Nigrospora oryzae victorivirus 1
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S3IRQ6|A0A0S3IRQ6_9VIRU RNA-directed RNA polymerase OS=Nigrospora oryzae victorivirus 1 OX=1765736 GN=RdRp PE=3 SV=1
MM1 pKa = 7.73 ASTANFQVSVTSLLTGAVSGTSGGLIQTDD30 pKa = 3.72 GTFRR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 9.89 RR37 pKa = 11.84 SGLSIGVQEE46 pKa = 4.7 HH47 pKa = 6.07 GVLNYY52 pKa = 9.79 SRR54 pKa = 11.84 RR55 pKa = 11.84 SIFYY59 pKa = 9.59 EE60 pKa = 3.49 VGRR63 pKa = 11.84 RR64 pKa = 11.84 YY65 pKa = 10.5 GRR67 pKa = 11.84 LTDD70 pKa = 3.55 ALAYY74 pKa = 10.33 QKK76 pKa = 11.19 GDD78 pKa = 3.79 DD79 pKa = 4.32 EE80 pKa = 4.79 GLPLIDD86 pKa = 4.55 ASVPINPAQAANFEE100 pKa = 4.05 GWARR104 pKa = 11.84 KK105 pKa = 9.44 YY106 pKa = 11.34 SNFSPQWATMDD117 pKa = 3.85 LAGLAEE123 pKa = 4.2 RR124 pKa = 11.84 LAKK127 pKa = 10.19 GVAAQSVYY135 pKa = 11.25 GGVTTTNLRR144 pKa = 11.84 GGQPVRR150 pKa = 11.84 VVALGTLDD158 pKa = 3.82 SPQTASTNSIFIPRR172 pKa = 11.84 TVDD175 pKa = 2.78 TTTSDD180 pKa = 2.93 NTFAVLAAAANGEE193 pKa = 4.38 GATVTTDD200 pKa = 3.42 VVRR203 pKa = 11.84 LDD205 pKa = 3.42 AATNQPVIPAVDD217 pKa = 4.79 GYY219 pKa = 11.65 AFATACVEE227 pKa = 3.8 ALRR230 pKa = 11.84 IVGANLEE237 pKa = 4.16 EE238 pKa = 4.95 SGAGDD243 pKa = 3.32 VFAYY247 pKa = 10.22 AVARR251 pKa = 11.84 GIHH254 pKa = 6.41 AIVSVVAHH262 pKa = 6.09 TDD264 pKa = 2.83 EE265 pKa = 4.95 GGWMRR270 pKa = 11.84 NAFRR274 pKa = 11.84 SCTFRR279 pKa = 11.84 VPYY282 pKa = 10.55 GGINSALSQYY292 pKa = 9.81 PQLPPLAGSSAASVSSWVDD311 pKa = 3.28 AVALKK316 pKa = 9.07 TAAAVAHH323 pKa = 6.88 CDD325 pKa = 3.35 PCVTATGGLYY335 pKa = 7.99 PTVFTSSSGQVGAAGTAGDD354 pKa = 4.82 DD355 pKa = 3.79 PTNADD360 pKa = 3.52 AQDD363 pKa = 3.5 VARR366 pKa = 11.84 QLAADD371 pKa = 4.88 FGRR374 pKa = 11.84 FAPTYY379 pKa = 7.99 ITALRR384 pKa = 11.84 RR385 pKa = 11.84 IFGLTSDD392 pKa = 3.89 SGVAAMCFSTAARR405 pKa = 11.84 QALFGPPDD413 pKa = 3.04 RR414 pKa = 11.84 HH415 pKa = 6.24 LKK417 pKa = 9.46 YY418 pKa = 9.72 KK419 pKa = 9.89 TVAPYY424 pKa = 9.78 FWIEE428 pKa = 3.9 PTSLIPASAFGTTAEE443 pKa = 4.3 SAGFGALTTAGIEE456 pKa = 4.03 TTIPCFEE463 pKa = 4.28 RR464 pKa = 11.84 VRR466 pKa = 11.84 EE467 pKa = 3.99 LDD469 pKa = 2.82 HH470 pKa = 6.78 GRR472 pKa = 11.84 NANFSTIAFKK482 pKa = 10.48 MRR484 pKa = 11.84 TARR487 pKa = 11.84 TSGLVAAYY495 pKa = 9.86 AGAPAALAGLKK506 pKa = 10.08 LYY508 pKa = 10.74 QFDD511 pKa = 4.04 EE512 pKa = 5.08 DD513 pKa = 4.35 SVALAGDD520 pKa = 3.59 QGPTPGDD527 pKa = 3.14 VPMKK531 pKa = 10.41 HH532 pKa = 6.08 SQADD536 pKa = 3.72 PLSSYY541 pKa = 10.7 LWTRR545 pKa = 11.84 GQSPIPAPAEE555 pKa = 4.02 FINIQGAYY563 pKa = 7.45 AAKK566 pKa = 10.26 YY567 pKa = 10.18 KK568 pKa = 10.5 IVDD571 pKa = 3.34 WDD573 pKa = 3.71 DD574 pKa = 3.81 DD575 pKa = 4.54 FNGRR579 pKa = 11.84 LGDD582 pKa = 3.68 LPEE585 pKa = 4.69 AFEE588 pKa = 4.53 LEE590 pKa = 4.32 NYY592 pKa = 9.27 GIRR595 pKa = 11.84 WRR597 pKa = 11.84 VTVPTALPSGASNAGDD613 pKa = 3.4 TGAKK617 pKa = 9.24 RR618 pKa = 11.84 ARR620 pKa = 11.84 TRR622 pKa = 11.84 AAIALAQANIRR633 pKa = 11.84 NRR635 pKa = 11.84 GFGEE639 pKa = 4.07 ANSPVISVSNVPPSFDD655 pKa = 3.54 DD656 pKa = 3.7 TPPPMMAAASGAVHH670 pKa = 7.23 DD671 pKa = 4.72 AANRR675 pKa = 11.84 TIPEE679 pKa = 4.2 YY680 pKa = 11.32 AEE682 pKa = 4.25 GAATAGAPPVMGAPLGPIPHH702 pKa = 6.41 HH703 pKa = 5.9 QPLRR707 pKa = 11.84 GAPYY711 pKa = 8.7 PRR713 pKa = 11.84 QGGGQVGGAGLPPNPPLPGNNPPPPPPTGPSNPPTNPDD751 pKa = 3.51 LDD753 pKa = 4.21 YY754 pKa = 11.33 QPDD757 pKa = 3.82 PAGPEE762 pKa = 3.81 AAHH765 pKa = 6.64 APPAGQVV772 pKa = 2.93
Molecular weight: 79.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.334
IPC2_protein 5.347
IPC_protein 5.347
Toseland 5.537
ProMoST 5.601
Dawson 5.474
Bjellqvist 5.55
Wikipedia 5.436
Rodwell 5.41
Grimsley 5.575
Solomon 5.474
Lehninger 5.448
Nozaki 5.664
DTASelect 5.868
Thurlkill 5.664
EMBOSS 5.652
Sillero 5.753
Patrickios 3.986
IPC_peptide 5.486
IPC2_peptide 5.753
IPC2.peptide.svr19 5.718
Protein with the highest isoelectric point:
>tr|A0A0S3IRQ6|A0A0S3IRQ6_9VIRU RNA-directed RNA polymerase OS=Nigrospora oryzae victorivirus 1 OX=1765736 GN=RdRp PE=3 SV=1
MM1 pKa = 7.2 GQRR4 pKa = 11.84 ATEE7 pKa = 4.2 RR8 pKa = 11.84 GNDD11 pKa = 3.82 PPSLGQALEE20 pKa = 4.16 RR21 pKa = 11.84 VARR24 pKa = 11.84 RR25 pKa = 11.84 MGITAKK31 pKa = 10.64 LDD33 pKa = 3.3 MPFADD38 pKa = 4.16 QISFIYY44 pKa = 9.76 RR45 pKa = 11.84 PQWDD49 pKa = 3.9 GRR51 pKa = 11.84 PVPRR55 pKa = 11.84 LARR58 pKa = 11.84 AAVSYY63 pKa = 8.39 LYY65 pKa = 10.6 CDD67 pKa = 3.6 VPVQVPLEE75 pKa = 4.3 KK76 pKa = 10.51 DD77 pKa = 3.04 DD78 pKa = 3.78 LTRR81 pKa = 11.84 LLGYY85 pKa = 10.18 LCEE88 pKa = 4.59 HH89 pKa = 6.8 LTMPPSLDD97 pKa = 3.23 RR98 pKa = 11.84 QTWVTDD104 pKa = 3.34 KK105 pKa = 10.63 KK106 pKa = 10.74 RR107 pKa = 11.84 AMEE110 pKa = 4.46 AFPPKK115 pKa = 9.44 RR116 pKa = 11.84 RR117 pKa = 11.84 AMALTKK123 pKa = 10.86 ANIFLDD129 pKa = 4.14 EE130 pKa = 4.18 VARR133 pKa = 11.84 DD134 pKa = 3.75 LDD136 pKa = 3.24 KK137 pKa = 11.55 HH138 pKa = 5.23 MPARR142 pKa = 11.84 LLALLPYY149 pKa = 9.89 LGKK152 pKa = 10.54 LRR154 pKa = 11.84 GVGATHH160 pKa = 7.06 DD161 pKa = 3.52 QATAFLLYY169 pKa = 10.65 SSLLSDD175 pKa = 4.34 HH176 pKa = 6.96 CPLAAEE182 pKa = 4.28 WGLFALTQPKK192 pKa = 8.85 EE193 pKa = 4.08 AKK195 pKa = 9.59 EE196 pKa = 3.81 VSNFLKK202 pKa = 10.81 AVGANASSYY211 pKa = 10.86 GALLVEE217 pKa = 4.54 TDD219 pKa = 3.55 TLLGRR224 pKa = 11.84 DD225 pKa = 3.72 TPGTDD230 pKa = 3.07 LVADD234 pKa = 3.56 SRR236 pKa = 11.84 RR237 pKa = 11.84 RR238 pKa = 11.84 CDD240 pKa = 3.54 LEE242 pKa = 3.89 LARR245 pKa = 11.84 EE246 pKa = 4.0 EE247 pKa = 4.16 VLAEE251 pKa = 4.17 FDD253 pKa = 4.71 DD254 pKa = 4.11 SALRR258 pKa = 11.84 SAVRR262 pKa = 11.84 RR263 pKa = 11.84 VLRR266 pKa = 11.84 HH267 pKa = 5.33 EE268 pKa = 4.29 LRR270 pKa = 11.84 SEE272 pKa = 3.82 HH273 pKa = 5.41 GSYY276 pKa = 10.31 RR277 pKa = 11.84 IEE279 pKa = 4.28 YY280 pKa = 7.02 PTQEE284 pKa = 4.28 DD285 pKa = 3.6 HH286 pKa = 5.84 WTSRR290 pKa = 11.84 WSWAVNGSHH299 pKa = 6.6 SALLAGEE306 pKa = 4.25 FPVPNEE312 pKa = 4.04 VKK314 pKa = 10.15 RR315 pKa = 11.84 QVHH318 pKa = 5.55 KK319 pKa = 9.87 LHH321 pKa = 6.35 RR322 pKa = 11.84 RR323 pKa = 11.84 AWLEE327 pKa = 3.89 TIEE330 pKa = 4.46 RR331 pKa = 11.84 NPCIGWDD338 pKa = 3.02 GKK340 pKa = 8.75 TFVSCAPKK348 pKa = 10.27 PEE350 pKa = 4.39 CGKK353 pKa = 9.92 IRR355 pKa = 11.84 AIYY358 pKa = 10.33 ACDD361 pKa = 3.68 TINYY365 pKa = 8.97 LAFEE369 pKa = 4.3 HH370 pKa = 6.71 LMAGVEE376 pKa = 4.24 SRR378 pKa = 11.84 WLGKK382 pKa = 10.09 RR383 pKa = 11.84 VILNPGRR390 pKa = 11.84 GGHH393 pKa = 6.5 LGIAEE398 pKa = 3.99 RR399 pKa = 11.84 VAACRR404 pKa = 11.84 SRR406 pKa = 11.84 SGISAMLDD414 pKa = 3.2 YY415 pKa = 11.48 DD416 pKa = 4.67 DD417 pKa = 5.98 FNSHH421 pKa = 5.95 HH422 pKa = 5.91 TTKK425 pKa = 10.93 AMQILIEE432 pKa = 4.11 EE433 pKa = 4.4 TAALTGYY440 pKa = 9.61 PPEE443 pKa = 4.15 LTKK446 pKa = 10.66 PLLDD450 pKa = 3.66 SFEE453 pKa = 5.25 KK454 pKa = 10.67 EE455 pKa = 4.92 DD456 pKa = 3.78 IYY458 pKa = 11.45 LDD460 pKa = 3.68 GKK462 pKa = 10.81 LVGRR466 pKa = 11.84 VKK468 pKa = 9.49 GTLMSGHH475 pKa = 6.99 RR476 pKa = 11.84 CTTYY480 pKa = 10.84 VNSVLNMAYY489 pKa = 10.81 LMVVLGEE496 pKa = 4.07 DD497 pKa = 2.9 WVMEE501 pKa = 4.17 RR502 pKa = 11.84 QSLHH506 pKa = 7.11 VGDD509 pKa = 4.61 DD510 pKa = 3.37 VYY512 pKa = 11.02 MGLKK516 pKa = 10.45 NYY518 pKa = 10.77 GEE520 pKa = 4.51 TGWMISKK527 pKa = 10.28 IMGSRR532 pKa = 11.84 LRR534 pKa = 11.84 MNRR537 pKa = 11.84 RR538 pKa = 11.84 KK539 pKa = 10.03 QSVGHH544 pKa = 6.03 VSTEE548 pKa = 3.89 FLRR551 pKa = 11.84 VASDD555 pKa = 3.18 ARR557 pKa = 11.84 DD558 pKa = 3.38 SYY560 pKa = 11.92 GYY562 pKa = 10.14 LARR565 pKa = 11.84 AAANLIAGNWYY576 pKa = 10.11 SDD578 pKa = 3.18 KK579 pKa = 10.97 LLNPSEE585 pKa = 4.21 GLITMIQGARR595 pKa = 11.84 TLANRR600 pKa = 11.84 ARR602 pKa = 11.84 NGNAPLLLEE611 pKa = 4.3 SAVKK615 pKa = 10.17 RR616 pKa = 11.84 VLGADD621 pKa = 3.89 CPDD624 pKa = 4.23 DD625 pKa = 3.39 ATLRR629 pKa = 11.84 HH630 pKa = 6.14 MLTGSAAVNDD640 pKa = 4.53 GPQYY644 pKa = 10.53 LSSGRR649 pKa = 11.84 HH650 pKa = 3.35 RR651 pKa = 11.84 RR652 pKa = 11.84 VRR654 pKa = 11.84 TSVVFEE660 pKa = 4.24 VVDD663 pKa = 3.58 TTGYY667 pKa = 8.73 ATLPDD672 pKa = 4.34 LASKK676 pKa = 10.78 AYY678 pKa = 9.16 LAKK681 pKa = 10.18 HH682 pKa = 6.13 ASPLEE687 pKa = 3.99 IATLTSSGIDD697 pKa = 3.39 PLPTMRR703 pKa = 11.84 KK704 pKa = 9.3 SSWTKK709 pKa = 10.73 SLALSDD715 pKa = 4.33 RR716 pKa = 11.84 KK717 pKa = 10.61 FEE719 pKa = 4.47 TIVFKK724 pKa = 10.97 PMEE727 pKa = 4.0 QWPAVGSVQAEE738 pKa = 3.84 HH739 pKa = 7.32 LLRR742 pKa = 11.84 EE743 pKa = 4.39 RR744 pKa = 11.84 KK745 pKa = 8.56 PHH747 pKa = 6.0 GVLLKK752 pKa = 10.9 YY753 pKa = 10.14 PLLVLMRR760 pKa = 11.84 DD761 pKa = 4.47 RR762 pKa = 11.84 IPEE765 pKa = 3.91 PVLRR769 pKa = 11.84 VAVAAAGGRR778 pKa = 11.84 DD779 pKa = 3.75 YY780 pKa = 11.76 VDD782 pKa = 5.79 DD783 pKa = 4.54 ISLEE787 pKa = 3.89 AWGDD791 pKa = 3.57 YY792 pKa = 11.05 SNPTIVNTVLSYY804 pKa = 11.58 SDD806 pKa = 4.55 ASMLSKK812 pKa = 10.39 RR813 pKa = 11.84 ASVSVLTSRR822 pKa = 11.84 RR823 pKa = 11.84 RR824 pKa = 11.84 CYY826 pKa = 10.4 VV827 pKa = 2.75
Molecular weight: 92.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.013
IPC2_protein 8.156
IPC_protein 8.024
Toseland 8.068
ProMoST 8.551
Dawson 8.799
Bjellqvist 8.946
Wikipedia 8.843
Rodwell 8.858
Grimsley 8.156
Solomon 8.873
Lehninger 8.843
Nozaki 9.048
DTASelect 8.756
Thurlkill 8.814
EMBOSS 8.96
Sillero 9.063
Patrickios 4.558
IPC_peptide 8.858
IPC2_peptide 7.805
IPC2.peptide.svr19 7.849
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1599
772
827
799.5
86.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.633 ± 2.037
1.126 ± 0.233
5.691 ± 0.255
4.315 ± 0.808
2.877 ± 0.589
8.38 ± 1.241
2.064 ± 0.428
3.502 ± 0.17
3.002 ± 0.97
9.193 ± 1.907
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.064 ± 0.602
3.315 ± 0.383
6.942 ± 1.337
2.752 ± 0.5
7.067 ± 1.09
6.942 ± 0.225
6.504 ± 0.763
6.879 ± 0.096
1.438 ± 0.269
3.315 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here