Amino acid dipepetide frequency for Escherichia phage PGN6866

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.098AlaAla: 8.098 ± 1.241
0.682AlaCys: 0.682 ± 0.274
5.967AlaAsp: 5.967 ± 0.732
4.347AlaGlu: 4.347 ± 0.822
2.472AlaPhe: 2.472 ± 0.416
6.308AlaGly: 6.308 ± 0.842
1.023AlaHis: 1.023 ± 0.334
4.091AlaIle: 4.091 ± 0.532
4.603AlaLys: 4.603 ± 1.045
7.842AlaLeu: 7.842 ± 0.763
2.046AlaMet: 2.046 ± 0.446
4.688AlaAsn: 4.688 ± 0.551
2.728AlaPro: 2.728 ± 0.55
4.773AlaGln: 4.773 ± 1.004
4.091AlaArg: 4.091 ± 0.568
5.711AlaSer: 5.711 ± 0.941
4.859AlaThr: 4.859 ± 0.739
4.347AlaVal: 4.347 ± 0.75
1.023AlaTrp: 1.023 ± 0.336
2.642AlaTyr: 2.642 ± 0.495
0.0AlaXaa: 0.0 ± 0.0
Cys
1.023CysAla: 1.023 ± 0.36
0.085CysCys: 0.085 ± 0.095
0.17CysAsp: 0.17 ± 0.129
0.341CysGlu: 0.341 ± 0.176
0.426CysPhe: 0.426 ± 0.195
0.852CysGly: 0.852 ± 0.385
0.511CysHis: 0.511 ± 0.178
0.682CysIle: 0.682 ± 0.241
0.256CysLys: 0.256 ± 0.147
0.597CysLeu: 0.597 ± 0.22
0.0CysMet: 0.0 ± 0.0
0.597CysAsn: 0.597 ± 0.246
0.256CysPro: 0.256 ± 0.157
0.426CysGln: 0.426 ± 0.183
0.682CysArg: 0.682 ± 0.252
0.682CysSer: 0.682 ± 0.215
0.511CysThr: 0.511 ± 0.263
1.108CysVal: 1.108 ± 0.275
0.17CysTrp: 0.17 ± 0.142
0.17CysTyr: 0.17 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
5.967AspAla: 5.967 ± 0.731
0.426AspCys: 0.426 ± 0.157
2.472AspAsp: 2.472 ± 0.52
3.921AspGlu: 3.921 ± 0.82
1.79AspPhe: 1.79 ± 0.413
6.308AspGly: 6.308 ± 1.78
1.108AspHis: 1.108 ± 0.391
4.091AspIle: 4.091 ± 0.603
3.75AspLys: 3.75 ± 0.636
4.518AspLeu: 4.518 ± 0.58
1.875AspMet: 1.875 ± 0.461
3.836AspAsn: 3.836 ± 0.632
2.898AspPro: 2.898 ± 0.576
1.96AspGln: 1.96 ± 0.356
2.472AspArg: 2.472 ± 0.435
3.665AspSer: 3.665 ± 0.548
3.665AspThr: 3.665 ± 0.559
4.347AspVal: 4.347 ± 0.572
0.852AspTrp: 0.852 ± 0.282
2.642AspTyr: 2.642 ± 0.565
0.0AspXaa: 0.0 ± 0.0
Glu
5.199GluAla: 5.199 ± 0.773
0.938GluCys: 0.938 ± 0.32
4.177GluAsp: 4.177 ± 1.063
5.881GluGlu: 5.881 ± 1.4
2.046GluPhe: 2.046 ± 0.462
3.75GluGly: 3.75 ± 0.492
0.938GluHis: 0.938 ± 0.335
4.091GluIle: 4.091 ± 0.591
4.091GluLys: 4.091 ± 0.651
3.921GluLeu: 3.921 ± 0.791
2.046GluMet: 2.046 ± 0.335
2.728GluAsn: 2.728 ± 0.6
1.364GluPro: 1.364 ± 0.408
2.983GluGln: 2.983 ± 0.892
3.495GluArg: 3.495 ± 0.665
3.324GluSer: 3.324 ± 0.674
2.642GluThr: 2.642 ± 0.458
4.006GluVal: 4.006 ± 0.637
0.852GluTrp: 0.852 ± 0.292
2.387GluTyr: 2.387 ± 0.48
0.0GluXaa: 0.0 ± 0.0
Phe
2.728PheAla: 2.728 ± 0.539
0.682PheCys: 0.682 ± 0.3
2.472PheAsp: 2.472 ± 0.552
2.472PheGlu: 2.472 ± 0.435
1.023PhePhe: 1.023 ± 0.285
2.131PheGly: 2.131 ± 0.426
0.852PheHis: 0.852 ± 0.253
2.131PheIle: 2.131 ± 0.446
2.472PheLys: 2.472 ± 0.532
2.557PheLeu: 2.557 ± 0.581
0.511PheMet: 0.511 ± 0.227
3.154PheAsn: 3.154 ± 0.555
1.193PhePro: 1.193 ± 0.283
1.875PheGln: 1.875 ± 0.387
2.472PheArg: 2.472 ± 0.476
2.046PheSer: 2.046 ± 0.506
1.705PheThr: 1.705 ± 0.274
2.131PheVal: 2.131 ± 0.514
0.085PheTrp: 0.085 ± 0.1
2.472PheTyr: 2.472 ± 0.517
0.0PheXaa: 0.0 ± 0.0
Gly
7.416GlyAla: 7.416 ± 1.255
1.023GlyCys: 1.023 ± 0.385
4.091GlyAsp: 4.091 ± 0.877
4.262GlyGlu: 4.262 ± 0.86
3.324GlyPhe: 3.324 ± 0.401
7.16GlyGly: 7.16 ± 1.361
1.023GlyHis: 1.023 ± 0.284
5.199GlyIle: 5.199 ± 1.135
5.285GlyLys: 5.285 ± 1.132
6.648GlyLeu: 6.648 ± 1.049
2.046GlyMet: 2.046 ± 0.499
3.75GlyAsn: 3.75 ± 0.581
1.279GlyPro: 1.279 ± 0.315
2.898GlyGln: 2.898 ± 0.778
4.006GlyArg: 4.006 ± 0.743
8.438GlySer: 8.438 ± 1.142
4.091GlyThr: 4.091 ± 0.709
5.796GlyVal: 5.796 ± 0.956
1.023GlyTrp: 1.023 ± 0.304
2.131GlyTyr: 2.131 ± 0.426
0.0GlyXaa: 0.0 ± 0.0
His
1.023HisAla: 1.023 ± 0.337
0.085HisCys: 0.085 ± 0.065
0.852HisAsp: 0.852 ± 0.323
1.023HisGlu: 1.023 ± 0.294
0.767HisPhe: 0.767 ± 0.227
0.682HisGly: 0.682 ± 0.275
0.17HisHis: 0.17 ± 0.128
1.108HisIle: 1.108 ± 0.281
1.279HisLys: 1.279 ± 0.365
1.193HisLeu: 1.193 ± 0.319
0.341HisMet: 0.341 ± 0.173
1.108HisAsn: 1.108 ± 0.35
0.426HisPro: 0.426 ± 0.193
0.511HisGln: 0.511 ± 0.178
1.108HisArg: 1.108 ± 0.386
0.597HisSer: 0.597 ± 0.229
1.534HisThr: 1.534 ± 0.315
0.852HisVal: 0.852 ± 0.298
0.256HisTrp: 0.256 ± 0.178
0.767HisTyr: 0.767 ± 0.292
0.0HisXaa: 0.0 ± 0.0
Ile
3.409IleAla: 3.409 ± 0.6
0.426IleCys: 0.426 ± 0.196
4.432IleAsp: 4.432 ± 0.885
3.495IleGlu: 3.495 ± 0.436
1.875IlePhe: 1.875 ± 0.425
4.347IleGly: 4.347 ± 0.826
0.938IleHis: 0.938 ± 0.305
1.79IleIle: 1.79 ± 0.48
4.262IleLys: 4.262 ± 0.511
3.324IleLeu: 3.324 ± 0.541
1.534IleMet: 1.534 ± 0.397
3.665IleAsn: 3.665 ± 0.601
4.262IlePro: 4.262 ± 0.736
2.046IleGln: 2.046 ± 0.541
2.728IleArg: 2.728 ± 0.456
4.006IleSer: 4.006 ± 0.562
4.177IleThr: 4.177 ± 0.593
3.069IleVal: 3.069 ± 0.514
1.023IleTrp: 1.023 ± 0.327
1.705IleTyr: 1.705 ± 0.362
0.0IleXaa: 0.0 ± 0.0
Lys
5.199LysAla: 5.199 ± 0.656
0.511LysCys: 0.511 ± 0.197
5.54LysAsp: 5.54 ± 0.949
5.029LysGlu: 5.029 ± 0.852
2.046LysPhe: 2.046 ± 0.429
4.347LysGly: 4.347 ± 0.8
1.534LysHis: 1.534 ± 0.418
2.046LysIle: 2.046 ± 0.432
4.432LysLys: 4.432 ± 0.83
4.006LysLeu: 4.006 ± 0.571
1.364LysMet: 1.364 ± 0.321
2.983LysAsn: 2.983 ± 0.501
1.79LysPro: 1.79 ± 0.487
2.983LysGln: 2.983 ± 0.468
2.983LysArg: 2.983 ± 0.751
3.324LysSer: 3.324 ± 0.629
3.836LysThr: 3.836 ± 0.68
3.409LysVal: 3.409 ± 0.527
0.852LysTrp: 0.852 ± 0.337
1.96LysTyr: 1.96 ± 0.395
0.0LysXaa: 0.0 ± 0.0
Leu
6.137LeuAla: 6.137 ± 0.884
1.279LeuCys: 1.279 ± 0.336
3.836LeuAsp: 3.836 ± 0.476
4.859LeuGlu: 4.859 ± 0.814
2.642LeuPhe: 2.642 ± 0.421
6.308LeuGly: 6.308 ± 1.152
0.852LeuHis: 0.852 ± 0.253
3.154LeuIle: 3.154 ± 0.641
3.75LeuLys: 3.75 ± 0.594
5.967LeuLeu: 5.967 ± 0.811
2.472LeuMet: 2.472 ± 0.39
4.091LeuAsn: 4.091 ± 0.812
2.813LeuPro: 2.813 ± 0.434
3.921LeuGln: 3.921 ± 0.693
3.495LeuArg: 3.495 ± 0.599
4.944LeuSer: 4.944 ± 0.678
4.177LeuThr: 4.177 ± 0.701
4.091LeuVal: 4.091 ± 0.613
0.426LeuTrp: 0.426 ± 0.18
1.875LeuTyr: 1.875 ± 0.277
0.0LeuXaa: 0.0 ± 0.0
Met
2.301MetAla: 2.301 ± 0.475
0.17MetCys: 0.17 ± 0.137
1.193MetAsp: 1.193 ± 0.338
1.705MetGlu: 1.705 ± 0.43
1.279MetPhe: 1.279 ± 0.308
1.62MetGly: 1.62 ± 0.495
0.426MetHis: 0.426 ± 0.234
1.705MetIle: 1.705 ± 0.371
2.387MetLys: 2.387 ± 0.55
1.193MetLeu: 1.193 ± 0.32
0.682MetMet: 0.682 ± 0.228
1.449MetAsn: 1.449 ± 0.361
1.108MetPro: 1.108 ± 0.246
1.023MetGln: 1.023 ± 0.282
1.534MetArg: 1.534 ± 0.425
1.705MetSer: 1.705 ± 0.517
1.534MetThr: 1.534 ± 0.343
1.449MetVal: 1.449 ± 0.391
0.341MetTrp: 0.341 ± 0.203
0.597MetTyr: 0.597 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
5.285AsnAla: 5.285 ± 0.814
0.256AsnCys: 0.256 ± 0.124
2.642AsnAsp: 2.642 ± 0.451
2.046AsnGlu: 2.046 ± 0.431
2.387AsnPhe: 2.387 ± 0.567
5.711AsnGly: 5.711 ± 0.741
0.341AsnHis: 0.341 ± 0.142
3.58AsnIle: 3.58 ± 0.429
2.472AsnLys: 2.472 ± 0.399
4.688AsnLeu: 4.688 ± 0.769
1.449AsnMet: 1.449 ± 0.394
3.75AsnAsn: 3.75 ± 0.695
3.324AsnPro: 3.324 ± 0.604
3.665AsnGln: 3.665 ± 0.659
2.642AsnArg: 2.642 ± 0.386
3.921AsnSer: 3.921 ± 0.649
3.495AsnThr: 3.495 ± 0.646
4.432AsnVal: 4.432 ± 0.618
1.364AsnTrp: 1.364 ± 0.357
1.279AsnTyr: 1.279 ± 0.262
0.0AsnXaa: 0.0 ± 0.0
Pro
3.154ProAla: 3.154 ± 0.476
0.256ProCys: 0.256 ± 0.148
2.557ProAsp: 2.557 ± 0.648
2.472ProGlu: 2.472 ± 0.425
1.875ProPhe: 1.875 ± 0.312
2.387ProGly: 2.387 ± 0.529
0.597ProHis: 0.597 ± 0.218
2.301ProIle: 2.301 ± 0.347
1.79ProLys: 1.79 ± 0.338
3.239ProLeu: 3.239 ± 0.611
0.597ProMet: 0.597 ± 0.262
2.131ProAsn: 2.131 ± 0.42
2.387ProPro: 2.387 ± 0.482
1.449ProGln: 1.449 ± 0.387
1.023ProArg: 1.023 ± 0.252
2.813ProSer: 2.813 ± 0.554
2.813ProThr: 2.813 ± 0.641
3.665ProVal: 3.665 ± 0.604
0.767ProTrp: 0.767 ± 0.222
1.193ProTyr: 1.193 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
4.603GlnAla: 4.603 ± 0.763
0.682GlnCys: 0.682 ± 0.281
3.069GlnAsp: 3.069 ± 0.74
2.387GlnGlu: 2.387 ± 0.538
1.449GlnPhe: 1.449 ± 0.282
4.091GlnGly: 4.091 ± 1.151
0.256GlnHis: 0.256 ± 0.139
3.239GlnIle: 3.239 ± 0.495
3.069GlnLys: 3.069 ± 0.537
3.239GlnLeu: 3.239 ± 0.646
1.193GlnMet: 1.193 ± 0.337
3.495GlnAsn: 3.495 ± 0.844
1.449GlnPro: 1.449 ± 0.299
4.518GlnGln: 4.518 ± 1.767
2.301GlnArg: 2.301 ± 0.418
2.131GlnSer: 2.131 ± 0.446
2.898GlnThr: 2.898 ± 0.515
2.301GlnVal: 2.301 ± 0.592
0.767GlnTrp: 0.767 ± 0.262
1.96GlnTyr: 1.96 ± 0.389
0.0GlnXaa: 0.0 ± 0.0
Arg
3.75ArgAla: 3.75 ± 0.647
0.597ArgCys: 0.597 ± 0.275
2.983ArgAsp: 2.983 ± 0.602
2.472ArgGlu: 2.472 ± 0.548
2.301ArgPhe: 2.301 ± 0.445
2.898ArgGly: 2.898 ± 0.607
0.682ArgHis: 0.682 ± 0.242
2.472ArgIle: 2.472 ± 0.616
2.728ArgLys: 2.728 ± 0.555
2.813ArgLeu: 2.813 ± 0.515
1.193ArgMet: 1.193 ± 0.243
2.216ArgAsn: 2.216 ± 0.417
1.96ArgPro: 1.96 ± 0.337
2.131ArgGln: 2.131 ± 0.458
2.557ArgArg: 2.557 ± 0.484
3.75ArgSer: 3.75 ± 0.553
2.983ArgThr: 2.983 ± 0.53
4.347ArgVal: 4.347 ± 0.566
0.682ArgTrp: 0.682 ± 0.205
1.79ArgTyr: 1.79 ± 0.451
0.0ArgXaa: 0.0 ± 0.0
Ser
3.58SerAla: 3.58 ± 0.506
0.426SerCys: 0.426 ± 0.166
4.432SerAsp: 4.432 ± 0.79
3.409SerGlu: 3.409 ± 0.462
2.983SerPhe: 2.983 ± 0.508
7.586SerGly: 7.586 ± 1.259
1.023SerHis: 1.023 ± 0.271
4.177SerIle: 4.177 ± 0.62
4.006SerLys: 4.006 ± 0.559
4.603SerLeu: 4.603 ± 0.536
2.046SerMet: 2.046 ± 0.413
4.432SerAsn: 4.432 ± 0.846
3.069SerPro: 3.069 ± 0.53
2.813SerGln: 2.813 ± 0.57
2.813SerArg: 2.813 ± 0.437
4.518SerSer: 4.518 ± 0.555
4.006SerThr: 4.006 ± 0.652
4.773SerVal: 4.773 ± 0.807
1.193SerTrp: 1.193 ± 0.322
2.557SerTyr: 2.557 ± 0.626
0.0SerXaa: 0.0 ± 0.0
Thr
4.432ThrAla: 4.432 ± 0.668
0.256ThrCys: 0.256 ± 0.132
2.983ThrAsp: 2.983 ± 0.475
3.836ThrGlu: 3.836 ± 0.566
1.875ThrPhe: 1.875 ± 0.453
5.54ThrGly: 5.54 ± 1.057
1.193ThrHis: 1.193 ± 0.369
3.836ThrIle: 3.836 ± 0.789
3.239ThrLys: 3.239 ± 0.477
4.603ThrLeu: 4.603 ± 0.591
1.108ThrMet: 1.108 ± 0.28
3.154ThrAsn: 3.154 ± 0.572
3.154ThrPro: 3.154 ± 0.436
2.983ThrGln: 2.983 ± 0.553
2.046ThrArg: 2.046 ± 0.36
4.091ThrSer: 4.091 ± 0.652
4.006ThrThr: 4.006 ± 0.785
5.199ThrVal: 5.199 ± 0.816
0.938ThrTrp: 0.938 ± 0.341
2.046ThrTyr: 2.046 ± 0.454
0.0ThrXaa: 0.0 ± 0.0
Val
5.199ValAla: 5.199 ± 0.506
0.511ValCys: 0.511 ± 0.218
4.688ValAsp: 4.688 ± 0.659
3.75ValGlu: 3.75 ± 0.485
2.983ValPhe: 2.983 ± 0.723
5.54ValGly: 5.54 ± 0.618
1.023ValHis: 1.023 ± 0.295
3.75ValIle: 3.75 ± 0.661
3.75ValLys: 3.75 ± 0.497
3.75ValLeu: 3.75 ± 0.543
1.108ValMet: 1.108 ± 0.3
4.688ValAsn: 4.688 ± 0.569
2.131ValPro: 2.131 ± 0.59
2.983ValGln: 2.983 ± 0.651
2.557ValArg: 2.557 ± 0.369
5.199ValSer: 5.199 ± 1.172
5.199ValThr: 5.199 ± 1.188
4.688ValVal: 4.688 ± 0.945
0.767ValTrp: 0.767 ± 0.288
2.301ValTyr: 2.301 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
0.938TrpAla: 0.938 ± 0.269
0.0TrpCys: 0.0 ± 0.0
1.279TrpAsp: 1.279 ± 0.284
0.597TrpGlu: 0.597 ± 0.218
0.341TrpPhe: 0.341 ± 0.184
0.938TrpGly: 0.938 ± 0.365
0.256TrpHis: 0.256 ± 0.184
0.767TrpIle: 0.767 ± 0.254
0.597TrpLys: 0.597 ± 0.193
0.511TrpLeu: 0.511 ± 0.206
0.852TrpMet: 0.852 ± 0.316
1.023TrpAsn: 1.023 ± 0.221
0.256TrpPro: 0.256 ± 0.177
0.767TrpGln: 0.767 ± 0.355
1.023TrpArg: 1.023 ± 0.252
1.193TrpSer: 1.193 ± 0.399
1.108TrpThr: 1.108 ± 0.264
0.597TrpVal: 0.597 ± 0.26
0.17TrpTrp: 0.17 ± 0.171
0.511TrpTyr: 0.511 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.898TyrAla: 2.898 ± 0.414
0.256TyrCys: 0.256 ± 0.159
2.728TyrAsp: 2.728 ± 0.516
2.557TyrGlu: 2.557 ± 0.521
1.279TyrPhe: 1.279 ± 0.307
2.387TyrGly: 2.387 ± 0.468
0.938TyrHis: 0.938 ± 0.232
2.301TyrIle: 2.301 ± 0.32
1.96TyrLys: 1.96 ± 0.503
1.875TyrLeu: 1.875 ± 0.384
0.852TyrMet: 0.852 ± 0.262
1.79TyrAsn: 1.79 ± 0.305
1.62TyrPro: 1.62 ± 0.448
2.472TyrGln: 2.472 ± 0.376
1.279TyrArg: 1.279 ± 0.368
2.387TyrSer: 2.387 ± 0.43
1.279TyrThr: 1.279 ± 0.261
1.96TyrVal: 1.96 ± 0.316
0.17TyrTrp: 0.17 ± 0.108
0.938TyrTyr: 0.938 ± 0.347
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (11733 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski