Brevundimonas sp. S30B

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas; unclassified Brevundimonas

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9RK51|A0A4Y9RK51_9CAUL S9 family peptidase OS=Brevundimonas sp. S30B OX=2561925 GN=E4M02_05460 PE=4 SV=1
MM1 pKa = 7.94SDD3 pKa = 2.94TDD5 pKa = 3.39IDD7 pKa = 4.26DD8 pKa = 4.72RR9 pKa = 11.84GLTPEE14 pKa = 5.3DD15 pKa = 3.68PADD18 pKa = 4.08PAAHH22 pKa = 5.22QHH24 pKa = 6.76PDD26 pKa = 3.08PTLCPEE32 pKa = 4.27CQGVGALPSGEE43 pKa = 4.16TCPVCEE49 pKa = 4.53GSGKK53 pKa = 10.49AVGRR57 pKa = 11.84TGGGG61 pKa = 3.05

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9R926|A0A4Y9R926_9CAUL Probable nicotinate-nucleotide adenylyltransferase OS=Brevundimonas sp. S30B OX=2561925 GN=nadD PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.2NGQKK29 pKa = 9.53IVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2779

0

2779

846945

24

1617

304.8

32.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.944 ± 0.071

0.706 ± 0.012

6.085 ± 0.043

5.74 ± 0.046

3.407 ± 0.033

9.053 ± 0.044

1.759 ± 0.022

4.282 ± 0.031

2.61 ± 0.042

9.935 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.022

2.267 ± 0.031

5.482 ± 0.039

3.279 ± 0.03

7.913 ± 0.048

5.067 ± 0.033

5.187 ± 0.035

7.557 ± 0.033

1.465 ± 0.019

1.993 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski