Strigomonas culicis
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9986 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S9TIR3|S9TIR3_9TRYP Uncharacterized protein OS=Strigomonas culicis OX=28005 GN=STCU_10281 PE=4 SV=1
MM1 pKa = 8.1 DD2 pKa = 5.55 APLFNINDD10 pKa = 4.58 PLPAPPTNQPPHH22 pKa = 6.07 PTSGGSGADD31 pKa = 3.21 VDD33 pKa = 5.05 PYY35 pKa = 11.33 DD36 pKa = 4.22 SSGVDD41 pKa = 2.94 TADD44 pKa = 4.83 RR45 pKa = 11.84 SVCCDD50 pKa = 3.37 SDD52 pKa = 4.05 SSSDD56 pKa = 3.24 GSHH59 pKa = 6.92 RR60 pKa = 11.84 FHH62 pKa = 6.87 QLHH65 pKa = 6.32 SPQNPTEE72 pKa = 4.25 RR73 pKa = 11.84 SSMMYY78 pKa = 10.22 SSASADD84 pKa = 3.61 SLHH87 pKa = 6.53 PSSWDD92 pKa = 3.51 EE93 pKa = 3.8 YY94 pKa = 10.7 STDD97 pKa = 3.46 SDD99 pKa = 3.9 GG100 pKa = 4.63
Molecular weight: 10.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.948
IPC_protein 3.935
Toseland 3.706
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.948
Rodwell 3.757
Grimsley 3.617
Solomon 3.948
Lehninger 3.91
Nozaki 4.088
DTASelect 4.406
Thurlkill 3.783
EMBOSS 3.961
Sillero 4.075
Patrickios 0.947
IPC_peptide 3.935
IPC2_peptide 4.037
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|S9TEA4|S9TEA4_9TRYP Uncharacterized protein OS=Strigomonas culicis OX=28005 GN=STCU_12149 PE=4 SV=1
MM1 pKa = 7.42 SARR4 pKa = 11.84 ARR6 pKa = 11.84 AAAASVRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 PTSPRR20 pKa = 11.84 PRR22 pKa = 11.84 RR23 pKa = 11.84 SCRR26 pKa = 11.84 ATRR29 pKa = 11.84 RR30 pKa = 11.84 APRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SPCRR39 pKa = 11.84 WPEE42 pKa = 3.58 MRR44 pKa = 11.84 PRR46 pKa = 11.84 QPTYY50 pKa = 10.73 CSGSAAAPPRR60 pKa = 11.84 WRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 PARR67 pKa = 11.84 RR68 pKa = 11.84 FPGRR72 pKa = 11.84 ARR74 pKa = 11.84 RR75 pKa = 11.84 SPAPWRR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 RR84 pKa = 11.84 VAVVRR89 pKa = 11.84 TASAPRR95 pKa = 11.84 SRR97 pKa = 11.84 RR98 pKa = 11.84 APRR101 pKa = 11.84 TAGRR105 pKa = 11.84 GCPRR109 pKa = 11.84 GRR111 pKa = 11.84 ARR113 pKa = 11.84 GPSASRR119 pKa = 11.84 ARR121 pKa = 11.84 RR122 pKa = 11.84 AAPPPRR128 pKa = 11.84 RR129 pKa = 11.84 AAAAGGPPTATRR141 pKa = 11.84 GAAAARR147 pKa = 11.84 RR148 pKa = 11.84 RR149 pKa = 11.84 ATARR153 pKa = 11.84 RR154 pKa = 11.84 TSARR158 pKa = 11.84 SRR160 pKa = 11.84 RR161 pKa = 11.84 RR162 pKa = 11.84 WSCCCSSGRR171 pKa = 11.84 RR172 pKa = 11.84 SRR174 pKa = 11.84 RR175 pKa = 11.84 PRR177 pKa = 11.84 PRR179 pKa = 11.84 SARR182 pKa = 11.84 TRR184 pKa = 11.84 RR185 pKa = 11.84 PTRR188 pKa = 11.84 AARR191 pKa = 11.84 TAGGRR196 pKa = 11.84 TTRR199 pKa = 11.84 RR200 pKa = 11.84 RR201 pKa = 11.84 APPPSIARR209 pKa = 11.84 GTAAIGARR217 pKa = 11.84 RR218 pKa = 11.84 TTAIGARR225 pKa = 11.84 RR226 pKa = 11.84 TAAIGARR233 pKa = 11.84 RR234 pKa = 11.84 TAAISTRR241 pKa = 11.84 RR242 pKa = 11.84 AAAIRR247 pKa = 11.84 ARR249 pKa = 11.84 RR250 pKa = 11.84 TAAISARR257 pKa = 11.84 RR258 pKa = 11.84 TAAISARR265 pKa = 11.84 RR266 pKa = 11.84 TTAPAPAARR275 pKa = 11.84 SRR277 pKa = 11.84 TAAARR282 pKa = 11.84 PPPPVRR288 pKa = 11.84 RR289 pKa = 11.84 APAAPRR295 pKa = 11.84 GSAPRR300 pKa = 11.84 RR301 pKa = 11.84 RR302 pKa = 11.84 RR303 pKa = 11.84 RR304 pKa = 11.84 TASASGRR311 pKa = 11.84 RR312 pKa = 11.84 RR313 pKa = 11.84 RR314 pKa = 11.84 RR315 pKa = 11.84 RR316 pKa = 11.84 PRR318 pKa = 11.84 RR319 pKa = 11.84 PPKK322 pKa = 9.84 RR323 pKa = 11.84 SPRR326 pKa = 11.84 RR327 pKa = 11.84 AAAPPRR333 pKa = 11.84 ARR335 pKa = 11.84 APGRR339 pKa = 11.84 PPPRR343 pKa = 11.84 RR344 pKa = 11.84 TSPLRR349 pKa = 11.84 CSAASARR356 pKa = 11.84 SWTSS360 pKa = 2.74
Molecular weight: 39.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.228
IPC_protein 12.808
Toseland 12.969
ProMoST 13.466
Dawson 12.969
Bjellqvist 12.969
Wikipedia 13.451
Rodwell 12.471
Grimsley 13.013
Solomon 13.466
Lehninger 13.378
Nozaki 12.969
DTASelect 12.969
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.179
IPC_peptide 13.481
IPC2_peptide 12.471
IPC2.peptide.svr19 9.212
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9986
0
9986
3821174
47
4543
382.7
42.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.881 ± 0.041
1.868 ± 0.014
5.221 ± 0.018
6.406 ± 0.025
3.878 ± 0.017
6.286 ± 0.024
2.709 ± 0.013
3.972 ± 0.019
4.6 ± 0.021
9.673 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.43 ± 0.01
3.297 ± 0.017
5.11 ± 0.024
3.954 ± 0.017
6.989 ± 0.032
6.822 ± 0.03
5.589 ± 0.018
7.287 ± 0.02
1.061 ± 0.008
2.915 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here