Dyella choica
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4604 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432M1D1|A0A432M1D1_9GAMM Uncharacterized protein OS=Dyella choica OX=1927959 GN=EKH80_19270 PE=4 SV=1
MM1 pKa = 6.98 NQISPEE7 pKa = 3.46 ITLRR11 pKa = 11.84 KK12 pKa = 8.02 WMCVVCGFIYY22 pKa = 10.65 DD23 pKa = 3.97 EE24 pKa = 5.19 AEE26 pKa = 4.0 GLPDD30 pKa = 3.7 EE31 pKa = 5.91 GIDD34 pKa = 4.31 PGTRR38 pKa = 11.84 WEE40 pKa = 4.73 DD41 pKa = 3.73 VPDD44 pKa = 3.14 TWTCPDD50 pKa = 3.66 CGATKK55 pKa = 10.55 SDD57 pKa = 3.59 FEE59 pKa = 4.35 MVEE62 pKa = 3.51 IDD64 pKa = 3.32
Molecular weight: 7.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.745
IPC_protein 3.668
Toseland 3.478
ProMoST 3.77
Dawson 3.668
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.503
Grimsley 3.389
Solomon 3.63
Lehninger 3.592
Nozaki 3.795
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.617
Sillero 3.795
Patrickios 1.85
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|A0A3S0Q4T0|A0A3S0Q4T0_9GAMM Glycosyl hydrolase OS=Dyella choica OX=1927959 GN=EKH80_09465 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.06 LKK11 pKa = 10.38 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATADD26 pKa = 3.35 GRR28 pKa = 11.84 KK29 pKa = 9.36 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.75 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LIPP44 pKa = 4.02
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4604
0
4604
1582698
19
7174
343.8
37.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.932 ± 0.049
0.902 ± 0.014
5.41 ± 0.03
4.924 ± 0.048
3.412 ± 0.024
8.259 ± 0.056
2.549 ± 0.025
4.428 ± 0.023
2.957 ± 0.033
10.758 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.301 ± 0.018
3.121 ± 0.051
5.181 ± 0.034
4.231 ± 0.027
6.634 ± 0.064
6.074 ± 0.055
5.396 ± 0.074
7.293 ± 0.027
1.522 ± 0.016
2.716 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here