Paenibacillus antarcticus
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4663 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A162QGE7|A0A162QGE7_9BACL ABC transporter ATP-binding protein OS=Paenibacillus antarcticus OX=253703 GN=PBAT_01455 PE=4 SV=1
MM1 pKa = 7.52 NYY3 pKa = 9.68 IDD5 pKa = 4.5 IEE7 pKa = 4.51 KK8 pKa = 10.78 NLIPYY13 pKa = 10.18 RR14 pKa = 11.84 FDD16 pKa = 3.12 VSLAEE21 pKa = 3.92 EE22 pKa = 4.54 LFTFEE27 pKa = 4.08 VNYY30 pKa = 11.15 NEE32 pKa = 5.59 DD33 pKa = 3.44 YY34 pKa = 11.62 DD35 pKa = 4.56 FFTVDD40 pKa = 3.98 LEE42 pKa = 4.35 RR43 pKa = 11.84 DD44 pKa = 3.61 GEE46 pKa = 4.21 ILVVGEE52 pKa = 3.95 KK53 pKa = 10.46 LIYY56 pKa = 10.64 AMPLFYY62 pKa = 10.98 DD63 pKa = 3.89 VMDD66 pKa = 3.66 HH67 pKa = 7.03 RR68 pKa = 11.84 FPKK71 pKa = 10.21 ISIIPYY77 pKa = 9.83 DD78 pKa = 3.77 EE79 pKa = 4.76 SEE81 pKa = 4.13 NSIDD85 pKa = 4.86 VIWEE89 pKa = 4.04 TLSEE93 pKa = 4.55 SVFLYY98 pKa = 11.0 VIDD101 pKa = 4.54 NGEE104 pKa = 4.13 DD105 pKa = 3.44 TDD107 pKa = 4.03 GG108 pKa = 3.93
Molecular weight: 12.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.719
IPC_protein 3.681
Toseland 3.478
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.592
Rodwell 3.516
Grimsley 3.389
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 3.973
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.795
Patrickios 0.896
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A168Q9X3|A0A168Q9X3_9BACL Glycosyl hydrolase family 31 OS=Paenibacillus antarcticus OX=253703 GN=PBAT_04755 PE=3 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 PTFKK6 pKa = 10.84 PNVSKK11 pKa = 10.73 RR12 pKa = 11.84 AKK14 pKa = 8.72 VHH16 pKa = 5.43 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.87 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.48 GRR39 pKa = 11.84 KK40 pKa = 8.72 ILSAA44 pKa = 4.02
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.676
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.349
Sillero 12.866
Patrickios 12.398
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4663
0
4663
1491509
26
4467
319.9
35.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.871 ± 0.04
0.75 ± 0.01
5.282 ± 0.029
6.65 ± 0.043
4.146 ± 0.026
7.005 ± 0.035
2.109 ± 0.02
7.834 ± 0.038
5.779 ± 0.035
9.847 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.006 ± 0.017
4.498 ± 0.028
3.634 ± 0.019
3.881 ± 0.019
4.342 ± 0.027
6.779 ± 0.032
5.71 ± 0.033
7.144 ± 0.029
1.17 ± 0.012
3.564 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here