Marinovum algicola DG 898
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4976 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H4KPL3|A0A0H4KPL3_9RHOB RNA polymerase sigma factor OS=Marinovum algicola DG 898 OX=988812 GN=MALG_00285 PE=3 SV=1
MM1 pKa = 7.17 QRR3 pKa = 11.84 TKK5 pKa = 10.25 LTLGALVASTALAGMASAQTEE26 pKa = 4.18 LTLWYY31 pKa = 9.64 HH32 pKa = 5.79 GAGNEE37 pKa = 4.13 VEE39 pKa = 4.75 SNILGGIIEE48 pKa = 5.11 DD49 pKa = 3.8 FNGAQEE55 pKa = 4.17 DD56 pKa = 3.97 WQVTVEE62 pKa = 4.31 SFPQEE67 pKa = 4.15 SYY69 pKa = 10.96 NDD71 pKa = 3.8 SVVASALAGNLPCILDD87 pKa = 3.15 VDD89 pKa = 4.59 GPVMPNWAWAGYY101 pKa = 7.77 LQPLPISQEE110 pKa = 4.25 AIDD113 pKa = 3.91 GHH115 pKa = 8.4 LDD117 pKa = 3.28 ATIGRR122 pKa = 11.84 WDD124 pKa = 3.6 GEE126 pKa = 4.37 IYY128 pKa = 10.76 SVGLWDD134 pKa = 4.73 AAVALYY140 pKa = 10.38 ARR142 pKa = 11.84 QSTLDD147 pKa = 3.61 EE148 pKa = 4.93 LGLRR152 pKa = 11.84 TPSLEE157 pKa = 4.19 EE158 pKa = 3.68 PWTKK162 pKa = 10.92 EE163 pKa = 3.67 EE164 pKa = 4.75 FMGALEE170 pKa = 4.06 AAKK173 pKa = 10.66 ASGNYY178 pKa = 9.87 DD179 pKa = 3.26 FALDD183 pKa = 5.65 LGTAWTGEE191 pKa = 3.99 WYY193 pKa = 9.23 PYY195 pKa = 10.83 AFSPFLQSFGGDD207 pKa = 2.59 IVDD210 pKa = 3.49 RR211 pKa = 11.84 DD212 pKa = 4.19 GYY214 pKa = 8.65 QTAEE218 pKa = 3.84 GALNGEE224 pKa = 4.3 AAMEE228 pKa = 4.63 FGAWWQSLFAEE239 pKa = 6.43 GYY241 pKa = 10.97 APGTSQDD248 pKa = 3.48 PADD251 pKa = 4.56 RR252 pKa = 11.84 DD253 pKa = 3.66 SGFIEE258 pKa = 4.36 GKK260 pKa = 10.4 YY261 pKa = 9.64 AFSWNGNWAAANTLASVEE279 pKa = 4.2 NADD282 pKa = 4.73 DD283 pKa = 4.38 VVFLPAPDD291 pKa = 4.36 FGNGSTIGAASWQFGVSSACEE312 pKa = 4.05 HH313 pKa = 7.1 PEE315 pKa = 3.61 GAAAFVEE322 pKa = 4.61 HH323 pKa = 7.15 ALQDD327 pKa = 3.49 EE328 pKa = 4.49 YY329 pKa = 11.71 LAAFSDD335 pKa = 5.04 GIGLIPATKK344 pKa = 9.67 EE345 pKa = 3.43 AAQMTEE351 pKa = 4.3 SYY353 pKa = 11.58 AEE355 pKa = 4.32 GGPLAVFFDD364 pKa = 4.56 LSAEE368 pKa = 4.13 QALVRR373 pKa = 11.84 PVSPGYY379 pKa = 10.29 VVAAKK384 pKa = 10.41 VFEE387 pKa = 4.44 KK388 pKa = 10.82 ALSDD392 pKa = 3.46 IANGADD398 pKa = 3.57 VADD401 pKa = 4.21 TLDD404 pKa = 3.73 AAVDD408 pKa = 4.35 EE409 pKa = 4.86 IDD411 pKa = 5.8 DD412 pKa = 5.59 DD413 pKa = 4.13 IEE415 pKa = 4.99 ANQGYY420 pKa = 8.33 GHH422 pKa = 7.53
Molecular weight: 44.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.516
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.427
Solomon 3.706
Lehninger 3.656
Nozaki 3.821
DTASelect 4.024
Thurlkill 3.554
EMBOSS 3.63
Sillero 3.846
Patrickios 0.985
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A0H4KZ01|A0A0H4KZ01_9RHOB Serine phosphatase RsbU regulator of sigma subunit OS=Marinovum algicola DG 898 OX=988812 GN=MALG_03562 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4976
0
4976
1570877
30
2735
315.7
34.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.847 ± 0.055
0.92 ± 0.01
5.967 ± 0.035
6.042 ± 0.035
3.698 ± 0.021
8.87 ± 0.032
2.062 ± 0.017
4.957 ± 0.026
2.86 ± 0.027
10.188 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.699 ± 0.018
2.427 ± 0.02
5.2 ± 0.026
3.137 ± 0.016
7.048 ± 0.038
4.918 ± 0.023
5.353 ± 0.025
7.217 ± 0.033
1.398 ± 0.014
2.193 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here