Escherichia phage PGN6866

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kuravirus; unclassified Kuravirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9E8E9|A0A6M9E8E9_9CAUD Uncharacterized protein OS=Escherichia phage PGN6866 OX=2714736 PE=4 SV=1
MM1 pKa = 7.47GLFGGGGSKK10 pKa = 7.37TTSQMIRR17 pKa = 11.84PEE19 pKa = 4.35YY20 pKa = 7.84IQQYY24 pKa = 10.06IDD26 pKa = 3.97QLNQQIGNTSSGDD39 pKa = 3.61YY40 pKa = 10.33VYY42 pKa = 10.99HH43 pKa = 7.61DD44 pKa = 3.17NVGFNNNQTEE54 pKa = 4.16ALNNLAQSGALGTLSQQYY72 pKa = 9.36MDD74 pKa = 4.46AAGQGLGYY82 pKa = 10.18LDD84 pKa = 3.91NAYY87 pKa = 9.77KK88 pKa = 10.68GYY90 pKa = 10.55QSLSGQGGITGEE102 pKa = 4.22QIGALAGQLYY112 pKa = 10.8DD113 pKa = 4.67DD114 pKa = 5.52DD115 pKa = 5.07AVQAAITANNEE126 pKa = 3.74QVQQNLARR134 pKa = 11.84NALPQLAQQFAGQQGSGARR153 pKa = 11.84MAKK156 pKa = 10.07SFAQGDD162 pKa = 4.43ALNQMQGQATDD173 pKa = 3.61ITNSAYY179 pKa = 10.88NSAISQAEE187 pKa = 4.25SILSGNRR194 pKa = 11.84QNQAAALSGLSNIGSNLSNLGQQGANLSQQQIMNQWNAGLQQQQQQQNEE243 pKa = 4.18YY244 pKa = 11.28DD245 pKa = 3.32NAYY248 pKa = 10.94QNAQNAANWGWQDD261 pKa = 3.36INNQLGAAGVLNGALGQTTTTKK283 pKa = 10.39TSGGKK288 pKa = 10.3GGFLGGAMSGAAAGSSFGPWGALAGGVIGGLASTT322 pKa = 4.88

Molecular weight: 33.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9EFC1|A0A6M9EFC1_9CAUD Uncharacterized protein OS=Escherichia phage PGN6866 OX=2714736 PE=4 SV=1
MM1 pKa = 7.38FSGSHH6 pKa = 6.59RR7 pKa = 11.84LLKK10 pKa = 10.6KK11 pKa = 10.35CITVIHH17 pKa = 6.96IGTSKK22 pKa = 10.55CFSILSRR29 pKa = 11.84YY30 pKa = 9.86ALIFHH35 pKa = 6.29STHH38 pKa = 4.7QAEE41 pKa = 4.57MNVITLTLYY50 pKa = 8.71CHH52 pKa = 6.6KK53 pKa = 10.91VILTLLGRR61 pKa = 11.84FKK63 pKa = 11.26LFTALNSLVHH73 pKa = 6.37IKK75 pKa = 9.54LTVHH79 pKa = 6.5FFRR82 pKa = 11.84LLIHH86 pKa = 6.53LVPLGLTSVFLCISS100 pKa = 3.48

Molecular weight: 11.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

11733

100

1005

286.2

31.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.233 ± 0.472

0.955 ± 0.148

6.256 ± 0.34

6.085 ± 0.521

3.716 ± 0.187

8.088 ± 0.674

1.568 ± 0.224

5.651 ± 0.177

6.017 ± 0.405

7.108 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.244

5.872 ± 0.37

3.946 ± 0.263

4.918 ± 0.495

4.73 ± 0.315

6.844 ± 0.407

6.051 ± 0.326

6.656 ± 0.381

1.372 ± 0.113

3.392 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski