Hepatospora eriocheir
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2687 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X0Q8R8|A0A1X0Q8R8_9MICR MYB OS=Hepatospora eriocheir OX=1081669 GN=MYB PE=4 SV=1
MM1 pKa = 7.88 GDD3 pKa = 4.24 LGIDD7 pKa = 5.27 DD8 pKa = 6.24 IIDD11 pKa = 3.52 QNNASSDD18 pKa = 3.71 CDD20 pKa = 3.56 NCITKK25 pKa = 10.4 CDD27 pKa = 4.72 DD28 pKa = 3.52 IEE30 pKa = 4.47 VEE32 pKa = 4.18 CGDD35 pKa = 4.41 IEE37 pKa = 4.64 VEE39 pKa = 4.13 CGDD42 pKa = 3.97 IEE44 pKa = 5.2 KK45 pKa = 10.74 EE46 pKa = 4.13 CGDD49 pKa = 4.24 LKK51 pKa = 11.24 VEE53 pKa = 4.39 CGDD56 pKa = 4.29 LKK58 pKa = 11.24 VEE60 pKa = 4.61 CGDD63 pKa = 3.81 IKK65 pKa = 11.06 KK66 pKa = 10.45 EE67 pKa = 3.93 CDD69 pKa = 3.55 DD70 pKa = 4.83 LKK72 pKa = 11.44 VEE74 pKa = 4.48 CGDD77 pKa = 4.29 LKK79 pKa = 11.26 VEE81 pKa = 4.39 CGDD84 pKa = 4.84 LKK86 pKa = 11.22 VEE88 pKa = 4.34 CDD90 pKa = 4.44 DD91 pKa = 5.89 IEE93 pKa = 4.79 VEE95 pKa = 4.2 CDD97 pKa = 3.45 DD98 pKa = 6.0 LKK100 pKa = 11.56 VEE102 pKa = 4.26 CDD104 pKa = 3.53 NLKK107 pKa = 10.46 VEE109 pKa = 4.55 CDD111 pKa = 3.73 DD112 pKa = 6.08 LKK114 pKa = 11.51 VEE116 pKa = 4.26 CDD118 pKa = 3.53 NLKK121 pKa = 10.46 VEE123 pKa = 4.88 CDD125 pKa = 3.7 DD126 pKa = 5.99 LKK128 pKa = 11.47 VKK130 pKa = 10.74 CDD132 pKa = 4.73 DD133 pKa = 4.41 IEE135 pKa = 4.44 KK136 pKa = 10.04 EE137 pKa = 3.93 CDD139 pKa = 4.01 DD140 pKa = 4.96 IEE142 pKa = 4.53 VEE144 pKa = 4.21 CGDD147 pKa = 4.37 IEE149 pKa = 4.85 VEE151 pKa = 4.44 CVDD154 pKa = 4.06 LKK156 pKa = 11.11 KK157 pKa = 10.96 SVVILL162 pKa = 3.77
Molecular weight: 17.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.427
Solomon 3.719
Lehninger 3.668
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.859
Patrickios 0.973
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|A0A1X0Q6M7|A0A1X0Q6M7_9MICR SC61A OS=Hepatospora eriocheir OX=1081669 GN=SC61A PE=4 SV=1
MM1 pKa = 7.15 IQLLEE6 pKa = 4.27 KK7 pKa = 10.76 YY8 pKa = 9.58 ISKK11 pKa = 10.88 LNATKK16 pKa = 10.93 VNFTDD21 pKa = 3.82 SNMLKK26 pKa = 8.68 TCVKK30 pKa = 10.56 GIFVSHH36 pKa = 6.36 RR37 pKa = 11.84 RR38 pKa = 11.84 GLRR41 pKa = 11.84 RR42 pKa = 11.84 LRR44 pKa = 11.84 PTQSIIKK51 pKa = 9.09 INNVTDD57 pKa = 3.58 SEE59 pKa = 4.49 TASAYY64 pKa = 9.87 INNAIMFCYY73 pKa = 9.96 RR74 pKa = 11.84 NKK76 pKa = 10.39 DD77 pKa = 3.17 GKK79 pKa = 8.58 TIRR82 pKa = 11.84 INGFIRR88 pKa = 11.84 ATHH91 pKa = 5.7 GNKK94 pKa = 9.89 GAVRR98 pKa = 11.84 ATFEE102 pKa = 5.07 RR103 pKa = 11.84 NLNPQWIGRR112 pKa = 11.84 EE113 pKa = 4.12 VFIKK117 pKa = 10.44 LYY119 pKa = 10.5 KK120 pKa = 9.87 HH121 pKa = 6.17
Molecular weight: 13.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.838
IPC_protein 10.423
Toseland 10.804
ProMoST 10.394
Dawson 10.891
Bjellqvist 10.54
Wikipedia 11.052
Rodwell 11.272
Grimsley 10.921
Solomon 10.965
Lehninger 10.95
Nozaki 10.774
DTASelect 10.54
Thurlkill 10.789
EMBOSS 11.184
Sillero 10.804
Patrickios 11.008
IPC_peptide 10.979
IPC2_peptide 9.414
IPC2.peptide.svr19 8.533
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2687
0
2687
627558
33
2666
233.6
27.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.535 ± 0.031
1.701 ± 0.022
5.805 ± 0.035
7.387 ± 0.054
5.977 ± 0.047
3.309 ± 0.042
1.472 ± 0.016
10.042 ± 0.056
10.767 ± 0.067
9.989 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.151 ± 0.021
8.779 ± 0.062
2.183 ± 0.029
2.409 ± 0.027
3.339 ± 0.027
7.018 ± 0.044
4.551 ± 0.037
5.211 ± 0.038
0.457 ± 0.01
4.668 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here