Phytomonas sp. isolate Hart1

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Phytomonas; unclassified Phytomonas

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6442 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6LAI5|W6LAI5_9TRYP Uncharacterized protein OS=Phytomonas sp. isolate Hart1 OX=134013 GN=GSHART1_T00007993001 PE=4 SV=1
MM1 pKa = 7.45ANEE4 pKa = 3.75SGMRR8 pKa = 11.84VDD10 pKa = 4.3MIVIHH15 pKa = 6.48EE16 pKa = 4.32EE17 pKa = 3.9GALPSPQEE25 pKa = 4.07LLCSYY30 pKa = 9.88PYY32 pKa = 11.23NLLSDD37 pKa = 4.52LYY39 pKa = 8.41EE40 pKa = 4.08TRR42 pKa = 11.84EE43 pKa = 3.87ALLFLNLDD51 pKa = 4.15EE52 pKa = 4.89IFSGEE57 pKa = 4.24MDD59 pKa = 4.79SLPLWAYY66 pKa = 10.22EE67 pKa = 4.09EE68 pKa = 4.41VPPFQVSEE76 pKa = 4.29SS77 pKa = 3.58

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6LH49|W6LH49_9TRYP C2 domain-containing protein OS=Phytomonas sp. isolate Hart1 OX=134013 GN=GSHART1_T00002136001 PE=4 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84RR3 pKa = 11.84WPRR6 pKa = 11.84WTGPWRR12 pKa = 11.84GSGGICGGAARR23 pKa = 11.84GGACGSPPTPPPTPPRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84QGPGKK46 pKa = 10.47GKK48 pKa = 8.02TRR50 pKa = 11.84EE51 pKa = 3.97KK52 pKa = 10.53RR53 pKa = 11.84GTSPRR58 pKa = 11.84RR59 pKa = 11.84CMLWIVFSTTRR70 pKa = 11.84PRR72 pKa = 11.84SRR74 pKa = 11.84GSRR77 pKa = 11.84RR78 pKa = 11.84NRR80 pKa = 11.84GSAGTTAAAAAPPRR94 pKa = 11.84SAGRR98 pKa = 11.84PSSPTPSRR106 pKa = 11.84RR107 pKa = 11.84TSWSTSAAFSSPPSRR122 pKa = 11.84RR123 pKa = 11.84PGGRR127 pKa = 11.84RR128 pKa = 11.84GGFFFLFFFFFFLFSFLRR146 pKa = 11.84GLL148 pKa = 3.72

Molecular weight: 16.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6442

0

6442

2938716

20

6650

456.2

50.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.259 ± 0.033

1.887 ± 0.014

5.075 ± 0.015

6.598 ± 0.03

4.0 ± 0.018

5.753 ± 0.027

2.729 ± 0.013

5.0 ± 0.023

5.047 ± 0.027

9.936 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.012

4.228 ± 0.02

5.131 ± 0.027

3.771 ± 0.019

6.392 ± 0.027

8.36 ± 0.034

5.523 ± 0.021

6.272 ± 0.023

1.084 ± 0.009

2.777 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski