Burkholderia sp. A9

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; unclassified Burkholderia

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3010 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B1YRV3|A0A0B1YRV3_9BURK Lysine transporter LysE OS=Burkholderia sp. A9 OX=1365108 GN=PI86_07385 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 5.15FKK4 pKa = 10.6LWQCVLCGFVYY15 pKa = 10.69DD16 pKa = 4.44EE17 pKa = 4.96AEE19 pKa = 4.08GMPAEE24 pKa = 5.47GIPAGTRR31 pKa = 11.84WADD34 pKa = 3.62VPDD37 pKa = 4.17DD38 pKa = 4.93WICPEE43 pKa = 4.34CSATKK48 pKa = 9.78IDD50 pKa = 4.29FEE52 pKa = 4.56MIEE55 pKa = 3.92II56 pKa = 4.27

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B1YUC9|A0A0B1YUC9_9BURK NAD-dependent dehydratase OS=Burkholderia sp. A9 OX=1365108 GN=PI86_02470 PE=3 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84APVGRR7 pKa = 11.84RR8 pKa = 11.84HH9 pKa = 5.6GRR11 pKa = 11.84TGRR14 pKa = 11.84RR15 pKa = 11.84MAHH18 pKa = 6.22RR19 pKa = 11.84PSGAGSPALSRR30 pKa = 11.84PASAKK35 pKa = 9.36RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84VRR40 pKa = 11.84HH41 pKa = 5.08WPAPACLARR50 pKa = 11.84RR51 pKa = 11.84SPAPHH56 pKa = 6.17APFRR60 pKa = 11.84IRR62 pKa = 11.84TRR64 pKa = 11.84RR65 pKa = 11.84LTRR68 pKa = 3.48

Molecular weight: 7.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3010

0

3010

940337

30

2517

312.4

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.234 ± 0.075

0.914 ± 0.013

5.72 ± 0.037

4.843 ± 0.04

3.678 ± 0.03

8.314 ± 0.048

2.302 ± 0.022

4.587 ± 0.031

3.009 ± 0.04

10.146 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.02

2.67 ± 0.031

5.121 ± 0.03

3.511 ± 0.027

7.111 ± 0.043

5.313 ± 0.031

5.465 ± 0.041

7.916 ± 0.035

1.36 ± 0.017

2.44 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski