Microbacterium paludicola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3006 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9FSB3|A0A4Y9FSB3_9MICO Aminodeoxychorismate lyase OS=Microbacterium paludicola OX=300019 GN=E4U02_12240 PE=4 SV=1
MM1 pKa = 6.84VRR3 pKa = 11.84SRR5 pKa = 11.84KK6 pKa = 10.0LIGALALTGAAALTLAGCATGGGGTATEE34 pKa = 4.92DD35 pKa = 3.72PSAGPAEE42 pKa = 4.43PGGDD46 pKa = 3.73LSLKK50 pKa = 10.1IATALPVTGSLAFLGPPEE68 pKa = 4.08IQGVNYY74 pKa = 9.83AQSLINEE81 pKa = 4.39YY82 pKa = 9.7TEE84 pKa = 4.19EE85 pKa = 4.15TGLEE89 pKa = 4.08LDD91 pKa = 4.23VVHH94 pKa = 7.05GDD96 pKa = 5.45SGDD99 pKa = 4.01LDD101 pKa = 3.69NKK103 pKa = 10.28KK104 pKa = 10.55YY105 pKa = 10.36EE106 pKa = 4.19SEE108 pKa = 3.93IPRR111 pKa = 11.84LLSEE115 pKa = 5.09DD116 pKa = 3.25PAAIIGAASSGVSLQFIDD134 pKa = 3.62QVVGAGVIQFSPANTSDD151 pKa = 3.9AFTTYY156 pKa = 10.99DD157 pKa = 3.97DD158 pKa = 3.37NGLYY162 pKa = 10.42FRR164 pKa = 11.84TAPSDD169 pKa = 3.6VLQGEE174 pKa = 4.35VHH176 pKa = 6.59GNFLGEE182 pKa = 4.92LGHH185 pKa = 5.7QTLGLIVLNDD195 pKa = 3.6AYY197 pKa = 10.34GTGLAKK203 pKa = 10.77YY204 pKa = 8.47ITEE207 pKa = 4.17AFEE210 pKa = 4.32GAGGEE215 pKa = 4.5VVASEE220 pKa = 4.39TFNPGDD226 pKa = 3.6TSFDD230 pKa = 3.61SQIAAVLAEE239 pKa = 5.17DD240 pKa = 4.36PDD242 pKa = 5.98AITVISFDD250 pKa = 3.56EE251 pKa = 4.73AKK253 pKa = 10.12TILPSLIEE261 pKa = 4.12KK262 pKa = 10.78GFDD265 pKa = 3.25AEE267 pKa = 4.85NLFLVDD273 pKa = 4.63GNMAAYY279 pKa = 10.28DD280 pKa = 3.75KK281 pKa = 10.96DD282 pKa = 4.19FPAGLLEE289 pKa = 4.51GAKK292 pKa = 8.77GTYY295 pKa = 9.39PGPTPDD301 pKa = 3.18QVQEE305 pKa = 4.19FVDD308 pKa = 3.84GLNAFVEE315 pKa = 4.64EE316 pKa = 4.76SGTEE320 pKa = 3.8ALSDD324 pKa = 3.39YY325 pKa = 10.26TYY327 pKa = 11.26APEE330 pKa = 5.11SFDD333 pKa = 3.71AVNLIALASLAAQSTEE349 pKa = 3.83PADD352 pKa = 3.39IAEE355 pKa = 4.49KK356 pKa = 10.35LVEE359 pKa = 4.02VSGGSGEE366 pKa = 4.46GEE368 pKa = 4.2KK369 pKa = 9.9CTSFADD375 pKa = 3.8CADD378 pKa = 4.17IILGGGVADD387 pKa = 3.97YY388 pKa = 10.9DD389 pKa = 4.25GASSPVTFDD398 pKa = 3.31EE399 pKa = 4.97VGDD402 pKa = 3.89PTEE405 pKa = 4.02GMINIYY411 pKa = 10.38EE412 pKa = 4.03YY413 pKa = 11.22GADD416 pKa = 3.43NKK418 pKa = 10.19YY419 pKa = 10.76VPYY422 pKa = 10.56EE423 pKa = 3.99GG424 pKa = 4.4

Molecular weight: 43.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9FU96|A0A4Y9FU96_9MICO YhgE/Pip domain-containing protein OS=Microbacterium paludicola OX=300019 GN=E4U02_08900 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3006

0

3006

972793

32

2067

323.6

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.693 ± 0.061

0.489 ± 0.01

6.322 ± 0.035

6.129 ± 0.042

3.038 ± 0.029

8.938 ± 0.041

2.084 ± 0.022

4.567 ± 0.032

1.966 ± 0.034

10.038 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.777 ± 0.018

1.813 ± 0.024

5.517 ± 0.032

2.772 ± 0.023

7.723 ± 0.061

5.122 ± 0.029

5.828 ± 0.036

8.67 ± 0.045

1.536 ± 0.019

1.975 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski