Amino acid dipepetide frequency for bacterium HR24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.736AlaAla: 15.736 ± 0.275
1.436AlaCys: 1.436 ± 0.059
5.077AlaAsp: 5.077 ± 0.097
7.361AlaGlu: 7.361 ± 0.131
3.697AlaPhe: 3.697 ± 0.088
10.332AlaGly: 10.332 ± 0.161
2.286AlaHis: 2.286 ± 0.06
4.938AlaIle: 4.938 ± 0.1
2.033AlaLys: 2.033 ± 0.06
15.631AlaLeu: 15.631 ± 0.227
2.743AlaMet: 2.743 ± 0.067
1.707AlaAsn: 1.707 ± 0.053
5.725AlaPro: 5.725 ± 0.106
4.279AlaGln: 4.279 ± 0.092
11.884AlaArg: 11.884 ± 0.168
5.459AlaSer: 5.459 ± 0.106
4.654AlaThr: 4.654 ± 0.089
10.476AlaVal: 10.476 ± 0.151
1.724AlaTrp: 1.724 ± 0.067
2.775AlaTyr: 2.775 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
0.901CysAla: 0.901 ± 0.041
0.124CysCys: 0.124 ± 0.014
0.484CysAsp: 0.484 ± 0.026
0.45CysGlu: 0.45 ± 0.029
0.34CysPhe: 0.34 ± 0.023
1.096CysGly: 1.096 ± 0.051
0.273CysHis: 0.273 ± 0.019
0.373CysIle: 0.373 ± 0.026
0.157CysLys: 0.157 ± 0.016
1.155CysLeu: 1.155 ± 0.046
0.15CysMet: 0.15 ± 0.015
0.251CysAsn: 0.251 ± 0.022
0.841CysPro: 0.841 ± 0.038
0.392CysGln: 0.392 ± 0.027
1.066CysArg: 1.066 ± 0.042
0.486CysSer: 0.486 ± 0.03
0.451CysThr: 0.451 ± 0.027
0.569CysVal: 0.569 ± 0.029
0.106CysTrp: 0.106 ± 0.012
0.311CysTyr: 0.311 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
5.088AspAla: 5.088 ± 0.094
0.481AspCys: 0.481 ± 0.029
2.38AspAsp: 2.38 ± 0.069
3.556AspGlu: 3.556 ± 0.085
1.545AspPhe: 1.545 ± 0.055
4.593AspGly: 4.593 ± 0.101
0.896AspHis: 0.896 ± 0.036
2.325AspIle: 2.325 ± 0.07
0.851AspLys: 0.851 ± 0.038
5.667AspLeu: 5.667 ± 0.09
1.079AspMet: 1.079 ± 0.045
0.81AspAsn: 0.81 ± 0.039
3.606AspPro: 3.606 ± 0.086
1.296AspGln: 1.296 ± 0.049
3.823AspArg: 3.823 ± 0.085
1.785AspSer: 1.785 ± 0.054
1.861AspThr: 1.861 ± 0.061
4.287AspVal: 4.287 ± 0.089
0.775AspTrp: 0.775 ± 0.035
1.524AspTyr: 1.524 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
9.58GluAla: 9.58 ± 0.155
0.459GluCys: 0.459 ± 0.027
2.996GluAsp: 2.996 ± 0.081
6.572GluGlu: 6.572 ± 0.161
1.654GluPhe: 1.654 ± 0.05
6.29GluGly: 6.29 ± 0.117
1.38GluHis: 1.38 ± 0.04
2.071GluIle: 2.071 ± 0.067
1.578GluLys: 1.578 ± 0.055
7.495GluLeu: 7.495 ± 0.156
1.869GluMet: 1.869 ± 0.063
0.965GluAsn: 0.965 ± 0.041
3.107GluPro: 3.107 ± 0.071
2.226GluGln: 2.226 ± 0.065
7.065GluArg: 7.065 ± 0.141
2.246GluSer: 2.246 ± 0.061
2.595GluThr: 2.595 ± 0.07
6.435GluVal: 6.435 ± 0.111
