Mucilaginibacter mali
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5033 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D4PUU0|A0A7D4PUU0_9SPHI Phosphatase PAP2 family protein OS=Mucilaginibacter mali OX=2740462 GN=HQ865_12605 PE=4 SV=1
MM1 pKa = 7.52 NIIPFQSVGDD11 pKa = 3.9 LNFTDD16 pKa = 3.62 EE17 pKa = 4.25 RR18 pKa = 11.84 HH19 pKa = 5.06 VLRR22 pKa = 11.84 GKK24 pKa = 10.08 IDD26 pKa = 3.61 GEE28 pKa = 4.33 YY29 pKa = 9.6 QPGMNEE35 pKa = 4.16 FEE37 pKa = 4.45 GHH39 pKa = 4.51 TEE41 pKa = 4.02 YY42 pKa = 11.51 YY43 pKa = 10.96 DD44 pKa = 3.57 FFPEE48 pKa = 3.55 VDD50 pKa = 3.13 MLIYY54 pKa = 10.57 YY55 pKa = 10.16 DD56 pKa = 4.46 ADD58 pKa = 3.48 DD59 pKa = 3.95 RR60 pKa = 11.84 VNAFEE65 pKa = 5.22 FFSQGPEE72 pKa = 3.78 YY73 pKa = 10.64 RR74 pKa = 11.84 DD75 pKa = 2.95 IDD77 pKa = 4.31 LLSEE81 pKa = 4.52 TYY83 pKa = 11.24 ADD85 pKa = 3.82 LLKK88 pKa = 10.67 LFKK91 pKa = 11.15 VFDD94 pKa = 4.15 PEE96 pKa = 5.64 LIIEE100 pKa = 4.28 EE101 pKa = 4.86 DD102 pKa = 3.42 NFEE105 pKa = 4.12 SAKK108 pKa = 10.86 AGIVVNATEE117 pKa = 4.71 PDD119 pKa = 3.92 DD120 pKa = 4.96 LPEE123 pKa = 4.23 SVLVYY128 pKa = 10.55 RR129 pKa = 11.84 EE130 pKa = 4.38 GYY132 pKa = 9.87 YY133 pKa = 10.23 EE134 pKa = 3.84
Molecular weight: 15.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.656
ProMoST 3.973
Dawson 3.834
Bjellqvist 4.024
Wikipedia 3.732
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.77
Nozaki 3.948
DTASelect 4.126
Thurlkill 3.694
EMBOSS 3.745
Sillero 3.973
Patrickios 1.875
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.887
Protein with the highest isoelectric point:
>tr|A0A7D4ULG6|A0A7D4ULG6_9SPHI 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Mucilaginibacter mali OX=2740462 GN=fabF PE=3 SV=1
MM1 pKa = 7.62 KK2 pKa = 9.93 IARR5 pKa = 11.84 ILIIAMLMATVTVSVNAGTVTAPASVVWQVKK36 pKa = 9.64 LPPPPPHH43 pKa = 6.92 PGKK46 pKa = 9.89 PPVVVNHH53 pKa = 5.48 RR54 pKa = 11.84 TVTVRR59 pKa = 11.84 KK60 pKa = 8.64 PPAHH64 pKa = 6.52 PRR66 pKa = 11.84 PKK68 pKa = 9.79 RR69 pKa = 11.84 LHH71 pKa = 6.09 IKK73 pKa = 9.91 LPHH76 pKa = 6.17 VKK78 pKa = 10.17 LPPPPPHH85 pKa = 6.99 PPLL88 pKa = 5.16
Molecular weight: 9.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.906
IPC_protein 12.442
Toseland 12.632
ProMoST 13.1
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.086
Rodwell 12.544
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.115
Sillero 12.632
Patrickios 12.281
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.991
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5033
0
5033
1747214
24
2992
347.2
38.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.111 ± 0.032
0.804 ± 0.011
5.599 ± 0.025
4.99 ± 0.036
4.637 ± 0.025
7.143 ± 0.035
1.948 ± 0.018
6.933 ± 0.029
6.772 ± 0.034
9.186 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.427 ± 0.018
5.569 ± 0.032
4.049 ± 0.022
3.826 ± 0.025
3.969 ± 0.022
5.967 ± 0.034
6.138 ± 0.043
6.437 ± 0.025
1.24 ± 0.014
4.257 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here