Amino acid dipepetide frequency for Brevundimonas sp. S30B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.69AlaAla: 20.69 ± 0.258	1.2AlaCys: 1.2 ± 0.039	8.206AlaAsp: 8.206 ± 0.104	8.93AlaGlu: 8.93 ± 0.121	4.866AlaPhe: 4.866 ± 0.079	12.117AlaGly: 12.117 ± 0.154	2.269AlaHis: 2.269 ± 0.055	5.134AlaIle: 5.134 ± 0.077	3.31AlaLys: 3.31 ± 0.068	14.969AlaLeu: 14.969 ± 0.165	3.457AlaMet: 3.457 ± 0.075	2.777AlaAsn: 2.777 ± 0.064	7.522AlaPro: 7.522 ± 0.137	4.783AlaGln: 4.783 ± 0.085	11.394AlaArg: 11.394 ± 0.149	6.815AlaSer: 6.815 ± 0.093	5.794AlaThr: 5.794 ± 0.09	10.348AlaVal: 10.348 ± 0.113	2.085AlaTrp: 2.085 ± 0.057	2.781AlaTyr: 2.781 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.985CysAla: 0.985 ± 0.04	0.086CysCys: 0.086 ± 0.011	0.515CysAsp: 0.515 ± 0.026	0.388CysGlu: 0.388 ± 0.02	0.242CysPhe: 0.242 ± 0.018	0.79CysGly: 0.79 ± 0.034	0.181CysHis: 0.181 ± 0.015	0.273CysIle: 0.273 ± 0.019	0.139CysLys: 0.139 ± 0.013	0.595CysLeu: 0.595 ± 0.027	0.103CysMet: 0.103 ± 0.011	0.156CysAsn: 0.156 ± 0.014	0.423CysPro: 0.423 ± 0.023	0.189CysGln: 0.189 ± 0.014	0.531CysArg: 0.531 ± 0.026	0.362CysSer: 0.362 ± 0.019	0.306CysThr: 0.306 ± 0.02	0.563CysVal: 0.563 ± 0.025	0.099CysTrp: 0.099 ± 0.011	0.132CysTyr: 0.132 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	8.291AspAla: 8.291 ± 0.105	0.418AspCys: 0.418 ± 0.023	3.549AspAsp: 3.549 ± 0.076	3.527AspGlu: 3.527 ± 0.076	2.086AspPhe: 2.086 ± 0.053	5.941AspGly: 5.941 ± 0.099	1.34AspHis: 1.34 ± 0.044	2.614AspIle: 2.614 ± 0.066	1.419AspLys: 1.419 ± 0.055	6.482AspLeu: 6.482 ± 0.107	1.292AspMet: 1.292 ± 0.039	1.171AspAsn: 1.171 ± 0.041	4.044AspPro: 4.044 ± 0.064	2.22AspGln: 2.22 ± 0.049	5.277AspArg: 5.277 ± 0.08	1.997AspSer: 1.997 ± 0.05	2.36AspThr: 2.36 ± 0.053	4.183AspVal: 4.183 ± 0.081	1.156AspTrp: 1.156 ± 0.036	1.484AspTyr: 1.484 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	9.63GluAla: 9.63 ± 0.119	0.251GluCys: 0.251 ± 0.017	3.016GluAsp: 3.016 ± 0.069	2.709GluGlu: 2.709 ± 0.06	1.482GluPhe: 1.482 ± 0.05	5.058GluGly: 5.058 ± 0.086	1.027GluHis: 1.027 ± 0.036	2.986GluIle: 2.986 ± 0.072	1.616GluLys: 1.616 ± 0.057	4.874GluLeu: 4.874 ± 0.077	1.351GluMet: 1.351 ± 0.041	1.207GluAsn: 1.207 ± 0.041	3.02GluPro: 3.02 ± 0.066	2.086GluGln: 2.086 ± 0.053	5.464GluArg: 5.464 ± 0.108	2.111GluSer: 2.111 ± 0.049	3.848GluThr: 3.848 ± 0.079	4.111GluVal: 4.111 ± 0.066	0.723GluTrp: 0.723 ± 0.029	0.828GluTyr: 0.828 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.471PheAla: 4.471 ± 0.086	0.322PheCys: 0.322 ± 0.02	2.765PheAsp: 2.765 ± 0.057	2.203PheGlu: 2.203 ± 0.049	1.171PhePhe: 1.171 ± 0.046	3.432PheGly: 3.432 ± 0.073	0.586PheHis: 0.586 ± 0.027	1.462PheIle: 1.462 ± 0.046	0.891PheLys: 0.891 ± 0.034	2.84PheLeu: 2.84 ± 0.063	0.71PheMet: 0.71 ± 0.029	0.979PheAsn: 0.979 ± 0.037	1.335PhePro: 1.335 ± 0.041	1.131PheGln: 1.131 ± 0.04	2.124PheArg: 2.124 ± 0.053	1.866PheSer: 1.866 ± 0.057	1.879PheThr: 1.879 ± 0.057	2.637PheVal: 2.637 ± 0.058	0.509PheTrp: 0.509 ± 0.027	0.753PheTyr: 0.753 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	11.304GlyAla: 11.304 ± 0.153	0.754GlyCys: 0.754 ± 0.029	5.255GlyAsp: 5.255 ± 0.093	5.307GlyGlu: 5.307 ± 0.089	3.573GlyPhe: 3.573 ± 0.07	8.754GlyGly: 8.754 ± 0.147	1.688GlyHis: 1.688 ± 0.05	2.654GlyIle: 2.654 ± 0.067	2.404GlyLys: 2.404 ± 0.06	10.01GlyLeu: 10.01 ± 0.12	2.062GlyMet: 2.062 ± 0.054	1.457GlyAsn: 1.457 ± 0.054	4.349GlyPro: 4.349 ± 0.07	3.494GlyGln: 3.494 ± 0.068	7.906GlyArg: 7.906 ± 0.111	4.573GlySer: 4.573 ± 0.086	3.189GlyThr: 3.189 ± 0.078	8.031GlyVal: 8.031 ± 0.099	1.661GlyTrp: 1.661 ± 0.045	2.103GlyTyr: 2.103 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	2.356HisAla: 2.356 ± 0.064	0.164HisCys: 0.164 ± 0.014	1.172HisAsp: 1.172 ± 0.038	0.988HisGlu: 0.988 ± 0.032	0.603HisPhe: 0.603 ± 0.029	1.788HisGly: 1.788 ± 0.053	0.42HisHis: 0.42 ± 0.026	0.661HisIle: 0.661 ± 0.032	0.375HisLys: 0.375 ± 0.022	1.756HisLeu: 1.756 ± 0.046	0.41HisMet: 0.41 ± 0.023	0.367HisAsn: 0.367 ± 0.019	1.203HisPro: 1.203 ± 0.045	0.561HisGln: 0.561 ± 0.024	1.37HisArg: 1.37 ± 0.04	0.693HisSer: 0.693 ± 0.03	0.697HisThr: 0.697 ± 0.031	1.304HisVal: 1.304 ± 0.039	0.282HisTrp: 0.282 ± 0.018	0.422HisTyr: 0.422 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.084IleAla: 6.084 ± 0.084	0.327IleCys: 0.327 ± 0.019	3.354IleAsp: 3.354 ± 0.059	3.256IleGlu: 3.256 ± 0.07	1.231IlePhe: 1.231 ± 0.047	4.306IleGly: 4.306 ± 0.082	0.777IleHis: 0.777 ± 0.03	1.612IleIle: 1.612 ± 0.052	1.069IleLys: 1.069 ± 0.041	3.746IleLeu: 3.746 ± 0.077	0.739IleMet: 0.739 ± 0.027	1.119IleAsn: 1.119 ± 0.04	1.814IlePro: 1.814 ± 0.045	1.306IleGln: 1.306 ± 0.04	2.892IleArg: 2.892 ± 0.062	2.128IleSer: 2.128 ± 0.052	2.2IleThr: 2.2 ± 0.047	3.556IleVal: 3.556 ± 0.079	0.523IleTrp: 0.523 ± 0.028	0.78IleTyr: 0.78 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.15LysAla: 4.15 ± 0.081	0.103LysCys: 0.103 ± 0.011	1.42LysAsp: 1.42 ± 0.049	1.03LysGlu: 1.03 ± 0.042	0.667LysPhe: 0.667 ± 0.027	2.306LysGly: 2.306 ± 0.053	0.457LysHis: 0.457 ± 0.026	1.183LysIle: 1.183 ± 0.041	0.978LysLys: 0.978 ± 0.042	2.334LysLeu: 2.334 ± 0.068	0.523LysMet: 0.523 ± 0.026	0.588LysAsn: 0.588 ± 0.026	1.771LysPro: 1.771 ± 0.056	0.723LysGln: 0.723 ± 0.03	2.075LysArg: 2.075 ± 0.051	1.425LysSer: 1.425 ± 0.043	1.693LysThr: 1.693 ± 0.057	1.893LysVal: 1.893 ± 0.059	0.3LysTrp: 0.3 ± 0.021	0.482LysTyr: 0.482 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	14.14LeuAla: 14.14 ± 0.171	0.707LeuCys: 0.707 ± 0.032	6.353LeuAsp: 6.353 ± 0.097	5.529LeuGlu: 5.529 ± 0.079	3.438LeuPhe: 3.438 ± 0.076	8.233LeuGly: 8.233 ± 0.111	1.603LeuHis: 1.603 ± 0.045	5.147LeuIle: 5.147 ± 0.088	3.553LeuLys: 3.553 ± 0.064	8.272LeuLeu: 8.272 ± 0.123	2.48LeuMet: 2.48 ± 0.059	2.873LeuAsn: 2.873 ± 0.068	5.014LeuPro: 5.014 ± 0.086	2.629LeuGln: 2.629 ± 0.063	6.886LeuArg: 6.886 ± 0.111	6.47LeuSer: 6.47 ± 0.105	6.384LeuThr: 6.384 ± 0.081	6.568LeuVal: 6.568 ± 0.103	1.223LeuTrp: 1.223 ± 0.042	1.844LeuTyr: 1.844 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	3.323MetAla: 3.323 ± 0.07	0.104MetCys: 0.104 ± 0.012	1.206MetAsp: 1.206 ± 0.041	0.978MetGlu: 0.978 ± 0.043	0.628MetPhe: 0.628 ± 0.032	1.921MetGly: 1.921 ± 0.054	0.377MetHis: 0.377 ± 0.023	1.163MetIle: 1.163 ± 0.035	0.854MetLys: 0.854 ± 0.03	1.997MetLeu: 1.997 ± 0.051	0.586MetMet: 0.586 ± 0.028	0.632MetAsn: 0.632 ± 0.027	1.11MetPro: 1.11 ± 0.036	0.712MetGln: 0.712 ± 0.032	1.768MetArg: 1.768 ± 0.052	1.541MetSer: 1.541 ± 0.046	1.902MetThr: 1.902 ± 0.052	1.423MetVal: 1.423 ± 0.047	0.22MetTrp: 0.22 ± 0.015	0.243MetTyr: 0.243 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.183AsnAla: 3.183 ± 0.072	0.161AsnCys: 0.161 ± 0.014	1.256AsnAsp: 1.256 ± 0.044	1.018AsnGlu: 1.018 ± 0.036	0.717AsnPhe: 0.717 ± 0.035	2.162AsnGly: 2.162 ± 0.058	0.422AsnHis: 0.422 ± 0.025	1.045AsnIle: 1.045 ± 0.037	0.465AsnLys: 0.465 ± 0.024	2.441AsnLeu: 2.441 ± 0.06	0.464AsnMet: 0.464 ± 0.023	0.567AsnAsn: 0.567 ± 0.031	1.684AsnPro: 1.684 ± 0.049	0.7AsnGln: 0.7 ± 0.03	1.783AsnArg: 1.783 ± 0.049	1.019AsnSer: 1.019 ± 0.038	1.071AsnThr: 1.071 ± 0.044	1.64AsnVal: 1.64 ± 0.047	0.319AsnTrp: 0.319 ± 0.021	0.553AsnTyr: 0.553 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	7.669ProAla: 7.669 ± 0.128	0.305ProCys: 0.305 ± 0.019	3.957ProAsp: 3.957 ± 0.078	3.898ProGlu: 3.898 ± 0.079	1.978ProPhe: 1.978 ± 0.053	5.056ProGly: 5.056 ± 0.096	0.962ProHis: 0.962 ± 0.037	2.292ProIle: 2.292 ± 0.051	1.24ProLys: 1.24 ± 0.043	4.724ProLeu: 4.724 ± 0.08	1.15ProMet: 1.15 ± 0.037	1.226ProAsn: 1.226 ± 0.043	3.03ProPro: 3.03 ± 0.106	1.781ProGln: 1.781 ± 0.054	3.578ProArg: 3.578 ± 0.064	2.906ProSer: 2.906 ± 0.06	2.876ProThr: 2.876 ± 0.057	4.295ProVal: 4.295 ± 0.072	0.811ProTrp: 0.811 ± 0.035	1.089ProTyr: 1.089 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	5.742GlnAla: 5.742 ± 0.081	0.185GlnCys: 0.185 ± 0.015	1.57GlnAsp: 1.57 ± 0.045	1.361GlnGlu: 1.361 ± 0.039	0.925GlnPhe: 0.925 ± 0.03	2.977GlnGly: 2.977 ± 0.061	0.522GlnHis: 0.522 ± 0.022	1.494GlnIle: 1.494 ± 0.038	0.831GlnLys: 0.831 ± 0.034	2.723GlnLeu: 2.723 ± 0.056	0.817GlnMet: 0.817 ± 0.031	0.729GlnAsn: 0.729 ± 0.03	2.121GlnPro: 2.121 ± 0.056	1.085GlnGln: 1.085 ± 0.039	2.467GlnArg: 2.467 ± 0.052	1.758GlnSer: 1.758 ± 0.044	2.109GlnThr: 2.109 ± 0.06	2.478GlnVal: 2.478 ± 0.05	0.385GlnTrp: 0.385 ± 0.021	0.512GlnTyr: 0.512 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	10.066ArgAla: 10.066 ± 0.133	0.457ArgCys: 0.457 ± 0.023	4.687ArgAsp: 4.687 ± 0.08	4.254ArgGlu: 4.254 ± 0.069	3.027ArgPhe: 3.027 ± 0.069	5.732ArgGly: 5.732 ± 0.084	1.386ArgHis: 1.386 ± 0.046	3.815ArgIle: 3.815 ± 0.069	1.864ArgLys: 1.864 ± 0.049	9.736ArgLeu: 9.736 ± 0.132	1.93ArgMet: 1.93 ± 0.052	1.647ArgAsn: 1.647 ± 0.048	4.735ArgPro: 4.735 ± 0.085	2.776ArgGln: 2.776 ± 0.055	7.172ArgArg: 7.172 ± 0.102	3.723ArgSer: 3.723 ± 0.072	3.764ArgThr: 3.764 ± 0.059	5.394ArgVal: 5.394 ± 0.083	1.262ArgTrp: 1.262 ± 0.043	1.704ArgTyr: 1.704 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	6.738SerAla: 6.738 ± 0.098	0.259SerCys: 0.259 ± 0.017	3.044SerAsp: 3.044 ± 0.057	2.517SerGlu: 2.517 ± 0.051	1.661SerPhe: 1.661 ± 0.048	5.39SerGly: 5.39 ± 0.087	0.849SerHis: 0.849 ± 0.031	2.17SerIle: 2.17 ± 0.052	1.223SerLys: 1.223 ± 0.043	5.07SerLeu: 5.07 ± 0.085	1.116SerMet: 1.116 ± 0.034	1.306SerAsn: 1.306 ± 0.045	3.057SerPro: 3.057 ± 0.059	1.686SerGln: 1.686 ± 0.042	3.849SerArg: 3.849 ± 0.069	2.552SerSer: 2.552 ± 0.062	2.627SerThr: 2.627 ± 0.062	3.784SerVal: 3.784 ± 0.068	0.752SerTrp: 0.752 ± 0.026	1.017SerTyr: 1.017 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	6.926ThrAla: 6.926 ± 0.1	0.354ThrCys: 0.354 ± 0.023	2.985ThrAsp: 2.985 ± 0.066	2.459ThrGlu: 2.459 ± 0.058	1.806ThrPhe: 1.806 ± 0.054	5.257ThrGly: 5.257 ± 0.088	0.836ThrHis: 0.836 ± 0.032	2.06ThrIle: 2.06 ± 0.057	1.057ThrLys: 1.057 ± 0.042	5.676ThrLeu: 5.676 ± 0.084	0.877ThrMet: 0.877 ± 0.032	1.151ThrAsn: 1.151 ± 0.046	3.965ThrPro: 3.965 ± 0.073	1.492ThrGln: 1.492 ± 0.048	3.573ThrArg: 3.573 ± 0.061	2.489ThrSer: 2.489 ± 0.06	2.827ThrThr: 2.827 ± 0.068	4.299ThrVal: 4.299 ± 0.074	0.662ThrTrp: 0.662 ± 0.029	1.119ThrTyr: 1.119 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	9.475ValAla: 9.475 ± 0.122	0.614ValCys: 0.614 ± 0.026	4.391ValAsp: 4.391 ± 0.084	5.232ValGlu: 5.232 ± 0.09	2.713ValPhe: 2.713 ± 0.059	6.039ValGly: 6.039 ± 0.085	1.294ValHis: 1.294 ± 0.04	3.659ValIle: 3.659 ± 0.065	1.973ValLys: 1.973 ± 0.054	7.539ValLeu: 7.539 ± 0.123	1.867ValMet: 1.867 ± 0.052	1.858ValAsn: 1.858 ± 0.061	2.88ValPro: 2.88 ± 0.059	2.423ValGln: 2.423 ± 0.066	5.784ValArg: 5.784 ± 0.087	4.259ValSer: 4.259 ± 0.084	4.489ValThr: 4.489 ± 0.081	6.541ValVal: 6.541 ± 0.111	1.136ValTrp: 1.136 ± 0.043	1.407ValTyr: 1.407 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.666TrpAla: 1.666 ± 0.052	0.119TrpCys: 0.119 ± 0.012	0.674TrpAsp: 0.674 ± 0.029	0.566TrpGlu: 0.566 ± 0.026	0.538TrpPhe: 0.538 ± 0.025	1.11TrpGly: 1.11 ± 0.038	0.235TrpHis: 0.235 ± 0.015	0.736TrpIle: 0.736 ± 0.028	0.451TrpLys: 0.451 ± 0.022	1.703TrpLeu: 1.703 ± 0.049	0.4TrpMet: 0.4 ± 0.022	0.416TrpAsn: 0.416 ± 0.026	0.796TrpPro: 0.796 ± 0.033	0.396TrpGln: 0.396 ± 0.023	1.514TrpArg: 1.514 ± 0.045	0.982TrpSer: 0.982 ± 0.034	1.01TrpThr: 1.01 ± 0.037	0.87TrpVal: 0.87 ± 0.03	0.25TrpTrp: 0.25 ± 0.02	0.223TrpTyr: 0.223 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.556TyrAla: 2.556 ± 0.063	0.168TyrCys: 0.168 ± 0.015	1.475TyrAsp: 1.475 ± 0.059	1.248TyrGlu: 1.248 ± 0.042	0.712TyrPhe: 0.712 ± 0.028	2.161TyrGly: 2.161 ± 0.058	0.36TyrHis: 0.36 ± 0.02	0.661TyrIle: 0.661 ± 0.03	0.387TyrLys: 0.387 ± 0.022	1.863TyrLeu: 1.863 ± 0.043	0.351TyrMet: 0.351 ± 0.021	0.443TyrAsn: 0.443 ± 0.023	0.948TyrPro: 0.948 ± 0.034	0.618TyrGln: 0.618 ± 0.027	1.729TyrArg: 1.729 ± 0.048	0.999TyrSer: 0.999 ± 0.041	0.848TyrThr: 0.848 ± 0.033	1.655TyrVal: 1.655 ± 0.042	0.295TyrTrp: 0.295 ± 0.02	0.458TyrTyr: 0.458 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2779 proteins (846945 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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