Amino acid dipepetide frequency for Streptomyces phage Nishikigoi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.143AlaAla: 12.143 ± 1.157
0.517AlaCys: 0.517 ± 0.176
8.914AlaAsp: 8.914 ± 0.63
8.849AlaGlu: 8.849 ± 0.852
3.488AlaPhe: 3.488 ± 0.487
8.72AlaGly: 8.72 ± 0.785
2.067AlaHis: 2.067 ± 0.396
4.909AlaIle: 4.909 ± 0.657
6.136AlaLys: 6.136 ± 0.779
10.851AlaLeu: 10.851 ± 1.015
2.648AlaMet: 2.648 ± 0.393
2.842AlaAsn: 2.842 ± 0.443
4.651AlaPro: 4.651 ± 0.566
2.842AlaGln: 2.842 ± 0.559
6.976AlaArg: 6.976 ± 0.761
5.942AlaSer: 5.942 ± 0.654
6.265AlaThr: 6.265 ± 0.647
7.816AlaVal: 7.816 ± 0.694
2.584AlaTrp: 2.584 ± 0.344
3.553AlaTyr: 3.553 ± 0.477
0.0AlaXaa: 0.0 ± 0.0
Cys
0.775CysAla: 0.775 ± 0.264
0.065CysCys: 0.065 ± 0.059
0.581CysAsp: 0.581 ± 0.2
0.323CysGlu: 0.323 ± 0.15
0.129CysPhe: 0.129 ± 0.101
0.517CysGly: 0.517 ± 0.165
0.323CysHis: 0.323 ± 0.144
0.517CysIle: 0.517 ± 0.195
0.452CysLys: 0.452 ± 0.25
0.517CysLeu: 0.517 ± 0.184
0.065CysMet: 0.065 ± 0.061
0.194CysAsn: 0.194 ± 0.114
0.646CysPro: 0.646 ± 0.247
0.258CysGln: 0.258 ± 0.118
0.388CysArg: 0.388 ± 0.181
0.646CysSer: 0.646 ± 0.205
0.452CysThr: 0.452 ± 0.172
0.258CysVal: 0.258 ± 0.16
0.194CysTrp: 0.194 ± 0.113
0.194CysTyr: 0.194 ± 0.117
0.0CysXaa: 0.0 ± 0.0
Asp
7.428AspAla: 7.428 ± 0.641
0.581AspCys: 0.581 ± 0.19
4.521AspAsp: 4.521 ± 0.506
5.49AspGlu: 5.49 ± 0.691
1.55AspPhe: 1.55 ± 0.322
6.524AspGly: 6.524 ± 0.568
1.292AspHis: 1.292 ± 0.283
2.39AspIle: 2.39 ± 0.355
2.454AspLys: 2.454 ± 0.427
5.555AspLeu: 5.555 ± 0.589
1.486AspMet: 1.486 ± 0.287
1.292AspAsn: 1.292 ± 0.221
3.94AspPro: 3.94 ± 0.517
2.132AspGln: 2.132 ± 0.32
3.1AspArg: 3.1 ± 0.481
3.94AspSer: 3.94 ± 0.524
3.294AspThr: 3.294 ± 0.495
4.651AspVal: 4.651 ± 0.584
1.615AspTrp: 1.615 ± 0.347
1.292AspTyr: 1.292 ± 0.247
0.0AspXaa: 0.0 ± 0.0
Glu
7.88GluAla: 7.88 ± 0.907
0.84GluCys: 0.84 ± 0.237
4.198GluAsp: 4.198 ± 0.487
5.749GluGlu: 5.749 ± 0.873
2.584GluPhe: 2.584 ± 0.365
6.072GluGly: 6.072 ± 0.538
1.615GluHis: 1.615 ± 0.362
3.617GluIle: 3.617 ± 0.466
2.842GluLys: 2.842 ± 0.388
7.557GluLeu: 7.557 ± 0.77
0.969GluMet: 0.969 ± 0.211
1.679GluAsn: 1.679 ± 0.315
2.842GluPro: 2.842 ± 0.666
3.294GluGln: 3.294 ± 0.461
4.392GluArg: 4.392 ± 0.665
2.842GluSer: 2.842 ± 0.465
4.328GluThr: 4.328 ± 0.729
5.103GluVal: 5.103 ± 0.636
1.292GluTrp: 1.292 ± 0.299
2.713GluTyr: 2.713 ± 0.522
0.0GluXaa: 0.0 ± 0.0
Phe
3.875PheAla: 3.875 ± 0.576
0.129PheCys: 0.129 ± 0.086
2.196PheAsp: 2.196 ± 0.291
2.519PheGlu: 2.519 ± 0.351
0.711PhePhe: 0.711 ± 0.278
3.165PheGly: 3.165 ± 0.357
0.711PheHis: 0.711 ± 0.327
1.163PheIle: 1.163 ± 0.287
0.581PheLys: 0.581 ± 0.157
1.744PheLeu: 1.744 ± 0.497
1.033PheMet: 1.033 ± 0.268
1.292PheAsn: 1.292 ± 0.268
1.098PhePro: 1.098 ± 0.292
1.033PheGln: 1.033 ± 0.241
1.873PheArg: 1.873 ± 0.347
1.421PheSer: 1.421 ± 0.314
2.39PheThr: 2.39 ± 0.436
2.067PheVal: 2.067 ± 0.45
0.517PheTrp: 0.517 ± 0.156
0.904PheTyr: 0.904 ± 0.226
0.0PheXaa: 0.0 ± 0.0
Gly
8.397GlyAla: 8.397 ± 0.897
0.323GlyCys: 0.323 ± 0.135
4.78GlyAsp: 4.78 ± 0.69
5.232GlyGlu: 5.232 ± 0.472
3.1GlyPhe: 3.1 ± 0.498
5.878GlyGly: 5.878 ± 0.804
2.132GlyHis: 2.132 ± 0.411
3.423GlyIle: 3.423 ± 0.871
5.296GlyLys: 5.296 ± 0.765
6.847GlyLeu: 6.847 ± 0.733
1.227GlyMet: 1.227 ± 0.246
2.907GlyAsn: 2.907 ± 0.354
4.005GlyPro: 4.005 ± 0.551
3.423GlyGln: 3.423 ± 0.501
5.619GlyArg: 5.619 ± 0.835
5.038GlySer: 5.038 ± 0.68
5.038GlyThr: 5.038 ± 0.631
6.136GlyVal: 6.136 ± 0.581
1.873GlyTrp: 1.873 ± 0.4
2.454GlyTyr: 2.454 ± 0.377
0.0GlyXaa: 0.0 ± 0.0
His
2.002HisAla: 2.002 ± 0.319
0.129HisCys: 0.129 ± 0.085
0.969HisAsp: 0.969 ± 0.23
1.227HisGlu: 1.227 ± 0.297
1.033HisPhe: 1.033 ± 0.28
1.873HisGly: 1.873 ± 0.329
0.646HisHis: 0.646 ± 0.207
0.646HisIle: 0.646 ± 0.231
0.517HisLys: 0.517 ± 0.167
1.938HisLeu: 1.938 ± 0.356
0.323HisMet: 0.323 ± 0.14
0.388HisAsn: 0.388 ± 0.167
0.969HisPro: 0.969 ± 0.235
0.84HisGln: 0.84 ± 0.307
1.615HisArg: 1.615 ± 0.394
1.421HisSer: 1.421 ± 0.429
1.55HisThr: 1.55 ± 0.285
1.227HisVal: 1.227 ± 0.33
0.517HisTrp: 0.517 ± 0.191
0.711HisTyr: 0.711 ± 0.251
0.0HisXaa: 0.0 ± 0.0
Ile
4.78IleAla: 4.78 ± 0.588
0.323IleCys: 0.323 ± 0.147
3.682IleAsp: 3.682 ± 0.587
4.263IleGlu: 4.263 ± 0.456
1.55IlePhe: 1.55 ± 0.304
3.488IleGly: 3.488 ± 0.541
0.84IleHis: 0.84 ± 0.168
1.938IleIle: 1.938 ± 0.419
2.196IleLys: 2.196 ± 0.578
2.777IleLeu: 2.777 ± 0.502
0.517IleMet: 0.517 ± 0.27
1.486IleAsn: 1.486 ± 0.269
1.873IlePro: 1.873 ± 0.317
1.486IleGln: 1.486 ± 0.261
3.1IleArg: 3.1 ± 0.542
1.873IleSer: 1.873 ± 0.403
2.584IleThr: 2.584 ± 0.425
3.553IleVal: 3.553 ± 0.435
0.258IleTrp: 0.258 ± 0.126
1.098IleTyr: 1.098 ± 0.303
0.0IleXaa: 0.0 ± 0.0
Lys
4.521LysAla: 4.521 ± 0.68
0.388LysCys: 0.388 ± 0.124
2.713LysAsp: 2.713 ± 0.436
2.584LysGlu: 2.584 ± 0.463
1.033LysPhe: 1.033 ± 0.222
5.296LysGly: 5.296 ± 0.625
0.711LysHis: 0.711 ± 0.241
2.132LysIle: 2.132 ± 0.486
3.423LysLys: 3.423 ± 0.606
4.134LysLeu: 4.134 ± 0.603
0.969LysMet: 0.969 ± 0.284
0.969LysAsn: 0.969 ± 0.233
2.519LysPro: 2.519 ± 0.576
1.679LysGln: 1.679 ± 0.262
3.165LysArg: 3.165 ± 0.534
2.648LysSer: 2.648 ± 0.482
2.325LysThr: 2.325 ± 0.36
3.553LysVal: 3.553 ± 0.54
0.452LysTrp: 0.452 ± 0.181
1.486LysTyr: 1.486 ± 0.309
0.0LysXaa: 0.0 ± 0.0
Leu
11.626LeuAla: 11.626 ± 0.947
0.711LeuCys: 0.711 ± 0.231
4.909LeuAsp: 4.909 ± 0.656
4.521LeuGlu: 4.521 ± 0.547
1.679LeuPhe: 1.679 ± 0.443
7.493LeuGly: 7.493 ± 0.934
1.615LeuHis: 1.615 ± 0.315
3.553LeuIle: 3.553 ± 0.479
3.488LeuLys: 3.488 ± 0.621
6.265LeuLeu: 6.265 ± 0.715
1.744LeuMet: 1.744 ± 0.419
3.1LeuAsn: 3.1 ± 0.395
4.586LeuPro: 4.586 ± 0.575
2.584LeuGln: 2.584 ± 0.404
5.749LeuArg: 5.749 ± 0.818
5.878LeuSer: 5.878 ± 0.687
5.361LeuThr: 5.361 ± 0.493
5.361LeuVal: 5.361 ± 0.655
0.904LeuTrp: 0.904 ± 0.213
2.325LeuTyr: 2.325 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
4.069MetAla: 4.069 ± 0.455
0.194MetCys: 0.194 ± 0.102
1.486MetAsp: 1.486 ± 0.26
1.292MetGlu: 1.292 ± 0.274
0.517MetPhe: 0.517 ± 0.184
1.163MetGly: 1.163 ± 0.314
0.581MetHis: 0.581 ± 0.171
1.033MetIle: 1.033 ± 0.261
0.84MetLys: 0.84 ± 0.224
1.938MetLeu: 1.938 ± 0.324
0.323MetMet: 0.323 ± 0.141
0.258MetAsn: 0.258 ± 0.122
0.904MetPro: 0.904 ± 0.233
0.646MetGln: 0.646 ± 0.153
1.421MetArg: 1.421 ± 0.375
1.615MetSer: 1.615 ± 0.313
1.744MetThr: 1.744 ± 0.418
1.033MetVal: 1.033 ± 0.245
0.452MetTrp: 0.452 ± 0.189
0.323MetTyr: 0.323 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
2.971AsnAla: 2.971 ± 0.413
0.646AsnCys: 0.646 ± 0.223
1.227AsnAsp: 1.227 ± 0.258
2.325AsnGlu: 2.325 ± 0.377
0.969AsnPhe: 0.969 ± 0.255
2.713AsnGly: 2.713 ± 0.325
0.517AsnHis: 0.517 ± 0.182
1.227AsnIle: 1.227 ± 0.224
1.292AsnLys: 1.292 ± 0.226
2.519AsnLeu: 2.519 ± 0.453
0.517AsnMet: 0.517 ± 0.167
0.646AsnAsn: 0.646 ± 0.203
1.227AsnPro: 1.227 ± 0.304
0.904AsnGln: 0.904 ± 0.258
1.55AsnArg: 1.55 ± 0.21
1.486AsnSer: 1.486 ± 0.35
1.615AsnThr: 1.615 ± 0.32
1.679AsnVal: 1.679 ± 0.326
0.517AsnTrp: 0.517 ± 0.16
0.84AsnTyr: 0.84 ± 0.203
0.0AsnXaa: 0.0 ± 0.0
Pro
5.296ProAla: 5.296 ± 0.791
0.452ProCys: 0.452 ± 0.181
3.553ProAsp: 3.553 ± 0.477
3.94ProGlu: 3.94 ± 0.739
0.969ProPhe: 0.969 ± 0.252
3.553ProGly: 3.553 ± 0.395
0.388ProHis: 0.388 ± 0.149
2.132ProIle: 2.132 ± 0.377
3.036ProLys: 3.036 ± 0.503
3.165ProLeu: 3.165 ± 0.524
1.292ProMet: 1.292 ± 0.255
0.969ProAsn: 0.969 ± 0.232
2.132ProPro: 2.132 ± 0.335
1.356ProGln: 1.356 ± 0.279
3.23ProArg: 3.23 ± 0.525
2.907ProSer: 2.907 ± 0.534
2.907ProThr: 2.907 ± 0.477
3.553ProVal: 3.553 ± 0.633
0.904ProTrp: 0.904 ± 0.227
1.227ProTyr: 1.227 ± 0.28
0.0ProXaa: 0.0 ± 0.0
Gln
4.715GlnAla: 4.715 ± 0.552
0.194GlnCys: 0.194 ± 0.103
1.615GlnAsp: 1.615 ± 0.407
2.325GlnGlu: 2.325 ± 0.484
1.098GlnPhe: 1.098 ± 0.267
2.132GlnGly: 2.132 ± 0.339
0.388GlnHis: 0.388 ± 0.171
1.938GlnIle: 1.938 ± 0.303
1.55GlnLys: 1.55 ± 0.322
3.165GlnLeu: 3.165 ± 0.666
0.969GlnMet: 0.969 ± 0.279
0.646GlnAsn: 0.646 ± 0.172
1.615GlnPro: 1.615 ± 0.28
1.163GlnGln: 1.163 ± 0.241
2.713GlnArg: 2.713 ± 0.38
2.39GlnSer: 2.39 ± 0.358
1.615GlnThr: 1.615 ± 0.325
2.713GlnVal: 2.713 ± 0.422
0.581GlnTrp: 0.581 ± 0.245
0.84GlnTyr: 0.84 ± 0.213
0.0GlnXaa: 0.0 ± 0.0
Arg
6.265ArgAla: 6.265 ± 0.819
0.646ArgCys: 0.646 ± 0.182
4.069ArgAsp: 4.069 ± 0.508
5.232ArgGlu: 5.232 ± 0.817
2.325ArgPhe: 2.325 ± 0.374
4.392ArgGly: 4.392 ± 0.701
1.615ArgHis: 1.615 ± 0.357
2.971ArgIle: 2.971 ± 0.371
3.294ArgLys: 3.294 ± 0.495
5.426ArgLeu: 5.426 ± 0.612
1.809ArgMet: 1.809 ± 0.4
2.002ArgAsn: 2.002 ± 0.353
2.777ArgPro: 2.777 ± 0.384
2.842ArgGln: 2.842 ± 0.498
4.78ArgArg: 4.78 ± 0.672
3.553ArgSer: 3.553 ± 0.464
3.811ArgThr: 3.811 ± 0.619
4.78ArgVal: 4.78 ± 0.549
0.775ArgTrp: 0.775 ± 0.226
1.744ArgTyr: 1.744 ± 0.323
0.0ArgXaa: 0.0 ± 0.0
Ser
6.072SerAla: 6.072 ± 0.654
0.129SerCys: 0.129 ± 0.088
3.23SerAsp: 3.23 ± 0.413
4.069SerGlu: 4.069 ± 0.429
1.938SerPhe: 1.938 ± 0.294
5.684SerGly: 5.684 ± 0.648
1.486SerHis: 1.486 ± 0.46
2.971SerIle: 2.971 ± 0.414
2.454SerLys: 2.454 ± 0.402
5.038SerLeu: 5.038 ± 0.579
1.356SerMet: 1.356 ± 0.289
1.356SerAsn: 1.356 ± 0.272
2.067SerPro: 2.067 ± 0.333
2.002SerGln: 2.002 ± 0.382
4.005SerArg: 4.005 ± 0.484
4.134SerSer: 4.134 ± 0.652
4.457SerThr: 4.457 ± 0.694
4.198SerVal: 4.198 ± 0.502
0.904SerTrp: 0.904 ± 0.264
1.809SerTyr: 1.809 ± 0.308
0.0SerXaa: 0.0 ± 0.0
Thr
7.299ThrAla: 7.299 ± 0.666
0.452ThrCys: 0.452 ± 0.198
3.875ThrAsp: 3.875 ± 0.495
3.553ThrGlu: 3.553 ± 0.523
2.325ThrPhe: 2.325 ± 0.538
5.619ThrGly: 5.619 ± 0.606
1.163ThrHis: 1.163 ± 0.293
2.519ThrIle: 2.519 ± 0.441
1.55ThrLys: 1.55 ± 0.26
4.392ThrLeu: 4.392 ± 0.536
1.421ThrMet: 1.421 ± 0.305
1.615ThrAsn: 1.615 ± 0.359
3.682ThrPro: 3.682 ± 0.681
2.132ThrGln: 2.132 ± 0.344
3.294ThrArg: 3.294 ± 0.476
4.78ThrSer: 4.78 ± 0.814
3.682ThrThr: 3.682 ± 0.458
5.878ThrVal: 5.878 ± 0.654
0.969ThrTrp: 0.969 ± 0.256
2.39ThrTyr: 2.39 ± 0.378
0.0ThrXaa: 0.0 ± 0.0
Val
7.622ValAla: 7.622 ± 0.556
0.452ValCys: 0.452 ± 0.163
4.198ValAsp: 4.198 ± 0.407
5.49ValGlu: 5.49 ± 0.73
2.132ValPhe: 2.132 ± 0.301
5.167ValGly: 5.167 ± 0.563
1.292ValHis: 1.292 ± 0.337
3.23ValIle: 3.23 ± 0.491
3.553ValLys: 3.553 ± 0.439
6.007ValLeu: 6.007 ± 0.821
1.873ValMet: 1.873 ± 0.29
2.325ValAsn: 2.325 ± 0.357
3.617ValPro: 3.617 ± 0.548
2.777ValGln: 2.777 ± 0.359
4.586ValArg: 4.586 ± 0.603
3.682ValSer: 3.682 ± 0.474
5.942ValThr: 5.942 ± 0.68
4.844ValVal: 4.844 ± 0.644
1.421ValTrp: 1.421 ± 0.271
1.163ValTyr: 1.163 ± 0.289
0.0ValXaa: 0.0 ± 0.0
Trp
2.261TrpAla: 2.261 ± 0.445
0.194TrpCys: 0.194 ± 0.123
1.356TrpAsp: 1.356 ± 0.277
1.098TrpGlu: 1.098 ± 0.235
0.452TrpPhe: 0.452 ± 0.156
1.163TrpGly: 1.163 ± 0.32
0.517TrpHis: 0.517 ± 0.178
0.258TrpIle: 0.258 ± 0.119
0.969TrpLys: 0.969 ± 0.238
1.356TrpLeu: 1.356 ± 0.273
0.646TrpMet: 0.646 ± 0.197
0.581TrpAsn: 0.581 ± 0.175
0.646TrpPro: 0.646 ± 0.185
0.323TrpGln: 0.323 ± 0.183
1.356TrpArg: 1.356 ± 0.218
1.292TrpSer: 1.292 ± 0.234
1.615TrpThr: 1.615 ± 0.29
1.033TrpVal: 1.033 ± 0.263
0.194TrpTrp: 0.194 ± 0.121
0.258TrpTyr: 0.258 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.777TyrAla: 2.777 ± 0.404
0.129TyrCys: 0.129 ± 0.096
2.454TyrAsp: 2.454 ± 0.493
2.325TyrGlu: 2.325 ± 0.423
0.84TyrPhe: 0.84 ± 0.176
2.519TyrGly: 2.519 ± 0.422
0.646TyrHis: 0.646 ± 0.27
1.098TyrIle: 1.098 ± 0.264
0.775TyrLys: 0.775 ± 0.224
2.261TyrLeu: 2.261 ± 0.403
0.581TyrMet: 0.581 ± 0.17
0.84TyrAsn: 0.84 ± 0.182
1.163TyrPro: 1.163 ± 0.284
0.646TyrGln: 0.646 ± 0.18
2.196TyrArg: 2.196 ± 0.471
1.873TyrSer: 1.873 ± 0.401
1.615TyrThr: 1.615 ± 0.33
1.938TyrVal: 1.938 ± 0.319
0.646TyrTrp: 0.646 ± 0.229
1.098TyrTyr: 1.098 ± 0.299
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (15483 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski