Curtobacterium sp. Leaf183

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Curtobacterium; unclassified Curtobacterium

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3232 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5RHS8|A0A0Q5RHS8_9MICO Sugar ABC transporter substrate-binding protein OS=Curtobacterium sp. Leaf183 OX=1736291 GN=ASG04_15885 PE=4 SV=1
MM1 pKa = 7.81SEE3 pKa = 4.23QLTNTYY9 pKa = 10.21GITGMTCGHH18 pKa = 6.58CVMSVNEE25 pKa = 4.29EE26 pKa = 3.87LAAVPGVTDD35 pKa = 2.96VTIDD39 pKa = 3.7LNVGGVSTARR49 pKa = 11.84VTSTRR54 pKa = 11.84DD55 pKa = 3.19LPQEE59 pKa = 4.03EE60 pKa = 4.37VSAAVEE66 pKa = 3.96EE67 pKa = 4.33AGYY70 pKa = 8.55TLVASS75 pKa = 4.51

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5RYE4|A0A0Q5RYE4_9MICO Puromycin resistance protein pur8 OS=Curtobacterium sp. Leaf183 OX=1736291 GN=ASG04_02510 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.52GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3232

0

3232

1037927

29

1933

321.1

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.558 ± 0.05

0.447 ± 0.01

6.715 ± 0.041

4.817 ± 0.047

3.029 ± 0.027

9.099 ± 0.039

2.07 ± 0.025

3.807 ± 0.029

1.845 ± 0.031

9.622 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.708 ± 0.015

1.798 ± 0.022

5.383 ± 0.026

2.983 ± 0.028

7.355 ± 0.053

5.632 ± 0.039

6.746 ± 0.044

10.031 ± 0.045

1.508 ± 0.02

1.847 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski