Vibrio phage VpKK5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088FAI7|A0A088FAI7_9CAUD Uncharacterized protein OS=Vibrio phage VpKK5 OX=1538804 PE=4 SV=1
MM1 pKa = 6.48TTPNEE6 pKa = 4.0RR7 pKa = 11.84HH8 pKa = 6.18SLVDD12 pKa = 4.54PEE14 pKa = 4.91DD15 pKa = 4.43CEE17 pKa = 5.15CEE19 pKa = 3.91EE20 pKa = 4.33TQYY23 pKa = 11.34HH24 pKa = 5.6YY25 pKa = 11.14APCQAQIEE33 pKa = 4.5VDD35 pKa = 3.33MKK37 pKa = 10.52TEE39 pKa = 3.94EE40 pKa = 4.01EE41 pKa = 4.44AMCNCCDD48 pKa = 3.71ACRR51 pKa = 11.84QACEE55 pKa = 3.98DD56 pKa = 4.67CII58 pKa = 4.28

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088F7Z6|A0A088F7Z6_9CAUD Tail assembly protein OS=Vibrio phage VpKK5 OX=1538804 PE=4 SV=1
MM1 pKa = 7.56VGVTMKK7 pKa = 10.46RR8 pKa = 11.84VRR10 pKa = 11.84ATKK13 pKa = 10.17RR14 pKa = 11.84GIQLWVDD21 pKa = 3.19AASYY25 pKa = 5.93TTRR28 pKa = 11.84AFYY31 pKa = 10.48IRR33 pKa = 11.84VKK35 pKa = 11.18DD36 pKa = 3.54NFIEE40 pKa = 4.43VGRR43 pKa = 11.84CDD45 pKa = 5.3RR46 pKa = 11.84NACYY50 pKa = 10.22NPKK53 pKa = 9.49TIKK56 pKa = 9.79TMQAKK61 pKa = 9.77ARR63 pKa = 11.84RR64 pKa = 11.84LLL66 pKa = 3.82

Molecular weight: 7.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

17036

45

1056

212.9

23.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.617 ± 0.416

1.274 ± 0.17

6.228 ± 0.192

6.727 ± 0.246

3.686 ± 0.161

7.707 ± 0.252

1.896 ± 0.146

5.459 ± 0.183

4.937 ± 0.304

7.919 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.953 ± 0.157

4.502 ± 0.266

4.59 ± 0.199

4.761 ± 0.316

5.436 ± 0.257

5.289 ± 0.198

6.116 ± 0.251

6.821 ± 0.23

1.667 ± 0.153

3.416 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski