Streptomyces phage Nishikigoi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CPX6|A0A411CPX6_9CAUD Uncharacterized protein OS=Streptomyces phage Nishikigoi OX=2510526 GN=76 PE=4 SV=1
MM1 pKa = 7.67SDD3 pKa = 3.07IEE5 pKa = 4.36ALGSALADD13 pKa = 3.64LAGVFGDD20 pKa = 5.55GMTADD25 pKa = 3.82HH26 pKa = 6.82TGSSFTCSEE35 pKa = 4.2ADD37 pKa = 3.42TIARR41 pKa = 11.84VLVLAGHH48 pKa = 6.96KK49 pKa = 10.04DD50 pKa = 3.39AAEE53 pKa = 4.14TWLHH57 pKa = 4.99GHH59 pKa = 7.33ADD61 pKa = 3.74GDD63 pKa = 4.3DD64 pKa = 4.29GGDD67 pKa = 3.35SHH69 pKa = 6.81YY70 pKa = 10.88HH71 pKa = 5.91YY72 pKa = 10.88DD73 pKa = 4.67DD74 pKa = 4.59EE75 pKa = 5.35EE76 pKa = 5.63DD77 pKa = 4.32PEE79 pKa = 5.45DD80 pKa = 3.6EE81 pKa = 4.38GRR83 pKa = 11.84PMTEE87 pKa = 3.94GEE89 pKa = 4.09VAEE92 pKa = 5.46YY93 pKa = 9.59VAEE96 pKa = 4.12WLSS99 pKa = 3.34

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CPU0|A0A411CPU0_9CAUD Deoxycytidylate deaminase OS=Streptomyces phage Nishikigoi OX=2510526 GN=36 PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 10.33RR3 pKa = 11.84GFTSEE8 pKa = 3.75RR9 pKa = 11.84DD10 pKa = 3.31AEE12 pKa = 4.34KK13 pKa = 11.01ALGRR17 pKa = 11.84AQAKK21 pKa = 10.07RR22 pKa = 11.84SRR24 pKa = 11.84QGQARR29 pKa = 11.84GTMRR33 pKa = 11.84GLKK36 pKa = 9.81VEE38 pKa = 4.14SRR40 pKa = 11.84FYY42 pKa = 10.29EE43 pKa = 4.44CEE45 pKa = 3.65HH46 pKa = 6.7GSFHH50 pKa = 6.37LTSEE54 pKa = 4.18SRR56 pKa = 11.84ASYY59 pKa = 9.59EE60 pKa = 3.89NRR62 pKa = 11.84VGAWW66 pKa = 2.94

Molecular weight: 7.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

15483

43

1301

188.8

20.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.277 ± 0.366

0.749 ± 0.115

6.452 ± 0.225

7.24 ± 0.383

3.042 ± 0.177

8.312 ± 0.276

2.054 ± 0.194

4.366 ± 0.224

4.67 ± 0.278

8.345 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.183 ± 0.132

2.738 ± 0.146

4.695 ± 0.24

3.436 ± 0.167

6.51 ± 0.358

5.936 ± 0.299

6.258 ± 0.273

7.137 ± 0.175

1.757 ± 0.114

2.842 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski