Amino acid dipepetide frequency for Vibrio phage VpKK5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.631AlaAla: 7.631 ± 0.788
1.057AlaCys: 1.057 ± 0.262
5.283AlaAsp: 5.283 ± 0.626
5.342AlaGlu: 5.342 ± 0.544
2.994AlaPhe: 2.994 ± 0.403
7.807AlaGly: 7.807 ± 0.578
0.822AlaHis: 0.822 ± 0.185
5.694AlaIle: 5.694 ± 0.56
4.05AlaLys: 4.05 ± 0.587
7.397AlaLeu: 7.397 ± 0.806
3.17AlaMet: 3.17 ± 0.417
3.581AlaAsn: 3.581 ± 0.514
3.287AlaPro: 3.287 ± 0.426
3.17AlaGln: 3.17 ± 0.426
4.461AlaArg: 4.461 ± 0.478
5.048AlaSer: 5.048 ± 0.507
6.34AlaThr: 6.34 ± 0.62
4.814AlaVal: 4.814 ± 0.597
1.702AlaTrp: 1.702 ± 0.322
2.524AlaTyr: 2.524 ± 0.405
0.0AlaXaa: 0.0 ± 0.0
Cys
0.47CysAla: 0.47 ± 0.158
0.235CysCys: 0.235 ± 0.124
1.233CysAsp: 1.233 ± 0.253
1.174CysGlu: 1.174 ± 0.355
0.587CysPhe: 0.587 ± 0.189
0.646CysGly: 0.646 ± 0.241
0.763CysHis: 0.763 ± 0.247
0.235CysIle: 0.235 ± 0.117
0.998CysLys: 0.998 ± 0.245
0.822CysLeu: 0.822 ± 0.22
0.352CysMet: 0.352 ± 0.131
0.646CysAsn: 0.646 ± 0.187
0.587CysPro: 0.587 ± 0.176
0.646CysGln: 0.646 ± 0.215
0.881CysArg: 0.881 ± 0.258
0.587CysSer: 0.587 ± 0.165
0.704CysThr: 0.704 ± 0.202
0.646CysVal: 0.646 ± 0.184
0.059CysTrp: 0.059 ± 0.051
0.47CysTyr: 0.47 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
5.87AspAla: 5.87 ± 0.672
0.646AspCys: 0.646 ± 0.229
3.64AspAsp: 3.64 ± 0.703
3.816AspGlu: 3.816 ± 0.483
2.231AspPhe: 2.231 ± 0.37
5.518AspGly: 5.518 ± 0.563
1.233AspHis: 1.233 ± 0.319
3.111AspIle: 3.111 ± 0.457
2.055AspLys: 2.055 ± 0.348
6.281AspLeu: 6.281 ± 0.652
1.468AspMet: 1.468 ± 0.362
1.878AspAsn: 1.878 ± 0.364
3.874AspPro: 3.874 ± 0.386
3.111AspGln: 3.111 ± 0.432
2.876AspArg: 2.876 ± 0.382
3.816AspSer: 3.816 ± 0.396
3.287AspThr: 3.287 ± 0.616
3.933AspVal: 3.933 ± 0.46
1.233AspTrp: 1.233 ± 0.247
2.407AspTyr: 2.407 ± 0.341
0.0AspXaa: 0.0 ± 0.0
Glu
5.753GluAla: 5.753 ± 0.751
0.998GluCys: 0.998 ± 0.231
4.344GluAsp: 4.344 ± 0.519
5.048GluGlu: 5.048 ± 0.864
2.524GluPhe: 2.524 ± 0.432
4.931GluGly: 4.931 ± 0.542
1.233GluHis: 1.233 ± 0.255
4.52GluIle: 4.52 ± 0.513
2.818GluLys: 2.818 ± 0.409
7.573GluLeu: 7.573 ± 0.752
2.642GluMet: 2.642 ± 0.42
2.348GluAsn: 2.348 ± 0.411
1.82GluPro: 1.82 ± 0.302
2.876GluGln: 2.876 ± 0.486
2.935GluArg: 2.935 ± 0.488
2.876GluSer: 2.876 ± 0.378
2.994GluThr: 2.994 ± 0.361
5.518GluVal: 5.518 ± 0.534
1.115GluTrp: 1.115 ± 0.242
2.407GluTyr: 2.407 ± 0.333
0.0GluXaa: 0.0 ± 0.0
Phe
3.816PheAla: 3.816 ± 0.477
0.294PheCys: 0.294 ± 0.14
2.818PheAsp: 2.818 ± 0.401
2.113PheGlu: 2.113 ± 0.339
1.057PhePhe: 1.057 ± 0.242
3.053PheGly: 3.053 ± 0.372
0.704PheHis: 0.704 ± 0.231
2.524PheIle: 2.524 ± 0.367
2.289PheLys: 2.289 ± 0.34
2.113PheLeu: 2.113 ± 0.405
1.115PheMet: 1.115 ± 0.24
1.82PheAsn: 1.82 ± 0.329
1.644PhePro: 1.644 ± 0.304
1.409PheGln: 1.409 ± 0.269
2.172PheArg: 2.172 ± 0.465
1.996PheSer: 1.996 ± 0.344
2.289PheThr: 2.289 ± 0.406
2.7PheVal: 2.7 ± 0.402
0.352PheTrp: 0.352 ± 0.117
0.528PheTyr: 0.528 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
5.694GlyAla: 5.694 ± 0.621
1.291GlyCys: 1.291 ± 0.321
4.227GlyAsp: 4.227 ± 0.458
5.401GlyGlu: 5.401 ± 0.504
2.642GlyPhe: 2.642 ± 0.298
6.457GlyGly: 6.457 ± 0.975
1.878GlyHis: 1.878 ± 0.359
3.874GlyIle: 3.874 ± 0.335
4.285GlyLys: 4.285 ± 0.647
5.577GlyLeu: 5.577 ± 0.591
2.759GlyMet: 2.759 ± 0.404
2.876GlyAsn: 2.876 ± 0.418
1.937GlyPro: 1.937 ± 0.322
3.346GlyGln: 3.346 ± 0.428
4.403GlyArg: 4.403 ± 0.517
4.109GlySer: 4.109 ± 0.551
5.753GlyThr: 5.753 ± 0.596
5.459GlyVal: 5.459 ± 0.674
1.82GlyTrp: 1.82 ± 0.447
3.287GlyTyr: 3.287 ± 0.472
0.0GlyXaa: 0.0 ± 0.0
His
1.585HisAla: 1.585 ± 0.296
0.294HisCys: 0.294 ± 0.14
0.822HisAsp: 0.822 ± 0.204
1.174HisGlu: 1.174 ± 0.262
0.763HisPhe: 0.763 ± 0.224
1.526HisGly: 1.526 ± 0.279
0.411HisHis: 0.411 ± 0.165
1.057HisIle: 1.057 ± 0.24
1.233HisLys: 1.233 ± 0.239
1.761HisLeu: 1.761 ± 0.337
0.528HisMet: 0.528 ± 0.211
0.939HisAsn: 0.939 ± 0.229
1.174HisPro: 1.174 ± 0.271
0.939HisGln: 0.939 ± 0.264
0.881HisArg: 0.881 ± 0.216
1.115HisSer: 1.115 ± 0.254
0.704HisThr: 0.704 ± 0.193
0.822HisVal: 0.822 ± 0.229
0.528HisTrp: 0.528 ± 0.161
0.704HisTyr: 0.704 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
5.753IleAla: 5.753 ± 0.583
0.939IleCys: 0.939 ± 0.274
3.757IleAsp: 3.757 ± 0.513
3.64IleGlu: 3.64 ± 0.521
2.055IlePhe: 2.055 ± 0.365
4.285IleGly: 4.285 ± 0.449
0.881IleHis: 0.881 ± 0.182
1.937IleIle: 1.937 ± 0.305
2.876IleLys: 2.876 ± 0.429
3.757IleLeu: 3.757 ± 0.535
1.702IleMet: 1.702 ± 0.272
2.348IleAsn: 2.348 ± 0.429
3.17IlePro: 3.17 ± 0.396
2.7IleGln: 2.7 ± 0.353
2.994IleArg: 2.994 ± 0.348
2.348IleSer: 2.348 ± 0.355
3.64IleThr: 3.64 ± 0.406
3.17IleVal: 3.17 ± 0.38
0.763IleTrp: 0.763 ± 0.218
1.878IleTyr: 1.878 ± 0.331
0.0IleXaa: 0.0 ± 0.0
Lys
4.461LysAla: 4.461 ± 0.623
0.646LysCys: 0.646 ± 0.235
2.642LysAsp: 2.642 ± 0.512
3.17LysGlu: 3.17 ± 0.398
1.233LysPhe: 1.233 ± 0.279
3.463LysGly: 3.463 ± 0.454
0.939LysHis: 0.939 ± 0.189
2.935LysIle: 2.935 ± 0.453
2.7LysLys: 2.7 ± 0.612
3.346LysLeu: 3.346 ± 0.447
1.996LysMet: 1.996 ± 0.318
1.702LysAsn: 1.702 ± 0.302
3.111LysPro: 3.111 ± 0.437
3.17LysGln: 3.17 ± 0.434
2.466LysArg: 2.466 ± 0.455
2.231LysSer: 2.231 ± 0.346
3.64LysThr: 3.64 ± 0.542
3.287LysVal: 3.287 ± 0.465
0.881LysTrp: 0.881 ± 0.287
1.35LysTyr: 1.35 ± 0.295
0.0LysXaa: 0.0 ± 0.0
Leu
7.397LeuAla: 7.397 ± 0.673
0.822LeuCys: 0.822 ± 0.227
5.988LeuAsp: 5.988 ± 0.606
6.281LeuGlu: 6.281 ± 0.701
2.994LeuPhe: 2.994 ± 0.409
6.046LeuGly: 6.046 ± 0.54
1.291LeuHis: 1.291 ± 0.273
4.285LeuIle: 4.285 ± 0.674
3.17LeuLys: 3.17 ± 0.413
4.814LeuLeu: 4.814 ± 0.518
1.115LeuMet: 1.115 ± 0.246
4.05LeuAsn: 4.05 ± 0.4
4.05LeuPro: 4.05 ± 0.575
3.229LeuGln: 3.229 ± 0.419
5.225LeuArg: 5.225 ± 0.574
4.696LeuSer: 4.696 ± 0.424
4.638LeuThr: 4.638 ± 0.589
5.577LeuVal: 5.577 ± 0.508
1.115LeuTrp: 1.115 ± 0.2
2.407LeuTyr: 2.407 ± 0.401
0.0LeuXaa: 0.0 ± 0.0
Met
2.876MetAla: 2.876 ± 0.369
0.294MetCys: 0.294 ± 0.115
1.585MetAsp: 1.585 ± 0.484
1.761MetGlu: 1.761 ± 0.255
1.057MetPhe: 1.057 ± 0.249
1.761MetGly: 1.761 ± 0.303
0.939MetHis: 0.939 ± 0.224
1.115MetIle: 1.115 ± 0.256
1.82MetLys: 1.82 ± 0.343
1.878MetLeu: 1.878 ± 0.376
0.939MetMet: 0.939 ± 0.211
1.409MetAsn: 1.409 ± 0.312
1.174MetPro: 1.174 ± 0.265
1.585MetGln: 1.585 ± 0.335
1.644MetArg: 1.644 ± 0.287
1.937MetSer: 1.937 ± 0.349
2.231MetThr: 2.231 ± 0.383
2.407MetVal: 2.407 ± 0.366
0.117MetTrp: 0.117 ± 0.075
0.998MetTyr: 0.998 ± 0.253
0.0MetXaa: 0.0 ± 0.0
Asn
3.581AsnAla: 3.581 ± 0.533
0.704AsnCys: 0.704 ± 0.233
2.407AsnAsp: 2.407 ± 0.443
2.642AsnGlu: 2.642 ± 0.38
1.409AsnPhe: 1.409 ± 0.223
4.872AsnGly: 4.872 ± 0.553
0.822AsnHis: 0.822 ± 0.193
2.231AsnIle: 2.231 ± 0.328
1.761AsnLys: 1.761 ± 0.403
3.933AsnLeu: 3.933 ± 0.427
1.291AsnMet: 1.291 ± 0.235
1.585AsnAsn: 1.585 ± 0.356
2.759AsnPro: 2.759 ± 0.427
3.17AsnGln: 3.17 ± 0.843
1.937AsnArg: 1.937 ± 0.307
2.113AsnSer: 2.113 ± 0.409
2.583AsnThr: 2.583 ± 0.429
2.642AsnVal: 2.642 ± 0.339
0.998AsnTrp: 0.998 ± 0.243
1.585AsnTyr: 1.585 ± 0.289
0.0AsnXaa: 0.0 ± 0.0
Pro
4.579ProAla: 4.579 ± 0.588
0.646ProCys: 0.646 ± 0.163
2.642ProAsp: 2.642 ± 0.316
4.755ProGlu: 4.755 ± 0.397
2.407ProPhe: 2.407 ± 0.309
1.409ProGly: 1.409 ± 0.335
0.646ProHis: 0.646 ± 0.191
2.231ProIle: 2.231 ± 0.338
2.583ProLys: 2.583 ± 0.399
3.287ProLeu: 3.287 ± 0.552
1.115ProMet: 1.115 ± 0.24
2.172ProAsn: 2.172 ± 0.425
2.348ProPro: 2.348 ± 0.341
1.996ProGln: 1.996 ± 0.317
2.289ProArg: 2.289 ± 0.346
2.172ProSer: 2.172 ± 0.337
2.759ProThr: 2.759 ± 0.465
3.346ProVal: 3.346 ± 0.462
0.822ProTrp: 0.822 ± 0.166
1.702ProTyr: 1.702 ± 0.342
0.0ProXaa: 0.0 ± 0.0
Gln
3.757GlnAla: 3.757 ± 0.431
0.646GlnCys: 0.646 ± 0.212
2.348GlnAsp: 2.348 ± 0.314
2.994GlnGlu: 2.994 ± 0.438
1.761GlnPhe: 1.761 ± 0.319
4.05GlnGly: 4.05 ± 0.586
0.763GlnHis: 0.763 ± 0.189
2.818GlnIle: 2.818 ± 0.433
1.644GlnLys: 1.644 ± 0.305
3.933GlnLeu: 3.933 ± 0.442
1.526GlnMet: 1.526 ± 0.344
2.818GlnAsn: 2.818 ± 0.959
2.055GlnPro: 2.055 ± 0.346
3.405GlnGln: 3.405 ± 1.008
2.466GlnArg: 2.466 ± 0.392
2.524GlnSer: 2.524 ± 0.456
2.407GlnThr: 2.407 ± 0.392
3.053GlnVal: 3.053 ± 0.389
0.528GlnTrp: 0.528 ± 0.165
2.113GlnTyr: 2.113 ± 0.353
0.0GlnXaa: 0.0 ± 0.0
Arg
3.933ArgAla: 3.933 ± 0.494
0.294ArgCys: 0.294 ± 0.123
3.053ArgAsp: 3.053 ± 0.504
3.463ArgGlu: 3.463 ± 0.393
2.466ArgPhe: 2.466 ± 0.421
3.229ArgGly: 3.229 ± 0.434
1.057ArgHis: 1.057 ± 0.296
3.522ArgIle: 3.522 ± 0.427
3.581ArgLys: 3.581 ± 0.54
4.52ArgLeu: 4.52 ± 0.555
1.702ArgMet: 1.702 ± 0.288
2.642ArgAsn: 2.642 ± 0.356
2.348ArgPro: 2.348 ± 0.357
2.524ArgGln: 2.524 ± 0.41
3.64ArgArg: 3.64 ± 0.63
3.405ArgSer: 3.405 ± 0.466
3.463ArgThr: 3.463 ± 0.446
2.994ArgVal: 2.994 ± 0.419
0.704ArgTrp: 0.704 ± 0.221
1.82ArgTyr: 1.82 ± 0.302
0.0ArgXaa: 0.0 ± 0.0
Ser
4.755SerAla: 4.755 ± 0.533
0.176SerCys: 0.176 ± 0.1
3.346SerAsp: 3.346 ± 0.471
2.935SerGlu: 2.935 ± 0.475
2.055SerPhe: 2.055 ± 0.355
3.992SerGly: 3.992 ± 0.493
1.115SerHis: 1.115 ± 0.25
2.466SerIle: 2.466 ± 0.385
2.876SerLys: 2.876 ± 0.392
4.52SerLeu: 4.52 ± 0.378
1.409SerMet: 1.409 ± 0.274
2.876SerAsn: 2.876 ± 0.464
2.055SerPro: 2.055 ± 0.297
3.053SerGln: 3.053 ± 0.455
2.524SerArg: 2.524 ± 0.318
3.111SerSer: 3.111 ± 0.478
2.583SerThr: 2.583 ± 0.442
4.05SerVal: 4.05 ± 0.546
0.998SerTrp: 0.998 ± 0.241
1.996SerTyr: 1.996 ± 0.265
0.0SerXaa: 0.0 ± 0.0
Thr
4.696ThrAla: 4.696 ± 0.553
1.115ThrCys: 1.115 ± 0.296
3.64ThrAsp: 3.64 ± 0.53
3.463ThrGlu: 3.463 ± 0.489
2.113ThrPhe: 2.113 ± 0.336
5.518ThrGly: 5.518 ± 0.772
0.528ThrHis: 0.528 ± 0.22
2.935ThrIle: 2.935 ± 0.395
3.111ThrLys: 3.111 ± 0.487
4.931ThrLeu: 4.931 ± 0.533
1.82ThrMet: 1.82 ± 0.342
2.876ThrAsn: 2.876 ± 0.427
2.876ThrPro: 2.876 ± 0.494
2.113ThrGln: 2.113 ± 0.287
3.522ThrArg: 3.522 ± 0.549
3.698ThrSer: 3.698 ± 0.522
3.874ThrThr: 3.874 ± 0.594
5.048ThrVal: 5.048 ± 0.599
0.939ThrTrp: 0.939 ± 0.191
2.348ThrTyr: 2.348 ± 0.445
0.0ThrXaa: 0.0 ± 0.0
Val
6.164ValAla: 6.164 ± 0.744
0.587ValCys: 0.587 ± 0.179
5.048ValAsp: 5.048 ± 0.57
4.931ValGlu: 4.931 ± 0.497
2.583ValPhe: 2.583 ± 0.437
5.225ValGly: 5.225 ± 0.5
1.35ValHis: 1.35 ± 0.272
4.461ValIle: 4.461 ± 0.601
3.405ValLys: 3.405 ± 0.407
4.461ValLeu: 4.461 ± 0.424
1.761ValMet: 1.761 ± 0.409
3.17ValAsn: 3.17 ± 0.512
3.287ValPro: 3.287 ± 0.363
3.463ValGln: 3.463 ± 0.462
3.933ValArg: 3.933 ± 0.399
2.994ValSer: 2.994 ± 0.485
3.64ValThr: 3.64 ± 0.448
4.696ValVal: 4.696 ± 0.62
0.998ValTrp: 0.998 ± 0.22
2.055ValTyr: 2.055 ± 0.345
0.0ValXaa: 0.0 ± 0.0
Trp
0.998TrpAla: 0.998 ± 0.195
0.235TrpCys: 0.235 ± 0.118
1.115TrpAsp: 1.115 ± 0.292
1.35TrpGlu: 1.35 ± 0.313
0.763TrpPhe: 0.763 ± 0.176
0.881TrpGly: 0.881 ± 0.275
0.47TrpHis: 0.47 ± 0.188
0.646TrpIle: 0.646 ± 0.17
0.411TrpLys: 0.411 ± 0.138
1.82TrpLeu: 1.82 ± 0.301
0.235TrpMet: 0.235 ± 0.097
0.822TrpAsn: 0.822 ± 0.273
0.646TrpPro: 0.646 ± 0.172
0.528TrpGln: 0.528 ± 0.161
1.291TrpArg: 1.291 ± 0.326
0.822TrpSer: 0.822 ± 0.21
0.881TrpThr: 0.881 ± 0.255
1.468TrpVal: 1.468 ± 0.279
0.47TrpTrp: 0.47 ± 0.196
0.822TrpTyr: 0.822 ± 0.244
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.407TyrAla: 2.407 ± 0.393
0.822TyrCys: 0.822 ± 0.253
2.348TyrAsp: 2.348 ± 0.402
1.82TyrGlu: 1.82 ± 0.283
1.174TyrPhe: 1.174 ± 0.252
2.407TyrGly: 2.407 ± 0.362
1.115TyrHis: 1.115 ± 0.274
1.996TyrIle: 1.996 ± 0.243
1.702TyrLys: 1.702 ± 0.391
2.466TyrLeu: 2.466 ± 0.378
0.822TyrMet: 0.822 ± 0.186
2.466TyrAsn: 2.466 ± 0.445
1.702TyrPro: 1.702 ± 0.336
1.174TyrGln: 1.174 ± 0.291
1.82TyrArg: 1.82 ± 0.412
1.291TyrSer: 1.291 ± 0.258
2.759TyrThr: 2.759 ± 0.381
2.583TyrVal: 2.583 ± 0.381
0.528TyrTrp: 0.528 ± 0.158
0.763TyrTyr: 0.763 ± 0.274
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (17036 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski