Hanseniaspora uvarum (Yeast) (Kloeckera apiculata)
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4059 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E5S1Z9|A0A1E5S1Z9_HANUV Ceramide synthase subunit LIP1 OS=Hanseniaspora uvarum OX=29833 GN=AWRI3580_g76 PE=4 SV=1
MM1 pKa = 7.59 QYY3 pKa = 7.88 TTITSIFLLTISLLTQSTSAIGTLGINVASSGVDD37 pKa = 3.5 GNCRR41 pKa = 11.84 DD42 pKa = 3.53 SDD44 pKa = 3.72 SWSEE48 pKa = 4.07 ALEE51 pKa = 4.17 KK52 pKa = 10.92 ASAFTNYY59 pKa = 8.96 IKK61 pKa = 10.0 TYY63 pKa = 10.62 AVSDD67 pKa = 4.0 CNMLEE72 pKa = 4.27 NIVPALNSNTNMKK85 pKa = 9.47 VWMGVWEE92 pKa = 4.04 VDD94 pKa = 3.35 EE95 pKa = 4.64 AHH97 pKa = 7.04 FDD99 pKa = 3.72 AEE101 pKa = 4.39 KK102 pKa = 10.96 DD103 pKa = 3.38 ALTTYY108 pKa = 10.44 LPSISKK114 pKa = 10.62 DD115 pKa = 3.43 SIAGISVGSEE125 pKa = 3.03 ALYY128 pKa = 10.84 RR129 pKa = 11.84 GDD131 pKa = 4.37 LTPQALASKK140 pKa = 9.91 ISEE143 pKa = 4.13 IKK145 pKa = 10.68 SLLSDD150 pKa = 3.27 ITDD153 pKa = 3.46 KK154 pKa = 11.5 DD155 pKa = 3.85 GNSYY159 pKa = 11.03 SGVSVGTVDD168 pKa = 4.59 SWNIWVNGTNAPAIQAADD186 pKa = 3.9 YY187 pKa = 11.22 IMVNAFPFWQYY198 pKa = 7.92 QTANNQSHH206 pKa = 6.35 SLVDD210 pKa = 4.17 DD211 pKa = 3.7 VMQAVKK217 pKa = 10.51 AIQEE221 pKa = 4.21 AKK223 pKa = 9.59 GTDD226 pKa = 3.3 DD227 pKa = 4.77 FYY229 pKa = 11.13 ISVGEE234 pKa = 4.27 TGAPTGGNEE243 pKa = 4.19 LTSGQAPLTVDD254 pKa = 3.1 NASDD258 pKa = 4.12 LFQEE262 pKa = 5.73 GICTLLAYY270 pKa = 10.29 GIDD273 pKa = 3.66 VNIFEE278 pKa = 5.22 LQDD281 pKa = 3.54 EE282 pKa = 4.58 PTKK285 pKa = 10.67 PLATADD291 pKa = 4.55 DD292 pKa = 4.87 GSSSDD297 pKa = 4.1 VEE299 pKa = 4.44 RR300 pKa = 11.84 HH301 pKa = 4.36 WGVYY305 pKa = 8.53 DD306 pKa = 3.19 TDD308 pKa = 4.08 YY309 pKa = 11.28 NLKK312 pKa = 10.61 YY313 pKa = 10.76 SLTCSYY319 pKa = 11.2 GSNN322 pKa = 3.33
Molecular weight: 34.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.91
IPC_protein 3.923
Toseland 3.706
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.846
Rodwell 3.745
Grimsley 3.617
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.859
Sillero 4.05
Patrickios 0.973
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.91
Protein with the highest isoelectric point:
>tr|A0A1E5RJZ3|A0A1E5RJZ3_HANUV Cell division control protein 53 OS=Hanseniaspora uvarum OX=29833 GN=AWRI3580_g2889 PE=3 SV=1
MM1 pKa = 7.52 DD2 pKa = 5.56 TIRR5 pKa = 11.84 TGITGKK11 pKa = 10.68 YY12 pKa = 7.27 GVRR15 pKa = 11.84 YY16 pKa = 9.34 GSSLRR21 pKa = 11.84 RR22 pKa = 11.84 QVKK25 pKa = 9.69 KK26 pKa = 11.14 LEE28 pKa = 4.21 VQQHH32 pKa = 4.66 SRR34 pKa = 11.84 YY35 pKa = 10.26 DD36 pKa = 3.35 CSFCGKK42 pKa = 10.23 KK43 pKa = 9.77 SVKK46 pKa = 10.2 RR47 pKa = 11.84 GATGIWNCGSCKK59 pKa = 9.43 KK60 pKa = 8.82 TVAGGAYY67 pKa = 8.32 TVSTAAAATVRR78 pKa = 11.84 STIRR82 pKa = 11.84 RR83 pKa = 11.84 LRR85 pKa = 11.84 DD86 pKa = 3.24 MVEE89 pKa = 3.53 AA90 pKa = 4.79
Molecular weight: 9.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.677
IPC_protein 10.087
Toseland 10.496
ProMoST 10.116
Dawson 10.613
Bjellqvist 10.306
Wikipedia 10.789
Rodwell 11.008
Grimsley 10.672
Solomon 10.672
Lehninger 10.643
Nozaki 10.511
DTASelect 10.277
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.54
Patrickios 10.76
IPC_peptide 10.672
IPC2_peptide 9.399
IPC2.peptide.svr19 8.527
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4059
0
4059
2125948
66
4955
523.8
59.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.36 ± 0.036
1.123 ± 0.011
6.269 ± 0.03
6.933 ± 0.035
4.801 ± 0.025
4.332 ± 0.041
1.799 ± 0.012
7.475 ± 0.033
8.957 ± 0.044
9.61 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.126 ± 0.015
7.721 ± 0.048
3.361 ± 0.025
3.674 ± 0.022
3.08 ± 0.021
8.31 ± 0.052
5.523 ± 0.042
5.354 ± 0.023
0.822 ± 0.01
3.864 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here