Amino acid dipepetide frequency for Streptomyces sp. ms191

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.398AlaAla: 22.398 ± 0.173	1.096AlaCys: 1.096 ± 0.024	8.454AlaAsp: 8.454 ± 0.067	8.975AlaGlu: 8.975 ± 0.084	3.588AlaPhe: 3.588 ± 0.044	13.956AlaGly: 13.956 ± 0.111	2.845AlaHis: 2.845 ± 0.039	3.328AlaIle: 3.328 ± 0.04	2.77AlaLys: 2.77 ± 0.048	14.738AlaLeu: 14.738 ± 0.113	2.489AlaMet: 2.489 ± 0.037	1.833AlaAsn: 1.833 ± 0.034	7.382AlaPro: 7.382 ± 0.084	3.568AlaGln: 3.568 ± 0.049	10.708AlaArg: 10.708 ± 0.086	5.887AlaSer: 5.887 ± 0.052	7.208AlaThr: 7.208 ± 0.057	12.672AlaVal: 12.672 ± 0.098	1.921AlaTrp: 1.921 ± 0.031	2.805AlaTyr: 2.805 ± 0.038	0.036AlaXaa: 0.036 ± 0.004
Cys	1.119CysAla: 1.119 ± 0.025	0.096CysCys: 0.096 ± 0.007	0.454CysAsp: 0.454 ± 0.015	0.399CysGlu: 0.399 ± 0.013	0.245CysPhe: 0.245 ± 0.01	0.994CysGly: 0.994 ± 0.023	0.188CysHis: 0.188 ± 0.01	0.167CysIle: 0.167 ± 0.009	0.114CysLys: 0.114 ± 0.008	0.738CysLeu: 0.738 ± 0.02	0.129CysMet: 0.129 ± 0.008	0.141CysAsn: 0.141 ± 0.008	0.491CysPro: 0.491 ± 0.016	0.144CysGln: 0.144 ± 0.007	0.622CysArg: 0.622 ± 0.016	0.449CysSer: 0.449 ± 0.014	0.488CysThr: 0.488 ± 0.016	0.652CysVal: 0.652 ± 0.016	0.119CysTrp: 0.119 ± 0.008	0.14CysTyr: 0.14 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.912AspAla: 7.912 ± 0.061	0.429AspCys: 0.429 ± 0.015	3.46AspAsp: 3.46 ± 0.042	3.984AspGlu: 3.984 ± 0.053	1.628AspPhe: 1.628 ± 0.028	6.567AspGly: 6.567 ± 0.063	1.43AspHis: 1.43 ± 0.028	1.726AspIle: 1.726 ± 0.031	1.137AspLys: 1.137 ± 0.031	6.188AspLeu: 6.188 ± 0.054	0.801AspMet: 0.801 ± 0.023	0.902AspAsn: 0.902 ± 0.024	4.614AspPro: 4.614 ± 0.047	1.39AspGln: 1.39 ± 0.027	5.121AspArg: 5.121 ± 0.057	2.378AspSer: 2.378 ± 0.032	3.203AspThr: 3.203 ± 0.04	4.637AspVal: 4.637 ± 0.052	1.045AspTrp: 1.045 ± 0.022	1.059AspTyr: 1.059 ± 0.024	0.008AspXaa: 0.008 ± 0.002
Glu	7.726GluAla: 7.726 ± 0.087	0.347GluCys: 0.347 ± 0.011	2.936GluAsp: 2.936 ± 0.041	3.642GluGlu: 3.642 ± 0.067	1.446GluPhe: 1.446 ± 0.028	4.525GluGly: 4.525 ± 0.045	1.375GluHis: 1.375 ± 0.027	2.291GluIle: 2.291 ± 0.034	1.444GluLys: 1.444 ± 0.034	6.794GluLeu: 6.794 ± 0.064	0.836GluMet: 0.836 ± 0.02	0.997GluAsn: 0.997 ± 0.024	3.43GluPro: 3.43 ± 0.046	2.149GluGln: 2.149 ± 0.04	5.589GluArg: 5.589 ± 0.053	2.538GluSer: 2.538 ± 0.037	2.92GluThr: 2.92 ± 0.04	4.462GluVal: 4.462 ± 0.05	0.746GluTrp: 0.746 ± 0.016	1.08GluTyr: 1.08 ± 0.024	0.007GluXaa: 0.007 ± 0.002
Phe	3.767PheAla: 3.767 ± 0.046	0.273PheCys: 0.273 ± 0.012	1.927PheAsp: 1.927 ± 0.034	1.421PheGlu: 1.421 ± 0.029	0.868PhePhe: 0.868 ± 0.023	3.044PheGly: 3.044 ± 0.036	0.635PheHis: 0.635 ± 0.018	0.641PheIle: 0.641 ± 0.018	0.488PheLys: 0.488 ± 0.018	2.622PheLeu: 2.622 ± 0.037	0.389PheMet: 0.389 ± 0.014	0.529PheAsn: 0.529 ± 0.015	1.374PhePro: 1.374 ± 0.025	0.647PheGln: 0.647 ± 0.019	1.903PheArg: 1.903 ± 0.034	1.423PheSer: 1.423 ± 0.027	1.998PheThr: 1.998 ± 0.035	2.268PheVal: 2.268 ± 0.027	0.424PheTrp: 0.424 ± 0.015	0.568PheTyr: 0.568 ± 0.016	0.001PheXaa: 0.001 ± 0.001
Gly	11.584GlyAla: 11.584 ± 0.095	0.857GlyCys: 0.857 ± 0.022	5.257GlyAsp: 5.257 ± 0.047	5.161GlyGlu: 5.161 ± 0.046	2.955GlyPhe: 2.955 ± 0.047	9.191GlyGly: 9.191 ± 0.097	2.317GlyHis: 2.317 ± 0.032	3.425GlyIle: 3.425 ± 0.047	2.348GlyLys: 2.348 ± 0.036	9.8GlyLeu: 9.8 ± 0.079	1.972GlyMet: 1.972 ± 0.031	1.732GlyAsn: 1.732 ± 0.033	5.495GlyPro: 5.495 ± 0.068	2.51GlyGln: 2.51 ± 0.036	8.304GlyArg: 8.304 ± 0.07	5.435GlySer: 5.435 ± 0.058	6.614GlyThr: 6.614 ± 0.063	7.634GlyVal: 7.634 ± 0.064	1.746GlyTrp: 1.746 ± 0.028	2.256GlyTyr: 2.256 ± 0.04	0.018GlyXaa: 0.018 ± 0.003
His	2.732HisAla: 2.732 ± 0.038	0.195HisCys: 0.195 ± 0.01	1.306HisAsp: 1.306 ± 0.028	1.224HisGlu: 1.224 ± 0.023	0.624HisPhe: 0.624 ± 0.017	2.405HisGly: 2.405 ± 0.036	0.671HisHis: 0.671 ± 0.018	0.608HisIle: 0.608 ± 0.017	0.325HisLys: 0.325 ± 0.013	2.366HisLeu: 2.366 ± 0.036	0.322HisMet: 0.322 ± 0.011	0.334HisAsn: 0.334 ± 0.013	1.756HisPro: 1.756 ± 0.029	0.607HisGln: 0.607 ± 0.018	2.14HisArg: 2.14 ± 0.036	0.942HisSer: 0.942 ± 0.021	1.294HisThr: 1.294 ± 0.024	1.711HisVal: 1.711 ± 0.024	0.355HisTrp: 0.355 ± 0.013	0.478HisTyr: 0.478 ± 0.016	0.002HisXaa: 0.002 ± 0.001
Ile	4.662IleAla: 4.662 ± 0.05	0.28IleCys: 0.28 ± 0.013	2.148IleAsp: 2.148 ± 0.037	1.908IleGlu: 1.908 ± 0.03	0.662IlePhe: 0.662 ± 0.018	3.506IleGly: 3.506 ± 0.047	0.588IleHis: 0.588 ± 0.018	0.789IleIle: 0.789 ± 0.018	0.693IleLys: 0.693 ± 0.019	2.392IleLeu: 2.392 ± 0.037	0.416IleMet: 0.416 ± 0.016	0.64IleAsn: 0.64 ± 0.018	1.738IlePro: 1.738 ± 0.029	0.708IleGln: 0.708 ± 0.019	2.217IleArg: 2.217 ± 0.033	1.525IleSer: 1.525 ± 0.028	2.104IleThr: 2.104 ± 0.032	2.763IleVal: 2.763 ± 0.041	0.359IleTrp: 0.359 ± 0.013	0.493IleTyr: 0.493 ± 0.014	0.001IleXaa: 0.001 ± 0.001
Lys	2.919LysAla: 2.919 ± 0.049	0.11LysCys: 0.11 ± 0.008	1.315LysAsp: 1.315 ± 0.032	1.152LysGlu: 1.152 ± 0.024	0.439LysPhe: 0.439 ± 0.014	1.81LysGly: 1.81 ± 0.034	0.401LysHis: 0.401 ± 0.017	0.882LysIle: 0.882 ± 0.024	0.835LysLys: 0.835 ± 0.031	1.917LysLeu: 1.917 ± 0.031	0.359LysMet: 0.359 ± 0.014	0.512LysAsn: 0.512 ± 0.018	1.295LysPro: 1.295 ± 0.032	0.665LysGln: 0.665 ± 0.019	1.353LysArg: 1.353 ± 0.029	1.04LysSer: 1.04 ± 0.019	1.255LysThr: 1.255 ± 0.03	1.824LysVal: 1.824 ± 0.036	0.25LysTrp: 0.25 ± 0.011	0.422LysTyr: 0.422 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	15.507LeuAla: 15.507 ± 0.122	0.841LeuCys: 0.841 ± 0.02	6.83LeuAsp: 6.83 ± 0.063	4.662LeuGlu: 4.662 ± 0.055	2.571LeuPhe: 2.571 ± 0.041	9.558LeuGly: 9.558 ± 0.077	2.287LeuHis: 2.287 ± 0.036	3.093LeuIle: 3.093 ± 0.048	1.958LeuLys: 1.958 ± 0.036	11.583LeuLeu: 11.583 ± 0.108	1.639LeuMet: 1.639 ± 0.028	1.633LeuAsn: 1.633 ± 0.032	6.547LeuPro: 6.547 ± 0.061	2.109LeuGln: 2.109 ± 0.032	8.761LeuArg: 8.761 ± 0.069	5.153LeuSer: 5.153 ± 0.044	6.864LeuThr: 6.864 ± 0.068	9.148LeuVal: 9.148 ± 0.093	1.299LeuTrp: 1.299 ± 0.023	1.8LeuTyr: 1.8 ± 0.026	0.016LeuXaa: 0.016 ± 0.003
Met	2.223MetAla: 2.223 ± 0.035	0.145MetCys: 0.145 ± 0.009	0.875MetAsp: 0.875 ± 0.021	0.743MetGlu: 0.743 ± 0.021	0.438MetPhe: 0.438 ± 0.015	1.3MetGly: 1.3 ± 0.026	0.324MetHis: 0.324 ± 0.013	0.662MetIle: 0.662 ± 0.019	0.436MetLys: 0.436 ± 0.016	1.655MetLeu: 1.655 ± 0.029	0.279MetMet: 0.279 ± 0.011	0.446MetAsn: 0.446 ± 0.014	1.074MetPro: 1.074 ± 0.023	0.438MetGln: 0.438 ± 0.014	1.418MetArg: 1.418 ± 0.025	1.29MetSer: 1.29 ± 0.025	1.577MetThr: 1.577 ± 0.027	1.23MetVal: 1.23 ± 0.025	0.218MetTrp: 0.218 ± 0.009	0.307MetTyr: 0.307 ± 0.013	0.002MetXaa: 0.002 ± 0.001
Asn	2.141AsnAla: 2.141 ± 0.038	0.161AsnCys: 0.161 ± 0.009	0.93AsnAsp: 0.93 ± 0.022	0.787AsnGlu: 0.787 ± 0.021	0.458AsnPhe: 0.458 ± 0.015	1.847AsnGly: 1.847 ± 0.032	0.407AsnHis: 0.407 ± 0.013	0.622AsnIle: 0.622 ± 0.021	0.387AsnLys: 0.387 ± 0.015	1.599AsnLeu: 1.599 ± 0.031	0.294AsnMet: 0.294 ± 0.014	0.406AsnAsn: 0.406 ± 0.018	1.298AsnPro: 1.298 ± 0.03	0.468AsnGln: 0.468 ± 0.016	1.212AsnArg: 1.212 ± 0.022	0.864AsnSer: 0.864 ± 0.024	1.055AsnThr: 1.055 ± 0.024	1.337AsnVal: 1.337 ± 0.027	0.271AsnTrp: 0.271 ± 0.01	0.37AsnTyr: 0.37 ± 0.014	0.001AsnXaa: 0.001 ± 0.001
Pro	9.039ProAla: 9.039 ± 0.09	0.335ProCys: 0.335 ± 0.014	4.452ProAsp: 4.452 ± 0.051	4.446ProGlu: 4.446 ± 0.056	1.553ProPhe: 1.553 ± 0.027	6.848ProGly: 6.848 ± 0.071	1.332ProHis: 1.332 ± 0.028	1.23ProIle: 1.23 ± 0.026	1.108ProLys: 1.108 ± 0.028	5.474ProLeu: 5.474 ± 0.051	1.007ProMet: 1.007 ± 0.021	0.836ProAsn: 0.836 ± 0.02	3.446ProPro: 3.446 ± 0.056	1.643ProGln: 1.643 ± 0.031	4.173ProArg: 4.173 ± 0.051	3.092ProSer: 3.092 ± 0.04	3.047ProThr: 3.047 ± 0.051	5.695ProVal: 5.695 ± 0.054	0.939ProTrp: 0.939 ± 0.019	1.412ProTyr: 1.412 ± 0.029	0.013ProXaa: 0.013 ± 0.002
Gln	3.516GlnAla: 3.516 ± 0.04	0.158GlnCys: 0.158 ± 0.008	1.344GlnAsp: 1.344 ± 0.027	1.416GlnGlu: 1.416 ± 0.026	0.648GlnPhe: 0.648 ± 0.019	2.292GlnGly: 2.292 ± 0.034	0.59GlnHis: 0.59 ± 0.017	1.048GlnIle: 1.048 ± 0.023	0.603GlnLys: 0.603 ± 0.018	2.764GlnLeu: 2.764 ± 0.04	0.446GlnMet: 0.446 ± 0.015	0.477GlnAsn: 0.477 ± 0.016	1.565GlnPro: 1.565 ± 0.034	1.167GlnGln: 1.167 ± 0.041	2.171GlnArg: 2.171 ± 0.034	1.171GlnSer: 1.171 ± 0.025	1.267GlnThr: 1.267 ± 0.028	2.269GlnVal: 2.269 ± 0.034	0.409GlnTrp: 0.409 ± 0.015	0.582GlnTyr: 0.582 ± 0.02	0.003GlnXaa: 0.003 ± 0.001
Arg	10.218ArgAla: 10.218 ± 0.076	0.621ArgCys: 0.621 ± 0.018	4.312ArgAsp: 4.312 ± 0.053	4.763ArgGlu: 4.763 ± 0.058	2.355ArgPhe: 2.355 ± 0.029	6.054ArgGly: 6.054 ± 0.055	2.156ArgHis: 2.156 ± 0.034	3.399ArgIle: 3.399 ± 0.039	1.615ArgLys: 1.615 ± 0.032	8.993ArgLeu: 8.993 ± 0.077	1.744ArgMet: 1.744 ± 0.028	1.356ArgAsn: 1.356 ± 0.026	5.193ArgPro: 5.193 ± 0.054	2.155ArgGln: 2.155 ± 0.034	8.018ArgArg: 8.018 ± 0.088	4.11ArgSer: 4.11 ± 0.053	5.703ArgThr: 5.703 ± 0.048	5.912ArgVal: 5.912 ± 0.064	1.391ArgTrp: 1.391 ± 0.025	1.805ArgTyr: 1.805 ± 0.033	0.014ArgXaa: 0.014 ± 0.003
Ser	6.556SerAla: 6.556 ± 0.063	0.425SerCys: 0.425 ± 0.016	2.54SerAsp: 2.54 ± 0.044	2.347SerGlu: 2.347 ± 0.037	1.529SerPhe: 1.529 ± 0.027	5.864SerGly: 5.864 ± 0.056	0.956SerHis: 0.956 ± 0.023	1.382SerIle: 1.382 ± 0.026	0.943SerLys: 0.943 ± 0.022	4.891SerLeu: 4.891 ± 0.06	1.088SerMet: 1.088 ± 0.02	0.784SerAsn: 0.784 ± 0.023	3.048SerPro: 3.048 ± 0.043	1.123SerGln: 1.123 ± 0.021	3.691SerArg: 3.691 ± 0.044	2.662SerSer: 2.662 ± 0.045	2.963SerThr: 2.963 ± 0.044	4.296SerVal: 4.296 ± 0.044	0.899SerTrp: 0.899 ± 0.019	1.195SerTyr: 1.195 ± 0.024	0.005SerXaa: 0.005 ± 0.002
Thr	9.111ThrAla: 9.111 ± 0.065	0.437ThrCys: 0.437 ± 0.015	3.625ThrAsp: 3.625 ± 0.047	3.306ThrGlu: 3.306 ± 0.045	1.616ThrPhe: 1.616 ± 0.029	6.924ThrGly: 6.924 ± 0.07	1.166ThrHis: 1.166 ± 0.025	1.683ThrIle: 1.683 ± 0.032	1.11ThrLys: 1.11 ± 0.028	5.834ThrLeu: 5.834 ± 0.065	0.91ThrMet: 0.91 ± 0.021	0.96ThrAsn: 0.96 ± 0.024	4.114ThrPro: 4.114 ± 0.046	1.265ThrGln: 1.265 ± 0.03	4.022ThrArg: 4.022 ± 0.05	3.094ThrSer: 3.094 ± 0.049	3.885ThrThr: 3.885 ± 0.046	6.236ThrVal: 6.236 ± 0.061	0.906ThrTrp: 0.906 ± 0.023	1.326ThrTyr: 1.326 ± 0.03	0.008ThrXaa: 0.008 ± 0.002
Val	11.075ValAla: 11.075 ± 0.073	0.755ValCys: 0.755 ± 0.02	5.14ValAsp: 5.14 ± 0.053	4.888ValGlu: 4.888 ± 0.062	2.428ValPhe: 2.428 ± 0.038	6.533ValGly: 6.533 ± 0.066	2.001ValHis: 2.001 ± 0.032	2.582ValIle: 2.582 ± 0.044	1.682ValLys: 1.682 ± 0.031	9.77ValLeu: 9.77 ± 0.085	1.436ValMet: 1.436 ± 0.027	1.633ValAsn: 1.633 ± 0.034	5.433ValPro: 5.433 ± 0.058	1.983ValGln: 1.983 ± 0.033	7.284ValArg: 7.284 ± 0.059	4.264ValSer: 4.264 ± 0.045	5.737ValThr: 5.737 ± 0.058	8.317ValVal: 8.317 ± 0.08	1.178ValTrp: 1.178 ± 0.024	1.581ValTyr: 1.581 ± 0.027	0.012ValXaa: 0.012 ± 0.002
Trp	1.656TrpAla: 1.656 ± 0.029	0.158TrpCys: 0.158 ± 0.009	0.834TrpAsp: 0.834 ± 0.024	0.746TrpGlu: 0.746 ± 0.02	0.512TrpPhe: 0.512 ± 0.016	1.064TrpGly: 1.064 ± 0.025	0.337TrpHis: 0.337 ± 0.012	0.598TrpIle: 0.598 ± 0.017	0.371TrpLys: 0.371 ± 0.013	1.732TrpLeu: 1.732 ± 0.032	0.264TrpMet: 0.264 ± 0.01	0.399TrpAsn: 0.399 ± 0.016	0.781TrpPro: 0.781 ± 0.019	0.588TrpGln: 0.588 ± 0.018	1.33TrpArg: 1.33 ± 0.024	0.969TrpSer: 0.969 ± 0.02	1.134TrpThr: 1.134 ± 0.024	0.978TrpVal: 0.978 ± 0.02	0.345TrpTrp: 0.345 ± 0.012	0.358TrpTyr: 0.358 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.763TyrAla: 2.763 ± 0.042	0.168TyrCys: 0.168 ± 0.008	1.471TyrAsp: 1.471 ± 0.028	1.303TyrGlu: 1.303 ± 0.026	0.645TyrPhe: 0.645 ± 0.019	2.306TyrGly: 2.306 ± 0.038	0.388TyrHis: 0.388 ± 0.014	0.434TyrIle: 0.434 ± 0.015	0.388TyrLys: 0.388 ± 0.013	1.996TyrLeu: 1.996 ± 0.033	0.258TyrMet: 0.258 ± 0.01	0.365TyrAsn: 0.365 ± 0.015	1.039TyrPro: 1.039 ± 0.024	0.58TyrGln: 0.58 ± 0.018	1.838TyrArg: 1.838 ± 0.031	0.895TyrSer: 0.895 ± 0.024	1.212TyrThr: 1.212 ± 0.022	1.658TyrVal: 1.658 ± 0.027	0.332TyrTrp: 0.332 ± 0.013	0.438TyrTyr: 0.438 ± 0.016	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.031XaaAla: 0.031 ± 0.004	0.001XaaCys: 0.001 ± 0.001	0.009XaaAsp: 0.009 ± 0.002	0.009XaaGlu: 0.009 ± 0.002	0.003XaaPhe: 0.003 ± 0.001	0.02XaaGly: 0.02 ± 0.003	0.002XaaHis: 0.002 ± 0.001	0.004XaaIle: 0.004 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.012XaaLeu: 0.012 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.003XaaAsn: 0.003 ± 0.001	0.007XaaPro: 0.007 ± 0.002	0.002XaaGln: 0.002 ± 0.001	0.014XaaArg: 0.014 ± 0.003	0.007XaaSer: 0.007 ± 0.002	0.009XaaThr: 0.009 ± 0.002	0.011XaaVal: 0.011 ± 0.002	0.002XaaTrp: 0.002 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.006XaaXaa: 0.006 ± 0.002

Statistics based on 6788 proteins (2132704 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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