0.978GluTrp: 0.978 ± 0.041
1.349GluTyr: 1.349 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
3.165PheAla: 3.165 ± 0.074
0.364PheCys: 0.364 ± 0.024
1.593PheAsp: 1.593 ± 0.054
1.545PheGlu: 1.545 ± 0.047
1.163PhePhe: 1.163 ± 0.053
2.77PheGly: 2.77 ± 0.078
0.656PheHis: 0.656 ± 0.031
1.087PheIle: 1.087 ± 0.048
0.507PheLys: 0.507 ± 0.029
3.713PheLeu: 3.713 ± 0.102
0.598PheMet: 0.598 ± 0.032
0.74PheAsn: 0.74 ± 0.039
1.757PhePro: 1.757 ± 0.063
1.091PheGln: 1.091 ± 0.045
2.656PheArg: 2.656 ± 0.063
1.451PheSer: 1.451 ± 0.048
1.352PheThr: 1.352 ± 0.048
2.71PheVal: 2.71 ± 0.068
0.554PheTrp: 0.554 ± 0.035
1.018PheTyr: 1.018 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
8.97GlyAla: 8.97 ± 0.144
0.894GlyCys: 0.894 ± 0.036
4.179GlyAsp: 4.179 ± 0.101
5.624GlyGlu: 5.624 ± 0.095
2.608GlyPhe: 2.608 ± 0.071
7.99GlyGly: 7.99 ± 0.178
2.241GlyHis: 2.241 ± 0.067
4.094GlyIle: 4.094 ± 0.091
2.086GlyLys: 2.086 ± 0.065
10.35GlyLeu: 10.35 ± 0.158
2.195GlyMet: 2.195 ± 0.059
1.443GlyAsn: 1.443 ± 0.056
5.371GlyPro: 5.371 ± 0.101
3.662GlyGln: 3.662 ± 0.083
8.878GlyArg: 8.878 ± 0.132
4.132GlySer: 4.132 ± 0.098
4.236GlyThr: 4.236 ± 0.091
7.042GlyVal: 7.042 ± 0.107
1.425GlyTrp: 1.425 ± 0.044
2.512GlyTyr: 2.512 ± 0.066
0.0GlyXaa: 0.0 ± 0.0
His
1.78HisAla: 1.78 ± 0.062
0.238HisCys: 0.238 ± 0.019
1.074HisAsp: 1.074 ± 0.042
1.175HisGlu: 1.175 ± 0.048
0.749HisPhe: 0.749 ± 0.042
1.874HisGly: 1.874 ± 0.062
0.649HisHis: 0.649 ± 0.041
0.934HisIle: 0.934 ± 0.038
0.359HisLys: 0.359 ± 0.025
2.669HisLeu: 2.669 ± 0.071
0.388HisMet: 0.388 ± 0.024
0.412HisAsn: 0.412 ± 0.024
1.709HisPro: 1.709 ± 0.057
0.656HisGln: 0.656 ± 0.038
1.952HisArg: 1.952 ± 0.062
0.8HisSer: 0.8 ± 0.041
0.833HisThr: 0.833 ± 0.037
1.702HisVal: 1.702 ± 0.055
0.369HisTrp: 0.369 ± 0.027
0.651HisTyr: 0.651 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
4.855IleAla: 4.855 ± 0.083
0.408IleCys: 0.408 ± 0.027
2.21IleAsp: 2.21 ± 0.062
2.583IleGlu: 2.583 ± 0.075
1.22IlePhe: 1.22 ± 0.051
3.414IleGly: 3.414 ± 0.083
0.625IleHis: 0.625 ± 0.036
1.625IleIle: 1.625 ± 0.062
0.773IleLys: 0.773 ± 0.034
2.839IleLeu: 2.839 ± 0.073
0.778IleMet: 0.778 ± 0.038
0.935IleAsn: 0.935 ± 0.042
1.939IlePro: 1.939 ± 0.059
0.891IleGln: 0.891 ± 0.043
2.669IleArg: 2.669 ± 0.076
1.808IleSer: 1.808 ± 0.063
1.856IleThr: 1.856 ± 0.069
3.857IleVal: 3.857 ± 0.085
0.524IleTrp: 0.524 ± 0.033
0.992IleTyr: 0.992 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
2.438LysAla: 2.438 ± 0.066
0.129LysCys: 0.129 ± 0.015
0.896LysAsp: 0.896 ± 0.045
1.595LysGlu: 1.595 ± 0.06
0.459LysPhe: 0.459 ± 0.032
1.977LysGly: 1.977 ± 0.055
0.342LysHis: 0.342 ± 0.024
0.635LysIle: 0.635 ± 0.032
0.524LysLys: 0.524 ± 0.034
1.937LysLeu: 1.937 ± 0.058
0.395LysMet: 0.395 ± 0.024
0.364LysAsn: 0.364 ± 0.027
0.906LysPro: 0.906 ± 0.04
0.542LysGln: 0.542 ± 0.029
1.628LysArg: 1.628 ± 0.059
0.745LysSer: 0.745 ± 0.036
0.93LysThr: 0.93 ± 0.042
2.013LysVal: 2.013 ± 0.062
0.269LysTrp: 0.269 ± 0.023
0.478LysTyr: 0.478 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
16.644LeuAla: 16.644 ± 0.249
1.097LeuCys: 1.097 ± 0.039
5.634LeuAsp: 5.634 ± 0.108
8.162LeuGlu: 8.162 ± 0.166
3.004LeuPhe: 3.004 ± 0.081
10.659LeuGly: 10.659 ± 0.173
2.129LeuHis: 2.129 ± 0.07
2.93LeuIle: 2.93 ± 0.065
2.248LeuLys: 2.248 ± 0.075
12.345LeuLeu: 12.345 ± 0.24
2.282LeuMet: 2.282 ± 0.068
1.615LeuAsn: 1.615 ± 0.057
7.204LeuPro: 7.204 ± 0.119
3.472LeuGln: 3.472 ± 0.082
10.264LeuArg: 10.264 ± 0.147
5.644LeuSer: 5.644 ± 0.114
4.429LeuThr: 4.429 ± 0.087
10.093LeuVal: 10.093 ± 0.151
1.62LeuTrp: 1.62 ± 0.058
2.396LeuTyr: 2.396 ± 0.063
0.0LeuXaa: 0.0 ± 0.0
Met
3.439MetAla: 3.439 ± 0.082
0.142MetCys: 0.142 ± 0.015
1.112MetAsp: 1.112 ± 0.039
1.439MetGlu: 1.439 ± 0.053
0.516MetPhe: 0.516 ± 0.031
2.082MetGly: 2.082 ± 0.059
0.327MetHis: 0.327 ± 0.023
0.497MetIle: 0.497 ± 0.026
0.479MetLys: 0.479 ± 0.029
1.955MetLeu: 1.955 ± 0.066
0.398MetMet: 0.398 ± 0.025
0.392MetAsn: 0.392 ± 0.025
1.436MetPro: 1.436 ± 0.053
0.569MetGln: 0.569 ± 0.028
1.897MetArg: 1.897 ± 0.06
1.081MetSer: 1.081 ± 0.043
1.091MetThr: 1.091 ± 0.04
1.917MetVal: 1.917 ± 0.056
0.288MetTrp: 0.288 ± 0.021
0.377MetTyr: 0.377 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
1.899AsnAla: 1.899 ± 0.053
0.21AsnCys: 0.21 ± 0.019
0.793AsnAsp: 0.793 ± 0.038
0.8AsnGlu: 0.8 ± 0.033
0.633AsnPhe: 0.633 ± 0.032
1.398AsnGly: 1.398 ± 0.065
0.286AsnHis: 0.286 ± 0.021
0.836AsnIle: 0.836 ± 0.042
0.369AsnLys: 0.369 ± 0.027
1.939AsnLeu: 1.939 ± 0.064
0.362AsnMet: 0.362 ± 0.023
0.383AsnAsn: 0.383 ± 0.027
1.392AsnPro: 1.392 ± 0.055
0.459AsnGln: 0.459 ± 0.028
1.273AsnArg: 1.273 ± 0.044
0.683AsnSer: 0.683 ± 0.037
0.805AsnThr: 0.805 ± 0.044
1.689AsnVal: 1.689 ± 0.043
0.269AsnTrp: 0.269 ± 0.022
0.535AsnTyr: 0.535 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
6.521ProAla: 6.521 ± 0.11
0.544ProCys: 0.544 ± 0.028
3.679ProAsp: 3.679 ± 0.079
4.875ProGlu: 4.875 ± 0.098
2.033ProPhe: 2.033 ± 0.067
6.181ProGly: 6.181 ± 0.115
1.405ProHis: 1.405 ± 0.053
1.924ProIle: 1.924 ± 0.059
1.054ProLys: 1.054 ± 0.047
6.343ProLeu: 6.343 ± 0.105
1.193ProMet: 1.193 ± 0.046
1.109ProAsn: 1.109 ± 0.045
4.513ProPro: 4.513 ± 0.111
2.271ProGln: 2.271 ± 0.067
4.885ProArg: 4.885 ± 0.103
3.004ProSer: 3.004 ± 0.07
2.682ProThr: 2.682 ± 0.085
5.067ProVal: 5.067 ± 0.098
1.071ProTrp: 1.071 ± 0.043
1.676ProTyr: 1.676 ± 0.06
0.0ProXaa: 0.0 ± 0.0
Gln
4.637GlnAla: 4.637 ± 0.102
0.223GlnCys: 0.223 ± 0.019
1.534GlnAsp: 1.534 ± 0.055
3.094GlnGlu: 3.094 ± 0.078
0.846GlnPhe: 0.846 ± 0.039
3.285GlnGly: 3.285 ± 0.067
0.709GlnHis: 0.709 ± 0.034
0.977GlnIle: 0.977 ± 0.037
0.798GlnLys: 0.798 ± 0.035
2.783GlnLeu: 2.783 ± 0.071
0.79GlnMet: 0.79 ± 0.039
0.559GlnAsn: 0.559 ± 0.03
1.843GlnPro: 1.843 ± 0.058
1.363GlnGln: 1.363 ± 0.052
3.598GlnArg: 3.598 ± 0.089
1.188GlnSer: 1.188 ± 0.051
1.144GlnThr: 1.144 ± 0.045
3.94GlnVal: 3.94 ± 0.09
0.473GlnTrp: 0.473 ± 0.026
0.635GlnTyr: 0.635 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
9.701ArgAla: 9.701 ± 0.159
0.932ArgCys: 0.932 ± 0.046
4.092ArgAsp: 4.092 ± 0.091
7.027ArgGlu: 7.027 ± 0.139
2.905ArgPhe: 2.905 ± 0.072
6.959ArgGly: 6.959 ± 0.11
2.2ArgHis: 2.2 ± 0.07
3.418ArgIle: 3.418 ± 0.082
1.613ArgLys: 1.613 ± 0.057
11.742ArgLeu: 11.742 ± 0.169
1.841ArgMet: 1.841 ± 0.059
1.42ArgAsn: 1.42 ± 0.054
6.227ArgPro: 6.227 ± 0.127
4.14ArgGln: 4.14 ± 0.087
10.093ArgArg: 10.093 ± 0.174
3.599ArgSer: 3.599 ± 0.075
3.309ArgThr: 3.309 ± 0.078
6.72ArgVal: 6.72 ± 0.097
1.679ArgTrp: 1.679 ± 0.047
2.578ArgTyr: 2.578 ± 0.071
0.0ArgXaa: 0.0 ± 0.0
Ser
4.341SerAla: 4.341 ± 0.091
0.479SerCys: 0.479 ± 0.033
2.039SerAsp: 2.039 ± 0.065
2.35SerGlu: 2.35 ± 0.06
1.681SerPhe: 1.681 ± 0.056
4.217SerGly: 4.217 ± 0.084
0.907SerHis: 0.907 ± 0.035
1.625SerIle: 1.625 ± 0.053
0.711SerLys: 0.711 ± 0.035
5.835SerLeu: 5.835 ± 0.103
0.978SerMet: 0.978 ± 0.035
0.673SerAsn: 0.673 ± 0.031
3.563SerPro: 3.563 ± 0.076
1.489SerGln: 1.489 ± 0.05
3.821SerArg: 3.821 ± 0.083
2.058SerSer: 2.058 ± 0.077
1.77SerThr: 1.77 ± 0.062
3.216SerVal: 3.216 ± 0.09
0.749SerTrp: 0.749 ± 0.037
1.312SerTyr: 1.312 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
4.946ThrAla: 4.946 ± 0.096
0.451ThrCys: 0.451 ± 0.029
1.924ThrAsp: 1.924 ± 0.057
2.046ThrGlu: 2.046 ± 0.064
1.563ThrPhe: 1.563 ± 0.051
4.434ThrGly: 4.434 ± 0.085
0.768ThrHis: 0.768 ± 0.036
1.932ThrIle: 1.932 ± 0.069
0.744ThrLys: 0.744 ± 0.039
4.35ThrLeu: 4.35 ± 0.076
0.921ThrMet: 0.921 ± 0.042
0.778ThrAsn: 0.778 ± 0.037
3.122ThrPro: 3.122 ± 0.1
1.005ThrGln: 1.005 ± 0.041
2.76ThrArg: 2.76 ± 0.066
1.869ThrSer: 1.869 ± 0.061
2.024ThrThr: 2.024 ± 0.066
4.426ThrVal: 4.426 ± 0.091
0.664ThrTrp: 0.664 ± 0.039
1.266ThrTyr: 1.266 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
10.896ValAla: 10.896 ± 0.173
0.863ValCys: 0.863 ± 0.039
4.378ValAsp: 4.378 ± 0.095
6.065ValGlu: 6.065 ± 0.113
2.532ValPhe: 2.532 ± 0.065
6.827ValGly: 6.827 ± 0.108
1.829ValHis: 1.829 ± 0.058
3.29ValIle: 3.29 ± 0.08
1.656ValLys: 1.656 ± 0.06
10.238ValLeu: 10.238 ± 0.179
1.735ValMet: 1.735 ± 0.057
1.601ValAsn: 1.601 ± 0.053
5.391ValPro: 5.391 ± 0.092
2.902ValGln: 2.902 ± 0.078
7.795ValArg: 7.795 ± 0.122
3.922ValSer: 3.922 ± 0.082
4.217ValThr: 4.217 ± 0.105
8.518ValVal: 8.518 ± 0.139
1.139ValTrp: 1.139 ± 0.04
2.011ValTyr: 2.011 ± 0.068
0.0ValXaa: 0.0 ± 0.0
Trp
1.886TrpAla: 1.886 ± 0.068
0.162TrpCys: 0.162 ± 0.018
0.767TrpAsp: 0.767 ± 0.039
1.069TrpGlu: 1.069 ± 0.047
0.446TrpPhe: 0.446 ± 0.031
1.277TrpGly: 1.277 ± 0.052
0.378TrpHis: 0.378 ± 0.027
0.312TrpIle: 0.312 ± 0.025
0.245TrpLys: 0.245 ± 0.02
1.925TrpLeu: 1.925 ± 0.068
0.25TrpMet: 0.25 ± 0.021
0.271TrpAsn: 0.271 ± 0.023
0.924TrpPro: 0.924 ± 0.04
0.901TrpGln: 0.901 ± 0.043
1.552TrpArg: 1.552 ± 0.052
0.681TrpSer: 0.681 ± 0.041
0.702TrpThr: 0.702 ± 0.036
1.025TrpVal: 1.025 ± 0.039
0.321TrpTrp: 0.321 ± 0.027
0.317TrpTyr: 0.317 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.527TyrAla: 2.527 ± 0.058
0.339TyrCys: 0.339 ± 0.026
1.387TyrAsp: 1.387 ± 0.048
1.522TyrGlu: 1.522 ± 0.052
0.896TyrPhe: 0.896 ± 0.044
2.274TyrGly: 2.274 ± 0.061
0.686TyrHis: 0.686 ± 0.034
0.978TyrIle: 0.978 ± 0.037
0.416TyrLys: 0.416 ± 0.03
2.96TyrLeu: 2.96 ± 0.071
0.486TyrMet: 0.486 ± 0.028
0.585TyrAsn: 0.585 ± 0.031
1.456TyrPro: 1.456 ± 0.049
0.813TyrGln: 0.813 ± 0.037
2.638TyrArg: 2.638 ± 0.077
1.175TyrSer: 1.175 ± 0.047
1.053TyrThr: 1.053 ± 0.042
2.105TyrVal: 2.105 ± 0.057
0.416TyrTrp: 0.416 ± 0.026
0.848TyrTyr: 0.848 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2129 proteins (605099 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski