Alfalfa mosaic virus (strain 425 / isolate Leiden)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Alfamovirus; Alfalfa mosaic virus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03595|MVP_AMVLE Movement protein OS=Alfalfa mosaic virus (strain 425 / isolate Leiden) OX=12322 GN=ORF3a PE=3 SV=1
MM1 pKa = 6.9FTLLRR6 pKa = 11.84CLGFGVNEE14 pKa = 4.09PTNTSSSEE22 pKa = 4.0YY23 pKa = 8.51VPEE26 pKa = 4.0YY27 pKa = 10.47SVEE30 pKa = 4.17EE31 pKa = 4.0ISNEE35 pKa = 3.91VAEE38 pKa = 4.93LDD40 pKa = 3.83SVDD43 pKa = 5.75PLFQCYY49 pKa = 7.62KK50 pKa = 9.98HH51 pKa = 6.49VFVSLMLVRR60 pKa = 11.84KK61 pKa = 6.15MTQAAEE67 pKa = 4.17DD68 pKa = 3.9FLEE71 pKa = 4.53SFGGEE76 pKa = 3.89FDD78 pKa = 4.27SPCCRR83 pKa = 11.84VYY85 pKa = 10.43RR86 pKa = 11.84LYY88 pKa = 11.1RR89 pKa = 11.84HH90 pKa = 6.23FVNEE94 pKa = 4.03DD95 pKa = 3.56DD96 pKa = 4.17APAWAIPNVVNEE108 pKa = 4.64DD109 pKa = 3.73SYY111 pKa = 12.28DD112 pKa = 3.51DD113 pKa = 3.7YY114 pKa = 11.89AYY116 pKa = 10.93LRR118 pKa = 11.84EE119 pKa = 4.25EE120 pKa = 4.61LDD122 pKa = 4.73AIDD125 pKa = 5.69SSFEE129 pKa = 3.86LLNEE133 pKa = 3.76EE134 pKa = 4.79RR135 pKa = 11.84EE136 pKa = 4.12LSEE139 pKa = 3.79ITDD142 pKa = 3.58RR143 pKa = 11.84LNALRR148 pKa = 11.84FFPVSKK154 pKa = 9.99TEE156 pKa = 4.07ALPVANVQEE165 pKa = 4.27VKK167 pKa = 10.72LISEE171 pKa = 5.21TYY173 pKa = 8.54QLLMTFINYY182 pKa = 9.6SDD184 pKa = 3.94EE185 pKa = 4.75NIPSEE190 pKa = 4.35MPAPLLDD197 pKa = 4.06EE198 pKa = 5.84LGMLPEE204 pKa = 4.36EE205 pKa = 4.65LGPLNEE211 pKa = 4.71IEE213 pKa = 4.97DD214 pKa = 4.41IKK216 pKa = 11.04PVAAPITLLSEE227 pKa = 4.36FRR229 pKa = 11.84ASDD232 pKa = 3.31NAKK235 pKa = 9.86PLDD238 pKa = 3.7IVEE241 pKa = 5.39IIPDD245 pKa = 3.67VSPTKK250 pKa = 10.07PYY252 pKa = 10.53EE253 pKa = 3.99AVISGNDD260 pKa = 2.84WMTLGRR266 pKa = 11.84IIPTTPVPTIRR277 pKa = 11.84DD278 pKa = 3.52VFFSGLSRR286 pKa = 11.84HH287 pKa = 6.0GSPEE291 pKa = 4.06VIQNALDD298 pKa = 3.52EE299 pKa = 4.7FLPLHH304 pKa = 6.44HH305 pKa = 7.23SIDD308 pKa = 3.44DD309 pKa = 3.77KK310 pKa = 11.71YY311 pKa = 9.29FQEE314 pKa = 4.24WVEE317 pKa = 4.15TSDD320 pKa = 4.5KK321 pKa = 11.33SLDD324 pKa = 3.54VDD326 pKa = 3.78PCRR329 pKa = 11.84IDD331 pKa = 4.89LSVFNNWQSSEE342 pKa = 3.77NCYY345 pKa = 10.13EE346 pKa = 3.88PRR348 pKa = 11.84FKK350 pKa = 10.39TGALSTRR357 pKa = 11.84KK358 pKa = 8.41GTQTEE363 pKa = 3.84ALLAIKK369 pKa = 9.37KK370 pKa = 10.41RR371 pKa = 11.84NMNVPNLGQIYY382 pKa = 10.06DD383 pKa = 3.92VNSVANSVVNKK394 pKa = 10.41LLTTVIDD401 pKa = 3.97PDD403 pKa = 4.33KK404 pKa = 11.43LCMFPDD410 pKa = 5.76FISEE414 pKa = 4.42GEE416 pKa = 3.85VSYY419 pKa = 10.54FQDD422 pKa = 3.77YY423 pKa = 10.88IVGKK427 pKa = 10.24NPDD430 pKa = 3.78PEE432 pKa = 5.45LYY434 pKa = 10.37SDD436 pKa = 3.93PLGVRR441 pKa = 11.84SIDD444 pKa = 3.73SYY446 pKa = 11.36KK447 pKa = 11.12HH448 pKa = 5.52MIKK451 pKa = 10.34SVLKK455 pKa = 9.54PVEE458 pKa = 4.59DD459 pKa = 3.68NSLHH463 pKa = 6.86LEE465 pKa = 4.08RR466 pKa = 11.84PMPATITYY474 pKa = 8.77HH475 pKa = 7.14DD476 pKa = 4.57KK477 pKa = 11.09DD478 pKa = 3.48IVMSSSPIFLAAAARR493 pKa = 11.84LMLILRR499 pKa = 11.84DD500 pKa = 4.09KK501 pKa = 10.37ITIPSGKK508 pKa = 9.15FHH510 pKa = 6.67QLFSIDD516 pKa = 4.43AEE518 pKa = 4.06AFDD521 pKa = 4.73ASFHH525 pKa = 5.89FKK527 pKa = 10.59EE528 pKa = 3.9IDD530 pKa = 3.31FSKK533 pKa = 10.58FDD535 pKa = 3.28KK536 pKa = 10.89SQNEE540 pKa = 4.02LHH542 pKa = 6.83HH543 pKa = 6.82LIQEE547 pKa = 4.37RR548 pKa = 11.84FLKK551 pKa = 10.83YY552 pKa = 10.44LGIPNEE558 pKa = 4.48FLTLWFNAHH567 pKa = 5.81RR568 pKa = 11.84KK569 pKa = 9.26SRR571 pKa = 11.84ISDD574 pKa = 3.44SKK576 pKa = 11.08NGVFFNVDD584 pKa = 3.72FQRR587 pKa = 11.84RR588 pKa = 11.84TGDD591 pKa = 3.07ALTYY595 pKa = 10.34LGNTIVTLACLCHH608 pKa = 7.66VYY610 pKa = 11.25DD611 pKa = 5.97LMDD614 pKa = 4.9PNVKK618 pKa = 9.9FVVASGDD625 pKa = 3.45DD626 pKa = 3.72SLIGTVEE633 pKa = 3.98EE634 pKa = 4.55LPRR637 pKa = 11.84DD638 pKa = 3.62QEE640 pKa = 4.42FLFTTLFNLEE650 pKa = 3.95AKK652 pKa = 10.2FPHH655 pKa = 5.97NQPFICSKK663 pKa = 10.61FLITMPTTSGGKK675 pKa = 9.38VVLPIPNPLKK685 pKa = 10.72LLIRR689 pKa = 11.84LGSKK693 pKa = 9.63KK694 pKa = 10.5VNADD698 pKa = 4.42IFDD701 pKa = 3.33EE702 pKa = 5.39WYY704 pKa = 10.56QSWIDD709 pKa = 3.71IIGGFNDD716 pKa = 3.21HH717 pKa = 6.83HH718 pKa = 7.25VIRR721 pKa = 11.84CVAAMTAHH729 pKa = 7.23RR730 pKa = 11.84YY731 pKa = 8.54LRR733 pKa = 11.84RR734 pKa = 11.84PSLYY738 pKa = 10.67LEE740 pKa = 4.62AALEE744 pKa = 4.16SLGKK748 pKa = 10.13IFAGKK753 pKa = 7.12TLCKK757 pKa = 10.16EE758 pKa = 3.93CLFNEE763 pKa = 4.07KK764 pKa = 10.31HH765 pKa = 5.65EE766 pKa = 5.19SNVKK770 pKa = 9.5IKK772 pKa = 9.98PRR774 pKa = 11.84RR775 pKa = 11.84VKK777 pKa = 10.42KK778 pKa = 9.65SHH780 pKa = 6.2SDD782 pKa = 2.85ARR784 pKa = 11.84SRR786 pKa = 11.84ARR788 pKa = 11.84RR789 pKa = 11.84AA790 pKa = 3.05

Molecular weight: 89.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03593|RDRP_AMVLE RNA-directed RNA polymerase 2a OS=Alfalfa mosaic virus (strain 425 / isolate Leiden) OX=12322 GN=ORF2a PE=3 SV=2
MM1 pKa = 7.77SSSQKK6 pKa = 10.31KK7 pKa = 10.38AGGKK11 pKa = 9.36AGKK14 pKa = 6.82PTKK17 pKa = 10.18RR18 pKa = 11.84SQNYY22 pKa = 8.54AALRR26 pKa = 11.84KK27 pKa = 9.29AQLPKK32 pKa = 10.5PPALKK37 pKa = 10.71VPVVKK42 pKa = 8.86PTNTILPQTGCVWQSLGTPLSLSSFNGLGARR73 pKa = 11.84FLYY76 pKa = 10.8SFLKK80 pKa = 10.82DD81 pKa = 3.48FVGPRR86 pKa = 11.84ILEE89 pKa = 3.81EE90 pKa = 3.9DD91 pKa = 4.35LIYY94 pKa = 11.15RR95 pKa = 11.84MVFSITPSHH104 pKa = 6.87AGTFCLTDD112 pKa = 3.62DD113 pKa = 3.77VTTEE117 pKa = 3.97DD118 pKa = 3.79GRR120 pKa = 11.84AVAHH124 pKa = 6.19GNPMQEE130 pKa = 4.26FPHH133 pKa = 6.38GAFHH137 pKa = 7.23ANEE140 pKa = 4.02KK141 pKa = 10.73FGFEE145 pKa = 4.39LVFTAPTHH153 pKa = 7.53AGMQNQNFKK162 pKa = 10.65HH163 pKa = 6.06SYY165 pKa = 10.11AVALCLDD172 pKa = 4.68FDD174 pKa = 4.28AQPEE178 pKa = 4.57GSKK181 pKa = 10.81NPSFRR186 pKa = 11.84FNEE189 pKa = 3.79VWVEE193 pKa = 4.28RR194 pKa = 11.84KK195 pKa = 9.65AFPRR199 pKa = 11.84AGPLRR204 pKa = 11.84SLITVGLFDD213 pKa = 5.08EE214 pKa = 5.92ADD216 pKa = 3.68DD217 pKa = 4.83LDD219 pKa = 3.91RR220 pKa = 11.84HH221 pKa = 5.68

Molecular weight: 24.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2437

221

1126

609.3

68.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.427 ± 0.707

1.805 ± 0.325

6.524 ± 0.517

5.745 ± 0.689

4.924 ± 0.671

5.211 ± 0.755

2.831 ± 0.228

5.745 ± 0.674

6.278 ± 0.392

9.561 ± 0.549

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.929 ± 0.242

4.391 ± 0.267

5.252 ± 0.73

2.544 ± 0.312

5.129 ± 0.395

8.453 ± 0.521

5.622 ± 0.374

6.648 ± 0.134

0.78 ± 0.091

3.201 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski