Amino acid dipepetide frequency for Bacillus phage Karezi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.763AlaAla: 1.763 ± 0.546
0.641AlaCys: 0.641 ± 0.281
3.846AlaAsp: 3.846 ± 0.614
3.526AlaGlu: 3.526 ± 0.764
3.365AlaPhe: 3.365 ± 1.011
4.647AlaGly: 4.647 ± 0.835
0.801AlaHis: 0.801 ± 0.331
3.365AlaIle: 3.365 ± 0.811
3.205AlaLys: 3.205 ± 0.705
4.006AlaLeu: 4.006 ± 0.735
1.603AlaMet: 1.603 ± 0.578
4.006AlaAsn: 4.006 ± 1.14
1.442AlaPro: 1.442 ± 0.637
2.404AlaGln: 2.404 ± 0.549
2.885AlaArg: 2.885 ± 0.494
3.205AlaSer: 3.205 ± 1.235
4.006AlaThr: 4.006 ± 0.697
3.045AlaVal: 3.045 ± 0.943
0.481AlaTrp: 0.481 ± 0.261
2.404AlaTyr: 2.404 ± 0.594
0.0AlaXaa: 0.0 ± 0.0
Cys
0.801CysAla: 0.801 ± 0.292
0.0CysCys: 0.0 ± 0.0
0.321CysAsp: 0.321 ± 0.253
0.481CysGlu: 0.481 ± 0.237
0.481CysPhe: 0.481 ± 0.274
0.641CysGly: 0.641 ± 0.444
0.321CysHis: 0.321 ± 0.305
0.481CysIle: 0.481 ± 0.238
0.481CysLys: 0.481 ± 0.264
0.321CysLeu: 0.321 ± 0.246
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.0CysPro: 0.0 ± 0.0
0.16CysGln: 0.16 ± 0.182
0.641CysArg: 0.641 ± 0.453
0.16CysSer: 0.16 ± 0.152
0.321CysThr: 0.321 ± 0.211
0.962CysVal: 0.962 ± 0.457
0.16CysTrp: 0.16 ± 0.157
1.442CysTyr: 1.442 ± 0.394
0.0CysXaa: 0.0 ± 0.0
Asp
3.526AspAla: 3.526 ± 0.731
1.282AspCys: 1.282 ± 0.741
4.006AspAsp: 4.006 ± 0.92
4.327AspGlu: 4.327 ± 0.864
4.327AspPhe: 4.327 ± 0.815
4.487AspGly: 4.487 ± 1.058
1.763AspHis: 1.763 ± 0.845
3.526AspIle: 3.526 ± 0.834
3.365AspLys: 3.365 ± 0.902
5.128AspLeu: 5.128 ± 0.59
1.122AspMet: 1.122 ± 0.439
1.603AspAsn: 1.603 ± 0.526
2.244AspPro: 2.244 ± 0.671
1.603AspGln: 1.603 ± 0.525
2.724AspArg: 2.724 ± 0.691
2.724AspSer: 2.724 ± 0.718
4.167AspThr: 4.167 ± 0.856
3.365AspVal: 3.365 ± 0.735
0.16AspTrp: 0.16 ± 0.181
3.365AspTyr: 3.365 ± 0.896
0.0AspXaa: 0.0 ± 0.0
Glu
4.167GluAla: 4.167 ± 0.824
0.641GluCys: 0.641 ± 0.306
3.205GluAsp: 3.205 ± 0.664
5.609GluGlu: 5.609 ± 1.318
2.083GluPhe: 2.083 ± 0.516
4.327GluGly: 4.327 ± 1.059
1.442GluHis: 1.442 ± 0.504
4.647GluIle: 4.647 ± 0.855
5.128GluLys: 5.128 ± 1.148
6.731GluLeu: 6.731 ± 1.593
2.404GluMet: 2.404 ± 0.474
6.09GluAsn: 6.09 ± 1.012
0.641GluPro: 0.641 ± 0.303
1.923GluGln: 1.923 ± 0.783
1.923GluArg: 1.923 ± 0.641
3.365GluSer: 3.365 ± 0.523
4.647GluThr: 4.647 ± 1.073
6.25GluVal: 6.25 ± 1.231
1.442GluTrp: 1.442 ± 0.473
4.327GluTyr: 4.327 ± 0.884
0.0GluXaa: 0.0 ± 0.0
Phe
2.244PheAla: 2.244 ± 0.721
0.481PheCys: 0.481 ± 0.379
2.724PheAsp: 2.724 ± 0.723
4.647PheGlu: 4.647 ± 0.849
4.006PhePhe: 4.006 ± 1.007
3.205PheGly: 3.205 ± 0.965
0.801PheHis: 0.801 ± 0.367
5.609PheIle: 5.609 ± 0.81
5.769PheLys: 5.769 ± 1.151
3.045PheLeu: 3.045 ± 0.825
0.481PheMet: 0.481 ± 0.251
3.526PheAsn: 3.526 ± 0.696
1.603PhePro: 1.603 ± 0.437
0.641PheGln: 0.641 ± 0.362
2.083PheArg: 2.083 ± 0.654
2.885PheSer: 2.885 ± 0.616
3.846PheThr: 3.846 ± 0.967
1.122PheVal: 1.122 ± 0.375
0.16PheTrp: 0.16 ± 0.177
1.763PheTyr: 1.763 ± 0.453
0.0PheXaa: 0.0 ± 0.0
Gly
3.205GlyAla: 3.205 ± 0.869
0.801GlyCys: 0.801 ± 0.373
3.526GlyAsp: 3.526 ± 0.906
3.045GlyGlu: 3.045 ± 0.712
3.365GlyPhe: 3.365 ± 0.851
5.769GlyGly: 5.769 ± 1.614
0.801GlyHis: 0.801 ± 0.312
4.006GlyIle: 4.006 ± 0.973
4.968GlyLys: 4.968 ± 0.942
5.609GlyLeu: 5.609 ± 1.044
2.564GlyMet: 2.564 ± 0.794
5.128GlyAsn: 5.128 ± 1.195
0.0GlyPro: 0.0 ± 0.0
2.564GlyGln: 2.564 ± 0.475
3.526GlyArg: 3.526 ± 0.767
3.205GlySer: 3.205 ± 1.187
3.045GlyThr: 3.045 ± 0.703
5.609GlyVal: 5.609 ± 1.246
0.962GlyTrp: 0.962 ± 0.43
3.045GlyTyr: 3.045 ± 0.773
0.0GlyXaa: 0.0 ± 0.0
His
0.481HisAla: 0.481 ± 0.352
0.0HisCys: 0.0 ± 0.0
1.282HisAsp: 1.282 ± 0.346
1.282HisGlu: 1.282 ± 0.378
0.962HisPhe: 0.962 ± 0.545
1.282HisGly: 1.282 ± 0.721
0.321HisHis: 0.321 ± 0.217
1.603HisIle: 1.603 ± 0.404
1.442HisLys: 1.442 ± 0.523
1.442HisLeu: 1.442 ± 0.49
0.801HisMet: 0.801 ± 0.322
1.282HisAsn: 1.282 ± 0.462
0.641HisPro: 0.641 ± 0.238
0.801HisGln: 0.801 ± 0.344
0.481HisArg: 0.481 ± 0.265
0.16HisSer: 0.16 ± 0.172
0.801HisThr: 0.801 ± 0.49
1.923HisVal: 1.923 ± 0.703
0.16HisTrp: 0.16 ± 0.171
1.603HisTyr: 1.603 ± 0.434
0.0HisXaa: 0.0 ± 0.0
Ile
3.846IleAla: 3.846 ± 0.646
0.16IleCys: 0.16 ± 0.177
4.006IleAsp: 4.006 ± 0.995
5.288IleGlu: 5.288 ± 1.028
2.564IlePhe: 2.564 ± 0.641
4.808IleGly: 4.808 ± 0.893
1.442IleHis: 1.442 ± 0.703
4.167IleIle: 4.167 ± 0.986
4.968IleLys: 4.968 ± 1.003
4.647IleLeu: 4.647 ± 1.014
0.801IleMet: 0.801 ± 0.421
4.647IleAsn: 4.647 ± 0.693
2.404IlePro: 2.404 ± 0.499
2.885IleGln: 2.885 ± 0.701
3.365IleArg: 3.365 ± 0.744
2.404IleSer: 2.404 ± 0.665
4.487IleThr: 4.487 ± 1.04
4.327IleVal: 4.327 ± 0.804
0.641IleTrp: 0.641 ± 0.43
4.808IleTyr: 4.808 ± 0.843
0.0IleXaa: 0.0 ± 0.0
Lys
3.205LysAla: 3.205 ± 1.038
0.641LysCys: 0.641 ± 0.28
4.647LysAsp: 4.647 ± 1.044
5.288LysGlu: 5.288 ± 1.026
4.327LysPhe: 4.327 ± 0.977
6.41LysGly: 6.41 ± 1.038
1.442LysHis: 1.442 ± 0.563
4.167LysIle: 4.167 ± 0.799
6.25LysLys: 6.25 ± 1.78
8.494LysLeu: 8.494 ± 1.672
3.045LysMet: 3.045 ± 0.498
4.167LysAsn: 4.167 ± 0.822
2.244LysPro: 2.244 ± 0.604
3.365LysGln: 3.365 ± 0.904
3.205LysArg: 3.205 ± 0.706
4.327LysSer: 4.327 ± 1.206
4.647LysThr: 4.647 ± 0.88
3.045LysVal: 3.045 ± 0.596
0.641LysTrp: 0.641 ± 0.255
1.603LysTyr: 1.603 ± 0.531
0.0LysXaa: 0.0 ± 0.0
Leu
4.968LeuAla: 4.968 ± 0.802
0.481LeuCys: 0.481 ± 0.308
3.686LeuAsp: 3.686 ± 0.723
5.449LeuGlu: 5.449 ± 1.703
2.724LeuPhe: 2.724 ± 0.695
3.526LeuGly: 3.526 ± 0.633
1.442LeuHis: 1.442 ± 0.525
3.686LeuIle: 3.686 ± 0.616
6.09LeuLys: 6.09 ± 1.337
5.769LeuLeu: 5.769 ± 1.094
2.564LeuMet: 2.564 ± 0.661
5.128LeuAsn: 5.128 ± 1.106
2.724LeuPro: 2.724 ± 0.771
3.205LeuGln: 3.205 ± 0.675
4.006LeuArg: 4.006 ± 0.84
6.571LeuSer: 6.571 ± 1.385
3.846LeuThr: 3.846 ± 0.753
5.609LeuVal: 5.609 ± 0.903
0.641LeuTrp: 0.641 ± 0.352
4.487LeuTyr: 4.487 ± 1.091
0.0LeuXaa: 0.0 ± 0.0
Met
1.122MetAla: 1.122 ± 0.339
0.481MetCys: 0.481 ± 0.31
2.244MetAsp: 2.244 ± 0.685
1.442MetGlu: 1.442 ± 0.454
1.282MetPhe: 1.282 ± 0.471
1.923MetGly: 1.923 ± 0.581
0.481MetHis: 0.481 ± 0.246
2.885MetIle: 2.885 ± 0.848
1.603MetLys: 1.603 ± 0.53
2.083MetLeu: 2.083 ± 0.563
1.282MetMet: 1.282 ± 0.542
1.122MetAsn: 1.122 ± 0.632
0.801MetPro: 0.801 ± 0.445
1.122MetGln: 1.122 ± 0.417
1.122MetArg: 1.122 ± 0.515
1.603MetSer: 1.603 ± 0.503
3.205MetThr: 3.205 ± 0.724
1.282MetVal: 1.282 ± 0.523
0.16MetTrp: 0.16 ± 0.158
1.763MetTyr: 1.763 ± 0.632
0.0MetXaa: 0.0 ± 0.0
Asn
4.808AsnAla: 4.808 ± 1.371
0.321AsnCys: 0.321 ± 0.205
4.167AsnAsp: 4.167 ± 0.757
3.686AsnGlu: 3.686 ± 0.77
2.564AsnPhe: 2.564 ± 0.7
5.128AsnGly: 5.128 ± 1.313
0.962AsnHis: 0.962 ± 0.53
3.686AsnIle: 3.686 ± 0.928
4.327AsnLys: 4.327 ± 0.871
3.846AsnLeu: 3.846 ± 0.713
1.923AsnMet: 1.923 ± 0.545
2.885AsnAsn: 2.885 ± 0.926
2.404AsnPro: 2.404 ± 0.661
2.564AsnGln: 2.564 ± 1.101
2.724AsnArg: 2.724 ± 0.611
4.808AsnSer: 4.808 ± 0.912
4.647AsnThr: 4.647 ± 1.012
4.006AsnVal: 4.006 ± 0.925
1.122AsnTrp: 1.122 ± 0.489
3.045AsnTyr: 3.045 ± 0.643
0.0AsnXaa: 0.0 ± 0.0
Pro
2.083ProAla: 2.083 ± 0.5
0.321ProCys: 0.321 ± 0.209
1.763ProAsp: 1.763 ± 0.77
1.763ProGlu: 1.763 ± 0.577
1.282ProPhe: 1.282 ± 0.442
0.321ProGly: 0.321 ± 0.342
0.16ProHis: 0.16 ± 0.184
2.083ProIle: 2.083 ± 0.605
2.083ProLys: 2.083 ± 0.576
1.923ProLeu: 1.923 ± 0.415
0.641ProMet: 0.641 ± 0.247
2.244ProAsn: 2.244 ± 0.523
1.122ProPro: 1.122 ± 0.416
1.923ProGln: 1.923 ± 0.589
0.641ProArg: 0.641 ± 0.325
2.244ProSer: 2.244 ± 0.551
0.801ProThr: 0.801 ± 0.279
2.404ProVal: 2.404 ± 0.6
0.321ProTrp: 0.321 ± 0.253
1.923ProTyr: 1.923 ± 0.598
0.0ProXaa: 0.0 ± 0.0
Gln
1.923GlnAla: 1.923 ± 0.447
0.0GlnCys: 0.0 ± 0.0
1.923GlnAsp: 1.923 ± 0.5
3.045GlnGlu: 3.045 ± 0.846
3.205GlnPhe: 3.205 ± 0.529
1.923GlnGly: 1.923 ± 0.475
0.801GlnHis: 0.801 ± 0.429
2.244GlnIle: 2.244 ± 0.733
3.045GlnLys: 3.045 ± 0.603
2.404GlnLeu: 2.404 ± 0.555
1.442GlnMet: 1.442 ± 0.588
2.404GlnAsn: 2.404 ± 0.686
1.763GlnPro: 1.763 ± 0.584
1.442GlnGln: 1.442 ± 0.519
1.923GlnArg: 1.923 ± 0.771
2.244GlnSer: 2.244 ± 0.593
2.404GlnThr: 2.404 ± 0.553
1.763GlnVal: 1.763 ± 0.585
1.122GlnTrp: 1.122 ± 0.423
1.122GlnTyr: 1.122 ± 0.38
0.0GlnXaa: 0.0 ± 0.0
Arg
2.083ArgAla: 2.083 ± 0.67
0.16ArgCys: 0.16 ± 0.152
2.083ArgAsp: 2.083 ± 0.591
4.327ArgGlu: 4.327 ± 1.03
3.045ArgPhe: 3.045 ± 0.649
1.923ArgGly: 1.923 ± 0.584
0.801ArgHis: 0.801 ± 0.431
3.686ArgIle: 3.686 ± 0.85
3.365ArgLys: 3.365 ± 0.765
2.724ArgLeu: 2.724 ± 0.694
1.122ArgMet: 1.122 ± 0.397
3.205ArgAsn: 3.205 ± 0.598
0.801ArgPro: 0.801 ± 0.369
1.603ArgGln: 1.603 ± 0.452
1.442ArgArg: 1.442 ± 0.524
2.083ArgSer: 2.083 ± 0.649
2.564ArgThr: 2.564 ± 0.523
4.006ArgVal: 4.006 ± 0.516
0.481ArgTrp: 0.481 ± 0.374
1.763ArgTyr: 1.763 ± 0.687
0.0ArgXaa: 0.0 ± 0.0
Ser
2.885SerAla: 2.885 ± 0.901
0.481SerCys: 0.481 ± 0.273
3.846SerAsp: 3.846 ± 0.778
3.846SerGlu: 3.846 ± 0.776
2.564SerPhe: 2.564 ± 0.597
3.205SerGly: 3.205 ± 0.84
0.962SerHis: 0.962 ± 0.495
4.487SerIle: 4.487 ± 0.953
3.686SerLys: 3.686 ± 0.593
3.686SerLeu: 3.686 ± 0.868
1.122SerMet: 1.122 ± 0.37
4.006SerAsn: 4.006 ± 1.402
1.122SerPro: 1.122 ± 0.618
2.083SerGln: 2.083 ± 0.587
2.885SerArg: 2.885 ± 1.033
3.846SerSer: 3.846 ± 0.768
3.526SerThr: 3.526 ± 0.787
4.808SerVal: 4.808 ± 0.925
0.321SerTrp: 0.321 ± 0.213
2.564SerTyr: 2.564 ± 0.862
0.0SerXaa: 0.0 ± 0.0
Thr
4.006ThrAla: 4.006 ± 0.976
0.321ThrCys: 0.321 ± 0.239
3.686ThrAsp: 3.686 ± 0.797
3.526ThrGlu: 3.526 ± 0.793
2.564ThrPhe: 2.564 ± 0.69
5.769ThrGly: 5.769 ± 0.833
1.603ThrHis: 1.603 ± 0.37
4.487ThrIle: 4.487 ± 0.921
4.327ThrLys: 4.327 ± 1.15
5.449ThrLeu: 5.449 ± 1.148
1.282ThrMet: 1.282 ± 0.414
3.365ThrAsn: 3.365 ± 0.711
2.404ThrPro: 2.404 ± 0.542
2.404ThrGln: 2.404 ± 0.657
3.205ThrArg: 3.205 ± 0.766
4.647ThrSer: 4.647 ± 0.991
4.327ThrThr: 4.327 ± 0.915
5.288ThrVal: 5.288 ± 0.858
0.16ThrTrp: 0.16 ± 0.152
3.205ThrTyr: 3.205 ± 0.69
0.0ThrXaa: 0.0 ± 0.0
Val
4.487ValAla: 4.487 ± 0.854
0.641ValCys: 0.641 ± 0.342
3.365ValAsp: 3.365 ± 0.683
5.929ValGlu: 5.929 ± 0.938
2.885ValPhe: 2.885 ± 0.743
2.244ValGly: 2.244 ± 0.55
1.282ValHis: 1.282 ± 0.5
4.487ValIle: 4.487 ± 0.907
5.769ValLys: 5.769 ± 0.992
4.487ValLeu: 4.487 ± 1.001
2.564ValMet: 2.564 ± 0.744
4.167ValAsn: 4.167 ± 1.108
1.603ValPro: 1.603 ± 0.648
3.205ValGln: 3.205 ± 0.986
2.564ValArg: 2.564 ± 0.626
3.526ValSer: 3.526 ± 0.639
6.41ValThr: 6.41 ± 1.109
5.128ValVal: 5.128 ± 1.09
0.801ValTrp: 0.801 ± 0.51
4.006ValTyr: 4.006 ± 0.818
0.0ValXaa: 0.0 ± 0.0
Trp
0.801TrpAla: 0.801 ± 0.287
0.0TrpCys: 0.0 ± 0.0
0.16TrpAsp: 0.16 ± 0.152
0.641TrpGlu: 0.641 ± 0.442
0.481TrpPhe: 0.481 ± 0.321
0.321TrpGly: 0.321 ± 0.213
0.321TrpHis: 0.321 ± 0.21
0.16TrpIle: 0.16 ± 0.171
1.122TrpLys: 1.122 ± 0.498
0.962TrpLeu: 0.962 ± 0.379
0.641TrpMet: 0.641 ± 0.305
0.641TrpAsn: 0.641 ± 0.288
0.0TrpPro: 0.0 ± 0.0
0.641TrpGln: 0.641 ± 0.281
0.481TrpArg: 0.481 ± 0.274
0.641TrpSer: 0.641 ± 0.306
0.641TrpThr: 0.641 ± 0.416
1.603TrpVal: 1.603 ± 0.521
0.0TrpTrp: 0.0 ± 0.0
0.481TrpTyr: 0.481 ± 0.234
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.244TyrAla: 2.244 ± 0.612
0.321TyrCys: 0.321 ± 0.236
4.327TyrAsp: 4.327 ± 1.011
3.846TyrGlu: 3.846 ± 0.798
2.564TyrPhe: 2.564 ± 0.595
2.885TyrGly: 2.885 ± 0.647
0.801TyrHis: 0.801 ± 0.361
3.365TyrIle: 3.365 ± 0.834
4.167TyrLys: 4.167 ± 0.776
3.846TyrLeu: 3.846 ± 0.826
1.442TyrMet: 1.442 ± 0.526
4.006TyrAsn: 4.006 ± 0.651
2.083TyrPro: 2.083 ± 0.504
1.603TyrGln: 1.603 ± 0.736
1.442TyrArg: 1.442 ± 0.534
1.282TyrSer: 1.282 ± 0.452
3.686TyrThr: 3.686 ± 0.766
4.006TyrVal: 4.006 ± 0.916
0.801TyrTrp: 0.801 ± 0.27
1.763TyrTyr: 1.763 ± 0.489
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 34 proteins (6241 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski