Pyrodictium occultum

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Pyrodictiaceae; Pyrodictium

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1602 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0V8RV83|A0A0V8RV83_PYROC Uncharacterized protein OS=Pyrodictium occultum OX=2309 GN=CF15_03875 PE=4 SV=1
MM1 pKa = 7.71GLRR4 pKa = 11.84LTTTRR9 pKa = 11.84YY10 pKa = 9.62FSIPYY15 pKa = 10.0SVFDD19 pKa = 3.74VLLQMRR25 pKa = 11.84GAIDD29 pKa = 4.12PDD31 pKa = 3.38SGQIVCHH38 pKa = 6.69FYY40 pKa = 11.37DD41 pKa = 3.8LGVAVLEE48 pKa = 4.55SPGAAVPVIVVEE60 pKa = 4.5GCLLATFGDD69 pKa = 4.3EE70 pKa = 4.27NVKK73 pKa = 10.75ALLDD77 pKa = 3.75SGSAVEE83 pKa = 4.13VQEE86 pKa = 4.98LRR88 pKa = 11.84VTQGRR93 pKa = 11.84VMVGDD98 pKa = 3.63VEE100 pKa = 5.29IDD102 pKa = 3.8HH103 pKa = 6.65FWGLAALAYY112 pKa = 10.23AGEE115 pKa = 4.37TGAEE119 pKa = 3.93VKK121 pKa = 10.29LYY123 pKa = 10.68KK124 pKa = 10.39LRR126 pKa = 11.84LDD128 pKa = 3.31VDD130 pKa = 3.71GFEE133 pKa = 4.62APVAGVYY140 pKa = 10.29VSSTDD145 pKa = 3.4AGPMLMLVPRR155 pKa = 11.84SCEE158 pKa = 3.72YY159 pKa = 10.34IEE161 pKa = 4.74EE162 pKa = 4.25QEE164 pKa = 4.41QPPTHH169 pKa = 5.82MM170 pKa = 5.26

Molecular weight: 18.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0V8RUK9|A0A0V8RUK9_PYROC Uncharacterized protein OS=Pyrodictium occultum OX=2309 GN=CF15_02715 PE=4 SV=1
MM1 pKa = 7.72ARR3 pKa = 11.84FKK5 pKa = 10.96PLGKK9 pKa = 10.1KK10 pKa = 9.8LRR12 pKa = 11.84LARR15 pKa = 11.84ALKK18 pKa = 9.94QNRR21 pKa = 11.84AVPIWVVIRR30 pKa = 11.84TSRR33 pKa = 11.84RR34 pKa = 11.84FRR36 pKa = 11.84DD37 pKa = 3.5HH38 pKa = 6.87PKK40 pKa = 8.22RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.75WRR45 pKa = 11.84RR46 pKa = 11.84VKK48 pKa = 10.84LKK50 pKa = 10.56AA51 pKa = 3.65

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1602

0

1602

456718

48

1504

285.1

31.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.145 ± 0.096

0.839 ± 0.025

4.053 ± 0.037

7.767 ± 0.065

2.356 ± 0.033

8.736 ± 0.065

1.755 ± 0.02

4.771 ± 0.063

3.5 ± 0.068

11.65 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.022

1.775 ± 0.037

5.449 ± 0.037

1.808 ± 0.029

9.11 ± 0.075

5.361 ± 0.048

3.875 ± 0.052

9.041 ± 0.056

1.264 ± 0.023

3.597 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski