Streptomyces sp. ms191

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6788 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A652L2A6|A0A652L2A6_9ACTN Uncharacterized protein OS=Streptomyces sp. ms191 OX=1827978 GN=EAO71_15995 PE=4 SV=1
MM1 pKa = 7.68AFLLTSCSVYY11 pKa = 10.04PVEE14 pKa = 5.31VDD16 pKa = 4.16TVDD19 pKa = 3.23QLAGVWSDD27 pKa = 3.18FDD29 pKa = 3.52GRR31 pKa = 11.84TVEE34 pKa = 4.81FKK36 pKa = 11.08DD37 pKa = 4.35DD38 pKa = 3.79GTFTAQGLDD47 pKa = 3.36EE48 pKa = 5.17AGIGAQCSGIAARR61 pKa = 11.84QHH63 pKa = 5.92GAVSLSGTYY72 pKa = 10.92GEE74 pKa = 4.46VTFDD78 pKa = 3.99GVDD81 pKa = 3.63CEE83 pKa = 4.46GMALAFYY90 pKa = 9.77GSPSSFVACFTRR102 pKa = 11.84DD103 pKa = 3.66PVSGGCTDD111 pKa = 3.47EE112 pKa = 4.92FSRR115 pKa = 11.84KK116 pKa = 9.36ADD118 pKa = 3.93PGDD121 pKa = 3.84PGGAPTTT128 pKa = 4.0

Molecular weight: 13.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A652L497|A0A652L497_9ACTN DUF2617 family protein OS=Streptomyces sp. ms191 OX=1827978 GN=EAO71_19775 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6788

0

6788

2132704

20

4779

314.2

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.865 ± 0.048

0.789 ± 0.009

5.962 ± 0.024

5.728 ± 0.03

2.721 ± 0.015

9.661 ± 0.029

2.24 ± 0.016

3.059 ± 0.022

2.075 ± 0.028

10.386 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.708 ± 0.012

1.692 ± 0.017

6.112 ± 0.026

2.591 ± 0.019

8.189 ± 0.035

4.919 ± 0.02

6.154 ± 0.028

8.571 ± 0.029

1.515 ± 0.013

2.048 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski