Amino acid dipepetide frequency for Psychrobacter piechaudii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.658AlaAla: 7.658 ± 0.15
0.939AlaCys: 0.939 ± 0.037
5.08AlaAsp: 5.08 ± 0.092
5.428AlaGlu: 5.428 ± 0.109
3.044AlaPhe: 3.044 ± 0.076
6.159AlaGly: 6.159 ± 0.108
1.833AlaHis: 1.833 ± 0.057
5.989AlaIle: 5.989 ± 0.098
5.493AlaLys: 5.493 ± 0.089
9.409AlaLeu: 9.409 ± 0.126
2.774AlaMet: 2.774 ± 0.072
3.674AlaAsn: 3.674 ± 0.073
3.107AlaPro: 3.107 ± 0.076
4.121AlaGln: 4.121 ± 0.092
3.515AlaArg: 3.515 ± 0.077
5.456AlaSer: 5.456 ± 0.093
5.136AlaThr: 5.136 ± 0.104
6.076AlaVal: 6.076 ± 0.1
0.967AlaTrp: 0.967 ± 0.042
2.589AlaTyr: 2.589 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.736CysAla: 0.736 ± 0.033
0.119CysCys: 0.119 ± 0.013
0.562CysAsp: 0.562 ± 0.031
0.487CysGlu: 0.487 ± 0.027
0.346CysPhe: 0.346 ± 0.021
0.744CysGly: 0.744 ± 0.036
0.332CysHis: 0.332 ± 0.024
0.554CysIle: 0.554 ± 0.026
0.352CysLys: 0.352 ± 0.024
0.84CysLeu: 0.84 ± 0.036
0.188CysMet: 0.188 ± 0.016
0.337CysAsn: 0.337 ± 0.023
0.456CysPro: 0.456 ± 0.028
0.533CysGln: 0.533 ± 0.029
0.407CysArg: 0.407 ± 0.025
0.554CysSer: 0.554 ± 0.028
0.399CysThr: 0.399 ± 0.021
0.581CysVal: 0.581 ± 0.026
0.096CysTrp: 0.096 ± 0.012
0.321CysTyr: 0.321 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.593AspAla: 4.593 ± 0.078
0.464AspCys: 0.464 ± 0.026
3.229AspAsp: 3.229 ± 0.091
4.136AspGlu: 4.136 ± 0.094
2.352AspPhe: 2.352 ± 0.056
3.202AspGly: 3.202 ± 0.076
1.073AspHis: 1.073 ± 0.039
4.148AspIle: 4.148 ± 0.085
4.002AspLys: 4.002 ± 0.077
5.318AspLeu: 5.318 ± 0.08
1.604AspMet: 1.604 ± 0.053
3.006AspAsn: 3.006 ± 0.067
2.271AspPro: 2.271 ± 0.056
1.949AspGln: 1.949 ± 0.056
2.278AspArg: 2.278 ± 0.049
4.067AspSer: 4.067 ± 0.087
3.144AspThr: 3.144 ± 0.062
3.67AspVal: 3.67 ± 0.07
0.897AspTrp: 0.897 ± 0.039
2.319AspTyr: 2.319 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
5.958GluAla: 5.958 ± 0.105
0.418GluCys: 0.418 ± 0.024
3.519GluAsp: 3.519 ± 0.081
3.739GluGlu: 3.739 ± 0.084
2.146GluPhe: 2.146 ± 0.051
3.938GluGly: 3.938 ± 0.076
1.536GluHis: 1.536 ± 0.048
3.539GluIle: 3.539 ± 0.069
3.209GluLys: 3.209 ± 0.072
6.245GluLeu: 6.245 ± 0.113
1.494GluMet: 1.494 ± 0.044
2.434GluAsn: 2.434 ± 0.06
2.068GluPro: 2.068 ± 0.053
4.059GluGln: 4.059 ± 0.088
2.953GluArg: 2.953 ± 0.066
3.389GluSer: 3.389 ± 0.074
3.101GluThr: 3.101 ± 0.067
4.347GluVal: 4.347 ± 0.077
0.712GluTrp: 0.712 ± 0.034
1.697GluTyr: 1.697 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.392PheAla: 3.392 ± 0.071
0.423PheCys: 0.423 ± 0.025
2.792PheAsp: 2.792 ± 0.064
2.475PheGlu: 2.475 ± 0.058
1.56PhePhe: 1.56 ± 0.058
2.998PheGly: 2.998 ± 0.08
0.673PheHis: 0.673 ± 0.033
2.648PheIle: 2.648 ± 0.073
1.991PheLys: 1.991 ± 0.053
3.023PheLeu: 3.023 ± 0.071
1.056PheMet: 1.056 ± 0.036
1.943PheAsn: 1.943 ± 0.051
1.186PhePro: 1.186 ± 0.042
0.958PheGln: 0.958 ± 0.031
1.186PheArg: 1.186 ± 0.036
2.732PheSer: 2.732 ± 0.054
2.052PheThr: 2.052 ± 0.046
2.52PheVal: 2.52 ± 0.064
0.511PheTrp: 0.511 ± 0.027
1.329PheTyr: 1.329 ± 0.048
0.0PheXaa: 0.0 ± 0.0
Gly
5.116GlyAla: 5.116 ± 0.093
0.801GlyCys: 0.801 ± 0.037
3.4GlyAsp: 3.4 ± 0.071
4.074GlyGlu: 4.074 ± 0.083
2.966GlyPhe: 2.966 ± 0.075
4.698GlyGly: 4.698 ± 0.105
1.431GlyHis: 1.431 ± 0.048
4.999GlyIle: 4.999 ± 0.1
3.959GlyLys: 3.959 ± 0.076
7.066GlyLeu: 7.066 ± 0.103
2.203GlyMet: 2.203 ± 0.058
2.632GlyAsn: 2.632 ± 0.076
1.316GlyPro: 1.316 ± 0.046
2.674GlyGln: 2.674 ± 0.065
2.959GlyArg: 2.959 ± 0.067
4.28GlySer: 4.28 ± 0.087
3.303GlyThr: 3.303 ± 0.063
5.07GlyVal: 5.07 ± 0.089
0.978GlyTrp: 0.978 ± 0.039
2.536GlyTyr: 2.536 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.665HisAla: 1.665 ± 0.051
0.247HisCys: 0.247 ± 0.016
1.171HisAsp: 1.171 ± 0.037
1.146HisGlu: 1.146 ± 0.042
0.86HisPhe: 0.86 ± 0.037
1.468HisGly: 1.468 ± 0.054
0.649HisHis: 0.649 ± 0.037
1.422HisIle: 1.422 ± 0.046
1.112HisLys: 1.112 ± 0.039
2.205HisLeu: 2.205 ± 0.055
0.442HisMet: 0.442 ± 0.024
1.029HisAsn: 1.029 ± 0.041
1.345HisPro: 1.345 ± 0.047
1.085HisGln: 1.085 ± 0.043
1.017HisArg: 1.017 ± 0.037
1.503HisSer: 1.503 ± 0.046
1.146HisThr: 1.146 ± 0.039
1.173HisVal: 1.173 ± 0.038
0.338HisTrp: 0.338 ± 0.021
0.825HisTyr: 0.825 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.179IleAla: 6.179 ± 0.111
0.638IleCys: 0.638 ± 0.031
4.539IleAsp: 4.539 ± 0.091
4.876IleGlu: 4.876 ± 0.083
2.255IlePhe: 2.255 ± 0.052
4.648IleGly: 4.648 ± 0.096
1.34IleHis: 1.34 ± 0.039
4.202IleIle: 4.202 ± 0.092
3.723IleLys: 3.723 ± 0.079
5.217IleLeu: 5.217 ± 0.101
1.565IleMet: 1.565 ± 0.048
3.408IleAsn: 3.408 ± 0.061
2.589IlePro: 2.589 ± 0.059
2.077IleGln: 2.077 ± 0.055
2.664IleArg: 2.664 ± 0.065
4.312IleSer: 4.312 ± 0.076
3.86IleThr: 3.86 ± 0.069
4.044IleVal: 4.044 ± 0.073
0.641IleTrp: 0.641 ± 0.034
1.831IleTyr: 1.831 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
5.224LysAla: 5.224 ± 0.077
0.3LysCys: 0.3 ± 0.02
3.246LysAsp: 3.246 ± 0.071
3.148LysGlu: 3.148 ± 0.07
1.617LysPhe: 1.617 ± 0.044
3.432LysGly: 3.432 ± 0.073
1.145LysHis: 1.145 ± 0.041
3.067LysIle: 3.067 ± 0.068
3.363LysLys: 3.363 ± 0.085
5.777LysLeu: 5.777 ± 0.1
1.414LysMet: 1.414 ± 0.044
2.502LysAsn: 2.502 ± 0.056
2.78LysPro: 2.78 ± 0.079
3.482LysGln: 3.482 ± 0.076
2.855LysArg: 2.855 ± 0.063
3.571LysSer: 3.571 ± 0.076
3.267LysThr: 3.267 ± 0.073
3.825LysVal: 3.825 ± 0.084
0.602LysTrp: 0.602 ± 0.031
1.486LysTyr: 1.486 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
9.917LeuAla: 9.917 ± 0.116
0.891LeuCys: 0.891 ± 0.037
5.61LeuAsp: 5.61 ± 0.09
5.271LeuGlu: 5.271 ± 0.106
3.768LeuPhe: 3.768 ± 0.082
6.758LeuGly: 6.758 ± 0.106
1.922LeuHis: 1.922 ± 0.063
6.526LeuIle: 6.526 ± 0.111
5.572LeuLys: 5.572 ± 0.099
10.105LeuLeu: 10.105 ± 0.164
2.804LeuMet: 2.804 ± 0.071
4.446LeuAsn: 4.446 ± 0.067
5.23LeuPro: 5.23 ± 0.091
4.383LeuGln: 4.383 ± 0.083
3.949LeuArg: 3.949 ± 0.076
7.347LeuSer: 7.347 ± 0.102
6.35LeuThr: 6.35 ± 0.089
6.494LeuVal: 6.494 ± 0.107
1.133LeuTrp: 1.133 ± 0.041
2.621LeuTyr: 2.621 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
2.687MetAla: 2.687 ± 0.069
0.183MetCys: 0.183 ± 0.015
1.175MetAsp: 1.175 ± 0.041
0.971MetGlu: 0.971 ± 0.039
0.801MetPhe: 0.801 ± 0.035
2.055MetGly: 2.055 ± 0.057
0.501MetHis: 0.501 ± 0.027
1.602MetIle: 1.602 ± 0.04
1.352MetLys: 1.352 ± 0.044
2.58MetLeu: 2.58 ± 0.07
0.824MetMet: 0.824 ± 0.038
1.082MetAsn: 1.082 ± 0.039
1.39MetPro: 1.39 ± 0.04
1.437MetGln: 1.437 ± 0.042
1.24MetArg: 1.24 ± 0.039
2.198MetSer: 2.198 ± 0.054
1.935MetThr: 1.935 ± 0.054
1.804MetVal: 1.804 ± 0.054
0.231MetTrp: 0.231 ± 0.019
0.589MetTyr: 0.589 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.672AsnAla: 3.672 ± 0.076
0.397AsnCys: 0.397 ± 0.025
2.446AsnAsp: 2.446 ± 0.06
2.759AsnGlu: 2.759 ± 0.064
1.612AsnPhe: 1.612 ± 0.051
2.527AsnGly: 2.527 ± 0.073
0.97AsnHis: 0.97 ± 0.035
2.967AsnIle: 2.967 ± 0.069
2.898AsnLys: 2.898 ± 0.068
4.201AsnLeu: 4.201 ± 0.072
0.986AsnMet: 0.986 ± 0.036
2.36AsnAsn: 2.36 ± 0.071
2.512AsnPro: 2.512 ± 0.058
2.272AsnGln: 2.272 ± 0.058
2.061AsnArg: 2.061 ± 0.054
3.059AsnSer: 3.059 ± 0.071
2.561AsnThr: 2.561 ± 0.065
2.563AsnVal: 2.563 ± 0.061
0.601AsnTrp: 0.601 ± 0.029
1.376AsnTyr: 1.376 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
3.32ProAla: 3.32 ± 0.084
0.255ProCys: 0.255 ± 0.019
2.609ProAsp: 2.609 ± 0.064
3.393ProGlu: 3.393 ± 0.066
1.657ProPhe: 1.657 ± 0.048
1.799ProGly: 1.799 ± 0.058
0.844ProHis: 0.844 ± 0.033
2.743ProIle: 2.743 ± 0.055
2.686ProLys: 2.686 ± 0.06
4.136ProLeu: 4.136 ± 0.074
1.126ProMet: 1.126 ± 0.042
2.268ProAsn: 2.268 ± 0.06
1.332ProPro: 1.332 ± 0.046
1.8ProGln: 1.8 ± 0.049
1.425ProArg: 1.425 ± 0.05
2.864ProSer: 2.864 ± 0.057
2.52ProThr: 2.52 ± 0.053
3.072ProVal: 3.072 ± 0.062
0.467ProTrp: 0.467 ± 0.027
1.381ProTyr: 1.381 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
5.018GlnAla: 5.018 ± 0.09
0.34GlnCys: 0.34 ± 0.021
2.243GlnAsp: 2.243 ± 0.054
2.084GlnGlu: 2.084 ± 0.055
1.67GlnPhe: 1.67 ± 0.051
2.945GlnGly: 2.945 ± 0.071
1.171GlnHis: 1.171 ± 0.039
2.67GlnIle: 2.67 ± 0.059
2.446GlnLys: 2.446 ± 0.064
5.44GlnLeu: 5.44 ± 0.094
1.191GlnMet: 1.191 ± 0.042
1.88GlnAsn: 1.88 ± 0.048
2.152GlnPro: 2.152 ± 0.067
3.587GlnGln: 3.587 ± 0.101
2.211GlnArg: 2.211 ± 0.059
3.068GlnSer: 3.068 ± 0.069
2.568GlnThr: 2.568 ± 0.057
3.425GlnVal: 3.425 ± 0.083
0.592GlnTrp: 0.592 ± 0.03
1.38GlnTyr: 1.38 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
3.182ArgAla: 3.182 ± 0.064
0.371ArgCys: 0.371 ± 0.021
2.354ArgAsp: 2.354 ± 0.064
2.658ArgGlu: 2.658 ± 0.069
1.913ArgPhe: 1.913 ± 0.049
2.577ArgGly: 2.577 ± 0.068
1.191ArgHis: 1.191 ± 0.041
2.97ArgIle: 2.97 ± 0.062
2.075ArgLys: 2.075 ± 0.058
4.761ArgLeu: 4.761 ± 0.089
1.15ArgMet: 1.15 ± 0.04
1.655ArgAsn: 1.655 ± 0.054
1.792ArgPro: 1.792 ± 0.052
2.374ArgGln: 2.374 ± 0.059
2.291ArgArg: 2.291 ± 0.057
2.51ArgSer: 2.51 ± 0.061
1.929ArgThr: 1.929 ± 0.046
2.772ArgVal: 2.772 ± 0.066
0.574ArgTrp: 0.574 ± 0.027
1.766ArgTyr: 1.766 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
5.123SerAla: 5.123 ± 0.1
0.521SerCys: 0.521 ± 0.028
3.954SerAsp: 3.954 ± 0.081
4.067SerGlu: 4.067 ± 0.071
2.577SerPhe: 2.577 ± 0.054
4.674SerGly: 4.674 ± 0.089
1.498SerHis: 1.498 ± 0.049
4.112SerIle: 4.112 ± 0.078
3.567SerLys: 3.567 ± 0.075
6.978SerLeu: 6.978 ± 0.101
1.665SerMet: 1.665 ± 0.047
3.268SerAsn: 3.268 ± 0.071
2.555SerPro: 2.555 ± 0.059
3.217SerGln: 3.217 ± 0.07
2.876SerArg: 2.876 ± 0.067
4.664SerSer: 4.664 ± 0.108
3.584SerThr: 3.584 ± 0.07
4.344SerVal: 4.344 ± 0.077
0.688SerTrp: 0.688 ± 0.029
2.088SerTyr: 2.088 ± 0.065
0.0SerXaa: 0.0 ± 0.0
Thr
5.326ThrAla: 5.326 ± 0.086
0.454ThrCys: 0.454 ± 0.027
3.25ThrAsp: 3.25 ± 0.071
3.381ThrGlu: 3.381 ± 0.078
1.857ThrPhe: 1.857 ± 0.046
4.121ThrGly: 4.121 ± 0.084
1.365ThrHis: 1.365 ± 0.041
3.29ThrIle: 3.29 ± 0.077
2.775ThrLys: 2.775 ± 0.058
6.103ThrLeu: 6.103 ± 0.075
1.11ThrMet: 1.11 ± 0.037
2.252ThrAsn: 2.252 ± 0.066
3.068ThrPro: 3.068 ± 0.064
2.892ThrGln: 2.892 ± 0.059
2.191ThrArg: 2.191 ± 0.064
3.398ThrSer: 3.398 ± 0.062
3.402ThrThr: 3.402 ± 0.071
3.889ThrVal: 3.889 ± 0.061
0.582ThrTrp: 0.582 ± 0.031
1.573ThrTyr: 1.573 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
6.34ValAla: 6.34 ± 0.099
0.68ValCys: 0.68 ± 0.032
4.056ValAsp: 4.056 ± 0.077
4.068ValGlu: 4.068 ± 0.078
2.561ValPhe: 2.561 ± 0.065
4.739ValGly: 4.739 ± 0.086
1.207ValHis: 1.207 ± 0.04
4.751ValIle: 4.751 ± 0.081
3.544ValLys: 3.544 ± 0.08
6.615ValLeu: 6.615 ± 0.112
2.083ValMet: 2.083 ± 0.054
2.973ValAsn: 2.973 ± 0.068
2.69ValPro: 2.69 ± 0.06
2.323ValGln: 2.323 ± 0.056
2.606ValArg: 2.606 ± 0.06
4.558ValSer: 4.558 ± 0.076
4.072ValThr: 4.072 ± 0.079
5.026ValVal: 5.026 ± 0.102
0.712ValTrp: 0.712 ± 0.035
1.856ValTyr: 1.856 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.845TrpAla: 0.845 ± 0.041
0.135TrpCys: 0.135 ± 0.012
0.503TrpAsp: 0.503 ± 0.027
0.556TrpGlu: 0.556 ± 0.029
0.58TrpPhe: 0.58 ± 0.028
0.756TrpGly: 0.756 ± 0.035
0.345TrpHis: 0.345 ± 0.018
0.748TrpIle: 0.748 ± 0.031
0.42TrpLys: 0.42 ± 0.023
1.809TrpLeu: 1.809 ± 0.058
0.318TrpMet: 0.318 ± 0.021
0.377TrpAsn: 0.377 ± 0.023
0.267TrpPro: 0.267 ± 0.019
1.019TrpGln: 1.019 ± 0.041
0.622TrpArg: 0.622 ± 0.028
0.659TrpSer: 0.659 ± 0.029
0.492TrpThr: 0.492 ± 0.029
0.846TrpVal: 0.846 ± 0.035
0.163TrpTrp: 0.163 ± 0.014
0.427TrpTyr: 0.427 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.498TyrAla: 2.498 ± 0.066
0.369TyrCys: 0.369 ± 0.021
1.98TyrAsp: 1.98 ± 0.053
1.784TyrGlu: 1.784 ± 0.05
1.305TyrPhe: 1.305 ± 0.042
2.223TyrGly: 2.223 ± 0.059
0.822TyrHis: 0.822 ± 0.039
1.659TyrIle: 1.659 ± 0.048
1.561TyrLys: 1.561 ± 0.045
3.272TyrLeu: 3.272 ± 0.07
0.641TyrMet: 0.641 ± 0.03
1.272TyrAsn: 1.272 ± 0.036
1.59TyrPro: 1.59 ± 0.045
1.838TyrGln: 1.838 ± 0.048
1.56TyrArg: 1.56 ± 0.047
1.831TyrSer: 1.831 ± 0.052
1.458TyrThr: 1.458 ± 0.05
1.919TyrVal: 1.919 ± 0.051
0.403TyrTrp: 0.403 ± 0.024
1.116TyrTyr: 1.116 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2327 proteins (753893 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski