Aspergillus arachidicola
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12090 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G7EM66|A0A2G7EM66_9EURO Beta-glucosidase (Fragment) OS=Aspergillus arachidicola OX=656916 GN=AARAC_009615 PE=3 SV=1
MM1 pKa = 7.7 ADD3 pKa = 3.51 EE4 pKa = 5.26 AGSIYY9 pKa = 10.71 DD10 pKa = 3.85 EE11 pKa = 4.95 IEE13 pKa = 4.34 IEE15 pKa = 5.79 DD16 pKa = 3.79 MTFDD20 pKa = 6.24 PITQLYY26 pKa = 9.73 HH27 pKa = 5.63 YY28 pKa = 7.55 PCPCGDD34 pKa = 3.67 RR35 pKa = 11.84 FEE37 pKa = 5.7 IMIDD41 pKa = 3.49 DD42 pKa = 4.19 LRR44 pKa = 11.84 DD45 pKa = 3.58 GEE47 pKa = 4.73 EE48 pKa = 3.84 IAVCPSCSLKK58 pKa = 10.28 IRR60 pKa = 11.84 VIFDD64 pKa = 3.02 VDD66 pKa = 4.13 DD67 pKa = 3.69 LHH69 pKa = 9.25 KK70 pKa = 10.81 DD71 pKa = 3.63 DD72 pKa = 4.29 QQQGPSAVAVQAA84 pKa = 4.09
Molecular weight: 9.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A2G7FUM2|A0A2G7FUM2_9EURO RING-type domain-containing protein OS=Aspergillus arachidicola OX=656916 GN=AARAC_008645 PE=4 SV=1
MM1 pKa = 7.95 PNRR4 pKa = 11.84 PQFSGRR10 pKa = 11.84 TMDD13 pKa = 3.38 LTLFSFTRR21 pKa = 11.84 AVDD24 pKa = 4.12 LVACIIWARR33 pKa = 11.84 WRR35 pKa = 11.84 RR36 pKa = 11.84 WRR38 pKa = 11.84 SARR41 pKa = 11.84 GRR43 pKa = 11.84 WSRR46 pKa = 11.84 AEE48 pKa = 3.86 SLAPKK53 pKa = 10.04 LADD56 pKa = 3.12 SGVFAASAAVVMWAWFYY73 pKa = 11.39 LPEE76 pKa = 4.93 RR77 pKa = 11.84 LPKK80 pKa = 10.42 SYY82 pKa = 10.54 GKK84 pKa = 9.77 WIGEE88 pKa = 4.12 VAKK91 pKa = 10.63 VDD93 pKa = 3.53 SRR95 pKa = 11.84 LIEE98 pKa = 3.86 ALRR101 pKa = 11.84 RR102 pKa = 11.84 ARR104 pKa = 11.84 RR105 pKa = 11.84 GVFVYY110 pKa = 10.28 GKK112 pKa = 8.77 DD113 pKa = 3.14 TGQAPLLEE121 pKa = 4.73 SMCKK125 pKa = 10.29 DD126 pKa = 3.55 YY127 pKa = 10.96 GWPIEE132 pKa = 4.14 WGDD135 pKa = 3.6 PSKK138 pKa = 9.97 TIPIPCEE145 pKa = 3.88 MVHH148 pKa = 6.01 MSCGPNCEE156 pKa = 3.69 KK157 pKa = 10.72 HH158 pKa = 5.65 AVSRR162 pKa = 11.84 FARR165 pKa = 11.84 TFGFACATYY174 pKa = 10.13 IPLQIVFRR182 pKa = 11.84 LRR184 pKa = 11.84 RR185 pKa = 11.84 LKK187 pKa = 10.75 SVLSLRR193 pKa = 11.84 RR194 pKa = 11.84 AVSDD198 pKa = 3.34 AMRR201 pKa = 11.84 SSAFLASFVSIFYY214 pKa = 10.75 YY215 pKa = 9.68 SVCLARR221 pKa = 11.84 TRR223 pKa = 11.84 IGPKK227 pKa = 9.14 IFPRR231 pKa = 11.84 NVVTPMMWDD240 pKa = 2.97 SGLCVGAGCLMCGWSILVEE259 pKa = 4.44 SPSKK263 pKa = 10.38 RR264 pKa = 11.84 QEE266 pKa = 3.62 LALFVAPRR274 pKa = 11.84 AAATVLPRR282 pKa = 11.84 FYY284 pKa = 10.89 DD285 pKa = 2.98 KK286 pKa = 10.71 QYY288 pKa = 10.85 QYY290 pKa = 11.27 RR291 pKa = 11.84 EE292 pKa = 4.05 RR293 pKa = 11.84 IAFAVSAALLLTCLQEE309 pKa = 4.05 RR310 pKa = 11.84 PGMVRR315 pKa = 11.84 GVFGRR320 pKa = 11.84 IATSVLKK327 pKa = 10.85
Molecular weight: 36.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.516
IPC_protein 10.101
Toseland 10.277
ProMoST 10.058
Dawson 10.452
Bjellqvist 10.204
Wikipedia 10.657
Rodwell 10.657
Grimsley 10.526
Solomon 10.511
Lehninger 10.482
Nozaki 10.35
DTASelect 10.175
Thurlkill 10.321
EMBOSS 10.687
Sillero 10.394
Patrickios 10.262
IPC_peptide 10.511
IPC2_peptide 9.472
IPC2.peptide.svr19 8.468
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12090
0
12090
6134843
10
7745
507.4
56.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.269 ± 0.017
1.311 ± 0.009
5.628 ± 0.014
6.101 ± 0.024
3.821 ± 0.015
6.796 ± 0.02
2.448 ± 0.009
5.165 ± 0.015
4.585 ± 0.018
9.225 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.159 ± 0.008
3.735 ± 0.009
5.836 ± 0.021
4.033 ± 0.013
5.959 ± 0.019
8.225 ± 0.02
5.953 ± 0.015
6.277 ± 0.015
1.526 ± 0.009
2.948 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here