Amino acid dipepetide frequency for Microbacterium paludicola

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.861AlaAla: 20.861 ± 0.231	0.678AlaCys: 0.678 ± 0.032	8.473AlaAsp: 8.473 ± 0.11	9.242AlaGlu: 9.242 ± 0.119	3.936AlaPhe: 3.936 ± 0.064	12.179AlaGly: 12.179 ± 0.137	2.69AlaHis: 2.69 ± 0.054	6.226AlaIle: 6.226 ± 0.078	2.717AlaLys: 2.717 ± 0.077	14.314AlaLeu: 14.314 ± 0.16	2.72AlaMet: 2.72 ± 0.055	2.167AlaAsn: 2.167 ± 0.05	6.905AlaPro: 6.905 ± 0.101	4.005AlaGln: 4.005 ± 0.065	9.987AlaArg: 9.987 ± 0.136	6.626AlaSer: 6.626 ± 0.084	7.323AlaThr: 7.323 ± 0.092	11.323AlaVal: 11.323 ± 0.12	2.028AlaTrp: 2.028 ± 0.051	2.526AlaTyr: 2.526 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.691CysAla: 0.691 ± 0.029	0.05CysCys: 0.05 ± 0.008	0.318CysAsp: 0.318 ± 0.016	0.278CysGlu: 0.278 ± 0.018	0.153CysPhe: 0.153 ± 0.014	0.547CysGly: 0.547 ± 0.028	0.117CysHis: 0.117 ± 0.009	0.17CysIle: 0.17 ± 0.014	0.059CysLys: 0.059 ± 0.01	0.431CysLeu: 0.431 ± 0.024	0.076CysMet: 0.076 ± 0.01	0.086CysAsn: 0.086 ± 0.01	0.278CysPro: 0.278 ± 0.018	0.117CysGln: 0.117 ± 0.012	0.37CysArg: 0.37 ± 0.023	0.268CysSer: 0.268 ± 0.017	0.315CysThr: 0.315 ± 0.019	0.392CysVal: 0.392 ± 0.02	0.068CysTrp: 0.068 ± 0.009	0.109CysTyr: 0.109 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	9.267AspAla: 9.267 ± 0.111	0.246AspCys: 0.246 ± 0.018	4.345AspAsp: 4.345 ± 0.09	4.864AspGlu: 4.864 ± 0.091	1.736AspPhe: 1.736 ± 0.036	6.227AspGly: 6.227 ± 0.091	1.301AspHis: 1.301 ± 0.038	2.52AspIle: 2.52 ± 0.05	1.051AspLys: 1.051 ± 0.04	6.229AspLeu: 6.229 ± 0.087	0.853AspMet: 0.853 ± 0.029	0.883AspAsn: 0.883 ± 0.034	4.498AspPro: 4.498 ± 0.075	1.446AspGln: 1.446 ± 0.04	4.633AspArg: 4.633 ± 0.087	2.287AspSer: 2.287 ± 0.05	2.898AspThr: 2.898 ± 0.062	5.738AspVal: 5.738 ± 0.082	0.957AspTrp: 0.957 ± 0.036	1.236AspTyr: 1.236 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	8.025GluAla: 8.025 ± 0.104	0.263GluCys: 0.263 ± 0.017	3.154GluAsp: 3.154 ± 0.057	3.41GluGlu: 3.41 ± 0.063	1.779GluPhe: 1.779 ± 0.043	4.685GluGly: 4.685 ± 0.078	1.61GluHis: 1.61 ± 0.041	2.896GluIle: 2.896 ± 0.053	1.54GluLys: 1.54 ± 0.045	6.847GluLeu: 6.847 ± 0.097	0.951GluMet: 0.951 ± 0.034	1.275GluAsn: 1.275 ± 0.034	3.428GluPro: 3.428 ± 0.069	2.458GluGln: 2.458 ± 0.054	5.795GluArg: 5.795 ± 0.101	2.76GluSer: 2.76 ± 0.052	3.345GluThr: 3.345 ± 0.055	4.861GluVal: 4.861 ± 0.077	0.962GluTrp: 0.962 ± 0.032	1.248GluTyr: 1.248 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	4.352PheAla: 4.352 ± 0.068	0.156PheCys: 0.156 ± 0.012	2.323PheAsp: 2.323 ± 0.049	1.903PheGlu: 1.903 ± 0.049	1.006PhePhe: 1.006 ± 0.039	3.227PheGly: 3.227 ± 0.066	0.599PheHis: 0.599 ± 0.027	1.055PheIle: 1.055 ± 0.036	0.473PheLys: 0.473 ± 0.022	2.845PheLeu: 2.845 ± 0.067	0.483PheMet: 0.483 ± 0.022	0.65PheAsn: 0.65 ± 0.03	1.373PhePro: 1.373 ± 0.043	0.746PheGln: 0.746 ± 0.028	1.858PheArg: 1.858 ± 0.043	1.576PheSer: 1.576 ± 0.042	1.959PheThr: 1.959 ± 0.044	2.668PheVal: 2.668 ± 0.055	0.497PheTrp: 0.497 ± 0.027	0.634PheTyr: 0.634 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	10.982GlyAla: 10.982 ± 0.13	0.591GlyCys: 0.591 ± 0.026	5.322GlyAsp: 5.322 ± 0.086	5.493GlyGlu: 5.493 ± 0.077	3.279GlyPhe: 3.279 ± 0.067	7.684GlyGly: 7.684 ± 0.114	1.812GlyHis: 1.812 ± 0.046	4.755GlyIle: 4.755 ± 0.076	2.052GlyLys: 2.052 ± 0.046	8.636GlyLeu: 8.636 ± 0.101	1.996GlyMet: 1.996 ± 0.038	1.581GlyAsn: 1.581 ± 0.047	3.929GlyPro: 3.929 ± 0.069	2.388GlyGln: 2.388 ± 0.059	6.802GlyArg: 6.802 ± 0.102	5.033GlySer: 5.033 ± 0.074	5.265GlyThr: 5.265 ± 0.076	7.936GlyVal: 7.936 ± 0.102	1.673GlyTrp: 1.673 ± 0.044	2.169GlyTyr: 2.169 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.595HisAla: 2.595 ± 0.06	0.105HisCys: 0.105 ± 0.011	1.333HisAsp: 1.333 ± 0.038	1.261HisGlu: 1.261 ± 0.034	0.602HisPhe: 0.602 ± 0.026	2.067HisGly: 2.067 ± 0.043	0.634HisHis: 0.634 ± 0.028	0.817HisIle: 0.817 ± 0.032	0.275HisLys: 0.275 ± 0.016	2.08HisLeu: 2.08 ± 0.048	0.304HisMet: 0.304 ± 0.019	0.388HisAsn: 0.388 ± 0.02	1.58HisPro: 1.58 ± 0.048	0.467HisGln: 0.467 ± 0.019	1.801HisArg: 1.801 ± 0.052	0.957HisSer: 0.957 ± 0.031	1.031HisThr: 1.031 ± 0.034	1.823HisVal: 1.823 ± 0.046	0.308HisTrp: 0.308 ± 0.018	0.413HisTyr: 0.413 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.27IleAla: 7.27 ± 0.081	0.244IleCys: 0.244 ± 0.017	3.518IleAsp: 3.518 ± 0.059	3.184IleGlu: 3.184 ± 0.053	1.073IlePhe: 1.073 ± 0.039	4.646IleGly: 4.646 ± 0.075	0.797IleHis: 0.797 ± 0.032	1.617IleIle: 1.617 ± 0.047	0.74IleLys: 0.74 ± 0.032	4.056IleLeu: 4.056 ± 0.069	0.569IleMet: 0.569 ± 0.023	0.811IleAsn: 0.811 ± 0.029	2.402IlePro: 2.402 ± 0.052	1.078IleGln: 1.078 ± 0.032	3.147IleArg: 3.147 ± 0.058	2.082IleSer: 2.082 ± 0.041	2.679IleThr: 2.679 ± 0.065	4.495IleVal: 4.495 ± 0.076	0.5IleTrp: 0.5 ± 0.027	0.762IleTyr: 0.762 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	2.565LysAla: 2.565 ± 0.07	0.07LysCys: 0.07 ± 0.008	1.187LysAsp: 1.187 ± 0.044	0.939LysGlu: 0.939 ± 0.033	0.467LysPhe: 0.467 ± 0.021	1.604LysGly: 1.604 ± 0.049	0.466LysHis: 0.466 ± 0.022	0.882LysIle: 0.882 ± 0.035	0.71LysLys: 0.71 ± 0.032	1.863LysLeu: 1.863 ± 0.051	0.358LysMet: 0.358 ± 0.018	0.489LysAsn: 0.489 ± 0.025	1.252LysPro: 1.252 ± 0.043	0.674LysGln: 0.674 ± 0.026	1.569LysArg: 1.569 ± 0.054	1.017LysSer: 1.017 ± 0.036	1.303LysThr: 1.303 ± 0.039	1.622LysVal: 1.622 ± 0.049	0.223LysTrp: 0.223 ± 0.014	0.405LysTyr: 0.405 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.752LeuAla: 14.752 ± 0.171	0.472LeuCys: 0.472 ± 0.021	6.59LeuAsp: 6.59 ± 0.107	5.718LeuGlu: 5.718 ± 0.085	2.821LeuPhe: 2.821 ± 0.059	9.156LeuGly: 9.156 ± 0.097	1.988LeuHis: 1.988 ± 0.051	4.465LeuIle: 4.465 ± 0.078	1.765LeuLys: 1.765 ± 0.056	9.963LeuLeu: 9.963 ± 0.13	1.64LeuMet: 1.64 ± 0.045	1.686LeuAsn: 1.686 ± 0.045	5.637LeuPro: 5.637 ± 0.081	2.51LeuGln: 2.51 ± 0.052	7.904LeuArg: 7.904 ± 0.117	5.378LeuSer: 5.378 ± 0.079	6.105LeuThr: 6.105 ± 0.077	8.871LeuVal: 8.871 ± 0.112	1.32LeuTrp: 1.32 ± 0.047	1.639LeuTyr: 1.639 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.111MetAla: 2.111 ± 0.043	0.091MetCys: 0.091 ± 0.009	0.843MetAsp: 0.843 ± 0.03	0.63MetGlu: 0.63 ± 0.024	0.492MetPhe: 0.492 ± 0.021	1.314MetGly: 1.314 ± 0.043	0.369MetHis: 0.369 ± 0.02	0.882MetIle: 0.882 ± 0.031	0.408MetLys: 0.408 ± 0.02	1.87MetLeu: 1.87 ± 0.052	0.331MetMet: 0.331 ± 0.02	0.426MetAsn: 0.426 ± 0.019	1.13MetPro: 1.13 ± 0.034	0.554MetGln: 0.554 ± 0.024	1.499MetArg: 1.499 ± 0.04	1.383MetSer: 1.383 ± 0.035	1.637MetThr: 1.637 ± 0.046	1.25MetVal: 1.25 ± 0.038	0.253MetTrp: 0.253 ± 0.018	0.295MetTyr: 0.295 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.257AsnAla: 2.257 ± 0.047	0.102AsnCys: 0.102 ± 0.012	1.026AsnAsp: 1.026 ± 0.039	1.03AsnGlu: 1.03 ± 0.035	0.571AsnPhe: 0.571 ± 0.03	1.744AsnGly: 1.744 ± 0.05	0.345AsnHis: 0.345 ± 0.018	0.792AsnIle: 0.792 ± 0.031	0.392AsnLys: 0.392 ± 0.02	1.807AsnLeu: 1.807 ± 0.048	0.289AsnMet: 0.289 ± 0.017	0.392AsnAsn: 0.392 ± 0.024	1.479AsnPro: 1.479 ± 0.044	0.504AsnGln: 0.504 ± 0.023	1.23AsnArg: 1.23 ± 0.037	0.83AsnSer: 0.83 ± 0.031	1.029AsnThr: 1.029 ± 0.041	1.575AsnVal: 1.575 ± 0.044	0.281AsnTrp: 0.281 ± 0.017	0.458AsnTyr: 0.458 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.766ProAla: 7.766 ± 0.116	0.185ProCys: 0.185 ± 0.016	4.203ProAsp: 4.203 ± 0.068	4.291ProGlu: 4.291 ± 0.071	1.681ProPhe: 1.681 ± 0.039	5.131ProGly: 5.131 ± 0.09	1.178ProHis: 1.178 ± 0.036	2.154ProIle: 2.154 ± 0.044	1.051ProLys: 1.051 ± 0.036	5.035ProLeu: 5.035 ± 0.068	0.846ProMet: 0.846 ± 0.032	0.939ProAsn: 0.939 ± 0.033	2.306ProPro: 2.306 ± 0.053	1.621ProGln: 1.621 ± 0.035	3.788ProArg: 3.788 ± 0.072	3.039ProSer: 3.039 ± 0.069	3.158ProThr: 3.158 ± 0.065	4.822ProVal: 4.822 ± 0.088	0.887ProTrp: 0.887 ± 0.029	1.09ProTyr: 1.09 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.571GlnAla: 3.571 ± 0.066	0.119GlnCys: 0.119 ± 0.011	1.552GlnAsp: 1.552 ± 0.041	1.29GlnGlu: 1.29 ± 0.038	0.779GlnPhe: 0.779 ± 0.031	2.206GlnGly: 2.206 ± 0.051	0.645GlnHis: 0.645 ± 0.024	1.302GlnIle: 1.302 ± 0.041	0.66GlnLys: 0.66 ± 0.028	3.194GlnLeu: 3.194 ± 0.063	0.506GlnMet: 0.506 ± 0.024	0.587GlnAsn: 0.587 ± 0.026	1.559GlnPro: 1.559 ± 0.045	1.106GlnGln: 1.106 ± 0.042	2.531GlnArg: 2.531 ± 0.053	1.385GlnSer: 1.385 ± 0.041	1.465GlnThr: 1.465 ± 0.036	2.219GlnVal: 2.219 ± 0.052	0.456GlnTrp: 0.456 ± 0.021	0.588GlnTyr: 0.588 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.605ArgAla: 9.605 ± 0.14	0.317ArgCys: 0.317 ± 0.02	4.762ArgAsp: 4.762 ± 0.081	4.919ArgGlu: 4.919 ± 0.085	2.486ArgPhe: 2.486 ± 0.047	6.035ArgGly: 6.035 ± 0.097	1.779ArgHis: 1.779 ± 0.043	4.228ArgIle: 4.228 ± 0.083	1.372ArgLys: 1.372 ± 0.038	7.668ArgLeu: 7.668 ± 0.111	1.798ArgMet: 1.798 ± 0.046	1.321ArgAsn: 1.321 ± 0.035	3.964ArgPro: 3.964 ± 0.068	2.06ArgGln: 2.06 ± 0.053	7.435ArgArg: 7.435 ± 0.129	4.022ArgSer: 4.022 ± 0.074	4.358ArgThr: 4.358 ± 0.072	6.264ArgVal: 6.264 ± 0.1	1.222ArgTrp: 1.222 ± 0.032	1.618ArgTyr: 1.618 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.547SerAla: 6.547 ± 0.084	0.257SerCys: 0.257 ± 0.017	2.958SerAsp: 2.958 ± 0.05	2.718SerGlu: 2.718 ± 0.05	1.714SerPhe: 1.714 ± 0.043	5.301SerGly: 5.301 ± 0.072	1.012SerHis: 1.012 ± 0.035	2.293SerIle: 2.293 ± 0.05	1.022SerLys: 1.022 ± 0.039	5.043SerLeu: 5.043 ± 0.061	1.034SerMet: 1.034 ± 0.033	0.943SerAsn: 0.943 ± 0.033	2.955SerPro: 2.955 ± 0.058	1.32SerGln: 1.32 ± 0.037	3.806SerArg: 3.806 ± 0.06	2.969SerSer: 2.969 ± 0.065	3.126SerThr: 3.126 ± 0.059	4.176SerVal: 4.176 ± 0.066	0.926SerTrp: 0.926 ± 0.03	1.105SerTyr: 1.105 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	7.578ThrAla: 7.578 ± 0.109	0.262ThrCys: 0.262 ± 0.019	3.445ThrAsp: 3.445 ± 0.068	3.064ThrGlu: 3.064 ± 0.056	1.815ThrPhe: 1.815 ± 0.041	5.669ThrGly: 5.669 ± 0.092	1.147ThrHis: 1.147 ± 0.031	2.805ThrIle: 2.805 ± 0.056	1.083ThrLys: 1.083 ± 0.039	5.844ThrLeu: 5.844 ± 0.081	0.961ThrMet: 0.961 ± 0.034	1.029ThrAsn: 1.029 ± 0.036	4.024ThrPro: 4.024 ± 0.066	1.47ThrGln: 1.47 ± 0.044	3.77ThrArg: 3.77 ± 0.062	3.098ThrSer: 3.098 ± 0.075	3.593ThrThr: 3.593 ± 0.07	5.484ThrVal: 5.484 ± 0.097	0.934ThrTrp: 0.934 ± 0.033	1.206ThrTyr: 1.206 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	11.821ValAla: 11.821 ± 0.117	0.46ValCys: 0.46 ± 0.023	5.572ValAsp: 5.572 ± 0.086	5.107ValGlu: 5.107 ± 0.073	2.755ValPhe: 2.755 ± 0.056	6.903ValGly: 6.903 ± 0.097	1.701ValHis: 1.701 ± 0.043	4.297ValIle: 4.297 ± 0.076	1.653ValLys: 1.653 ± 0.041	8.824ValLeu: 8.824 ± 0.123	1.454ValMet: 1.454 ± 0.042	1.619ValAsn: 1.619 ± 0.045	4.724ValPro: 4.724 ± 0.072	2.097ValGln: 2.097 ± 0.052	6.277ValArg: 6.277 ± 0.091	4.641ValSer: 4.641 ± 0.075	5.608ValThr: 5.608 ± 0.1	8.449ValVal: 8.449 ± 0.111	1.2ValTrp: 1.2 ± 0.036	1.544ValTyr: 1.544 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.716TrpAla: 1.716 ± 0.048	0.096TrpCys: 0.096 ± 0.011	0.899TrpAsp: 0.899 ± 0.032	0.714TrpGlu: 0.714 ± 0.028	0.579TrpPhe: 0.579 ± 0.025	1.144TrpGly: 1.144 ± 0.041	0.361TrpHis: 0.361 ± 0.021	0.829TrpIle: 0.829 ± 0.028	0.289TrpLys: 0.289 ± 0.018	1.805TrpLeu: 1.805 ± 0.046	0.307TrpMet: 0.307 ± 0.019	0.426TrpAsn: 0.426 ± 0.021	0.727TrpPro: 0.727 ± 0.027	0.582TrpGln: 0.582 ± 0.025	1.38TrpArg: 1.38 ± 0.046	0.832TrpSer: 0.832 ± 0.029	0.932TrpThr: 0.932 ± 0.032	1.109TrpVal: 1.109 ± 0.033	0.363TrpTrp: 0.363 ± 0.019	0.275TrpTyr: 0.275 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.595TyrAla: 2.595 ± 0.046	0.127TyrCys: 0.127 ± 0.011	1.394TyrAsp: 1.394 ± 0.04	1.238TyrGlu: 1.238 ± 0.036	0.66TyrPhe: 0.66 ± 0.025	1.908TyrGly: 1.908 ± 0.044	0.29TyrHis: 0.29 ± 0.018	0.685TyrIle: 0.685 ± 0.026	0.353TyrLys: 0.353 ± 0.019	2.026TyrLeu: 2.026 ± 0.045	0.29TyrMet: 0.29 ± 0.016	0.437TyrAsn: 0.437 ± 0.024	1.018TyrPro: 1.018 ± 0.041	0.517TyrGln: 0.517 ± 0.024	1.654TyrArg: 1.654 ± 0.044	1.042TyrSer: 1.042 ± 0.033	1.155TyrThr: 1.155 ± 0.035	1.625TyrVal: 1.625 ± 0.043	0.306TyrTrp: 0.306 ± 0.017	0.434TyrTyr: 0.434 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3006 proteins (972793 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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