Streptococcus virus 9871
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191KBM2|A0A191KBM2_9CAUD Uncharacterized protein OS=Streptococcus virus 9871 OX=1814957 GN=P9871_26 PE=4 SV=1
MM1 pKa = 7.12 VNWVDD6 pKa = 4.06 VNGNDD11 pKa = 4.75 LPDD14 pKa = 4.61 GADD17 pKa = 3.12 QDD19 pKa = 4.52 FKK21 pKa = 11.66 SGMFFSFASDD31 pKa = 3.27 EE32 pKa = 4.33 VNITDD37 pKa = 4.0 TGDD40 pKa = 2.69 GGYY43 pKa = 10.72 YY44 pKa = 9.75 GGYY47 pKa = 7.97 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.06 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.817
IPC2_protein 3.719
IPC_protein 3.668
Toseland 3.439
ProMoST 3.884
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.706
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.617
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.795
Patrickios 0.223
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|A0A191KBE0|A0A191KBE0_9CAUD Uncharacterized protein OS=Streptococcus virus 9871 OX=1814957 GN=P9871_49 PE=4 SV=1
MM1 pKa = 7.03 QVKK4 pKa = 9.86 EE5 pKa = 3.7 YY6 pKa = 10.91 ALYY9 pKa = 10.45 KK10 pKa = 10.33 GEE12 pKa = 4.7 EE13 pKa = 4.28 IIAMGTKK20 pKa = 10.0 SEE22 pKa = 4.06 IAKK25 pKa = 10.1 QLGISVRR32 pKa = 11.84 SVTCYY37 pKa = 7.69 GTPSYY42 pKa = 11.34 AKK44 pKa = 9.13 RR45 pKa = 11.84 TSEE48 pKa = 3.67 KK49 pKa = 10.45 DD50 pKa = 2.87 GRR52 pKa = 11.84 RR53 pKa = 11.84 LVKK56 pKa = 10.63 LL57 pKa = 3.55
Molecular weight: 6.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.121
IPC2_protein 9.063
IPC_protein 9.048
Toseland 9.663
ProMoST 9.399
Dawson 9.911
Bjellqvist 9.604
Wikipedia 10.087
Rodwell 10.262
Grimsley 9.984
Solomon 9.97
Lehninger 9.94
Nozaki 9.692
DTASelect 9.589
Thurlkill 9.75
EMBOSS 10.072
Sillero 9.838
Patrickios 7.585
IPC_peptide 9.97
IPC2_peptide 8.244
IPC2.peptide.svr19 8.062
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
10013
40
916
204.3
23.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.222 ± 0.381
0.749 ± 0.115
6.212 ± 0.238
7.241 ± 0.633
4.654 ± 0.242
6.122 ± 0.518
1.129 ± 0.128
6.801 ± 0.415
9.098 ± 0.521
7.79 ± 0.411
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.507 ± 0.179
6.412 ± 0.43
2.607 ± 0.209
4.055 ± 0.261
3.995 ± 0.341
6.502 ± 0.364
6.382 ± 0.27
6.042 ± 0.352
1.468 ± 0.202
4.015 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here