Salibacter halophilus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Salibacteraceae; Salibacter

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2675 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N6M6E2|A0A6N6M6E2_9FLAO Short-chain fatty acid transporter OS=Salibacter halophilus OX=1803916 GN=F3059_09645 PE=4 SV=1
II1 pKa = 7.43TDD3 pKa = 3.94GSNNVSTSTAIVTVEE18 pKa = 3.89DD19 pKa = 4.52TIAPNIQTQSVTLYY33 pKa = 10.78LDD35 pKa = 3.55SLGQASTTAMSIDD48 pKa = 3.77AGSSDD53 pKa = 3.54NCAIDD58 pKa = 3.88TMFLSEE64 pKa = 4.46NNFDD68 pKa = 4.14CSHH71 pKa = 6.98IGTNTVQLIATDD83 pKa = 3.79VNGNTDD89 pKa = 2.78SSSAVVTVEE98 pKa = 3.7DD99 pKa = 4.56TIAPEE104 pKa = 4.44VITQGVTLYY113 pKa = 10.99LDD115 pKa = 3.61STGTAVLTPSVFDD128 pKa = 3.92NGSNDD133 pKa = 3.17NCSIDD138 pKa = 4.63SMSLSQDD145 pKa = 2.84TFTCSDD151 pKa = 3.63LGLHH155 pKa = 6.0VVDD158 pKa = 5.57LHH160 pKa = 6.33VTDD163 pKa = 3.97QSGNGATSNALVSVTDD179 pKa = 4.42TIAPLLRR186 pKa = 11.84SFSNITVYY194 pKa = 10.96LDD196 pKa = 3.36SSGTASIDD204 pKa = 3.92AGMLDD209 pKa = 4.03SNSVDD214 pKa = 3.14NCSLDD219 pKa = 3.55TIYY222 pKa = 11.09LSRR225 pKa = 11.84TTLGCNDD232 pKa = 4.1LGSTTVEE239 pKa = 4.56LIGKK243 pKa = 8.6DD244 pKa = 3.06GSMNEE249 pKa = 3.72SRR251 pKa = 11.84ILVNLTVEE259 pKa = 4.39DD260 pKa = 3.98TLAPTIEE267 pKa = 4.74CPSSFDD273 pKa = 3.13ACAGIVNFPDD283 pKa = 3.66AVSNDD288 pKa = 3.26NCTSTVTNTGNVFSGDD304 pKa = 3.44SLTEE308 pKa = 3.97GEE310 pKa = 4.59YY311 pKa = 9.21VTSFEE316 pKa = 4.83AVDD319 pKa = 3.91GSGNVASCTTSFSVNAIPEE338 pKa = 4.04VDD340 pKa = 4.24LGADD344 pKa = 3.43TAVGPGHH351 pKa = 5.93VVSFSVNDD359 pKa = 3.88TVSEE363 pKa = 4.32YY364 pKa = 10.96LWSTGDD370 pKa = 3.37TTRR373 pKa = 11.84SLDD376 pKa = 3.92LKK378 pKa = 10.87VLTDD382 pKa = 3.31TTVWISVTDD391 pKa = 3.93SNSCTGSDD399 pKa = 3.31TATVTTLTSIKK410 pKa = 10.03RR411 pKa = 11.84SKK413 pKa = 9.57KK414 pKa = 7.21TVNVSVYY421 pKa = 9.71PNPTRR426 pKa = 11.84GEE428 pKa = 4.19VKK430 pKa = 10.5VEE432 pKa = 3.7LLGVEE437 pKa = 4.12NLNYY441 pKa = 10.43HH442 pKa = 7.2LIDD445 pKa = 3.31MAGRR449 pKa = 11.84ILQSGNMQNGLNRR462 pKa = 11.84IDD464 pKa = 3.85LSRR467 pKa = 11.84YY468 pKa = 6.07ATSVYY473 pKa = 10.47YY474 pKa = 10.63LQLQNEE480 pKa = 4.3QGEE483 pKa = 4.96GIKK486 pKa = 10.7VMRR489 pKa = 11.84LVKK492 pKa = 10.34QQ493 pKa = 3.31

Molecular weight: 51.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N6M4S9|A0A6N6M4S9_9FLAO Uncharacterized protein OS=Salibacter halophilus OX=1803916 GN=F3059_07180 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 9.07RR12 pKa = 11.84KK13 pKa = 8.26NKK15 pKa = 9.73HH16 pKa = 4.02GFRR19 pKa = 11.84EE20 pKa = 4.14RR21 pKa = 11.84MSSKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 8.97VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.11GRR39 pKa = 11.84KK40 pKa = 8.0KK41 pKa = 10.56VSVSSEE47 pKa = 3.8PRR49 pKa = 11.84HH50 pKa = 5.89KK51 pKa = 10.65RR52 pKa = 3.21

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2675

0

2675

957364

38

3560

357.9

40.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.06 ± 0.046

0.778 ± 0.024

6.1 ± 0.035

6.993 ± 0.069

5.032 ± 0.042

6.704 ± 0.047

1.81 ± 0.018

7.141 ± 0.045

7.069 ± 0.078

8.791 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.023

5.902 ± 0.053

3.571 ± 0.025

3.604 ± 0.027

3.871 ± 0.047

7.146 ± 0.062

5.609 ± 0.067

6.433 ± 0.035

1.11 ± 0.015

4.073 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski