Breda virus 1 (BRV-1)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Tornidovirineae; Tobaniviridae; Torovirinae; Torovirus; Renitovirus; Bovine torovirus; Breda virus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P0C6F4|R1A_BRV1 Replicase polyprotein 1a OS=Breda virus 1 OX=360393 GN=1a PE=3 SV=1
MM1 pKa = 7.64LSLILFFPSFAFAATPVTPYY21 pKa = 10.05YY22 pKa = 10.85GPGHH26 pKa = 6.45ITFDD30 pKa = 2.96WCGFGDD36 pKa = 4.6SRR38 pKa = 11.84SDD40 pKa = 3.4CTNPQSPMSLDD51 pKa = 3.52IPQQLCPKK59 pKa = 9.3FSSKK63 pKa = 10.56SSSSMFLSLHH73 pKa = 5.92WNNHH77 pKa = 4.49SSFVSYY83 pKa = 10.79DD84 pKa = 3.41YY85 pKa = 10.99FNCGVEE91 pKa = 4.1KK92 pKa = 10.87VFYY95 pKa = 10.1EE96 pKa = 4.4GVNFSPRR103 pKa = 11.84KK104 pKa = 9.63QYY106 pKa = 10.99SCWDD110 pKa = 3.52EE111 pKa = 5.57GVDD114 pKa = 3.97GWIEE118 pKa = 4.07LKK120 pKa = 10.03TRR122 pKa = 11.84FYY124 pKa = 10.93TKK126 pKa = 9.92LYY128 pKa = 10.95QMATTSRR135 pKa = 11.84CIKK138 pKa = 10.23LIQLQAPSSLPTLQAGVCRR157 pKa = 11.84TNKK160 pKa = 9.59QLPDD164 pKa = 3.62NPRR167 pKa = 11.84LALLSDD173 pKa = 4.13TVPTSVQFVLPGSSGTTICTKK194 pKa = 10.43HH195 pKa = 6.21LVPFCYY201 pKa = 10.16LNHH204 pKa = 6.67GCFTTGGSCLPFGVSYY220 pKa = 11.25VSDD223 pKa = 3.46SFYY226 pKa = 11.05YY227 pKa = 10.33GYY229 pKa = 11.38YY230 pKa = 10.49DD231 pKa = 3.59ATPQIGSTEE240 pKa = 3.98SHH242 pKa = 7.45DD243 pKa = 4.07YY244 pKa = 10.11VCDD247 pKa = 3.7YY248 pKa = 11.57LFMEE252 pKa = 5.47PGTYY256 pKa = 9.82NASTVGKK263 pKa = 9.6FLVYY267 pKa = 8.18PTKK270 pKa = 10.33SYY272 pKa = 11.54CMDD275 pKa = 3.38TMNITVPVQAVQSIWSEE292 pKa = 4.19QYY294 pKa = 11.3ASDD297 pKa = 4.15DD298 pKa = 5.34AIGQACKK305 pKa = 10.37APYY308 pKa = 10.08CIFYY312 pKa = 11.06NKK314 pKa = 6.93TTPYY318 pKa = 9.94TVTNGSDD325 pKa = 3.28ANHH328 pKa = 7.09GDD330 pKa = 3.56DD331 pKa = 4.3EE332 pKa = 4.86VRR334 pKa = 11.84MMMQGLLRR342 pKa = 11.84NSSCISPQGSTPLALYY358 pKa = 7.87STEE361 pKa = 4.51MIYY364 pKa = 10.68EE365 pKa = 4.21PNYY368 pKa = 9.67GSCPQFYY375 pKa = 10.87KK376 pKa = 11.08LFDD379 pKa = 3.36TSGNEE384 pKa = 4.06NIDD387 pKa = 4.31VISSSYY393 pKa = 10.38FVATWVLLVVVVILIFVIISFFCC416 pKa = 4.13

Molecular weight: 46.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0C0V9|HEMA_BRV1 Hemagglutinin-esterase OS=Breda virus 1 OX=360393 GN=HE PE=1 SV=1
MM1 pKa = 7.76NSMLNPNAVPCQPSPQVVAIPMQYY25 pKa = 10.46PSGFSPGFRR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84NPGFRR42 pKa = 11.84PMFNRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84NNNGNQNRR57 pKa = 11.84GRR59 pKa = 11.84QNRR62 pKa = 11.84QRR64 pKa = 11.84VQNNNRR70 pKa = 11.84GNIRR74 pKa = 11.84NRR76 pKa = 11.84QNNGQRR82 pKa = 11.84GNRR85 pKa = 11.84RR86 pKa = 11.84QYY88 pKa = 10.08NQPSPNVPFEE98 pKa = 4.02QQLLMMANEE107 pKa = 3.96TAYY110 pKa = 10.53AATYY114 pKa = 8.79PPEE117 pKa = 4.19MQNVAPTKK125 pKa = 9.86LVKK128 pKa = 9.38IAKK131 pKa = 8.54RR132 pKa = 11.84AAMQIVSGHH141 pKa = 5.28ATVEE145 pKa = 4.05ISNGTEE151 pKa = 3.76DD152 pKa = 3.39SNKK155 pKa = 9.84RR156 pKa = 11.84VATFTIKK163 pKa = 10.74VVMNN167 pKa = 4.55

Molecular weight: 18.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

13577

167

6733

2262.8

256.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.81 ± 0.617

3.256 ± 0.239

5.266 ± 0.68

4.309 ± 0.459

6.194 ± 0.329

5.075 ± 0.133

2.21 ± 0.233

4.456 ± 0.463

4.92 ± 0.592

9.951 ± 0.491

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.203

4.382 ± 0.446

4.935 ± 0.339

4.611 ± 0.321

3.609 ± 0.266

7.785 ± 0.658

5.738 ± 0.603

9.789 ± 0.806

1.451 ± 0.171

5.112 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski