Bacillus phage Karezi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Tatarstanvirinae; Karezivirus; Bacillus virus Karezi

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514AAP6|A0A514AAP6_9CAUD Morphogenesis protein OS=Bacillus phage Karezi OX=2591398 GN=KAREZI_21 PE=4 SV=1
MM1 pKa = 6.13TQWVISVGKK10 pKa = 8.98MFYY13 pKa = 10.98AEE15 pKa = 4.14GTQLVTEE22 pKa = 4.67LEE24 pKa = 4.5YY25 pKa = 11.22AFHH28 pKa = 7.13FNIKK32 pKa = 10.08SEE34 pKa = 4.01ALEE37 pKa = 3.94IVDD40 pKa = 3.12WLEE43 pKa = 3.58YY44 pKa = 10.87RR45 pKa = 11.84EE46 pKa = 4.12VEE48 pKa = 4.01NTNLVEE54 pKa = 3.91ITTMQEE60 pKa = 3.3EE61 pKa = 4.25NTYY64 pKa = 11.05KK65 pKa = 10.57EE66 pKa = 3.88ISDD69 pKa = 4.43LFDD72 pKa = 3.44AQRR75 pKa = 11.84QKK77 pKa = 11.82GEE79 pKa = 3.95MKK81 pKa = 10.72YY82 pKa = 10.75GGTVDD87 pKa = 4.43EE88 pKa = 5.09QDD90 pKa = 3.78NDD92 pKa = 4.11DD93 pKa = 3.51PVYY96 pKa = 9.56WVNHH100 pKa = 4.81ARR102 pKa = 11.84EE103 pKa = 4.2EE104 pKa = 4.34VADD107 pKa = 4.09LLVYY111 pKa = 8.65LTKK114 pKa = 10.78LKK116 pKa = 10.73QKK118 pKa = 10.69LEE120 pKa = 4.16KK121 pKa = 10.1LDD123 pKa = 3.65KK124 pKa = 11.07

Molecular weight: 14.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514AAR7|A0A514AAR7_9CAUD Uncharacterized protein OS=Bacillus phage Karezi OX=2591398 GN=KAREZI_29 PE=4 SV=1
MM1 pKa = 6.73VACTIITSMCFIPSPLSVAKK21 pKa = 10.36RR22 pKa = 11.84RR23 pKa = 11.84EE24 pKa = 3.85QGRR27 pKa = 11.84LYY29 pKa = 10.87HH30 pKa = 6.18GVAYY34 pKa = 8.26PALFPPSYY42 pKa = 10.65YY43 pKa = 9.81IRR45 pKa = 11.84NKK47 pKa = 7.82NTRR50 pKa = 11.84KK51 pKa = 9.81KK52 pKa = 10.81DD53 pKa = 3.41LL54 pKa = 3.94

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

6241

28

655

183.6

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.464 ± 0.462

0.817 ± 0.181

5.88 ± 0.336

7.098 ± 0.471

5.063 ± 0.358

6.473 ± 0.55

1.875 ± 0.182

6.682 ± 0.349

7.515 ± 0.565

7.659 ± 0.377

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.9 ± 0.184

6.361 ± 0.499

2.804 ± 0.273

3.813 ± 0.316

4.326 ± 0.316

5.592 ± 0.232

6.602 ± 0.427

6.954 ± 0.479

1.074 ± 0.214

5.047 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski