Salobo virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Salobo phlebovirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1ST85|I1ST85_9VIRU Nucleoprotein OS=Salobo virus OX=427316 PE=3 SV=1
MM1 pKa = 7.24NFLYY5 pKa = 10.12EE6 pKa = 4.13MPVILSYY13 pKa = 11.58GGMNTPEE20 pKa = 4.82AEE22 pKa = 4.08VGYY25 pKa = 10.3YY26 pKa = 9.73PYY28 pKa = 11.25AGFTTYY34 pKa = 10.05RR35 pKa = 11.84VSVHH39 pKa = 6.46NEE41 pKa = 3.24MEE43 pKa = 4.5IPVGTYY49 pKa = 9.44RR50 pKa = 11.84QVMDD54 pKa = 3.84HH55 pKa = 7.17RR56 pKa = 11.84DD57 pKa = 3.55NFEE60 pKa = 3.64DD61 pKa = 5.37FYY63 pKa = 11.38RR64 pKa = 11.84VGHH67 pKa = 6.29FPFRR71 pKa = 11.84WGPGCFNSRR80 pKa = 11.84VDD82 pKa = 3.91SQTCPDD88 pKa = 4.55FDD90 pKa = 4.16TMLKK94 pKa = 11.14DD95 pKa = 3.05MMLFPRR101 pKa = 11.84EE102 pKa = 3.76AYY104 pKa = 8.4TRR106 pKa = 11.84SFLPNISEE114 pKa = 4.66AISWPLGYY122 pKa = 9.4PCSEE126 pKa = 5.06FIQLSNEE133 pKa = 3.72ARR135 pKa = 11.84SDD137 pKa = 3.3LSLRR141 pKa = 11.84SWKK144 pKa = 10.17SAAATCILRR153 pKa = 11.84MNPWVSTFDD162 pKa = 3.38QAFVEE167 pKa = 4.13AHH169 pKa = 5.8KK170 pKa = 10.63MVVAEE175 pKa = 4.19ANNRR179 pKa = 11.84GLSRR183 pKa = 11.84MLFPGFDD190 pKa = 3.02IVKK193 pKa = 9.72EE194 pKa = 4.02VAIVQLVRR202 pKa = 11.84AMNALDD208 pKa = 3.61IDD210 pKa = 4.04MTYY213 pKa = 11.12SRR215 pKa = 11.84IPTVLTNMLEE225 pKa = 3.77KK226 pKa = 10.62HH227 pKa = 5.7KK228 pKa = 11.05AVFEE232 pKa = 4.18NEE234 pKa = 3.85NPDD237 pKa = 3.86LLGNRR242 pKa = 11.84KK243 pKa = 8.99WIPSEE248 pKa = 3.96DD249 pKa = 3.09TWFDD253 pKa = 3.22GLNYY257 pKa = 10.37NGEE260 pKa = 4.41SEE262 pKa = 4.45FDD264 pKa = 3.72SSDD267 pKa = 3.06SDD269 pKa = 3.79AAA271 pKa = 3.89

Molecular weight: 31.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1ST86|I1ST86_9VIRU Replicase OS=Salobo virus OX=427316 PE=4 SV=1
MM1 pKa = 7.74ADD3 pKa = 3.42YY4 pKa = 11.31ARR6 pKa = 11.84IAVEE10 pKa = 4.43FSGEE14 pKa = 4.15AINLAEE20 pKa = 4.05IQGWVTDD27 pKa = 3.89FAYY30 pKa = 10.45QGFDD34 pKa = 2.65ARR36 pKa = 11.84RR37 pKa = 11.84IVEE40 pKa = 4.28LVQQKK45 pKa = 10.56GGAGWKK51 pKa = 10.11DD52 pKa = 3.68DD53 pKa = 3.81VKK55 pKa = 11.2MMIVLCLTRR64 pKa = 11.84GNKK67 pKa = 5.97PTKK70 pKa = 9.31MVEE73 pKa = 3.9KK74 pKa = 9.51MSPEE78 pKa = 3.86GKK80 pKa = 10.01VKK82 pKa = 10.44VNRR85 pKa = 11.84LISTYY90 pKa = 9.01GLKK93 pKa = 10.39SGNPGRR99 pKa = 11.84DD100 pKa = 3.8DD101 pKa = 3.22ITLSRR106 pKa = 11.84VAAAFAGWTCQALNVLHH123 pKa = 7.33PYY125 pKa = 10.45LPVSGTTMDD134 pKa = 5.36AISPNYY140 pKa = 8.24PRR142 pKa = 11.84AMMHH146 pKa = 6.41PCFAGLVDD154 pKa = 3.46QTIPTEE160 pKa = 4.1YY161 pKa = 10.39CQTIVDD167 pKa = 3.85AMSVFLIQFSRR178 pKa = 11.84TINKK182 pKa = 9.02NLRR185 pKa = 11.84GQPKK189 pKa = 9.0EE190 pKa = 3.86VVIEE194 pKa = 4.2SFIQPMQAAMSSSFIAPAEE213 pKa = 4.02RR214 pKa = 11.84RR215 pKa = 11.84KK216 pKa = 10.84LMIALGIVDD225 pKa = 5.03ANGKK229 pKa = 9.38PSANVAAAAAVFPRR243 pKa = 11.84LLL245 pKa = 4.3

Molecular weight: 26.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3961

245

2084

990.3

111.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.706 ± 1.1

2.626 ± 0.757

5.327 ± 0.554

7.195 ± 0.593

4.342 ± 0.766

6.438 ± 0.604

2.222 ± 0.277

5.933 ± 0.271

6.741 ± 0.872

8.306 ± 0.458

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.509 ± 0.434

4.191 ± 0.387

4.342 ± 0.407

2.954 ± 0.231

5.15 ± 0.137

8.811 ± 0.721

5.251 ± 0.145

7.069 ± 0.31

1.288 ± 0.15

2.6 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski