4.434 ± 0.399

3.661 ± 0.391

5.912 ± 0.604

5.173 ± 0.481

5.24 ± 0.404

0.705 ± 0.137

4.501 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski