Psychrobacter piechaudii
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2327 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R4GDD8|A0A1R4GDD8_9GAMM Disulfide bond formation protein B OS=Psychrobacter piechaudii OX=1945521 GN=dsbB PE=3 SV=1
MM1 pKa = 6.61 TTITLTDD8 pKa = 3.23 SAANKK13 pKa = 8.76 VRR15 pKa = 11.84 KK16 pKa = 9.36 LRR18 pKa = 11.84 EE19 pKa = 3.83 EE20 pKa = 4.4 EE21 pKa = 4.07 GDD23 pKa = 3.52 QNLMLRR29 pKa = 11.84 VYY31 pKa = 8.97 VTGGGCSGFSYY42 pKa = 10.47 GFNFAEE48 pKa = 5.1 EE49 pKa = 4.29 EE50 pKa = 4.56 GEE52 pKa = 4.17 DD53 pKa = 3.58 DD54 pKa = 5.31 ASFEE58 pKa = 4.32 NEE60 pKa = 4.35 DD61 pKa = 3.2 VTLLVDD67 pKa = 3.69 SLSYY71 pKa = 10.75 QYY73 pKa = 11.26 LQGSTVDD80 pKa = 3.47 YY81 pKa = 11.18 TEE83 pKa = 4.21 GLEE86 pKa = 4.13 GARR89 pKa = 11.84 FVVTNPNATTTCGCGSSFSII109 pKa = 5.02
Molecular weight: 11.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.986
IPC_protein 3.884
Toseland 3.706
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.63
Solomon 3.834
Lehninger 3.783
Nozaki 3.973
DTASelect 4.113
Thurlkill 3.745
EMBOSS 3.757
Sillero 3.999
Patrickios 0.846
IPC_peptide 3.834
IPC2_peptide 3.986
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|A0A1R4GUM4|A0A1R4GUM4_9GAMM Corrinoid adenosyltransferase OS=Psychrobacter piechaudii OX=1945521 GN=A1232T_01420 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.52 RR12 pKa = 11.84 KK13 pKa = 8.26 RR14 pKa = 11.84 THH16 pKa = 5.92 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.32 KK26 pKa = 10.31 GRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.03 GRR39 pKa = 11.84 HH40 pKa = 5.34 RR41 pKa = 11.84 LTVV44 pKa = 3.07
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2327
0
2327
753893
29
1671
324.0
35.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.845 ± 0.052
0.895 ± 0.015
5.772 ± 0.041
6.05 ± 0.048
3.745 ± 0.035
6.646 ± 0.051
2.185 ± 0.027
6.461 ± 0.041
5.61 ± 0.039
10.11 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.664 ± 0.025
4.48 ± 0.035
4.17 ± 0.03
4.808 ± 0.049
4.287 ± 0.034
6.602 ± 0.039
5.628 ± 0.032
6.746 ± 0.041
1.189 ± 0.02
3.11 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here