African elephant polyomavirus 1
Average proteome isoelectric point is 7.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T2FFJ0|T2FFJ0_9POLY Minor capsid protein OS=African elephant polyomavirus 1 OX=1399914 GN=AelPyV_gp2 PE=3 SV=1
MM1 pKa = 7.67 APQRR5 pKa = 11.84 KK6 pKa = 9.29 GGGRR10 pKa = 11.84 PCVPSKK16 pKa = 10.88 RR17 pKa = 11.84 PLRR20 pKa = 11.84 KK21 pKa = 9.26 PEE23 pKa = 3.85 NVPRR27 pKa = 11.84 ILVRR31 pKa = 11.84 GGVQVLEE38 pKa = 4.43 VRR40 pKa = 11.84 SGPDD44 pKa = 3.05 SIVDD48 pKa = 3.48 IEE50 pKa = 5.04 CFLQPRR56 pKa = 11.84 MGEE59 pKa = 4.13 PEE61 pKa = 3.99 GDD63 pKa = 3.39 DD64 pKa = 3.49 YY65 pKa = 11.96 YY66 pKa = 11.51 GYY68 pKa = 10.66 SEE70 pKa = 5.98 LITVGTEE77 pKa = 3.54 KK78 pKa = 10.25 STDD81 pKa = 3.36 TPPFKK86 pKa = 10.46 QLPCYY91 pKa = 10.29 SAAQVQLPNLNPDD104 pKa = 4.24 LTCEE108 pKa = 3.95 TLLMWEE114 pKa = 4.21 AVSTKK119 pKa = 10.34 TEE121 pKa = 3.82 VLGISSLVNVHH132 pKa = 5.5 SQSKK136 pKa = 9.0 RR137 pKa = 11.84 VEE139 pKa = 4.14 PEE141 pKa = 4.18 GIGYY145 pKa = 8.45 PIAGINYY152 pKa = 8.5 HH153 pKa = 6.19 FFAVGGQPLDD163 pKa = 3.73 LQALVSNFSATYY175 pKa = 7.56 PAEE178 pKa = 3.98 RR179 pKa = 11.84 KK180 pKa = 10.05 VPVKK184 pKa = 10.78 DD185 pKa = 3.34 QLHH188 pKa = 7.18 PIAQVLDD195 pKa = 4.22 PNLKK199 pKa = 7.71 TTLDD203 pKa = 3.63 EE204 pKa = 5.35 DD205 pKa = 3.54 GTYY208 pKa = 10.3 PIEE211 pKa = 4.2 VWGADD216 pKa = 3.31 PSKK219 pKa = 11.25 NDD221 pKa = 2.94 NTRR224 pKa = 11.84 YY225 pKa = 10.0 FGTLTGGAQTPPVLSVTNTTTTILLDD251 pKa = 3.95 EE252 pKa = 4.77 YY253 pKa = 11.48 GVGPLCKK260 pKa = 10.15 GGRR263 pKa = 11.84 LYY265 pKa = 10.93 LAAADD270 pKa = 3.2 ICGFSTEE277 pKa = 4.39 STGKK281 pKa = 8.46 MRR283 pKa = 11.84 YY284 pKa = 9.24 RR285 pKa = 11.84 GLPRR289 pKa = 11.84 FFKK292 pKa = 10.74 VKK294 pKa = 9.57 LRR296 pKa = 11.84 KK297 pKa = 9.31 RR298 pKa = 11.84 AVKK301 pKa = 10.12 NPYY304 pKa = 10.41 AIGSLLNSFFSSFLPQVKK322 pKa = 8.75 GQPMTGDD329 pKa = 3.0 KK330 pKa = 11.05 GQVEE334 pKa = 4.93 EE335 pKa = 4.2 VTIFQGTEE343 pKa = 3.89 PLPADD348 pKa = 4.02 PDD350 pKa = 4.46 LIRR353 pKa = 11.84 SVDD356 pKa = 3.36 QFGQFLTKK364 pKa = 10.38 EE365 pKa = 3.93 PAQANVVV372 pKa = 3.43
Molecular weight: 40.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.671
IPC2_protein 5.842
IPC_protein 5.842
Toseland 5.957
ProMoST 6.109
Dawson 6.008
Bjellqvist 6.046
Wikipedia 5.97
Rodwell 5.97
Grimsley 5.982
Solomon 6.008
Lehninger 5.995
Nozaki 6.224
DTASelect 6.402
Thurlkill 6.338
EMBOSS 6.3
Sillero 6.326
Patrickios 4.177
IPC_peptide 6.02
IPC2_peptide 6.249
IPC2.peptide.svr19 6.428
Protein with the highest isoelectric point:
>tr|T2FFC5|T2FFC5_9POLY Major capsid protein VP1 OS=African elephant polyomavirus 1 OX=1399914 GN=AelPyV_gp1 PE=3 SV=1
MM1 pKa = 7.76 ALQLWFPPVDD11 pKa = 3.49 YY12 pKa = 10.53 LFPGVTQFSRR22 pKa = 11.84 FLRR25 pKa = 11.84 YY26 pKa = 9.0 IDD28 pKa = 3.49 PTQWGPEE35 pKa = 4.1 LFHH38 pKa = 7.14 HH39 pKa = 6.48 LTQAFFSTFRR49 pKa = 11.84 DD50 pKa = 3.37 ATRR53 pKa = 11.84 RR54 pKa = 11.84 QLTDD58 pKa = 2.99 ASRR61 pKa = 11.84 NIAEE65 pKa = 4.33 RR66 pKa = 11.84 TAKK69 pKa = 9.83 TLVEE73 pKa = 4.21 TLVDD77 pKa = 4.46 LLTNATWAIAHH88 pKa = 6.75 LPSDD92 pKa = 4.02 VYY94 pKa = 11.21 TFLDD98 pKa = 3.26 NYY100 pKa = 10.78 YY101 pKa = 10.89 EE102 pKa = 4.22 EE103 pKa = 5.58 LPPLNPAQFSGIRR116 pKa = 11.84 KK117 pKa = 9.35 RR118 pKa = 11.84 LGKK121 pKa = 10.16 VNPYY125 pKa = 8.73 EE126 pKa = 4.12 QQDD129 pKa = 3.7 VFANTVSAEE138 pKa = 3.99 FVTKK142 pKa = 9.27 VTAPGGAMQKK152 pKa = 9.93 HH153 pKa = 5.51 APDD156 pKa = 3.27 WVLPLLLGLFDD167 pKa = 3.75 EE168 pKa = 6.29 DD169 pKa = 3.92 YY170 pKa = 11.08 RR171 pKa = 11.84 QVTPRR176 pKa = 11.84 DD177 pKa = 3.21 GPAAKK182 pKa = 9.86 RR183 pKa = 11.84 RR184 pKa = 11.84 RR185 pKa = 11.84 TTLRR189 pKa = 11.84 TIQKK193 pKa = 7.69 TPPKK197 pKa = 8.96 TGKK200 pKa = 10.13 RR201 pKa = 11.84 PPNTRR206 pKa = 11.84 ARR208 pKa = 11.84 RR209 pKa = 11.84 SASSRR214 pKa = 11.84 SKK216 pKa = 10.74 KK217 pKa = 9.85 RR218 pKa = 11.84 AA219 pKa = 3.11
Molecular weight: 25.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 9.545
IPC_protein 10.014
Toseland 10.321
ProMoST 9.999
Dawson 10.482
Bjellqvist 10.16
Wikipedia 10.672
Rodwell 10.789
Grimsley 10.555
Solomon 10.54
Lehninger 10.511
Nozaki 10.292
DTASelect 10.16
Thurlkill 10.35
EMBOSS 10.716
Sillero 10.394
Patrickios 10.452
IPC_peptide 10.54
IPC2_peptide 8.799
IPC2.peptide.svr19 8.771
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
1
5
1750
171
658
350.0
39.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.686 ± 1.484
2.4 ± 0.905
5.657 ± 0.703
5.2 ± 0.241
5.314 ± 0.433
5.943 ± 0.692
2.114 ± 0.387
3.143 ± 0.431
5.886 ± 0.801
10.514 ± 0.426
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.4 ± 0.746
4.286 ± 0.545
6.4 ± 0.945
4.171 ± 0.31
6.057 ± 0.762
6.114 ± 0.276
7.314 ± 0.7
5.771 ± 0.672
1.371 ± 0.278
3.257 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here