Granulicella rosea
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4295 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239IYM9|A0A239IYM9_9BACT Isoamylase OS=Granulicella rosea OX=474952 GN=SAMN05421770_103335 PE=3 SV=1
MM1 pKa = 7.68 SLGILNNIAAIYY13 pKa = 8.99 AQNNLNQTQASLQTTLNQLSSGSRR37 pKa = 11.84 INSGADD43 pKa = 3.18 DD44 pKa = 4.1 AAGLAVVDD52 pKa = 4.15 GLGANIAALTQSSQNATDD70 pKa = 5.57 GIGLLQTADD79 pKa = 3.52 GALSQVTNLLDD90 pKa = 3.64 RR91 pKa = 11.84 AVTLSTEE98 pKa = 4.17 AANGTLNSNQVSSANQEE115 pKa = 3.94 YY116 pKa = 10.9 QNILNEE122 pKa = 4.19 IGDD125 pKa = 3.72 IGTTTNFNSNSVFSNIANTVFVSDD149 pKa = 4.03 GTGSGANVYY158 pKa = 10.72 SDD160 pKa = 3.04 IVGVLSKK167 pKa = 11.47 SNVGQSAPVGAGVALTSGTATTSSLTVGSSVTFSALTNYY206 pKa = 10.39 NDD208 pKa = 4.33 TIAGTLSVSVAGGTAINSTIATGTSLTDD236 pKa = 4.27 AVNQLNDD243 pKa = 3.31 QYY245 pKa = 11.53 NADD248 pKa = 3.44 GSGIVASIGSGNKK261 pKa = 9.45 LVLTGPEE268 pKa = 3.99 DD269 pKa = 4.41 AEE271 pKa = 4.21 NTTAGTNVLAVSTNTLEE288 pKa = 4.93 DD289 pKa = 3.81 YY290 pKa = 11.24 APAAAGATAGAGIDD304 pKa = 3.88 FTNSDD309 pKa = 4.02 VSTLTQSSAQTVLTTVTSAIAGVAYY334 pKa = 10.03 QRR336 pKa = 11.84 GIIGADD342 pKa = 2.97 INQLTAASNVASAEE356 pKa = 4.18 SEE358 pKa = 4.15 NLTSAQSSIQSTNYY372 pKa = 9.61 GQATSDD378 pKa = 3.47 LAKK381 pKa = 10.71 YY382 pKa = 9.63 QVLSQTGISALAQANSVQQEE402 pKa = 4.33 VLKK405 pKa = 10.92 LLQQ408 pKa = 3.71
Molecular weight: 40.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.541
IPC_protein 3.541
Toseland 3.325
ProMoST 3.732
Dawson 3.554
Bjellqvist 3.719
Wikipedia 3.516
Rodwell 3.376
Grimsley 3.236
Solomon 3.541
Lehninger 3.49
Nozaki 3.681
DTASelect 3.935
Thurlkill 3.389
EMBOSS 3.528
Sillero 3.668
Patrickios 0.896
IPC_peptide 3.528
IPC2_peptide 3.643
IPC2.peptide.svr19 3.68
Protein with the highest isoelectric point:
>tr|A0A239DWU5|A0A239DWU5_9BACT Cytidine deaminase OS=Granulicella rosea OX=474952 GN=SAMN05421770_101689 PE=3 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.02 RR4 pKa = 11.84 TFQPNRR10 pKa = 11.84 RR11 pKa = 11.84 HH12 pKa = 5.69 RR13 pKa = 11.84 AKK15 pKa = 9.55 THH17 pKa = 5.53 GFLSRR22 pKa = 11.84 MKK24 pKa = 8.98 TKK26 pKa = 10.79 AGAAVLNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.03 GRR40 pKa = 11.84 HH41 pKa = 5.72 KK42 pKa = 10.29 IAVSAGFRR50 pKa = 11.84 DD51 pKa = 3.66
Molecular weight: 5.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4295
0
4295
1567266
28
2848
364.9
39.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.702 ± 0.05
0.851 ± 0.012
5.017 ± 0.03
5.173 ± 0.046
3.738 ± 0.022
8.249 ± 0.044
2.183 ± 0.019
4.848 ± 0.027
3.535 ± 0.034
9.963 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.204 ± 0.021
3.325 ± 0.032
5.473 ± 0.029
3.698 ± 0.027
5.895 ± 0.049
6.253 ± 0.038
6.572 ± 0.061
7.227 ± 0.029
1.294 ± 0.016
2.8 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here