Alteromonas phage vB_AmeM_PT11-V22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Myoalterovirus; Alteromonas virus PT11-V22

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 156 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C0R1N9|A0A6C0R1N9_9CAUD Thymidylate synthase OS=Alteromonas phage vB_AmeM_PT11-V22 OX=2704031 PE=4 SV=1
MM1 pKa = 7.24GVDD4 pKa = 3.46RR5 pKa = 11.84VAILFRR11 pKa = 11.84DD12 pKa = 3.85TFTEE16 pKa = 4.08SSNTNLIDD24 pKa = 3.47HH25 pKa = 6.6TPEE28 pKa = 3.69QGGPWVYY35 pKa = 10.55EE36 pKa = 3.66AGVPDD41 pKa = 3.36NAAIVRR47 pKa = 11.84SEE49 pKa = 4.62DD50 pKa = 3.49EE51 pKa = 3.92LRR53 pKa = 11.84LNGSIVDD60 pKa = 3.76GFYY63 pKa = 10.95VYY65 pKa = 10.85DD66 pKa = 3.64GTANAGGYY74 pKa = 9.41IATTPASSLVTPEE87 pKa = 4.35KK88 pKa = 9.57YY89 pKa = 10.31CCIRR93 pKa = 11.84LVDD96 pKa = 3.92SNNFFGIEE104 pKa = 3.83FRR106 pKa = 11.84GTGSSGVGFVKK117 pKa = 10.55SVGGVITDD125 pKa = 4.84LYY127 pKa = 11.18SEE129 pKa = 4.42TPEE132 pKa = 4.75AGAVYY137 pKa = 10.78YY138 pKa = 10.96LDD140 pKa = 5.61DD141 pKa = 4.59NGNSLVFKK149 pKa = 9.89KK150 pKa = 10.93DD151 pKa = 3.11NGATTTLYY159 pKa = 10.46TEE161 pKa = 4.34NDD163 pKa = 3.71SEE165 pKa = 5.35FSTEE169 pKa = 3.89TKK171 pKa = 10.27KK172 pKa = 11.15GFVSLGTGNSNPSTYY187 pKa = 10.32YY188 pKa = 10.59EE189 pKa = 4.35SGTLEE194 pKa = 4.36APTGVTINSLSDD206 pKa = 3.31YY207 pKa = 10.06SAKK210 pKa = 10.35PRR212 pKa = 11.84DD213 pKa = 3.89ANGDD217 pKa = 3.33ATFTVSGGCPDD228 pKa = 3.3GTSAIEE234 pKa = 3.84YY235 pKa = 10.39SFDD238 pKa = 3.81EE239 pKa = 4.56ITWLTLDD246 pKa = 4.45ASPSGGAYY254 pKa = 10.32SGTVVVNGQTDD265 pKa = 3.4IFVRR269 pKa = 11.84SADD272 pKa = 3.96DD273 pKa = 3.55NALTASVVGITSAMPIVLWGQSNCDD298 pKa = 2.77GRR300 pKa = 11.84GINNQAVASGSIVPLMYY317 pKa = 10.53KK318 pKa = 10.19SGAVTQMADD327 pKa = 3.32PTGITVDD334 pKa = 3.49SAGSLWPLVVKK345 pKa = 8.99YY346 pKa = 10.14FIDD349 pKa = 3.34QGIPVVMANVAQGGKK364 pKa = 7.43TVAEE368 pKa = 4.49FEE370 pKa = 4.77PSTSLYY376 pKa = 11.04SRR378 pKa = 11.84ISDD381 pKa = 4.01FAANFGSPEE390 pKa = 3.96LAISVIGEE398 pKa = 4.02ADD400 pKa = 3.17SGGTTKK406 pKa = 11.06ADD408 pKa = 3.58FKK410 pKa = 10.93TRR412 pKa = 11.84YY413 pKa = 9.96LNIAQTMNTDD423 pKa = 3.3YY424 pKa = 9.54GTEE427 pKa = 4.03TYY429 pKa = 10.35AVKK432 pKa = 10.57FPVGTATNTTTQTEE446 pKa = 3.66IRR448 pKa = 11.84EE449 pKa = 4.47AYY451 pKa = 10.25DD452 pKa = 3.37EE453 pKa = 5.83LIDD456 pKa = 3.68EE457 pKa = 4.3NAFIKK462 pKa = 10.75FGGDD466 pKa = 3.24LSVIDD471 pKa = 3.77IDD473 pKa = 4.65SGIGHH478 pKa = 7.69DD479 pKa = 4.03SLHH482 pKa = 6.67LKK484 pKa = 8.87TDD486 pKa = 3.19SHH488 pKa = 8.03LSEE491 pKa = 5.72GDD493 pKa = 3.58VIIRR497 pKa = 11.84SALAGIVPDD506 pKa = 3.44VTKK509 pKa = 10.45PVITLLGTPSVVITNGSNYY528 pKa = 9.09TDD530 pKa = 3.72AGATASDD537 pKa = 4.74NIDD540 pKa = 3.17GDD542 pKa = 3.63ITSNIVTVNPVNTNVSATYY561 pKa = 8.89TVTYY565 pKa = 10.59NVTDD569 pKa = 3.73SAGNAADD576 pKa = 3.6EE577 pKa = 4.59VTRR580 pKa = 11.84TVTVQDD586 pKa = 3.83AASDD590 pKa = 3.95NQPPTSNAGADD601 pKa = 3.51QTNIAAGATVTLNGTGSTDD620 pKa = 3.07SDD622 pKa = 3.87GTIVSYY628 pKa = 11.05AWTQTAGDD636 pKa = 3.91IVTLISATSASPTFTAPTTANAQTLTFEE664 pKa = 4.7LTVTDD669 pKa = 5.03DD670 pKa = 4.02GGLTSAPDD678 pKa = 3.58SVDD681 pKa = 3.32VQVNALTLPSVSISAVSQVNTGQSFSPTVTVSGYY715 pKa = 11.33DD716 pKa = 3.41NLLWEE721 pKa = 4.8CTSGQSPSFSSTTAEE736 pKa = 4.27APFITLNEE744 pKa = 4.47GGVHH748 pKa = 5.58TFRR751 pKa = 11.84LTATNISGSVSDD763 pKa = 4.02TFNITGMVQVVEE775 pKa = 4.41SALNLSWEE783 pKa = 4.17GLPDD787 pKa = 3.32GTYY790 pKa = 9.56TVLFIDD796 pKa = 4.37RR797 pKa = 11.84DD798 pKa = 3.69NDD800 pKa = 3.97SVLSRR805 pKa = 11.84SVTSSNEE812 pKa = 3.46AAAVTLPLPVGTKK825 pKa = 9.7VDD827 pKa = 3.97YY828 pKa = 10.39YY829 pKa = 10.3WYY831 pKa = 10.83NEE833 pKa = 4.07TSGDD837 pKa = 3.59AVLNTGATEE846 pKa = 3.85

Molecular weight: 88.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C0R328|A0A6C0R328_9CAUD Uncharacterized protein OS=Alteromonas phage vB_AmeM_PT11-V22 OX=2704031 PE=4 SV=1
MM1 pKa = 7.38HH2 pKa = 7.35FLGRR6 pKa = 11.84SLTTKK11 pKa = 9.6PCSINLRR18 pKa = 11.84VNVLMLPHH26 pKa = 5.99FTISTGSVRR35 pKa = 11.84SVKK38 pKa = 10.09PINLSRR44 pKa = 11.84VLIKK48 pKa = 10.7LEE50 pKa = 3.95YY51 pKa = 8.29VQPFISDD58 pKa = 3.56LTVIAQLVNRR68 pKa = 11.84IVTIFSPLFHH78 pKa = 7.08CGIIANN84 pKa = 4.16

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

156

0

156

27604

29

1430

176.9

20.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.785 ± 0.302

1.33 ± 0.117

6.724 ± 0.163

8.22 ± 0.256

4.148 ± 0.146

6.564 ± 0.167

1.895 ± 0.135

6.245 ± 0.263

7.477 ± 0.392

8.169 ± 0.201

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.149

5.456 ± 0.25

2.945 ± 0.12

3.329 ± 0.108

3.927 ± 0.148

6.51 ± 0.211

6.361 ± 0.386

6.93 ± 0.205

1.337 ± 0.089

4.155 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski